• Mycobiology
• /
• 제40권1호
• /
• pp.8-13
• /
• 2012

In the present study, an attempt to evaluate the antimicrobial and antioxidant activity of fungal endophytes inhabiting $Emblica$ $officinalis$ has been made keeping in view the medicinal importance of the selected host plant in Indian traditional practices. A total of four endophytic fungi belonging to Phylum Ascomycetes were isolated from different parts of the plant which were characterized morphologically and by using rDNA-internal transcribed spacer. The most frequently isolated endophyte was $Phomopsis$ sp. The antioxidant activity by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and reducing power assay, and total phenol were evaluated using ethanolic extract of endophytic fungi. DPPH activities in all the ethanolic extract increased with the increase in concentrations. Endophytes, $Phomopsis$ sp. and $Xylaria$ sp. showed highest antioxidant activity and also had the higher levels of phenolics. Antimicrobial activity of fungal extract were tested against four bacteria namely, $Escherichia$ $coli$ MTCC730, $Enteroccocus$ $faecalis$ MTCC2729, $Salmonella$ $enterica$ ser. $paratyphi$ MTCC735 and $Streptococcus$ $pyogenes$ MTCC1925, and the fungus $Candida$ $albicans$ MTCC183. In general, the fungal extracts inhibited the growth of test organisms except $E.$ $coli$.

• The Plant Pathology Journal
• /
• 제35권6호
• /
• pp.598-608
• /
• 2019

Endophytic fungi have received much attention as plant growth promoters as well as biological control agents against many plant pathogens. In this study, 30 endophytic fungal species, isolated from various plants in China, were evaluated using in vitro dual culture assay against Fusarium oxysporum f. sp. cucumerinum, causing wilt in cucumber. The results of the present study clearly showed that all the 30 endophytic fungal isolates were highly capable of inhibiting the mycelial colony growth of Fusarium oxysporum f. sp. cucumerinum with inhibition % over 66% as compared to control treatments. Among all of them, 5 isolates were highly effective such as, Penicillium sp., Guignardia mangiferae, Hypocrea sp., Neurospora sp., Eupenicillium javanicum, and Lasiodiplodia theobromae, respectively. The Penicillium sp. and Hypocrea sp. were highly effective as compared to other isolates. From in vitro results 10 best isolates were selected for greenhouse studies. The results of the greenhouse studies showed that among all of them 3 endophytic fungal isolates successfully suppressed wilt severity when co-inoculation with pathogen Fusarium. oxysporum f. sp. cucumerinum. The endophytic fungi also enhanced plant growth parameters of the host plants, the antagonistic fungal isolates increased over all plant height, aerial fresh, and dry weight as compared to control.

• Journal of Microbiology and Biotechnology
• /
• 제31권11호
• /
• pp.1526-1532
• /
• 2021

Suaeda australis, Phragmites australis, Suaeda maritima, Suaeda glauca Bunge, and Limonium tetragonum in the Seocheon salt marsh on the west coast of the Korean Penincula were sampled in order to identify the endophytes inhabiting the roots. A total of 128 endophytic fungal isolates belonging to 31 different genera were identified using the fungal internal transcribed spacer (ITS) regions and the 5.8S ribosomal RNA gene. Fusarium, Paraconiothyrium and Alternaria were the most commonly isolated genera in the plant root samples. Various diversity indicators were used to assess the diversity of the isolated fungi. Pure cultures containing each of the 128 endophytic fungi, respectively, were tested for the plant growth-promoting abilities of the fungus on Waito-C rice germinals. The culture filtrate of the isolate Lt-1-3-3 significantly increased the growth of shoots compared to the shoots treated with the control. Lt-1-3-3 culture filtrate was analyzed and showed the presence of gibberellins (GA₁ 2.487 ng/ml, GA₃ 2.592 ng/ml, GA₉ 3.998, and GA₂₄ 6.191 ng/ml). The culture filtrate from the Lt-1-3-3 fungal isolate produced greater amounts of GA₉ and GA₂₄ than the wild-type Gibberella fujikuroi, a fungus known to produce large amounts of gibberellins. By the molecular analysis, fungal isolate Lt-1-3-3 was identified as Gibberella intermedia, with 100% similarity.

• 한국균학회지
• /
• 제46권3호
• /
• pp.205-211
• /
• 2018

Fungal endophytes are defined as fungi inhabiting plant tissues, causing no apparent disease. As the agricultural and ecological importance of endophytes has increased, many studies have been performed on various aspects, ranging from basic identification, taxonomy, and evolution to secondary metabolites with potential for human use. In the Korean Peninsula, conifers are the most commonly found evergreen trees, with approximately 30 to 60 reported taxa. Studies on the effect of conifer-associated endophytes on the host plants are required for the preservation and conservation of coniferous forests which decline by climate change and deforestation. This review summarizes the diversity of endophytic fungi in coniferous trees of Korea and their relationship with host plants.

• Mycobiology
• /
• 제49권1호
• /
• pp.89-94
• /
• 2021

Forty-three (n = 43) endophytic fungi with different morphologic characteristics were from a medicinal plant Sceletium tortuosum, were utilized to investigate their antifungal effectiveness against pathogenic fungi. All fungal isolates exhibited antifungal activity against one or more pathogens in the dual culture test whereas only 33 fungal culture filtrates (77%) showed decent antifungal effect. Fusaria and Aspergillus were the dominate genus that displayed significant antifungal activity. Isolates GG02, GG09, ND15, and ND17 showed the broadest spectrum of antifungal activity. Furthermore, culture filtrate of Fusarium sp. DR08 exhibited a broad range of antifungal activity against all the pathogens. The results suggest endophytic fungi isolated from medicinal plant might be a source of novel bioactive molecules. To the best our knowledge, this is the first report on endophytic fungi isolated from native kougoed exhibiting antifungal activity against plant fungal pathogens.

