• 제목/요약/키워드: fruit stem

검색결과 394건 처리시간 0.028초

파인애플 bromelain의 정제 및 특성 (Purification and characterization of bromelain isolated from pineapple)

  • 서형주;이호;조홍연;양한철
    • Applied Biological Chemistry
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    • 제35권4호
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    • pp.300-307
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    • 1992
  • 파인애플의 과육과 줄기로부터 fruit-bromelain을 정제도 18배와 수율 23%, stem-bromelain을 정제도 46배와 수율 23%로 각각 전기영동적으로 균일하게 정제하였다. 두 효소의 분자량을 Sephadex G-200에 의해 측정한 결과, fruit-와 stem-bromelain의 분자량은 32.5 KDa와 37 KDa이었으며, 이들은 공히 1개의 subunit로 구성된 효소단백질이었다. Fruit-bromelain은 pH 8.0과 $70^{\circ}C$, stem-bromelain은 pH 7.0과 $60^{\circ}C$ 에서 각각 최대 활성을 나타내었다. 동 식물 유래의 천연단백질을 중심으로 기질 특이성을 검토한 결과 동물성 단백질보다는 밀단백 또는 ISP와 같은 식물성 단백질에 높은 기질 특이성을 보였으며, casein에 대한 fruit와 stem-bromelain의 또 $K_m$값은 0.18 mM와 0.34 mM이었다. pCMB는 경쟁적 저해제로서 fruit와 stem-bromelain의 $K_i$값은 0.18 mM과 0.10 mM이었다. pCMB에 의해 저하된 두 효소의 활성은 cysteine에 의해 재활성화 되었으며 또한 과량의 cysteine 첨가에 의해 활성화됨으로써 이 효소들은 전형적인 thiol계 단백분해효소임을 알 수 있었다.

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Overcoming Encouragement of Dragon Fruit Plant (Hylocereus undatus) against Stem Brown Spot Disease Caused by Neoscytalidium dimidiatum Using Bacillus subtilis Combined with Sodium Bicarbonate

  • Ratanaprom, Sanan;Nakkanong, Korakot;Nualsri, Charassri;Jiwanit, Palakrit;Rongsawat, Thanyakorn;Woraathakorn, Natthakorn
    • The Plant Pathology Journal
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    • 제37권3호
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    • pp.205-214
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    • 2021
  • The use of the supernatant from a Bacillus subtilis culture mixed with sodium bicarbonate was explored as a means of controlling stem brown spot disease in dragon fruit plants. In in vitro experiments, the B. subtilis supernatant used with sodium bicarbonate showed a strong inhibition effect on the growth of the fungus, Neoscytalidium dimidiatum, the agent causing stem brown spot disease and was notably effective in preventing fungal invasion of dragon fruit plant. This combination not only directly suppressed the growth of N. dimidiatum, but also indirectly affected the development of the disease by eliciting the dragon-fruit plant's defense response. Substantial levels of the pathogenesis-related proteins, chitinase and glucanase, and the phenylpropanoid biosynthetic pathway enzymes, peroxidase and phenyl alanine ammonia-lyase, were triggered. Significant lignin deposition was also detected in treated cladodes of injured dragon fruit plants in in vivo experiments. In summary, B. subtilis supernatant combined with sodium bicarbonate protected dragon fruit plant loss through stem brown spot disease during plant development in the field through pathogenic fungal inhibition and the induction of defense response mechanisms.

