• Title/Summary/Keyword: fruits protease

Search Result 16, Processing Time 0.024 seconds

Characteristics of Crude Protease from Fruits and Traditional Korean Fermentation Starters (과일과 전통발효제로부터 추출한 단백질분해 조효소의 비교 특성)

  • Yoo, Seon-A;Seo, Seung-Ho;Hyun, Seo-Yeon;Son, Hong-Seok
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.42 no.9
    • /
    • pp.1461-1466
    • /
    • 2013
  • In this study, we ascertained the characteristics of crude protease extracted from fruits (fig, kiwifruit and pineapple) as well as traditional Korean fermentation starters (bio nuruk, traditional nuruk, meju and rice koji) to determine their suitability for industrial application. Crude protease extracted from traditional Korean fermentation starters was found to have a higher optimum temperature ($70^{\circ}C$) and salt concentration (1~3%) but a lower optimum pH (3~6) value compared to the corresponding values for the protease extracted from fruits. On comparison, the total activities of protease per gram unit follow the order: bio nuruk> rice koji> traditional nuruk> fig> pineapple> meju> kiwifruit. Based on our results, we conclude that protease extracted from traditional Korean fermentation starters has potential for application in food industry, for example, as a meat tenderizer for sausage manufacturing and as a protease for cheese production.

Proteolytic Effect of Fruit Flesh and Crude Enzyme Extract from Fruits on Myofibrilar Protein (과실유래 단백질 조효소액과 과육의 근원섬유 분해 효과에 관한 연구)

  • Kim, Mi-Hyun;Rho, Jeong-Hae;Kim, Mee-Jeong
    • Korean journal of food and cookery science
    • /
    • v.26 no.3
    • /
    • pp.323-329
    • /
    • 2010
  • Studies on the tenderizing effect of fruits has been limited even though fig, kiwifruit, pear, and pineapple cultivated in Korea are utilized commonly during cooking for their proteolytic properties. Therefore, the characteristics of these fruits were investigated by treating beef with their crude protease extracts. The protease effects of crude protease extract from the fruits on casein and myofibrilar protein were in the following order : pineapple > kiwifruit > fig > pear. Electrophoretic analysis results found that pineapple, kiwifruit, and fig cleaved myosin heavy chain into smaller fragments. The myofibrilar fragmentation ratio of crude protease extracts was the highest for pineapple whileas the lowest for pear. Ground fruits (5% and 10%) increased amounts of soluble nitrogen and decreased shear force of beef. Pineapple was the most effective while pear was the least effective. Decrease in springiness and gumminess was observed by texture profile analysis of beef treated with fruits, especially pineapple and kiwifruit. Among the 5% treatments, pineapple and kiwifruit produced the highest tenderness. Additionally, 10% treatment was less preferable than the 5% treatment.

Protease activity and meat-tenderizing effect of Hypsizygus marmoreus (느티만가닥버섯의 단백질분해효소 활성과 연육증진효과)

  • Shin, Bok-Eum;Baek, Il-Sun;Kim, Jeong-Han;Lee, Yun-Hae
    • Journal of Mushroom
    • /
    • v.17 no.4
    • /
    • pp.235-240
    • /
    • 2019
  • In this study, we investigated the effects of pH, temperature, and salt concentration on protease activities in Hypsizygus marmoreus, and the ability of H. marmoreus extract to tenderize beef. In fact, H. marmoreus was shown to have higher protease activity than kiwi fruits. The pH of beef was dose dependently increased with addition of H. marmoreus extract. Cooking loss, cutting strength, and color value (L, a, b) were also dose dependently decreased with addition of H. marmoreus extract. In sensory assessments, H. marmoreus extract dose dependently enhanced perception of tenderness, flavor, and taste. In contrary, use of kiwi fruits as a meat tenderizer decreased positive perception of taste. The protease activitiy of H. marmoreus decreased sharply at pH < 2.0, and temperature > 50 ℃. Protease activity was relatively stable in NaCl concentrations ranging from 0-5 M, but incrementally decreased with increasing NaCl. These results suggest that, H. marmoreus extract can improve the texture and taste of beef.

