• Journal of Microbiology and Biotechnology
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• v.18 no.7
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• pp.1308-1316
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• 2008

Propionibacterium acnes is known to playa pivotal role in the pathogenesis of acne vulgaris. CBT-SL5 is one of the antimicrobial peptides from Enterococcus faecalis SL5, and it has shown antimicrobial activity against P. acnes. The aim of this study was to investigate the anti-inflammatory effect of CBT-SL5 on the inflammation induced by P. acnes in cultured human keratinocyes. Cultured human keratinocytes derived from neonatal foreskin were treated with heat-killed P. acnes to induce inflammation, and then various concentrations of CBT-SL5 were added to the P. acnes-treated keratinocytes. The mRNA expression and protein secretion of interleukin (IL)-8, an inflammation marker, was analyzed by real-time reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. We also analyzed the nuclear factor-kappa B (NF-$\kappaB$) p65 translocation by performing immunofluorescent staining. P. acnes treatment up regulated the IL-8 mRNA expression in the keratinocytes, and this was brought about through both toll-like receptor (TLR)2 and TLR4. At the concentrations of 10, 50, and 100 ng/ml, CBT-SL5 significantly down regulated the P. acnes-induced IL-8 mRNA expression and protein production (p<0.05). At 6 hand 12 h of the treatment, CBT-SL5 significantly suppressed the P. acnes-induced IL-8 mRNA expression. Secretion of IL-8 protein was significantly reduced at 24 h. The functional inhibitory activity of CBT-SL5 was shown by CBT-SL5 suppressing the P. acnes-induced NF-$\kappaB$ translocation from the cytoplasm to the nucleus. These results demonstrated that CBT-SL5 suppressed the P. acnes-induced IL-8 expression in keratinocytes. Therefore, CBT-SL5 may be a novel anti-inflammatory treatment for acne.

• Archives of design research
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• v.17 no.1
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• pp.329-338
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• 2004

This study did derive a characteristic of a cover design through considering a concept, a function and components of a cover design based on a literature study and through analyzing cases of the best-seller cover designs for the literature section from 1999 to 2002. In terms of a reader's preference for a cover design according to an age and sex of a reader, it tried to suggest a condition and a method of a book-cover design through a questionnaire survey and the image evaluation. In a survey of the preference for a cover design, there was the difference of interest degree in a kind of a book, a place to buy a book and a cover design according to an age and sex. In addition, it was shown that a woman prefers the stylish typography, and both a man and a woman prefer the cool color. However, except these two, the reason why there is no difference in preference for visual elements are that a cover design is an integrative image comprising several elements. Accordingly, through the Semantic Differential Method, it carried out the evaluation as to a cover design as an integrative image. As a result of that, an abstract image was shown to be difficult to convey a meaning because of its fall in clarity. Further, a cover design mainly comprising the stylish typography gained the high evaluation in unique and clarity. It is thought to be attributable to the easiness to achieve a goal of seeing and reading in a cover design in which an image and the typography are balanced, rather than the excessive visual. Even though the visual is excellent, there was difference from what having been excellent in conveying a meaning, which is a functional aspect. This study tried to examine, limiting it only to a visual element. It will be hereafter required to proceed with a study on the influence on a reader's preference according to material, bookbinding and a form of book which are elements comprising a cover design.

• Journal of Biomedical Engineering Research
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• v.24 no.4
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• pp.267-273
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• 2003

The present study attempted to observe what changes the supply of highly concentrated (30%) oxygen cause to people's ability of visuospatial cognition, compared to air of normal oxygen concentration (21%). This study sampled eight male university students (the average age : 23.5) as subjects for functional Magnetic Resonance Imaging (MRI) study It also developed equipment that supplies 21% and 30% oxygen) at a constant rate of 8L/min. Two questionnaires containing 20 questions were developed to measure the ability of visuospatial cognition, and accuracy was calculated from the result of task performance. The experiment paradigm consisted of the run conducting tasks at 30%'s concentration of oxygen and another run at 21%'s concentration of oxygen. Each run was composed of four blocks and each block included eight control tasks and five visuospatial taks. 3T MRI was used and fMRI was obtained through the single-shot EPI method. The activation in the occipital-associated area, bilateral superior parietal lobes, bilateral inferior parietal lobes. bilateral precuneus, bilateral postcentral gyri, bilateral middle frontal gyri, bilateral inferior frontal gyri, bilateral medial frontal gyri, bilateral superior frontal gyri, bilateral cingulate gyri was significantly increased at the 30%'s concentration of oxygen rather than 21%'s. Furthermore, the result of task performance showed the accuracy increased at 30%'s concentration of oxygen rather than 21%'s. From the result of this study, it is concluded that the supply of highly concentrated oxygen has a positive effect on the ability of visuospatial cognition.