Enzyme Inhibitory and Antioxidant Activities of Fruit and Stem of Opuntia ficus india var. saboten

  • Han, Yong-Nam;Jun, Jae-Suk;Choo, Yeun-Su
    • 한국응용약물학회:학술대회논문집
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    • 한국응용약물학회 1998년도 Proceedings of UNESCO-internetwork Cooperative Regional Seminar and Workshop on Bioassay Guided Isolation of Bioactive Substances from Natural Products and Microbial Products
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    • pp.187-187
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    • 1998
  • The objective was to verify biological activities of fruit and stem of prickly pear(Opuntia ficus indica L. var, saboten Makino). We have determined inhibitory activities on enzymes, such as dopamine ${\beta}$-hydroxylase(DBH), monoamme oxidase A and B(MAO-A, B), and antioxidant activity, in vitro. We purchased dried stem powder and lyophilized fruit powder of prickly pear from CheJu Island, and prepared the extracts with 80% of methanol. The fruit extract showed stronger inhibitory effects on MAO-A and -B and antioxidant activity compared. to the stem extract, on fractionation with hexane, ethyl acetate, butanol and water. Both the stem and the fruit extracts with ethyl acetate showed stronger enzyme inhibitory and antioxidant activities than other extracts. Now we are isolating active principles from both ethyl acetate extracts.

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천년초선인장의 부위별 영양성분 분석과 정상 및 암세포에 미치는 효과 (Nutrients Contents in Different Parts of Pickly Pear(Opuntia humifusa) and Possible Anti-Breast Cancer Effect)

  • 윤진아;함상욱;손용석
    • 한국식품영양학회지
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    • 제22권4호
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    • pp.485-491
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    • 2009
  • In order to examine the nutritional value of prickly pear(Opuntia humifusa), contents of ash, protein, fat, minerals and vitamins were determined on freeze-dried stem, fruit, seed and root from plants harvested in autumn. The average moisture contents for stem, fruit, seed and root were 67~87%. Crude ash content determined on dry weight basis was 2~3%. Crude protein existed mostly in seed(2.95%) and root(2.37%). Crude fat was detected mainly in seed(4.49%). Contents of major minerals(mg/100 mg dry weight) was generally higher in stem. Ca in stem(4,142.30) and fruit(2,790.86) were much higher than in seed(43.37). P in stem, seed and fruit were 448.19, 263.20 and 161.59, respectively. Stem also displayed more abundant Mg(1,110.86), Zn(35.62) and Mn(37.07). However, fruit contained higher amounts of Fe(13.38) and Se(0.15). Vitamin A was negligible in all plant parts. Vitamin E contents in fruit and stem were 1.78 mg and 1.22 mg/mg dry weight, respectively. Vitamin C was detected mostly in fruit(445.40) and stem(260.94). Use of a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide-based microtiter assay of cell viability demonstrated an anti-proliferative effect of O. humifusa extract on the MCF-7 estrogen-dependent human breast cancer cell line.

복분자 열매, 잎, 줄기 추출물의 콜레스테롤과 혈압 개선 효과 (Improvement of Cholesterol and Blood Pressure in Fruit, Leaf and Stem Extracts from Black Raspberry in vitro)

  • 이민정;이수정;최혜란;이정현;권지웅;채규서;정종태;이태범
    • 한국약용작물학회지
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    • 제22권3호
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    • pp.177-187
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    • 2014
  • We compared effects of fruit, leaf, and stem extracts from black raspberry on improvement of cholesterol and blood pressure in HepG2 and HUVEC cells, respectively. Cholesterol secretion was inhibited by water extracts of unripe fruit and stem, but not leaf of black raspberry in HepG2 cells. Also, water extracts of unripe fruit, leaf, and stem reduced HMG-CoA reductase activity. Furthermore, nitric oxide production and expression of angiotensin-converting enzyme (ACE) protein were regulated by extracts of fruit, leaf, and stem of black raspberry in HUVEC cells. Overall, the rank order according to the improving level of cholesterol and hypertension is as follows: stem > fruit > leaf. In addition, various polyphenol compounds displayed inhibitory effects of HMG-CoA reductase activity and ACE expression. Thus, these data suggested that leaf and stem as wells as fruit of black raspberry can be used as useful food resources for reduction of cholesterol and blood pressure.

