• 제목/요약/키워드: fucose

검색결과 154건 처리시간 0.026초

전통 침채류 유래 다당 생산균의 분리 특성 (Isolation and Characterization of Exopolysaccharide-Producing Bacteria from Korean Fermented Vegetables)

  • 권태연;심상민;허민영;안두현;신광순;이종훈
    • 한국미생물·생명공학회지
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    • 제35권3호
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    • pp.191-195
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    • 2007
  • 탄닌 분해균 선발배지를 이용하여 탄닌 분해활성 보유 유산균을 발효 침채류로부터 분리하는 과정에서 오이소박이, 파김치, 총각김치로부터 다당류로 추정되는 점질물질을 생산하는 균주 4종을 선발하였다. 이들 균주의 165 rDNA 염기서열에 의한 계통유전학적 동정 결과, CK31은 Enterobacter cowanii, CK32는 Escherichia senegalensis, YK5는 Enterobacter asburiae, GK23은 Enterobacter ludwigii와 99% 이상의 매우 높은 상동성을 나타내었다. 가장 많은 양의 점질 물질을 세포 외로 생성하는 CK3l 균주는 rhamnose, fucose, arabinose, mannose, galactose, glucose의 중성당 조성을 가지고 있는 분자량 약 420 kDa heteropolysaccharide를 생산하는 것으로 나타났다.

Antithrombin Active Polysaccharide Isolated from the Alkaline Extract of Red Ginseng

  • Kim Dong Chung;In Man-Jin;Lee Ji Young;Hwang Yoon Kyung;Lee Sung Dong
    • Journal of Ginseng Research
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    • 제23권4호
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    • pp.217-221
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    • 1999
  • 홍삼으로부터 염기성 수웅액 추출, 음이온교환 크로마토그래피 및 겔여과 크로마토그래피를 순차적으로 수행하여 항트롬빈 활성을 보이는 다당체를 분리하였다. 이 다당체는 셀룰로스 아세테이트막을 사용한 전기영동에서 하나의 띠로 나타나 비교적 순수하게 정제되었음을 알 수 있었고, 겔여과 크로마토그래피에서 결정된 평균 분자량은 약 177kDa이었다. $40.2\%$의 유론산, $9.2\%$의 황산기 및 $1.5\%$의 단백질을 포함하는 산성다당체였고, 구성 중성당으로 rhamnose, mannose, galactose, arabinose, glucose, fucose, xylose를 1.00 : 0.88 :0.86 : 0.78 : 0.70 : 0.33 : 0.22의 비율로 포함하고 있었다. 이 다당체는 트롬빈에 의한 피브리노겐의 응고를 농도에 비례하여 저해하였고, 혈장을 사용한 실험에서 내인성 경로를 통해 혈액응고를 저해하는 것으로 나타났다.

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능이자실체의 Glycoprotein (Glycoprotein in the Fruit Body of Sarcodon aspratus)

  • 조남석;최태호;조희연;안드레 레오노비치
    • Journal of the Korean Wood Science and Technology
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    • 제32권5호
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    • pp.51-58
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    • 2004
  • 본 연구에서는 능이가 포함하고 있는 각종 생리활성 성분올 분석하고, 열수추출-에틸알코올 침전에 의하여 분리한 glycoprotein 의 특성을 구명코자 하였다. 능이(Sarcodon aspratus)의 무기성분 및 아미노산의 조성을 분석하였는 바, 능이는 Ca, Mg, Zn, Mn, Fe, Cu 및 Ph를 함유하였으며, 특히 Ca 및 Na 성분이 많이 함유되어 있었다. 유리 및 총 아미노산을 분석한 결과, 14종의 유리아미노산이 검출되었고. glutamic acid, alanine, arginine 등이 많았다. 총아미노산은 15종이었으며, glutamic acid가 가장 많았고, aspartic acid, serine, threonine 등이 그 다음이었다. 열수-95% 에틸알코올 추출에 의하여 70.6%의 당과 3.32%의 glycoprotein을 얻었으며, 에틸알코올의 농도에 따라 당의 함량이 상이한 glycoprotein을 얻을 수 있었는데, 에틸알코올의 농도가 30~70%로 낮은 경우, 당함량이 92% 이상으로 매우 높았다. 조 glycoprotein (GP). 분자량 30만 이상의 분획(P), P분획 가운데 DEAE-Sephadex에 흡착되지 않는 분획(P-1), P-1을 다시 Sepharose 2B로 젤여과하여 얻은 부분(P-2), DEAE-Sephadex에 흡착되는 부분 (P-3) 등으로 분획한 결과, 정제과정에서 total sugar의 함량은 점차 증가하였으며, 단백질의 함량은 점차 감소되는 것으로 나타났다. 단당류의 조성을 분석한 결과, GP 빛 P-3 에서 glucose, galactose, mannose, fucose 등 4종의 당이 검출 되었으며, GP에는 glucose 가 거의 대부분을 이루고 있었고, glutamic acid, serine, alanine, glycine 등이 고농도로 함유되어 있었다. P-3에는 mannose 및 aspartic acid, glutamic acid, glycine 등의 아미노산이 함유되어 있었다. P-2에서는 glucose가 많았고, 다른 fraction에는 없었던 fucose의 함량이 높았으며 mannose는 검출되지 않았다. 한편 아미노산조성은 조 glycoprotein (GP)에는 glutamic acid, serine, alanine, glycine 등이 고농도로 함유되었으며, P-3분획에서는 aspartic acid, glutamic acid, glycine 등이 함유되어 있었다. 한편 P-2 fraction의 경우는 단백질함량이 1.1%로서 매우 낮았으며, 분자량은 700,000 이상이었다. aspartic acid, glutamic acid, alanine이 비교적 다량 함유되었으며, mannose 및 cysteine은 거의 확인되지 않았다.