• The Korean Journal of Pesticide Science
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• v.11 no.3
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• pp.164-170
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• 2007

The concept of innate immunity in insects which refers to the first line of host defense constitutes the humoral and cellular components which are involved in recognition and actively participate in the elimination of the intruding foreign micro- or macro-organisms. Several recent studies suggest that juvenile hormone (JH) modulates the cellular immune reactions in response to pathogen. In this study, pyriproxyfen (a JH agonist as an insect growth regulator) was tested in its any inhibitory effect on the immune reactions of the beet armyworm, Spodoptera exigua. To this end, five different hemocyte morphotypes of final instar S. exigua were identified by phase contrast microscopy. Plasmatocytes and granular cells, which constitute about 90% of the total hemocyte count, were prominently distinguished based on their basophilic/acidophilic nature using Giemsa stain. The role of pyriproxyfen on the functional ability of hemocytes was analyzed using FITC-labeled Providencia vermicola for the phagocytic potential of the hemocytes. Both granular cells and plasmatocytes exhibited phagocytosis behavior. Pyriproxyfen significantly inhibited the phagocytosis of both cell types, proposing its novel action as an immunosuppressant.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.2
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• pp.116-122
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• 2017

The environmental lighting of a city advances its landscape standards and the city's touristic value because the lighting can help improve the city's safety and security as well as its esthetic value. The types of parks in cities vary in size and function and should be reflected carefully in lighting planning to gain the best effect. The importance of city lighting has been increased in recent years due to the expanded span of urban night life. Lighting systems around rivers and coastal waterfronts in cities play an important role not only in their functional aspects, such as safety and security, but also in boosting the city's identities. This paper performed comparison analysis among Tsim Sha Tsui Promenade in Hong Kong, Fukuoka Nakagawa Park in Japan, and the walking path in Hamdeok Beach in Jeju City, Korea. According to the evaluation, Hong Kong is excellent in 8 evaluation criteria for park lighting so that the city shows its superior installation and management. Fukuoka is also good by being analyzed as 'fair' in 5 criteria. Finally, Jeju is judged as inferior overall by showing 'poor' in several criteria. The paper shows how they differ in their environment lighting characteristics and aims to suggest stable lighting planning strategies for seaside parks in Jeju so the citizens and visitors of Jeju can enjoy the beautiful sceneries and nighttime activities.

• Applied Biological Chemistry
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• v.41 no.7
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• pp.527-532
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• 1998

This study was aimed at the development of the rice product by fermentation of saccharified rice solution with Bifidobacterium. To optimize the preparation of saccharified rice solution for Bifidobacterium fermentation, various pretreatment conditions were established. Grinding for 30seconds by an impact mill was more efficient than any other grinding schemes tested. The preheating before gelatinization showed a positive effect for efficient saccharification, and its optimal conditions were at $60^{\circ}C$ for 45 min. The optimum gelatinization conditions were at $100^{\circ}C$ for 40 min. The optimum levels of enzymes for saccharification of rice were 0.135 unit/g rice powder for ${\alpha}-amylase$ and 3.375 unit/g rice powder for glucoamylase, respectively. The physico-chemical properties of the fermented product by a fastidious Bifidobacterium showed a great potential for a functional rice product. However, an improvement on its flavor was required, which might be achieved by the addition of various fruits and vegetables.

