• 한국도로학회논문집
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• 제20권1호
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• pp.35-45
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• 2018

PURPOSES : This study examines the performance changes of road networks according to the strength of a disaster, and proposes a method for estimating the quantitative resilience according to the road-network performance changes and damage scale. This study also selected high-influence road sections, according to disasters targeting the road network, and aimed to analyze their hazard resilience from the network aspect through a scenario analysis of the damage recovery after a disaster occurred. METHODS : The analysis was conducted targeting Sejong City in South Korea. The disaster situation was set up using the TransCAD and VISSIM traffic-simulation software. First, the study analyzed how road-network damage changed the user's travel pattern and travel time, and how it affected the complete network. Secondly, the functional aspects of the road networks were analyzed using quantitative resilience. Finally, based on the road-network performance change and resilience, priority-management road sections were selected. RESULTS : According to the analysis results, when a road section has relatively low connectivity and low traffic, its effect on the complete network is insignificant. Moreover, certain road sections with relatively high importance can suffer a performance loss from major damage, for e.g., sections where bridges, tunnels, or underground roads are located, roads where no bypasses exist or they exist far from the concerned road, including entrances and exits to suburban areas. Relatively important roads have the potential to significantly degrade the network performance when a disaster occurs. Because of the high risk of delays or isolation, they may lead to secondary damage. Thus, it is necessary to manage the roads to maintain their performance. CONCLUSIONS : As a baseline study to establish measures for traffic prevention, this study considered the performance of a road network, selected high-influence road sections within the road network, and analyzed the quantitative resilience of the road network according to scenarios. The road users' passage-pattern changes were analyzed through simulation analysis using the User Equilibrium model. Based on the analysis results, the resilience in each scenario was examined and compared. Sections where a road's performance loss had a significant influence on the network were targeted. The study results were judged to become basic research data for establishing response plans to restore the original functions and performance of the destroyed and damage road networks, and for selecting maintenance priorities.

• Clinics in Shoulder and Elbow
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• 제18권2호
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• pp.68-74
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• 2015

Background: The purpose of our study was to evaluate the functional and radiologic outcomes of additive augmentation sutures through rotator cuff for proximal humeral fractures stabilized locking plate in elderly patients. Methods: We enrolled 74 patients over the age of 60 years who received internal fixation using locking plates for proximal humeral fractures. Of these, 50 patients had additive augmentation sutures through rotator cuff. The mean age at the time of surgery was 72.1 years (range, 60-89 years), and the mean follow-up period was 17.5 months (range, 12-62 months). The humeral neck-shaft angle and humeral head height were used as radiological markers to assess the effect of additive augmentation sutures through rotator cuff. We allocated the patients who received additive augmentation sutures into group A and those who did not into group B. Results: At the final follow-up, the mean Korean Showlder Society score and Constant scores were $88.96{\pm}12.1$ and $86.6{\pm}11.9$, respectively, in group A and $86.21{\pm}11.8$ and $85.3{\pm}11.7$, respectively, in group B (p=0.368, 0.271). At the final follow-up, the mean loss in humeral neck-shaft angle from the time of immediate postoperative measurement was $1.6^{\circ}$ in group A and $4.8^{\circ}$ in group B, whereas the mean loss in humeral head height was 0.82 mm in group A and 0.52 mm in group B (p=0.029, 0.178). Conclusions: The surgical outcomes of internal fixation using locking plates for proximal humeral fractures were clinically and radiologically good in elderly patients over the age of 60 years without any observable complications. Further, the loss of humeral head shaft angle at the final follow-up from its initial postoperative measurement was significantly smaller in patients who received an additive augmentation suture than in those who did not. Thus, we conclude that augmentation sutures are a beneficial option for elderly patients that clinicians can consider at the time of surgical decision making.

• 한국유기농업학회:학술대회논문집
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• 한국유기농학회 2009년도 하반기 학술대회
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• pp.103-113
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• 2009

