• Title/Summary/Keyword: functional compounds

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Aptamers as Functional Nucleic Acids: in vitro Selection and Biotechnological Applications

  • You, Kyung-Man;Lee, Sang-Hyun;Aesul Im;Lee, Sun-Bok
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.8 no.2
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    • pp.64-75
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    • 2003
  • Aptamers are functional nucleic acids that can specially bind to proteins, peptides, amino acids. nucleotides, drugs, vitamins and other organic and inorganic compounds. The aptamers are identified from random DNA or RNA libraries by a SELEX (systematic evolution of ligands by exponential amplification) process. As aptamers have the advantage, and potential ability to be released from the limitations of antibodies, they are attractive to a wide range of therapeutic and diagnostic applications. Aptamers, with a high-affinity and specificity, could fulfil molecular the recognition needs of various fields in biotechnology. In this work, we reviewed some aptamer Selection techniques, properties, medical applications of their molecules and their biotechnological applications, such as ELONA (enzyme linked oligonucleotide assay), flow cytometry, biosensors, electrophoresis, chromatography and microarrays.

Decursin from the Rhizome of Belamcanda chinensis

  • Lee, Sang-Hyun;Ryu, Ji-Young;Son, Dong-Wook;Kim, Kyoung-Soon;Lee, Sang-Chul;Kim, Bak-Kwang
    • Natural Product Sciences
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    • v.10 no.2
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    • pp.89-91
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    • 2004
  • Six components were isolated from the $CH_2Cl_2$ fraction of Belamcanda chinensis rhizome by open column chromatography. Their structures were elucidated as ${\beta}-sitosterol$ (1), apocynin (2), decursin (3), iristectorigenin A (4), irigenin (5) and tectorigenin (6) by spectral analysis. Among these compounds, decursin (3) was isolated for the first time from a plant of the family Iridaceae.

Electronic and Magnetic Structures of {Ca,Sr,Ba}$Fe_2As_2$ : Dynamical Mean Field Theory Approach ({Ca,Sr,Ba}$Fe_2As_2$의 전자 및 자성 구조: 동력학적 평균장 이론 접근)

  • Lee, Geun-Sik;Shim, Ji-Hoon
    • Progress in Superconductivity
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    • v.13 no.2
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    • pp.85-89
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    • 2011
  • Using the density functional theory and its combination to the dynamical mean field theory (DMFT), we have studied the electronic and magnetic structures of Fe-based superconductors, $AFe_2As_2$ (A=Ca, Sr, Ba). Our results for the electronic structure agree well with existing angle resolved photoemission spectroscopy (ARPES) data. The temperature dependent magnetization has been calculated using DMFT, and the magnetic transition temperatures are reasonably consistent with the experimentally observed trend for three compounds.

Bioactive Components in Milk (우유의 생리활성 물질)

  • Kim, Geun-Bae
    • Journal of Dairy Science and Biotechnology
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    • v.28 no.1
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    • pp.43-52
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    • 2010
  • In addition to the nutritional values, milk has a wide range of bioactive compounds which have been found to be increasingly important for physiological and biochemical functions on human metabolism and health. Bioactive components in milk comprise specific proteins, peptides, lipids and carbohydrates. Especially, milk proteins are known to exert a wide range of nutritional, functional, and biological activities. And milk proteins are considered the most important source of bioactive peptides, including antihypertensive, antithrombotic, antimicrobial, antioxidative, immunomodulatory, and opioid peptides. Many ingredients containing specific bioactive peptides derived from milk protein hydrolysates have been launched on the market and are currently under development. In future studies more emphasis should be given to the health-promoting effect in the well-defined human clinical studies for the successful development of function foods based on the milk-derived bioactive components.

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Volatiles of Chrysanthemum zawadskii var. latilobum K.

  • Chang, Kyung-Mi;Kim, Gun-Hee
    • Preventive Nutrition and Food Science
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    • v.17 no.3
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    • pp.234-238
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    • 2012
  • The volatile aroma constituents of Chrysanthemum zawadskii var. latilobum K. were separated by hydro distillation extraction (HDE) method using a Clevenger-type apparatus, and analyzed by gas chromatography-mass spectrometry (GC/MS). The yield of C. zawadskii var. latilobum K. flower essential oil (FEO) was 0.12% (w/w) and the color was light green. Fifty-five volatile chemical components, which make up 88.38% of the total aroma composition, were tentatively characterized. C. zawadskii var. latilobum K. FEOs contained 27 hydrocarbons, 12 alcohols, 7 ketones, 4 esters, 1 aldehyde, 1 amine, and 3 miscellaneous components. The major functional groups were terpene alcohol and ketone. Borneol (12.96), (${\pm}$)-7-epi-amiteol (12.60), and camphor (10.54%) were the predominant volatiles. These compounds can be used in food and pharmaceutical industries due to their active bio-functional properties.