콩나물 생장중 세포벽 비섬유성 중성당의 변화 (Changes of Non-Cellulosic Neutral Sugars of Cell Wall in Soybean Sprouts)

  • 신승렬;박찬성;김주남;김광수
    • 한국식품영양과학회지
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    • 제27권6호
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    • pp.1041-1046
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    • 1998
  • This study was carried out to investigate the changes and composition of the non-cellulosic neutral sugars in cell wall of soybean sprouts during growth. The composition of non-cellulosic neutral sugars in cell of soybean sprouts was rhamnose, fucose, xylose, arabinose, mannose, galactose and glucose. The galactose content of cell wall was higher than other non-cellulosic neutral sugars, and was remarkably decreased during growth. The major non-cellulosic sugars of pectic substances were rhamnose, arabinose, and galactose. The arabinose content of pectic substance was increased in cotyledon and hypocotyl during growth. The contents of non-cellulosic neutral sugars were decreased in hypocotyl during growth. The galactose content of pectic substance was higher in cotyledon than those in hypocotyl, and was increased in cotyledon. The content of rhamnose was higher in ionically associated pectic substance than that in covalently bounded pectic substance. The major non-cellulosic neutral sugars of hemicellulose were glucose, rhamnose, arabinose and galactose. The galactose of hemicellulose was decreased remarkably during growth.

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Human milk oligosaccharides: the novel modulator of intestinal microbiota

  • Jeong, Kyung-Hun;Nguyen, Vi;Kim, Jae-Han
    • BMB Reports
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    • 제45권8호
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    • pp.433-441
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    • 2012
  • Human milk, which nourishes the early infants, is a source of bioactive components for the infant growth, development and commensal formulation as well. Human milk oligosaccharide is a group of complex and diverse glycans that is apparently not absorbed in human gastrointestinal tract. Although most mammalian milk contains oligosaccharides, oligosaccharides in human milk exhibit unique features in terms of their types, amounts, sizes, and functionalities. In addition to the prevention of infectious bacteria and the development of early immune system, human milk oligosaccharides are able to facilitate the healthy intestinal microbiota. Bifidobacterial intestinal microbiota appears to be established by the unilateral interaction between milk oligosaccharides, human intestinal activity and commensals. Digestibility, membrane transportation and catabolic activity by bacteria and intestinal epithelial cells, all of which are linked to the structural of human milk oligosaccharides, are crucial in determining intestinal microbiota.

인체 타액내 당단백질의 구조와 기능 (Structure and Function of Glycoproteins in Human Saliva)

  • Song Han
    • Journal of Oral Medicine and Pain
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    • 제20권1호
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    • pp.19-28
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    • 1995
  • 타액은 구강 환경을 조절하는 여러 가지 유기질과 무기질의 혼합물로 구성되어 있다. 구강 점막은 여러 타액 점액 단백질과 타액 항세균 단백질에 의해서 윤활이 되며 보호된다.타액의 다른 작용은 구강 점막을 축축하게 하고 음식을 부드럽게 한다. 구강건조증은 세균의 침착을 야기시키거나 점막면을 거칠게 하여 출혈이 되기 쉽게 하며 이로 인해 감염이 야기될 수 있다. 이러한 타액의 보호 작용은 mucin, fibronectins, proline-rich glycoproteins, histidine-rich proteins, $\alpha$-amylase, s-IgA 같은 특별한 타액 당단백질에 기인한다고 하는 것이 지난 30년 동안에 알려져 있다. 이러한 분자들의 구조, 구조와 기능사이의 관계, 타액 내 이러한 물질들의 농도에 관한 것들이 알려지고 있는 중이다. 이러한 타액 당단백질 특히 mucin, fibronectin, fucose-rich protein과 s-IgA의 구조와 기능에 대한 현재의 견해들을 이 논문에서 요약하고자 한다.