유기농 및 일반재배 대두를 다양한 용매에서 추출하여 그 추출물에 대한 항산화 효과, 항염증 효과, 대식세포의 세포사멸 보호 효과, 그리고 알레르기 저해효과를 비교하였다. DPPH 법과 SOD 효소의 활성 측정으로 항산화 능력을 평가하였으며, 항염증 효과는 LPS로 유도시킨 Raw 264.7 세포주에서 염증 매개 단백질인 Cox-2와 iNOS의 발현량을 western blotting으로 조사하여 분석하였다. 또한 대식세포 보호 효과를 세포사멸 조절 단백질인 Bcl-xl의 발현량 조사로 평가하였고, 1 mM $H_2O_2$ 처리로 유도되는 세포사멸의 보호효과를 세포 수 측정법으로 조사하였다. 항알레르기 효과는 흰쥐의 비만세포인 RBL-2H3 세포주를 사용하여 IgE로 매개되는 비만세포의 탈과립화를 OPT assay로 측정하여 분석하였다. 그 결과, 일반 재배보다 유기 재배 대두 추출물이 더 좋은 항산화, 세포사멸 보호, 항염증 효과를 나타내었다. 특히 유기농 대두의 열수 추출물은 LPS로 유도시킨 염증반응을 효과적으로 억제하는 것으로 나타났다. 그러나 항알레르기 효과는 모든 추출물에서 히스타민 유리량이 약간 감소하는 것으로 나타나 유기농과 일반재배의 차이를 확인할 수 없었다. 대두에는 많은 유용한 생리활성물질 뿐 만 아니라 알레르기 유발 물질도 존재하는 것으로 알려져 있지만 조추출물 상태에서는 특이적인 알레르기 유발보다 오히려 항알레르기 효과가 나타났다. 결론적으로, 만성 염증 및 천식, 그리고 아토피피부염과 같은 염증관련 질환에는 유기재배된 대두가 일반 대두보다 좋은 억제 효과를 가지고 있다고 판단된다.

• 동의생리병리학회지
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• 제22권3호
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• pp.684-691
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• 2008

As part of studies to develop new materials to lower blood glucose levels using crude polysaccharide, this study was attempted to analyze the characteristics of crude polysaccharide obtained from the extracts of a mixed herbal medium(OCM) where Trichloloma matsutake mycelium and Cordyceps militaris mycelium were cultured together and to look into the influence of administering these by concentration upon the blood glucose and serum lipid levels of rats with diabetes which was induced by STZ(Streptozotosin). Experimental group was divided into 6 groups: first, it was divided into normal control group(NC group) and diabetes-induced group, and diabetes-induced group was subdivided into diabetic control group(DC group), acarbose-treated group(PC group), 100 mg/kg/body weight-treated by crude polysaccharide of OCM(UE) group(UE100 group), 200 mg/kg/body weight-treated group(UE200 group), and 300 mg/kg/body weight-treated group(UE300 group). In diabetic-induced groups, after streptozotocin was melted in 0.01M citrate buffer at 50 mg/kg/body weight, when the non-fasting blood glucose level not on an empty stomach was 300 mg/dl or more in blood collected from the tail vein, it was regarded as diabetic induction and then such diabetic-induced experimental animals were used in this experiment. The yield of crude polysaccharide obtained from OCM was found to be 0.31% and the ${\beta}$-glucan content 39.40%. As a result of analyzing NO on immune function, which is known as major physiological activity of crude polysaccharide, high NO viability was shown; when 1 mg/ml LPS was treated at 1 ug/ml, it was found to be 50.77 uM, and when LPS was treated at 10 ug/m, it was found to be 53.78 uM. Also, regarding cancer cells, cell count was decreased by about 26% in proportion to sample concentration, while for normal cells, it was a little decreased in proportion to concentration, however, cell count was maintained in the range of $81.92{\sim}98.16%$ at all concentrations. In case of blood glucose level, it was decreased in all extract-treated groups compared to DC group and in the cases of ALT and AST, they were found to be lower in extract-treated groups compared to PC group and for serum lipid, it was found to be lower in UE100 group compared to PC group. Thus this study tried to utilize these results as fundamental data for development of preventive and therapeutic agents against diabetes as well as functional foods using the crude polysaccharide of mushrooms.

• Journal of Plant Biotechnology
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• 제39권4호
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• pp.281-287
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• 2012

본 연구에서는 용암해수가 지닌 독특한 미네랄 구성을 바탕으로 자란의 생육에 적합한 농도를 규명하기 위해 실험을 수행하였다. 자란의 기내배양용 고체배지구성을 위해 30 g/L sucrose와 3.0과 8.0 g/L의 gelrite 및 plant agar를 동일하게 처리한 MS 배지와 RO(Reverse Osmosis) 탈염수, ED(Electrodialsis) 탈염수 배지를 각각 제조하여 배지경도를 측정한 결과, plant agar가 첨가된 MS, RO, 및 ED 배지의 경도는 각각 60,000, 40,000 및 $38,000g/cm^2$의 경도가 측정되었다. 하지만 gelrite가 첨가된 배지는 MS를 제외한 RO, ED배지는 $2,000g/cm^2$ 내외의 경도를 보였다. 제조된 배지에서 식물체의 생육을 확인한 결과, RO 탈염수 0%에서는 88%, RO 50%에서는 57%, RO 100%에서는 31%의 생존율을 보였다. 하지만 잎 길이, 엽폭은 모두 RO 탈염수 100%에서 높았다. ED배지는 ED 100%를 제외한 배지에서 모두 양호한 생장을 보였다. 엽록소 함량 분석을 통한 생리적 건전도를 측정한 결과, 대조구에서는 총엽록소 함량이 66.3 ug/ml인데 비해 RO 10%, RO 50%, RO 100%, ED 10%, ED 50%, ED 100%에서는 각각 77.3, 69.3, 76.4, 79.0, 75.7, 74.4 ug/ml로 모두 대조구보다 높은 총엽록소 함량을 나타냈다. 또한 용암해수 내 유용성분의 식물체내로의 축적은 스토론튬, 바나듐, 셀레늄 등이 원활하게 이루어짐을 확인한 바, 용암해수를 이용한 식물조직배양용 배지로의 산업적 이용가치가 높을 것으로 보이고 더 나아가 용암해수가 원예작물뿐만 아니라 유용성분 함유 약용작물의 생산에도 충분히 활용 가능할 것으로 사료된다.

