• Journal of Plant Biotechnology
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• v.42 no.1
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• pp.25-33
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• 2015

A PyrcpPR-10 gene with differentially expressed was isolated by using the suppression subtractive hybridization assay between '93-3-98' (highly resistant against scab caused by Venturia nashicola) and 'Sweat Skin'(highly susceptible) and analyzed the expression pattern according to organs and cultivars. The full length of PyrcpPR-10 was cloned as 743bp with 480bp's ORP, and was determined to encode a protein of 159 amino acid residues. On analyzing PyrcpPR-10 gene sequence compared with resistant and susceptible cultivars, 'Hwangsilri' (resistant), 'Gamcheonbae' (moderately resistant), 'Wonhwang' (moderately susceptible), 'Niitaka' (highly susceptible), and 'Sweat Skin' (highly susceptible) had identical gene sequence but 'Bartlett' (highly resistant) showed partly different sequences. The deduced amino acid sequence showed 64 ~ 98% homology and had the GXGGXG motif to known amino acid of other plants PR-10 by the BLAST X analysis. Among several organs or tissues, petal was showed highest expression level of PyrcpPR-10 gene followed by leaf, floral axis, bud, and bark. The expression level of PyrcpPR-10 gene was dramatically increased at 24 hr after inoculation in all cultivars and also up-regulated in accordance with resistant degree of cultivars. While resistant cultivars ('Bartlett', '93-3-98', and 'Hwangsilri') induced relatively high expression level of PyrcpPR-10 gene, susceptible cultivars ('Niitaka', and 'Sweat Skin') showed low expression level. PyrcpPR-10 gene is assumed that it is directly connected with defense mechanisms to pear scab.

• Horticultural Science & Technology
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• v.31 no.1
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• pp.32-37
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• 2013

Relationships among climate changes, early frost, and fruit growth were studied during the final month to harvest of late-maturing 'Fuyu' persimmon (Diospyros kaki) to assess the changes in fruit characteristics during this critical period. The heavy frost on Nov. 16 defoliated more than 70% of the leaves, but with little damage on the fruits. However, all the leaves were defoliated by the heavy frost on Nov. 20, and all the fruits were cold-damaged by $-3.3^{\circ}C$ on Nov. 21. Fruit weight increased by 8-25 g per week from Oct. 25 (142 days after full bloom) to Nov. 15, reaching to 250 g, but it decreased by 3-4 g per week after the frost. Hunter a value of fruit skin gradually increased until the last harvest on Nov. 29 with a temporary halt in early Nov. when temperature was high, whereas fruit firmness rapidly decreased after the frost on Nov. 21. Fruit soluble solids were $15.7-16.1^{\circ}Brix$ for the final month. When some branches were covered with non-woven fabrics to avoid direct contact with frost, the fruits on the branches were not visually damaged by the low temperature although 40-60% of their leaves were defoliated on Nov. 16. However, low temperature on Nov. 20 and 21 defoliated all the leaves, causing cold damage on the fruits. There was a highly significant correlation between the fruit diameter and its weight ($R^2$ = 0.73-0.91). So, the regression equations could be used to estimate weight from diameter of the fruits sampled from the branches with the non-woven fabrics. The calculated fruit weight reached to a maximum of 240 g on Nov. 15. Daily increases in fruit weight were 1.1-2.5 g from Oct. 25 to 31, 1.9-3.5 g from Oct. 31 to Nov. 7, and 1.4-1.6 g from Nov. 7 to 12. However, fruit weight decreased by 0.3-1 g per day after the cold damage on Nov. 21. The results indicate that the most appropriate harvest time could be dependent on relationship of fruit growth to climate.

• Horticultural Science & Technology
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• v.28 no.4
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• pp.585-592
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• 2010

This study was conducted to investigate the effect of 1-MCP, an ethylene action inhibitor, and several postharvest treatments including ethylene scrubbing on fruit quality and respiration for keeping marketability in 'Janhowon Hwangdo' peach ($Prunus$ $persica$ Batsch). 1-MCP at the rate of $1.0{\mu}L{\cdot}L^{-1}$ showed best results in maintenance of fruit firmness and external appearance such as skin color. The ethylene production was strongly reduced by 1-MCP treatment at 0.5 or $1.0{\mu}L{\cdot}L^{-1}$ but respiration rate was only suppressed at $1.0{\mu}L{\cdot}L^{-1}$ during 6 days of shelf life at $20^{\circ}C$. Mature fruits (harvested 1 week before full commercial maturity) were much highly responsive to $1.0{\mu}L{\cdot}L^{-1}$ of 1-MCP compared to those of commercial maturity. At the concentration of $0.5{\mu}L{\cdot}L^{-1}$ of 1-MCP did not affect fruit marketability compared to untreated control. Beneficial effects of carbon ceramic as an ethylene scrubber were also found such as delay of firmness loss and deterioration of external appearance at $10^{\circ}C$, but these positive effects on fruit quality remained for only 5 days. The application of ethylene scrubber on the shipping carton boxes was effective on keeping firmness of immature fruit pretreated with 1-MCP when compared with mature fruit.