손바닥 선인장의 성분 특성 (Compositions of Opuntia ficus-indica)

  • 이영철;황금희;한동휴;김성대
    • 한국식품과학회지
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    • 제29권5호
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    • pp.847-853
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    • 1997
  • 손바닥 선인장(Opuntia ficus-indica var. saboten)의 성분특성을 조사하여 식품으로의 이용 가능성을 검토하고자 하였다. 선인장과 알로에의 주성분은 가용성 무질소물이었다. 총무기질 함량은 선인장 줄기, 열매와 씨가 각각 9400.8, 6151.2와 1096.8 mg%이며, 주요 무기질은 Ca, P, Mg이었다. 주요 유리 아미노산은 선인장 열매인 경우 tyrosine, proline과 arginine이, 줄기는 glycine과 arginine이 씨의 경우 glutamic acid이었다. 총 아미노산중 주요 아미노산은 열매의 경우는 glutamic acid가, 줄기는 glycine과 arginine이었으며, 씨는 glutmic acid와 arginine이었다. 비타민 C는 열매와 줄기가 각각 163.8과 71.2 mg% 존재하나 씨에는 존재하지 않았으며, 비타민 A는 모든시료에서 검출되지 않았다. 총 폴리페놀 화합물과 플라보노이드 함량은 추출방법에 따라 다르며, 총 폴리페놀 화합물의 경우 선인장 열매가 줄기와 씨보다 함량이 높았으며, 총 플라보노이드 함량은 줄기와 열매에서 비슷하였다.

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Measurement of Mechanical and Physical Properties of Pepper for Particle Behavior Analysis

  • Nam, Ju-Seok;Byun, Jun-Hee;Kim, Tae-Hyeong;Kim, Myoung-Ho;Kim, Dae-Cheol
    • Journal of Biosystems Engineering
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    • 제43권3호
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    • pp.173-184
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    • 2018
  • Purpose: This study was conducted to investigate the mechanical and physical properties of a Korean red pepper variety for particle behavior analysis. Methods: Poisson's ratio, modulus of elasticity, shear modulus, density, coefficient of restitution, and coefficient of friction were derived for "AR Legend," which is a domestic pepper variety. The modulus of elasticity and Poisson's ratio were measured through a compression test using a texture analyzer. The shear modulus was calculated from the modulus of elasticity and Poisson's ratio. The density was measured using a water pycnometer method. The coefficient of restitution was measured using a collision test, and the static and dynamic friction coefficients were measured using a inclined plane test. Each test was repeated 3-5 times except for density measurement, and the results were analyzed using mean values. Results: Poisson's ratios for the pepper fruit and pepper stem were 0.295 and 0.291, respectively. Elastic moduli of the pepper fruit and pepper stem were $1.152{\times}10^7Pa$ and $3.295{\times}10^7Pa$, respectively, and the shear moduli of the pepper fruit and pepper stem were $4.624{\times}10^6Pa$ and $1.276{\times}10^7Pa$, respectively. The density of the pepper fruit and the pepper stem were $601.8kg/m^3$ and $980.4kg/m^3$, respectively. The restitution coefficients between pepper fruits, pepper stems, a pepper fruit and a pepper stem, a pepper fruit and plastic, and a pepper stem and plastic were 0.383, 0.218, 0.277, 0.399, and 0.148, respectively. The coefficients of static friction between pepper fruits, pepper stems, a pepper fruit and a pepper stem, a pepper fruit and plastic, and a pepper stem and plastic were 0.455, 0.332, 0.306, 0.364, and 0.404, respectively. The coefficients of dynamic friction between a pepper fruit and plastic and a pepper stem and plastic were 0.043 and 0.034, respectively.