• The Plant Pathology Journal
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• 제32권2호
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• pp.112-122
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• 2016

Virus-induced gene silencing (VIGS) is an effective tool for the study of soybean gene function. Successful VIGS depends on the interaction between virus spread and plant growth, which can be influenced by environmental conditions. Recently, we developed a new VIGS system derived from the Soybean yellow common mosaic virus (SYCMV). Here, we investigated several environmental and developmental factors to improve the efficiency of a SYCMV-based VIGS system to optimize the functional analysis of the soybean. Following SYCMV: Glycine max-phytoene desaturase (GmPDS) infiltration, we investigated the effect of photoperiod, inoculation time, concentration of Agrobacterium inoculm, and growth temperature on VIGS efficiency. In addition, the relative expression of GmPDS between non-silenced and silenced plants was measured by qRT-PCR. We found that gene silencing efficiency was highest at a photoperiod of 16/8 h (light/dark) at a growth temperature of approximately $27^{\circ}C$ following syringe infiltration to unrolled unifoliolate leaves in cotyledon stage with a final SYCMV:GmPDS optimal density $(OD)_{600}$ of 2.0. Using this optimized protocol, we achieved high efficiency of GmPDS-silencing in various soybean germplasms including cultivated and wild soybeans. We also confirmed that VIGS occurred in the entire plant, including the root, stem, leaves, and flowers, and could transmit GmPDS to other soybean germplasms via mechanical inoculation. This optimized protocol using a SYCMV-based VIGS system in the soybean should provide a fast and effective method to elucidate gene functions and for use in large-scale screening experiments.

• Korean Journal of Acupuncture
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• 제21권2호
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• pp.29-46
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• 2004

Objectives and Methods : This study was performed to investigate the effect of electro -acupuncture at four gates(bilateral LR3 and LI4) on brain activity in normal subjects using fMRI. Results and Conclusions : 1. fMRI signal increase by electro-acupuncture at Lt. LR3 was observed in Rt. Middle frontal gyrus in group average as well as more than half of the subjects. 2. fMRI signal decreases by electro-acupuncture at Lt. LR3 were observed in Rt. Superior frontal gyrus, Rt. Middle temporal gyrus, Rt. Cingulate gyrus in group average as well as more than half of the subjects. 3. fMRI signal increases by electro-acupuncture at Lt. LI4 were observed in Lt. Superior frontal gyrus, Lt. Middle frontal gyrus, Lt. Inf. Semi-Lunar Lobule(cerebellum), Rt. Middle frontal gyrus, Rt. Cingulate gyrus in group average as well as more than half of the subjects. 4. fMRI signal decreases by electro-acupuncture at Lt. LI4 were observed in Lt. Middle frontal gyrus, Lt. Inferior frontal gyrus, Lt. Precentral gyrus and Rt. Middle frontal gyrus, Rt. Middle temporal gyrus, Rt. Precuneus, Rt. Inferior frontal gyrus, Rt. Postcentral gyrus in group average as well as more than half of the subjects. 5. fMRI signal increase by electro-acupuncture at Lt. LR3 and Lt. LI4 in group average as well as more than half of the subjects was not observed. 6. fMRI signal decreases by electro-acupuncture at Lt. LR3 and Lt. LI4 were observed in Lt. culmen(cerebellum), Lt. Cingulate gyrus와 Rt. Middle frontal gyrus, Rt. Cingulate gyrus, Rt. Inferior frontal gyrus in group average as well as more than half of the subjects. 7. fMRI signal increases by electro-acupuncture at four gates (bilateral LR3 and LI4) were observed in Lt. Middle temporal gyrus and Lt. Postcentral gyrus in group average as well as more than half of the subjects. 8. fMRI signal decrease by electro-acupuncture at four gates (bilateral LR3 and LI4) were observed in Lt. Middle frontal gyrus, Lt. Precentral gyrus, Lt. Inferior frontal gyrus, Lt. Middle temporal gyrus, Lt. Frontal sub-gyral and Rt. Tuber(cerebellum) in group average as well as more than half of the subjects.