• Horticultural Science & Technology
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• v.29 no.6
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• pp.655-658
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• 2011

A new cultivar 'Hongan' was originated from an artificial cross between 'Fuji' and 'Jonathan' carried out at National Institute of Horticultural & Herbal Science in 1993. The cultivar was preliminarily selected among the elite siblings for its high fruit quality in 2002. After regional adaptability test at five districts for subsequent four years as 'Wonkyo Ga-32', it was finally selected in 2006. 'Hongan' is characterized by the following. Optimum harvest time is late September. Shape of the mature fruit at 309 g on mean weight is globosity with a light red skin on a greenish yellow ground and yellowish white flesh. The fruit contains favorable total soluble solids at $13.7^{\circ}Brix$ and an acidity at 0.28%. Safe storage period for the fruit is 3 weeks at room temperature. It is resistant to Bitter rot. 'Hongan' also reveals a physiological cross compatibility with leading cultivars such as 'Gamhong' and 'Tsugaru' in addition to its maternal parent 'Fuji'. Tree topology is semi-spreading with vigorous growth habit.

• Korean Journal of Plant Resources
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• v.32 no.4
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• pp.303-311
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• 2019

Various colors of fruit skin and flesh are the most popular commercial criteria for peach classification. In order to breed new red-fleshed peach cultivar, many cross seedlings and generations should be maintained. Therefore it is necessary to develop early selection markers to screen seedlings with target traits to increase breeding efficiency. For the comparison of transcription profiles in peach cultivars differing in flesh color expression, two cDNA libraries were constructed. Differences in gene expression between red-fleshed peach cultivar, 'Josanghyeoldo' and white-fleshed peach cultivar, 'Mibaekdo' were analyzed by next-generation sequencing (NGS). Expressed sequence tag (EST) of clones from the two cultivars were selected for nucleotide sequence determination and homology searches. Putative single nucleotide polymorphisms (SNP) were screened from peach EST contigs by high resolution melting (HRM) analysis displayed specific difference between 8 red-fleshed peach cultivars and 24 white-fleshed peach cultivars. All 72 pairs of SNPs were discriminated and the HRM profiles of amplicons were established. In the study reported here, the development of SNP markers for distinguishing between red and white fleshed peach cultivars by HRM analysis offers the opportunity to use DNA markers. This SNP marker could be useful for peach marker assisted breeding and provide a good reference for relevant research on molecular mechanisms of color variation in peach cultivars.

• Korean Journal of Breeding Science
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• v.50 no.4
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• pp.497-503
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• 2018

A strawberry variety 'Arihyang' was derived as an artificial cross between 'Tochiotome' and 'Seolhyang' in 2014. The seedling and line selections were conducted from 2014 to 2015. Preliminary and advanced yield trials of '14-5-5,' which was the final selected line, were conducted from 2015 to 2017. 'Arihyang' is suitable for forced cultivation and has strong plant vigor, uniformly large-sized fruit, and a high yield compared to those of the check variety, 'Seolhyang' and 'Maehyang.' Especially, vitamin C was at a significant level, which was approximately 15% higher than that of 'Seolhyang.' The average number of flowers per first flower cluster was 10.5, which could reduce the labor of thinning fruit. Its fruit has a conical shape, dark red color, and glossy skin. The fruit was of good quality but has recommendations for harvest at the fully ripened stage. 'Arihyang' has intermediate resistant to phytophthora crown rot, but is susceptible to powdery mildew, gray mold, anthracnose, and fusarium wilt. It is reguired to manage major diseases and pests using optimum cultivation techniques and chemical control.

• Korean Journal of Agricultural and Forest Meteorology
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• v.23 no.4
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• pp.349-355
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• 2021

The effect of night temperature was investigated on mango (Mangifera indica cv. Irwin) for setting appropriate night temperature and managing mango cultivation in a greenhouse. Different night temperatures (10, 15, and 20℃) were treated from budding to fruit development in mango trees. As night temperature increased, the initiation of flowering, full blooming, fruiting, and fruit development tended to be accelerated. There were no significant differences in the growth of shoots flushed after the fruit harvest among trees treated with different night temperatures. The lengths of the flower stalk were the shortest at 10℃ of night temperature; however, there were no significant differences between 15℃ and 20℃ of the night temperature. The length, diameter, and weight of mango fruits were higher at 15℃ and 20℃ than 10℃. Soluble solid contents and acidities showed no difference; the firmness and skin color were better as the night temperature increased. Accordingly, setting the minimum night temperatures to 15℃ or 20℃ can increase the number of fruits and produce high-quality fruits when cultivating mango in the greenhouse. Even the minimum night temperature to 15℃ can reduce heating costs in the greenhouse.