Potential antifilarial activity of the fruit, leaf and stem extract of Melia azedarach Linn. on cattle filarial parasite Setaria cervi in vitro

  • Ahmed, Qamar U.;Zaidi, S.M.K.R.;Kaleem, M.;Khan, N.U.;Singhal, K.C.
    • Advances in Traditional Medicine
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    • 제6권2호
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    • pp.144-156
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    • 2006
  • The effect of aqueous and alcoholic extracts of the fruit, leaf and stem of Melia azedarach Linn. (Meliaceae) on the spontaneous movements of both the whole worm and the nerve-muscle (n.m.) preparation of Setaria (S.) cervi and on the survival of microfilariae in vitro was studied. Alcoholic extracts of fruit, leaf and stem caused inhibition of the spontaneous movements of the whole worm and the n.m. preparation of S. cervi, while only aqueous extract of fruit caused inhibition of the spontaneous movements of the whole worm and the n.m. preparation of S. cervi. The initial stimulatory effect was not observed by the aqueous and alcoholic extracts of fruit on n.m. preparation. The concentrations required to inhibit the movements of the whole worm and n.m. preparation for alcoholic extracts of fruit, leaf and stem were 250, $40\;{\mu}g/ml$; 280, $40\;{\mu}g/ml$ and 270, $25\;{\mu}g/ml$ respectively, whereas an aqueous extract of fruit caused inhibition of whole worm and n.m. preparation at $200\;{\mu}g/ml$ and $40\;{\mu}g/ml$ respectively. Alcoholic extracts of the fruit, leaf and stem and aqueous extract of the fruit of M. azedarach caused concentration related inhibition on the survival of microfilariae (m.f.) of S. cervi. The $LC_{50}$ and $LC_{90}$ as observed after 6 h were found to be 5, 15, 10, 20 ng/ml and 10, 25, 20 and 35 ng/ml, respectively. This work was conducted in view of the exploration of potential antifilarial herbal drug.

가지 F1세대의 잡종강세 정도 (Degree of Heterosis and Heterobeltiosis in F1 Hybrids of Eggplant)

  • 오주성;황필성;정원복
    • 생명과학회지
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    • 제15권1호
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    • pp.132-135
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    • 2005
  • 가지 육종을 위한 기초 자료를 얻고자 9품종을 교잡하여 $F_l$ 세대의 11개 조합을 재료로 각 형질들의 측정치로서 우성의 정도와 잡종강세를 평가하였다. $F_l$세대의 잡종강세는 초장, 경경, 과중, 엽장, 엽폭, 주당과수 등이 초우성으로 유전되었다. 그리고 과장과 과경은 불완전 우성으로 유전되었다. Heterosis의 정도는 초장, 경경, 과중, 과장, 과경, 엽장, 엽폭, 주당과수 등 8개 형질 모두에서 정(+)의 강세를 보였다. Heterobeltiosis의 정도는 초장만이 정(+)의 강세를 보였고, 과경, 엽폭, 엽장, 과중, 주당과수, 과장, 경경은 부(-)의 잡종강세를 보였다

헛개나무의 부위별 이화학적 및 관능적 품질 특성 (Physicochemical and Sensory Characteristics of Extract from Leaf, Fruit Stalk and Stem of Hovenia dulcis Thunb)

  • 박금순;김향희
    • 동아시아식생활학회지
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    • 제15권1호
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    • pp.65-70
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    • 2005
  • This study was conducted in order to promote the utilization of Hovenia dulcis Thunb as food. The physicochemical properties and sensory characteristics of the extracts from the leaf, fruit, stalk and stem were examined. The extract of stem was the brightest in L value (p<0.05), but the lowest in b value (p<0.01). By the sensory evaluation, the external appearance was the best in the extract of stem. The extract of leaf was the darkest in color and that of stem the lightest. The bitter and astringent taste was stronger from the leaf compared with other parts. The extract of fruits stalk of Korean Hovenia dulcis was preferred in most of attributes. The total sugar content was the highest from the fruits stalk. Among free amino acids, aspartic acid, glutamic acid and alanine were rich in order. Potassium contents were the highest among minerals from the fruits stalk, leaf and stem. Ca and Mg followed the next. The results were the same both in Korean and in Chinese tree. Among organic acids, malic acid was the most in the fruits stalk, leaf and stem. It was followed by citric acid. In the test of free sugar, glucose was the most and followed by sucrose and fructose in the fruits stalk. Fructose was contained the most in leaf and stem.

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