• 한국잡초학회지
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• 제30권3호
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• pp.243-251
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• 2010

피복작물을 이용한 잡초 방제효과와 녹비효과를 비교 분석하여 효과적인 피복작물-잡곡 작부체계를 알아보기 위한 호밀과 자운영을 이용하여 포장시험을 수행한 결과는 다음과 같다. 피복작물의 잡초 방제효과는 잡초의 발아억제와 생육저해로 잡곡 초기생장에 있어 잡초발생시기를 늦추었다. 그러나 잡초 방제기간이 자운영은 30일 내외, 호밀은 40일 내외로 자운영보다 호밀의 잡초방제 효과가 뛰어난 것으로 나타났다. 무처리구와 자운영 피복구는 잡곡의 출수기 차이는 나타나지 않았으나, 호밀의 피복구에서는 7일 정도 지연되었다. 피복작물에 대한 기장의 초장은 30%, 건물중은 70% 이상의 피해를 받았고, 조의 초장은 25%, 건물중은 60% 이상의 피해를 보였다. 그러나 시간이 경과함에 따라 조, 기장, 수수 모두 많은 회복을 보였으며, 특히 수수에서는 초장은 거의 회복되었으며, 건물중도 56% 까지 회복하였다.

• 인터넷정보학회논문지
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• 제12권5호
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• pp.171-179
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• 2011

본 연구는 디자인발상 과정에서 구글의 연관 검색어를 언어 자극으로 제공했을 경우 디자인 결과에 어떠한 결과를 주는지 그리고 그러한 과정에서 창의적 인지 과정이 어떻게 사용되는 지를 분석하였다. 디자인 발상 과정에 구글의 연관 검색어를 자극제로 5단계에 걸쳐 제공하였다. 구글 검색어는 다수의 사용자의 참여와 공헌에 의해 새롭게 재창조된 지식과 정보를 제공하는 집단지성에 기반을 두고 있다. 실험을 위해 두 가지 과제를 연관 검색어들과 함께 제공하였다. 디자인 발상실험 후 연관검색어의 사용여부와 빈도수 그리고 핀케의 12가지 제네플로어 모델이라는 3가지 기준에 의해 분석하였다. 본 연구의 결과를 요약하면 다음과 같다. 여러 단계의 연관 검색어들을 사용했으나, 초기 연관 검색어와 연관성이 높은 상위 단계의 검색어가 하위 단계의 검색어보다 더 많이 사용되었다. 또한 상위 단계와 하위 단계의 검색어들을 함께 사용했을 때 더 창의적의 결과가 나타났다. 핀케의 제네플로어 모델의 12가지 인지 과정에 따라 실험결과물을 분석한 결과 창의적 결과물은 단순히 연관 검색어를 사용하기 보다는 여러 단어들을 연합하고 변형하였으며 또한 창의적인 결과에는 12가지 인지 과정 중 개념적 해석, 기능적 추론과 맥락적 전이와 같은 인지 과정이 사용되었다.

• 생명과학회지
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• 제22권8호
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• pp.1107-1113
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• 2012

동양에서 한약 재료로 사용되는 칡(Pueraria thunbergiana)은 항산화, 항균효과 및 골다공증 치료 등의 다양한 효과가 있는 것으로 밝혀지고 있지만, 생태학적으로는 산림생태계를 파괴하는 종으로 알려져 있다. 본 연구는 칡의 면역학적 효과를 검증하여 유용한 자원으로 활용하는데 목적이 있다. 칡 추출물을 이용하여 생쥐 비장에 있는 면역세포 활성화 작용을 실험한 결과, 칡 추출물은 첫째, 농도 의존적으로 생쥐 비장세포의 증식 유도하였으며 IL-6, TNF-${\alpha}$, IL-2, IFN-${\gamma}$의 cytokine 생산을 증가시켰다. 둘째, 특히 비장세포 중 칡 추출물은 B세포를 자극하여 세포증식 및 IgM의 생산을 증가시켰다. 셋째, 대식세포주의 일산화질소 생산을 유도하였으며, 또 TNF-${\alpha}$, IL-6 및 IL-$1{\beta}$의 cytokine 분비를 유도하였다. 이상의 실험 결과, 본 실험에서 사용한 칡 추출물은 B세포와 대식세포 같은 면역세포의 증식과 각종 사이토카인을 생산을 유도하기 때문에, 면역반응을 조절하는 성분이 포함되어 있는 것으로 생각되며 특히, 아세톤 추출물이 물 추출물에 비하여 효과적이었다. 따라서 추가적인 실험을 통해, 면역반응을 조절하는 성분을 분리 정제하여 그 특성을 명확히 규명한다면, 각종 의약품이나 건강식품을 개발할 수 있는 원재료로서 칡을 이용할 수 있으며, 부수적으로 산림생태계에 복원에도 기여할 수 있을 것으로 사료된다.