• Bulletin of the Korean Chemical Society
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• 제34권2호
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• pp.489-494
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• 2013

An improved method for the preparation of monomethine cyanine dyes with quinoline nucleus by one-pot three-component using 1-methyl-2-quinolinethione, quaternized 2- or 4-methylheterocyclic compounds and methyl p-toluenesulfonate as starting materials was described. Compared with the traditional methods, the new synthetic method reduced the reaction steps, shortened the reaction time, avoided the separation and purification of the intermediate and reduced cost. The dyes absorbed in the region 478.0-563.0 nm and had molar extinction coefficients of $1.3{\times}10^4-9.4 {\times}10^4L\;mol^{-1}\;cm^{-1}$. Their fluorescence maxima and Stokes shifts were in the range of 525.2-594.4 nm and 16.2-80.6 nm in different solvents, respectively. From the spectral properties of the dyes in different solvents, it could be found that the ${\lambda}_{max}$ of the dyes were shorter in protonic solvents, and showed hypsochromic shifts with the increase of polarity of the solvents.

• Preventive Nutrition and Food Science
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• 제18권4호
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• pp.256-262
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• 2013

Four functional constituents, oxyresveratrol 3'-O-${\beta}$-D-glucoside (ORTG), oxyresveratrol (ORT), t-resveratrol (RT), and moracin (MC) were isolated from the ethanolic extract of mulberry (Morus alba L.) twigs by a series of isolation procedures, including solvent fractionation, and silica-gel, ODS-A, and Sephadex LH-20 column chromatographies. Their chemical structures were identified by NMR and FABMS spectral analysis. Quantitative changes of four phytochemicals in mulberry twigs were determined by HPLC according to cultivar, producing area, and heat processing. ORTG was a major abundant compound in the mulberry twigs, and its levels ranged from 23.7 to 105.5 mg% in six different mulberry cultivars. Three other compounds were present in trace amounts (<1 mg/100 g) or were not detected. Among mulberry cultivars examined, "Yongcheon" showed the highest level of ORTG, whereas "Somok" had the least ORTG content. Levels of four phytochemicals in the mulberry twigs harvested in early September were higher than those harvested in early July. Levels of ORTG and ORT in the "Cheongil" mulberry twigs produced in the Uljin area were higher than those produced in other areas. Generally, levels of ORTG and ORT in mulberry twigs decreased with heat processing, such as steaming, and microwaving except roasting, whereas those of RT and MC did not considerably vary according to heat processing. These results suggest that the roasted mulberry twigs may be useful as potential sources of functional ingredients and foods.

• Journal of Microbiology and Biotechnology
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• 제32권10호
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• pp.1315-1324
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• 2022

Fermentation is an effective process for providing various beneficial effects in functional beverages. Lactic acid bacteria and yeast fermentation-based biotransformation contribute to enhancement of nutritional value and digestibility, including lactose intolerance reduction and control of infections. In this study, the probiotic fermented fruit juice (PFJ) was produced by Lactobacillus plantarum TISTR 1465, Lactobacillus salivarius TISTR 1112, and Saccharomyces boulardii CNCM I-745 while mixed fruit juice (MFJ) was used as the basic medium for microorganism growth. The potential function, the anti-salmonella activity of PFJ, was found to be effective at 250 mg/ml of MIC and 500 mg/ml of MBC. Biofilm inhibition was performed using the PFJ samples and showed at least 70% reduction in cell attachment at the MIC concentration of Salmonella Typhi DMST 22842. The antioxidant activities of PFJ were determined and the results revealed that FSB.25 exhibited 78.40 ± 0.51 mM TE/ml by FRAP assay, while FPSB.25 exhibited 3.44 ± 0.10 mM TE/ml by DPPH assay. The volatile compounds of PFJ were characterized by GC-MS, which identified alcohol, aldehyde, acid, ester, ketone, phenol, and terpene. The most abundant organic acid and alcohol detected in PFJ were acetic acid and 2-phenylethanol, and the most represented terpene was β-damascenone. The sensory attributes showed scores higher than 7 on a 9-point hedonic scale for the FPB.25, illustrating that it was well accepted by panelists. Taken together, our results showed that PFJ could meet current consumer demand regarding natural and functional, fruit-based fermented beverages.