Quality of Fresh Vegetable and Fruit Juice produced with Low-Speed and High-Speed Juicers (저속 압착 방식의 착즙기와 고속 파쇄 방식의 블랜더를 사용한 주스의 품질 변화에 대한 연구)

  • Kim, Youngsung
    • The Korean Journal of Food And Nutrition
    • /
    • v.30 no.3
    • /
    • pp.568-577
    • /
    • 2017
  • Vegetables and fruits contain a great deal of vitamins, minerals, dietary fiber and phytochemicals. Therefore, healthconcious consumer prefer beverage made from fresh fruits and vegetables due to their health benefits. This study was conducted to investigate differences in nutritional composition and sensory characteristics of juices depending on the apparatus used: either a low-speed juicer or high-speed blender. All ingredients could be made into juice without addition of water using the low speed juicer. However, addition of water was necessary to produce juice with the high-speed blender. Phenol and flavonoid content, were higher in juice made with the low-speed juicer than that produced with the high-speed blender and were correlated with DPPH radical scavenging ability. Protease activity of pineapples juices was not significantly different for the two methods, but protease activity of kiwi juice was about 8 times higher in juice made with low-speed juicer than that made with the high-speed blender. SOD activity also tended to be higher in the juice made with low-speed juicer. The concentration of dissolved oxygen in the juice made with high-speed blender was higher than that of juice made with a low-speed juicer and was correlated with color change. In addition, the high-speed blender caused an increase juice temperature, but temperature was not changed during use of the low-speed juicer. The noise level of the low-speed juicer was low, but the high-speed blender had high noise intensity comparable to that of railway or aircraft noise. In the sensory evaluation of juice, juice made with the low-speed juicer was preferred over juice made with the high-speed blender. As a result, the overall quality of the juice produced using the low-speed juicer was superior.

Quality Changes of Fresh Vegetable and Fruit Juice by Various Juicers (주스제조 장치에 따른 채소 및 과일 주스의 품질 변화)

  • Choi, Moon-Hee;Kim, Min-Joo;Jeon, Young-Jin;Shin, Hyun-Jae
    • KSBB Journal
    • /
    • v.29 no.3
    • /
    • pp.145-154
    • /
    • 2014
  • A fresh vegetable and fruit juice has become a new healthy food available for detoxification, dieting and health. This paper presents the useful information about the quality changes of fresh juice according to different juicer. Quality of fresh juice could be evaluated by several factors such as juice yield, enzyme activity, antioxidant activity, polyphenol contents, and anti-inflammatory activity. The juice yields of 12 different vegetables and fruits were compared using 6 different juicers and it was observed that the yield of slow juicer was better than that of conventional blender. Among 12 samples, the juice yield of grape is the best and the pH of the juice was in the acidic range of 3 and 4. Kiwi and grapefruit were the best in terms of protease enzyme activities by Hemoglobin units on the tyrosine basis and Spectrophotometric acid protease unit and papain units on the tyrosine basis of KFDA protocols. The total polyphenol contents were also high in kiwi and grapefruit. The antioxidant activity by diphenyl-1-picrylhydrazyl (DPPH), superoxide dismutase (SOD), and radical scavenging assay were high in the order of kiwi, grapefruit, grape, tomato, and orange. Anti-inflammatory activities were also assay for 12 samples with 6 juicers. It can be concluded that of fresh fruit and vegetable juice provides a source of antioxidant components and enzymes with high activity. And the enzyme activities could be used as one of the quality indicator of fresh juice. Concerning the juicers used in this study, slow juicer could be recommended to prepare the fresh juice in terms of the juice quality.

Antagonistic Activities of Bacillus spp. Strains Isolated from Tidal Flat Sediment Towards Anthracnose Pathogens Colletotrichum acutatum and C. gloeosporioides in South Korea

  • Han, Joon-Hee;Shim, Hongsik;Shin, Jong-Hwan;Kim, Kyoung Su
    • The Plant Pathology Journal
    • /
    • v.31 no.2
    • /
    • pp.165-175
    • /
    • 2015
  • Anthracnose is a fungal disease caused by Colletotrichum species that is detrimental to numerous plant species. Anthracnose control with fungicides has both human health and environmental safety implications. Despite increasing public concerns, fungicide use will continue in the absence of viable alternatives. There have been relatively less efforts to search antagonistic bacteria from mudflats harboring microbial diversity. A total of 420 bacterial strains were isolated from mudflats near the western sea of South Korea. Five bacterial strains, LB01, LB14, HM03, HM17, and LB15, were characterized as having antifungal properties in the presence of C. acutatum and C. gloeosporioides. The three Bacillus atrophaeus strains, LB14, HM03, and HM17, produced large quantities of chitinase and protease enzymes, whereas the B. amyloliquefaciens strain LB01 produced protease and cellulase enzymes. Two important antagonistic traits, siderophore production and solubilization of insoluble phosphate, were observed in the three B. atrophaeus strains. Analyses of disease suppression revealed that LB14 was most effective for suppressing the incidence of anthracnose symptoms on pepper fruits. LB14 produced antagonistic compounds and suppressed conidial germination of C. acutatum and C. gloeosporioides. The results from the present study will provide a basis for developing a reliable alternative to fungicides for anthracnose control.