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Characterization of Oilgosaccharide Moieties of Rat Intestinal Phytase

  • Yang, Won-Jin;Kim, Kil-Woong
    • Archives of Pharmacal Research
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    • 제17권5호
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    • pp.309-313
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    • 1994
  • Phytase of rat intestine had a large amount of oilgosacchanrides ; The enzyme consisted of two different subunits with the molecular weights of 90 KDa and 70 KDa in its intack form, whereas the apparent molecular weights tumed to 72 KDa and 52 KDa, respectively, after deglycosylation. The treatment with glycopeptidase F reduced the molecular weights from 90 KDa and 70 KDa to 83 KDa and 52 KDa, respectively, While endoglycosidase H caused no change in their molecular weights. These results indicate that the 70KDa subunit contains only the N-linked oilgosaccharide chains, while the 90KDa subunit ocntains O-linked oilgosaccharides as well as N-linked ones. Enzyme-linked lectin assays suggeted that bisecting N-acetyl-D-glucosamine and galactose 1-4 N-acetyl-D-glucosamine structures were present and that fucose was included in these oilgosaccharide moieties. Sialic acid was not found in either subunit.

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Compositional Sugar Analysis of Antitumor Polysaccharidees by High Performance Liquid Chromatography and Gas Chromatography

  • Kim, Yeong-Shik;Park, Kyung-Shin;Park, Ho-Koon;Kim, Sung-Whan
    • Archives of Pharmacal Research
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    • 제17권5호
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    • pp.337-342
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    • 1994
  • Carbohydrate analysis is important in studying structure and activity of complex polysaccharides. New analytical method was applied to get an information on the composition of polysaccharides showing antitumor activity. Monosaccharides were labeled with 7-amino-1, 3-naph-thalenedisulfonic acid (7-AGA) by reductive amination and separated by HPLC. Five kinds of polysaccharides from Basidiomycetes were hydrolyzed and analyzed in combination with electrophresis and HPLC. At the same time, alditol acetate derivatives were prepared and analyzed by gas chromatography. Two different techniques using different derivatization methods showed very similar results. The monosaccharides from Coriolus versicolor and Cordyceps militaris were glucose and galactose. Phellinus linteus composed of glucose, glactose, mannose, arabinose and fucose. The HPLC method with fluorescence detector was very sensitive compared to other methods.

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Purification and Properties of Non-Cariogenicity Sugar Produced by Alkalophilic Bacillus sp. S-1013

  • Ryu, Il-Hwan;Kim, Sun-Sook;Lee, Kap-Sang
    • Journal of Microbiology and Biotechnology
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    • 제14권4호
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    • pp.751-758
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    • 2004
  • The NCS(Non-Cariogenicity Sugar) from Bacillus sp. S-1013 was purified by cold acetone and methanol precipitation, and DEAE-cellulose ion-exchange and Sephadex G-100 column chromatographies, to yield an amorphous yellow syrup. The melting point and $[\alpha]_D^{20}$ were 155-$157^{\circ}C$ and +53, respectively. Instrumental analyses such as FT-IR, $^1H-NMR, and ^{13}C-NMR$ showed that the NCS contained an O-H group, C-H, C=O, $NH_2$, anomeric carbon, anomeric proton, N-acetylgalactose, fucose, and neuramic acid, thus, the NCS was determined to be a trisaccharide of Fuc($1\longrightarrow4$)GalNAc($2\longrightarrow6$) NeuAc.

Lectin-binding properties of chicken primordial germ cells during embryonic development

  • Kim, Duk-Kyung;Seo, Sam-Youl;Lee, Eun-Young;Lee, Seul-Ki;Han, Jae-Yong
    • 한국가금학회:학술대회논문집
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    • 한국가금학회 2001년도 제18차 정기총회 및 학술발표 PROCEEDINGS
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    • pp.69-70
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    • 2001
  • Lectins have great potential as to determine the alternation of the distribution of cell surface carbohydrates during cellular development and differentiation. Here, we investigated the presence and distribution of cell surface carbohydrates on chicken primordial germ cells (PGCs) during the migration and gonadal stages using a variety of lectins. A total of six FITC-labelled lectins from several specificity classes were used: ConA (glucose/mannose), WGA (N-acetylglucosamine), STA (N-acetylglucosamine), DBA (N-acetylgalactosamine/galactose), UEA-I (fucose) and PHA-E (oilgosaccharide). As a results, PGC-specific binding was observed in STA. PGCs of migration stage (2.5- and 5.5-day embyos) were STA-positive whereas PGCs of 10-day embryonic gonad were not. The results suggest that N-acetylglucosamine residuse are present specifically in migrating chicken PGCs and changes during development.

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