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Effect of Deep Sea Water on Seed Germination, Photoperiod and Temperature on the Growth and Flowering of Buckwheat Species

  • Briatia, Xoxiong;Hong, Soon-Kwan;Sung, In-Je;Chang, Kwang-Jin;Park, Byoung-Jae;Park, Cheol-Ho
    • Korean Journal of Plant Resources
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    • v.25 no.3
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    • pp.323-328
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    • 2012
  • This paper describes the potential use of deep sea water to stimulate seed germination in both common and Tartary buckwheat. Treatment of 10% deep sea water at $25^{\circ}C$ would slightly enhance germination of buckwheat seeds compared to non-DSW treatment and other temperature. In this study, the significant effects of photoperiod and temperature on seedling growth were also found in the HL treatment for the number of leaf, plant height, and plant fresh weight and LL treatment for root length and leaf size. Common buckwheat (Suwon No.1) showed higher rate (93%) of flowering plants in the HS and LL (93% of flowering rates) than those revealed in the HS and LS treatment, while the low percentage(67%) of plant flowering plants was shown in the LS treatment. All plants (100%) of a Korean landrace, Ahndong-jaerae showed flowers in the HS and LS treatment. HL and LL treatment status did not occur in the plant's flowering. Any Tartary buckwheat (KW45) plant did not yet flowered when it was 21 days-old.

Light and Electron Microscopy Studies Elucidating Mechanisms of Tomato Leaf Infection by Pseudocercospora fuligena

  • Zelalem Mersha;Girma Birru;Bernhard Hau
    • The Plant Pathology Journal
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    • v.39 no.2
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    • pp.181-190
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    • 2023
  • The fungal pathogen Pseudocercospora fuligena, known to affect tomatoes in the tropics and subtropics, has been reported from temperate climates including the United States and Turkey in recent years. In this study, an isolate from fresh tomatoes and the disease it causes were characterized and infection mechanisms investigated. Macroscopically, both sides of tomato leaves show indistinct effuse patches but prolific production of fuliginous lesions is conspicuous on the abaxial side first but also on the adaxial side later on as infection progressed. Microscopically, fascicles of conidiophores (11-128 ㎛ × 3.5-9 ㎛) arising from stromata and conidia with up to 12 septations were observed. Molecular characterization of the isolate revealed high homology (99.8%) to other P. fuligena isolated from tomatoes in Turkey. Out of the 10 media tested, P. fuligena grew significantly well and sporulated better on unsealed tomato oatmeal agar and carrot leaf decoction agar, both supplemented with CaCO3. Direct transfer of conidia from profusely sporulating lesions was the easiest and quickest method of isolation for in-vitro studies. Light and scanning electron microscopy on cleared and intact tomato leaves further confirmed stomatal penetration and egress as well as prevalence of primary and secondary infection hyphae. In situ, blocked stomatal aperture areas of 154, 401, and 2,043 ㎛2 were recorded at 7, 12, and 17 days after inoculation, respectively. With the recent expanded horizon of the pathosystem and its consequential impact, such studies will be useful for a proper diagnosis, identification and management of the disease on tomato worldwide.

Seedling Qualities of Hot Pepper according to Seedling Growth Periods and Growth and Yield after Planting (육묘 기간에 따른 고추 묘의 소질과 정식 후 생육 및 수량)

  • Kim, Ho Cheol;Cho, Yun Hee;Ku, Yang Gyu;Bae, Jong Hyang
    • Horticultural Science & Technology
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    • v.33 no.6
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    • pp.839-844
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    • 2015
  • This study was carried out to investigate seedling quality, growth characteristics and yield of hot pepper (Capsicum annum) grown in the open field according to seedling growth periods (SGPs) of 45, 55, 65, 75, and 85 days. Before planting, plant height, node number, leaf area, fresh and dry weight of seedlings were high in longer-SGP treatments, the T/R ratio was high in SGP 45 and SGP 85 treatments compared with other treatments. At 10 weeks after planting, plant height, stem diameter and leaf area of plants treated with SGP 45 and SGP 55 were significantly higher compared to other treatments. Fresh and dry weight of the plant with SGP 45 treatment was greatest, however, the dry matter percentage with SGP 45 was low compared to other treatments. Fruiting number and weight per plant were highest in SGP 45 treatment. Shorter SGP treatments such as 45 and 55 days gave greatly increased total weight of ripened fruit at 18 weeks after planting. Our results showed that SGP for hot pepper grown in the open field influences plant growth parameters and marketable yield, so that SGP 45 to SGP 55 is optimum to cultivate hot pepper plant.