• Applied Biological Chemistry
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• v.49 no.3
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• pp.233-237
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• 2006

In the present study, we compared the functional analysis of pear extracts cultured in conventional and environment-friendly conditions in primary cultured rat hepatocytes. ATP synthesis significantly increased by the treatment with environment friendly cultured pear powder but not by conventional group. In addition, cell proliferation using $[^3H]$-thymidine incorporation was also stimulated by environment-friendly cultured pear extract compared to conventional group. Moreover, the expressions of CDK-2 and CDK-4 were increased but p21WAF1/Cip1 and p27 Kip1 decreased by environment-friendly cultured pear extract but not by conventional group. In conclusion, environment-friendly cultured pear powder has stimulatory effect on cell proliferation compared to conventional group in primary cultured rat hepatocytes.

• Journal of Applied Biological Chemistry
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• v.55 no.2
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• pp.117-121
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• 2012

Biological activities of the hot water and ethanol extracts from Opuntia humifusa cladodes were investigated. 1,1-diphenyl-2-picryl hadrazyl (DPPH) electron donating ability of hot water and ethanol extracts was 79.07 and 82.54%, respectively. Hot water extract generally showed better cytotoxic activity than ethanol extract against each cell line. HeLa and AGS cell lines treated with hot water extract had more than 50% cytotoxic activities. Based on the antimicrobial activities against four microbial strains, both extracts inhibited growth of Staphylococcus aureus KCCM 11593, whereas affected cell growth of three other microorganisms, Escherichia coli (KCCM 11234), Pseudomonas aeruginosa (ATCC 27853), and Salmonella typhimurium (ATCC 11862), in proportion to the concentration of extracts. The inflammatory activities against hot water extract (34.31%) showed higher than that of ethanol extract (25.59%). The effect of extracts on 3T3-L1 preadipocytes differentiation showed that differentiation of treated group with 80 and 100 ${\mu}g/mL$ of hot and ethanol extracts were increased more than treated group with isobutyl methyl xanthine (IBMX) + dexamethasone. These results indicate that the O. humifusa cladodes extracts can be used as a functional material due to their effective biological activities.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2017.10a
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• pp.223-226
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• 2017

With the development of information technology, users are provided with information they want through mobile device in various situations. When users communicate with information, there is interaction through gesture activities such as tapping and experience in the process. Experience through interaction in mobile affect the user's psychology. This is important because it is related to the behavior of the user in the future. Various types of information presentation methods have been researched in mobile environment. However, there are more research focusing on functional interactivity. The purpose of this study is to investigate the effect of interaction and usage mode on satisfaction, usefulness, and intention to use for sound text presentation that is user-centered. As variables for my study, there are two factors which are interactivity and usage mode. The interactivity type is composed of two ways that are: High and Low depending on modality and message interactivity; and the usage mode is composed of Action mode and Goal mode depending on whether user has a task or not. The experimental design is $2{\times}2$. The same content is provided in (a) only Modality interactivity, and (b) Modality and Message interactivity are provided. Depending on usage mode, (a) Action mode is processed without a specific task, and (b) Goal mode is performed with a specific task to participants. The experimental study demonstrated that there is a difference in satisfaction, usefulness, and intention to use depending on the difference of interaction and usage mode when providing information in mobile environment. The results of this study are summarized as follows: interaction and usage mode have significant influence on mobile user's satisfaction, usefulness, intention to use.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.5
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• pp.522-529
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• 2016

This study examined the effects of 4-weeks of backward walking exercise on ROM, VAS (Visual Analogue Scale) score and proprioception in anterior cruciate reconstruction patients. Fourteen subjects were randomly assigned to either a backward walking group (BWG, n=7) or a control group (CON, n=7) and then participated in rehabilitation program for 4 weeks. The ROM of the flexion significantly decreased in both groups (BWG, p<0.001 vs CON, p<0.01), while ROM of extension significantly decreased only in the BWG (p<.05) post exercise, but no significant difference was observed between groups. Additionally, VAS score decreased significantly in both groups (P<0.001) and BWG was lower than CON post exercise (p<0.001). Finally, proprioception decreased significantly in both groups (BWG, p<0.001 vs CON, p<0.05), but did not differ significantly between groups. These results suggest that the 4 week backward walking rehabilitation program improves ROM, VAS score, and proprioception and has positive effects on functional recovery of anterior cruciate ligament reconstruction patients.