Antioxidant Activity Evaluation of Vegetable Complex Extract of Pre-Heat Treatment Process (사전 열처리 공정 처리한 야채류 복합추출물의 항산화활성 평가)

  • Kim, Hyun-Kyoung;Hwang, Hye-young;Kim, Heung-Tae;Kim, Hye-Jin;Seo, Soo-Jin
    • The Journal of the Convergence on Culture Technology
    • /
    • v.8 no.2
    • /
    • pp.409-416
    • /
    • 2022
  • As a food ingredient, the extract has been proven of its antioxidant properties and safe to be consumed. It's increased antioxidant activity was evaluated by comparing its activity to the complex extract of fruits and vegetables, which is known to exhibit both antioxidant and physiological activities. In this experiment, after pre-heat treatment process, the increased antioxidant extract showed 41% of DPPH radical scavenging activity. The remarkable antioxidant activity of the produced extract will become useful in creating cosmetic or health products.

The Properties of Proteolytic Enzymes in Fruits (Pear, Kiwifruit, Fig, Pineapple and Papaya) (배, 키위, 무화과, 파인애플, 파파야에 존재하는 단백질 분해효소의 특성 비교)

  • 배영희;노정해
    • Korean journal of food and cookery science
    • /
    • v.16 no.4
    • /
    • pp.363-366
    • /
    • 2000
  • This study was attempted to compare the properties of proteolytic enzymes in fruits(Pear, Kiwifruit, Fig, Pineapple and Papaya) for the application of scientific information to cooking. The results were as follows: 1. The optimum temperature of crude proteolytic enzymes in pear, fig and pineapple is 60$^{\circ}C$ and it was relatively active in 40-70$^{\circ}C$; papaya showed max. activity in 60$^{\circ}C$ and highly stable activity in 40-80$^{\circ}C$, but kiwifruit showed max. activity in 40$^{\circ}C$ and it maintained to 70$^{\circ}C$. 2.. The crude proteolytic enzymes of pear, fig, pineapple and papaya showed opt. pH at pH 7.0 and maintained at pH 5.0-8.0, but max. activity of kiwifruit observed in pH 3.0 and pH 5.0-8.0. 3. As a result of comparison of total activities of fruits per kg unit, the order of activities was pineapple > kiwifruit > papaya > fig > pear.

  • PDF

Application of Proteolytic Enzymes in Fruits for Meat Tenderization (과일에 존재하는 단백질 분해효소의 식육연화효과에 관한 연구)

  • 배영희;노정해
    • Korean journal of food and cookery science
    • /
    • v.16 no.4
    • /
    • pp.367-371
    • /
    • 2000
  • In order to study the tenderizing effect of proteolytic enzymes in fruits, beef(M. semimembranosus) was marinated with meat sauce containing each fruit juices. After cooking, the shear force was measured by Rheometer and evaluated the sensory properties of beef by quantitative descriptive analysis method. The results are as follows: 1. The combination ratio of meat sauce:water was 2:1 with pH 5.0∼5.5 showed the max. tenderness. 2. As a result of shear force test, the decrease of shear force was pineapple>papaya>fig>kiwifruit>pear: especially, pineapple, papaya and fig tendered the beef significantly comparing with pear and kiwifruit at p<0.001. 3. The tendering effect of pineapple and papaya on the meat showed significant difference (p<0.01) comparing with pear in tenderness and overall acceptability by sensory evaluation; and there was a significant difference between pear and papaya in taste (p<0.05). 4. There was highly significant correlation between mechanical tenderness and sensory properties: correlation of fruit and mechanical tenderness was -.877(p<0.01); between mechanical tenderness and overall acceptability, r = .532(p<0.01); between fruit and sensory tenderness, r = .495(p<0.01); between mechanical tenderness and sensory tenderness, r = .490(p<0.01). At p<0.05, between taste and juiciness, r = .208.

  • PDF