• Mycobiology
• /
• 제43권4호
• /
• pp.373-383
• /
• 2015

Five halophytic plant species, Suaeda maritima, Limonium tetragonum, Suaeda australis, Phragmites australis, and Suaeda glauca Bunge, which are native to the Muan salt marsh of South Korea, were examined for fungal endophytes by sequencing the internal transcribed spacer (ITS) region containing ITS1, 5.8S rRNA, and ITS2. In total, 160 endophytic fungal strains were isolated and identified from the roots of the 5 plant species. Taxonomically, all 160 strains belonged to the phyla Ascomycota, Basidiomycota, and Zygomycota. The most dominant genus was Fusarium, followed by the genera Penicillium and Alternaria. Subsequently, using 5 statistical methods, the diversity indices of the endophytes were determined at genus level. Among these halophytic plants, P. australis was found to host the greatest diversity of endophytic fungi. Culture filtrates of endophytic fungi were treated to Waito-C rice seedlings for plant growth-promoting effects. The fungal strain Su-3-4-3 isolated from S. glauca Bunge provide the maximum plant length (20.1 cm) in comparison with wild-type Gibberella fujikuroi (19.6 cm). Consequently, chromatographic analysis of the culture filtrate of Su-3-4-3 showed the presence of physiologically active gibberellins, $GA_1$ (0.465 ng/mL), $GA_3$ (1.808 ng/mL) along with other physiologically inactive $GA_9$ (0.054 ng/mL) and $GA_{24}$ (0.044 ng/mL). The fungal isolate Su-3-4-3 was identified as Talaromyces pinophilus.

• 한국균학회지
• /
• 제51권4호
• /
• pp.335-347
• /
• 2023

Sordariomycetes is the second largest class of Ascomycota, distributed throughout various habitat including terrestrial and aquatic environments and also existing as endophytes. We isolated endophytic fungal strains in Korea, identifying them based on their morphological characteristics and molecular analyses, using eight specific DNA regions for accurate genus identification. We identified five previously unrecorded endophytic fungal species in Korea: Chaetomium subaffine, Colletotrichum jiangxiense, Colletotrichum sydowii, Diaporthe vacuae and Neurospora tetraspora. In this study, we describe the morphological characteristics and present our phylogenetic analyses of these five fungal species.

• 한국균학회지
• /
• 제44권4호
• /
• pp.252-258
• /
• 2016

본 연구는 제주도 사려니숲과 동백동산에서 편백나무(Chamaecyparis obtusa), 삼나무(Cryptomeria japonica), 비자나무(Torreya nucifera), 꽝꽝나무(Ilex crenata), 동백나무(Camellia japonica) 등 5종의 목본 식물의 잎에서 내생균을 분리하고, 형태 및 분자생물학적 특징 통해 동정하였다. 이를 위해 primer인 ITS1F와 ITS4를 이용하여 internal transcribed spacer (ITS) rDNA영역과 primer LR0R과 LR16을 이용하여 large subunit rDNA 영역을 그리고 primer Bt2a과 Bt2b를 이용하여 ${\beta}$-tubulin 영역의 염기서열을 동시에 분석하였다. 그 결과 Mycosphaerella aleuritidis, Neofusicoccum eucalyptorum, Neofusicoccum parvum, Phyllosticta citrichinensis, Phyllosticta cryptomeriae, Phomopsis cotoneastri, Sphaerulina rhododendricola, Guignardia mangiferae, Lophodermium jiangnanense, Lophodermium minus 등 10종의 미기록 내생균을 동정하였고, 이를 보고하고자 한다.

• Mycobiology
• /
• 제48권3호
• /
• pp.240-244
• /
• 2020

Alternaria alternata JS-1623 is an endophytic fungus isolated from a stem tissue of Korean fir, Abies koreana. Ethyl acetate extracts of culture filtrates exhibited anti-inflammatory activity in LPS induced microglia BV-2 cell without cytotoxicity. Here we report a 33.67 Mb sized genome assembly of JS-1623 comprised of 13 scaffolds with N50 of 4.96 Mb, and 92.41% of BUSCO completeness. GC contents were 50.97%. Of the 11,197 genes annotated, gene families related to the biosynthesis of secondary metabolites or transcription factors were identified.

• 한국균학회지
• /
• 제43권2호
• /
• pp.133-136
• /
• 2015

We investigated endophytic fungi diversity of two Pinaceae species (Pinus densiflora, Pinus rigida) and one Cupressaceae species (Juniperus rigida) on Mt. Seodae, Korea. In total, 34 isolates were obtained from 19 host plants and identified according to internal transcribed spacer (ITS) region. As a result, they were placed in 13 taxa. Among them, 52.9% belonged to Leotiomycetes, 32.3% belonged to Sordariomycetes, 5.8% belonged to Agaricomycetes, and 3.0% for isolates belonged to either Eurotiomycetes, Dothideomycetes or Ustilaginomycetes. In particular, Lophodermium growing in pine would be an especially important instance of biodiversity for endophytes, suggesting that further study examining its ecological function in the environment is critical.