• Food Science and Preservation
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• v.20 no.6
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• pp.846-853
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• 2013

We investigated the effect of the cell-wall-degrading enzyme and its skin contact time on the brewing characteristics of Cheongsoo grape. The easy of juice extraction was excellent at the cell-wall-degrading enzyme and skin contact treatments, and the aroma was best after five days of skin contact treatment. Furthermore, the juice yields of the Chengsoo grape were more dramatically increased by the cell-wall-degrading enzyme and skin contact treatments than by the control. The data on the pH, total acidity, and soluble solids did not significantly differ among the treatments, and the pH range from 3.1 to 3.4; the total acidity from 0.5% to 0.6% (as tartaric acid); and the soluble solids, from 6.7 to 7.1 $^{\circ}Brix$. The alcohol content of cell-wall-degrading enzyme treatment was highest with 13.3%. The total polyphenol was gradually increased with the longer skin contact time, and was highest after 10 days of skin contact treatment, at 306.4 mg/L. The main organic acids detected in the Cheongsoo wine were malic and tartaric acid, and citric, succinic and lactic acid were also detected. Our results show that the cell-wall-degrading enzyme and skin contact treatments were better in terms of the easy of juice extraction and significantly increased the juice yield and the volatile compound of the Cheongsoo wine.

• Proceedings of the SCSK Conference
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• 2003.09a
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• pp.672-685
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• 2003

Yew (Taxus cuspidata Sieb.) chose that grow as medicine, food, decorative plant in Korea's Kyong-Gi province surroundings. Extracts of yew extracted leaf of 250 g and stems of 300 g with 1,3-butylene glycol (1,3-BG), propylene glycol (PG) and water. As results, external appearance of leaf extract of yew was slightly brown clear extract. The pH was 5.3$\pm$0.5, and specific gravity was 1.012$\pm$0.05, and refractive index was 1.375$\pm$0.05. Also, appearance of stem's extract was slightly brown clear extract, and the pH was 5.4$\pm$0.5, and specific gravity was 1.016$\pm$0.05, and refractive index was 1.358$\pm$0.05. Oil of yew separated from seeds, and extracted polysaccharide high purity from fruits. As a result, specific gravity of oil was 0.987, and obtained 40％ of yield. Total polyphenols amount of yew extract is detected 0.563％ in leaves, 0.325％ in stems, whereas total tannins amount contained 0.054％ and 0.037％ each in leaves and stems. As effect in cosmetics, the anti-oxidative effect by DPPH method is 75.0％ in leaves, and stems was 64.0％. Collagen synthesis rate was shown high activity by 54.16％ in stem's extract, 33.18％ in leaves' extract. Also, PPE-inhibitory activities were 13.7％ and 23.5％ each in leaves and stems. Anti-inflammatory effect of yew seed oil displayed superior effect of 41 ％ than control. Polysaccharide's molecular weight that is gotten from fruits was 5$\times$10$^4$~ 3$\times$10$^{5}$ dalton, and got 20.0$\pm$5％ of yield.

• KSBB Journal
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• v.31 no.4
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• pp.256-262
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• 2016

The need for natural cosmetic ingredients has been increasing over the world nowadays. Agarose, a natural polymer from red seaweeds, has high hydrophilic character and a function of scaffolder. As skin moisturizer, agarose is adequate for percutaneous absorption. While, blueberry fruits extract possesses rich procyanidins and anthocyanins which show health benefits, anti-oxidant effect, anti-aging and anti-melanogenesis. Stability, sensory preference, skin trouble of the emulsion formula are important for cosmetic product development. In this study, we manufactured an emulsion formula for skin moisturizers using the two ingredients and tested emulsion stability and skin trouble. Total phenolic contents of the blueberry fruits extract were evaluated as well as tyrosinase inhibitory and collagenase inhibitory activities. $IC_{50}$ values of blueberry fruits extract for anti-tyrosinase and anti-collagenase activities were 168 and $112{\mu}g/mL$, respectively using gallic acid as a control ($64.8{\mu}g/mL$). The stability (pH and viscosity) of the formula containing 2% blueberry fruits extracts and 0.1% agarose was measured at five different temperatures (room temp., $25^{\circ}C$, $55^{\circ}C$, $45^{\circ}C$, $55^{\circ}C$) under the sun light at 2 day intervals for 12 days. There has been little pH change at the different temperatures. According to the sensory evaluation, there was no significant flavor, discoloration and physical changes of the formula at $25-65^{\circ}C$. These results suggest that emulsion formula containing blueberry extract and agarose could be used as a candidate for lotion and essence products.