Effect of Reduction of Phytate and Phenol Compound on the Functional Properties of Sesame Protein Concetrate (참깨박에 함유된 Phytate와 Phenol 화합물의 제거가 단백질의 기능성에 미치는 영향)

  • Kim, Jin;Park, Jyung-Rewng
    • Journal of the East Asian Society of Dietary Life
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    • v.3 no.2
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    • pp.129-137
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    • 1993
  • This study was attempted to determine the effect of reduction of phytate and phenol compound on the functional properties of sesame protein concentrate. The concentrates were prepared by using dist-water, HCI and butanol. The content of phytate and phenol compound in defatted sesame meal were 4.55% and 3.42% respectively. Considerable amount of phytate was reduced by using HCI, and butanol was effective in removing phenol compounds, Higher bulk density and fat absorption were found in sesame protein concentrate prepared by butanol but higher water absorption was found in the concentrate prepared by dist-water. Also, emulsifying and foaming properties were improved by butanol treatment.

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Identification of Ceftiofur Oxidation Products by High-Performance Liquid Chromatography/Electrospray Ionization/Tandem Mass Spectrometry

  • Lim, Young-Hee;Park, Deok-Hie;Youn, Yeu-Young;Kim, Kyung-Hoon;Cho, Hye-Sung
    • Mass Spectrometry Letters
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    • v.2 no.1
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    • pp.16-19
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    • 2011
  • Oxidation products of ceftiofur were formed in hydrogen peroxide solution. The structures of the ceftiofur oxidation products were characterized by high-performance liquid chromatography/electrospray ionization/tandem mass spectrometry (HPLC/ESI/MS/MS). The products were identified as compounds oxidized at the sulfur of a cephem ring. For further analysis, experiments were performed using $O^{18}$-labeled hydrogen peroxide. In addition, density-functional calculations were carried out for six possible oxidation products to support the experimental results.

Theoretical study on electronic properties of deoxyfluorinating sulfur-based reagents

  • Lim, Soobin;Lee, Eunsung
    • Journal of Radiopharmaceuticals and Molecular Probes
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    • v.2 no.1
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    • pp.51-55
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    • 2016
  • Organofluorine compounds have become increasingly important as pharmaceuticals, radiopharmaceuticals, agrochemicals, and material science. Recent advances on the efficient introduction of fluorine to organic molecules are mainly results of development of transition metal catalysts and fluorination reagents. Among the various fluorination reagents, we have been interested in developing more efficient sulfur-based deoxyfluorinating reagents. Here we report various electronic properties of five popular sulfur-based deoxyfluorinating reagents using density functional theory calculation. We believe that the theoretical study on the reagents will assist the rational design of new deoxyfluorinating reagents.

Overview of active packaging to maintain the quality of fresh food products - focusing on controlled release packaging (식품의 선도 유지를 위한 액티브 포장 연구 고찰 기능성 방출 조절 포장 중심)

  • Lee, Myung-Ho;Lee, Youn Suk
    • Food Science and Industry
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    • v.50 no.2
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    • pp.27-36
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    • 2017
  • Today, the food packaging industry has a great interest in using active packaging to fresh food product as a solution for the future to positively provide its quality, safety and shelf life. Many researches have extensively studied functional packaging strategies in recently years. Controlled release packaging (CRP) is an innovative packaging technology in the packaging polymer matrix from which can active agents are delivered in a controlled way into the product. CRP technology is well-suited for controlling release of antimicrobial compounds and antioxidants to prevent food degradation reactions such as microbial growth and lipid oxidation. Advances in CRP technology allow food packaging manufacturers to challenge the development of better functional food packaging systems. This overview examines the most recent developments and technologies of active packaging for applying the food industry. The scope of this article has mainly been focused on controlled releasing systems.

Research and Application of Nano-particles as Oil Additives

  • Xue, Qunji;Liu, Weimin;Zhang, Zhijun
    • Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
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    • 2002.10b
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    • pp.265-266
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    • 2002
  • Nano materials have great potential for development of advanced lubricating and protecting materials. Nano-particles capped by organic compound such as organic acid, dialkyldithiophosphate (DDP) are capable to disperse stably in lubricating oils, and are able to reduce wear and to increase load-carrying capacity.

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