• 한국식품영양과학회지
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• 제39권8호
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• pp.1137-1142
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• 2010

함초발효액의 기능성 해명과 유용 기능성 성분 탐색연구의 일환으로 함초발효액으로부터 항산화 활성물질을 분리하여 구조해석 하였다. 함초발효액을 용매분획 하여 얻어진 용매분획물들 중 $CHCl_3$층이 높은 항산화 활성을 나타냈다. 그래서 $CHCl_3$층을 Sephadex LH-20 column chromatography의 분자체 효과 및 흡착능의 특성을 각각 이용하여 정제함으로써 2종의 항산화 활성물질을 분리하였다. 단리한 이들 화합물을 대상으로 MS 및 NMR 분석을 통하여 cirsiumaldehyde(1)와 chrysoeriol(2)로 각각 동정하였다. 이 화합물들은 $ABTS^+$ 및 DPPH radical-scavenging 활성을 나타냈으며, 화합물 2는 화합물 1에 비해 더 높은 활성을 나타냈다.

• 한국식품영양과학회지
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• 제35권6호
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• pp.713-720
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• 2006

식물자원으로부터 생리활성 물질의 이용측면에서 국내에서 자생하고 있는 광나무 잎을 대상으로 하여 다양한 생리활성을 가지는 것으로 보고된 페놀성 화합물의 분포를 확인하였다. 총 페놀성 함량은 $0.89{\sim}1.53%$, 축합형 탄닌은 $0.10{\sim}0.13%$였으며, flavonoid는 aglycone인 apigenin, luteolin과 칼럼크로마토그라피를 행하여 분획으로 분리한 후 가수분해하여 이들의 배당체를 확인하였다. 페놀산의 분획 중 유리형 페놀산 화합물에는 tyrosol, t-cinnamic acid, p-Hydroxybenzoic acid, vanillic acid, shikimic acid, protocatecuic acid등이 존재하였다. 에스테르형 페놀산 화합물에는 tyrosol, t-cinnamic acid, ferulic acid, esculetin, caffeic acid, p-coumaric acid, hydroxytyrosol 등이 함유되었다. 결합형 페놀성 화합물에는 tyrosol, t-cinnamic, p-coumaric acid와 미확인 페놀성 물질들이 존재하였다. 따라서 광나무 잎은 xanthine oxidase 활성저해, LDL 산화, 혈소판 응집저해, 항균활성 등 다양한 생리활성을 나타내는 것으로 보고되고 있는 tyrosol, hydroxytyrosol, protocatecuic acid 등 풍부한 페놀성 화합물을 함유하고 있으므로 유용한 식물자원이 될 수 있음을 확인하였다.

• 한국약용작물학회지
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• 제26권5호
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• pp.417-429
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• 2018

Background: The objective of this study was to make colloidal dispersions of the active compounds of radix of Angelica gigas Nakai that could be charaterized as nano-composites using hot melt extrusion (HME). Food grade hydrophilic polymer matrices were used to disperse these compound in aqueous media. Methods and Results: Extrudate solid formulations (ESFs) mediated by various HPMCs (hydroxypropyl methylcelluloses) and Na-Alg polymers made from ultrafine powder of the radix of Angelica gigas Nakai were developed through a physical crosslink method (HME) using an ionization agent (treatment with acetic acid) and different food grade polymers [HPMCs, such as HP55, CN40H, AN6 and sodium alignate (Na-Alg)]. X-ray powder diffraction (XRD) analysis confirmed the amorphization of crystal compounds in the HP55-mediated extrudate solid formulation (HP55-ESF). Differential scanning calorimetry (DSC) analysis indicated a lower enthalpy (${\Delta}H=10.62J/g$) of glass transition temperature (Tg) in the HP55-ESF than in the other formulations. Infrared fourier transform spectroscopy (FT-IR) revealed that new functional groups were produced in the HP55-ESF. The content of phenolic compounds, flavonoid (including decursin and decursinol angelate) content, and antioxidant activity increased by 5, 10, and 2 times in the HP55-ESF, respectively. The production of water soluble (61.5%) nano-sized (323 nm) particles was achieved in the HP55-ESF. Conclusions: Nano-composites were developed herein utilizing melt-extruded solid dispersion technology, including food grade polymer enhanced nano dispersion (< 500 nm) of active compounds from the radix of Angelica gigas Nakai with enhanced solubility and bioavailability. These nano-composites of the radix of Angelica gigas Nakai can be developed and marketed as products with high therapeutic performance.

• 생명과학회지
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• 제20권3호
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• pp.350-355
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• 2010

다양한 생리활성을 갖는 것으로 알려진 갈근을 중급 정도의 녹차와 혼합하여 갈근녹차를 제조하고 기호도와 관련이 있는 휘발성성분을 동시증류추출법에 의해 추출하여 GC 및 GC-MS로 분석, 동정하고 비교하였다. 갈근과 녹차를 1:1로 혼합한 갈근녹차의 향기성분으로 총 82종류를 동정하였다. 갈근녹차에는 갈근의 향기성분 18종류와 녹차의 향기성분 33종류 및 공통으로 포함된 31종류의 화합물들로 구성되어 있었다. 녹차의 향기성분으로 linalool, $\alpha$-ionone 및 nerolidol 등의 꽃향과 safranal, methyl butanal 등의 달콤한 향이 대부분을 차지하였다. 갈근의 향기성분으로 hexanal, trans-2-nonenal 및 $\beta$-selinene 등의 풋풋한 향과 decanal, limonene 등의 달콤한 향이 대부분을 차지하였다. 갈근녹차의 향기성분은 녹차의 향기성분인 달콤한 향을 띄는 furan류와 꽃향이나 산뜻한 과일 향을 띄는 linalool, nerolidol, 구수한 향을 띄는 pyrazine류와 pyrrole류에 갈근특유의 향기성분인 terpene계 화합물과 풋풋한 향을 띄는 trans-2-nonenal 및 $\beta$-selinene 등이 혼합되어 나타났다.

• 생명과학회지
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• 제19권8호
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• pp.1125-1131
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• 2009

진달래꽃을 60% ethanol로 추출하여 추출물의 H. pylori에 대한 저해활성의 측정과 저해물질의 분리 및 동정을 시행하였다. 또한 H. pylori균에 대한 항균활성은 60% ethanol 추출물에서 phenol 화합물을 200${\mu}g/ml$ 이상 주입하였을 때부터 22 mm이상의 clear zone이 관찰되어 H. pylori균에 대한 높은 항균력을 나타내었다. 저해물질의 분리는 Sephadex LH-20 column을 이용하여 분리한 결과 6개의 fraction으로 분리되었으며, H. pylori 저해활성을 나타낸 fraction D를 MCI-gel CHP-20 column을 이용하여 normal phase type인 ethanol 100%에서 0%로 농도를 감소시키며 용출한 결과 5개의 단일 물질 분획을 얻을 수 있었다. 그 중 세 가지의 물질에 대해 H. pylori저해활성이 나타나 FAB-MS, $^1H$와 $^{13}C$ NMR 및 IR spectrum을 사용하여 구조 동정한 결과 quercitrin (quercetin-3-O-rhamnopyranoside), myricitrin (myricetin-3-O-rhamnopyranoside) 및 quercetin인 것으로 확인되었다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 춘계학술발표회
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• pp.72-72
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• 2018

The root of Angelica gigas Nakai (AGN) is used as a traditional herbal medicine in Korea for the treatment of many diseases. However, a major challenge associated with the usage of the active compounds from AGN is their poor water solubility. Therefore, this work aimed to enhance the solubility of active compounds by a chemical (viz. surfactant) and physical (hot melt extrusion) crosslinking method (CPC). Infrared Fourier transform spectroscopy (FT-IR) revealed multiple peaks in extrudate solids representing new functional groups including carboxylic acid, alkynes and benzene derivatives. Differential scanning calorimetry (DSC) analysis of the extrudate showed lower glass transition temperature (Tg) and lower enthalpy (${\Delta}H$) (Tg: $43^{\circ}C$; ${\Delta}H$: <6 (J/g)) compared to the non-extrudate (Tg $68.5^{\circ}C$; ${\Delta}H$: 123.2) formulations. X-ray powder diffraction (XRD) analysis revealed amorphization of crystal materials in extrudate solid. In addition, nanonization, enhanced solubility and higher extraction of phenolic compounds were achieved in the extrudate solid. Among the different extrudates, acetic acid- and Span 80-mediated formulations showed superior extractions. We conclude that the CPC method successfully enhanced the production of amorphous nano dispersions from extrudate solid formulations.

• 대한화학회지
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• 제41권2호
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• pp.88-97
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• 1997

실릴트리플레이트$(Ph_{3-n}SiH(OTf)_n))$는 낮은 온도에서 트리펜일실란과 트리플산$(CF_3SO_3H)$의 반응에 의해 형성되며 이러한 화합물은 새로운 기능성 실란 유도체의 제조에 이용되고 있다. 실릴트리플레이트와 알캔일-, 알킨일마그네슘 브롬아이드 그리고 유기리티움 화합물과의 반응에 의해 새로운 실란 화합물 Ph_2SiHR(R=C{\equiv}(CPh,\;CH=CH_2,\;CH_2CH=CH_2,\;(CH_2)_2CH=CH_2,\;(CH_2)_3CH=CH_2)$을 높은 수율로 얻었다. 합성된 알켄일-, 알킨일실란은 백금촉매 하에서 카보실란 고분자$((Ph_2Si(CH_2)m)n;\;m=2∼4,\;n\le10)$5~6환고리 화합물을 형성하였다. 모든 생성물들은 NMR, UV, IR, 질량분석, 그리고 원소분석법에 의해 확인되었다.