The Nutritive Value of Mulberry Leaves (Morus alba) and Partial Replacement of Cotton Seed in Rations on the Performance of Growing Vietnamese Cattle

  • Vu, Chi Cuong;Verstegen, M.W.A.;Hendriks, W.H.;Pham, K.C.
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.9
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    • pp.1233-1242
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    • 2011
  • The in vivo digestibility of mulberry leaves (Morus alba) and the effects of the partial replacement of cotton seed with fresh mulberry leaf in rations on the performance of growing Vietnamese cattle was investigated. For the in vivo digestibility trial, twenty castrated rams of Phanrang breed (a local prolific breed) with an initial weight of 23-25 kg, were first assigned to four groups according to weight and then randomly assigned to one of four dietary treatments to determine digestibility of nutrients in mulberry leaves (M. alba), natural Bermuda grass (Cynodon dactylon), elephant grass (Pennisetum purpureum) and buffalo grass (Panicum maximum cv. TD 58). All forages were cut and chopped daily before being offered (at 120% maintenance) to the sheep. In the feeding trial, 20 Laisind (Vietnam yellow cows${\times}$Red Sindhy bulls) crossbred bulls averaged 18 month old and 184 kg were used to investigate the effect of partial replacement of cottonseed in the diet by mulberry leaves on live weight gain and feed conversion rate. The experiment was a randomized complete block design with four levels of fresh mulberry leaves which varied from 0 to 15% of total dietary dry mater and five animals per treatment over an 84 day period. The in vivo digestion trial showed the superior quality of mulberry leaves compared with the grasses. Chemical analysis indicated that mulberry leaves had the highest CP and the lowest NDF contents (22.3 and 31.1% DM, respectively) among the four forages tested. Digestibility of DM and OM of the mulberry leaf (66.4 and 71.8%, respectively) was also the highest but that of CP (58.2%) and NDF (58.4%) was the lowest of the four forages evaluated (p<0.05). Consequently, the ME value and therefore net energy (NE) and unit feed for lactation (UFL) values of the mulberry leaves, which was estimated from chemical composition and digestibility values, were the highest among the forages investigated in the present study. Results of the feeding trial showed no treatment effect on average daily gain (ADG) of the cattle. The values were 554, 583, 565 and 568 g/d for animals in the diets of 0, 5, 10, and 15% mulberry leaves inclusion, respectively. Total DM intake of the animal was not affected by the treatment when expressed as kg/animal/d. However, when adjusted for metabolic weight of the animal the DM intake was reduced (p<0.05) as whole cottonseed was replaced by mulberry leaves in the ration. When the level of mulberry leaves in the ration increased from 5 to 15% of dietary DM at the expense of whole cottonseed, CP and ME intakes of the cattle were significantly decreased (p<0.05) and the feed to gain ratio reduced by 8 to 14% as compared with the control diet (p<0.05). Mulberry leaf is a good feed ingredient for ruminants because of its high level of crude protein and high digestibility of nutrients and energy. Mulberry leaves can be efficiently used as a source of protein supplement to replace cottonseed, a more expensive animal feeds ingredient, in the diet for Vietnamese cattle.

Comparison of Growth Characteristics and Yield of Soybean Varieties for Soiling Crop Improvement (풋베기용 대두 개량을 위한 품종의 생육특성 및 수량 비교)

  • Lee, Sung-Kyu;Choi, Il;You, Jae-Yeul
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.19 no.4
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    • pp.309-316
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    • 1999
  • This Study was carried out to compare growth characteristics and yield of 10 cultivated soybean varieties, Jinpum I, Jinpum II, Hwanggum, Gumjung I, Gumjung II, Seukryangboot, Jangyup, Gumjungol, Bokwang, Manri for improvement of soiling crop. Plant height of Gumjung I(118cm), Gumjung II(114cm) and Jinpum I(114cm) were taller than other varieties based on dough stage. In total fresh weight and DM yield of Jinpum I, Jinpum II, Gumjung II were the highest of all varieties as 23,841kg, 23,499kg and 22,815kg per hectare, and 5,531kg, 5,173kg and 5,236kg per hectare, respectively. The highest leaf/stem ratio of ten soybean varieties were Manri(2.1), Jinpum I(1.9) and Hwanggum(1.9). Except early matured varieties, Seukryangboot(25.9%) and Gumjungol(25.4%), dry matter percent at dough stage were in the range of 22.0~23.4%. According to plant height, fresh weight, dry matter yield, and leaf/stem ratio, Jinpum I and Gumjung II were proper varieties to improve soiling crop.

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Comparison of Volatile Compounds in Plant Parts of Angelica gigas Nakai and A. acutiloba Kitagawa (참당귀와 일당귀의 부위별 휘발성 정유성분 비교)

  • Cho, Min-Gu;Bang, Jin-Ki;Chae, Young-Am
    • Korean Journal of Medicinal Crop Science
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    • v.11 no.5
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    • pp.352-357
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    • 2003
  • Volatile flavor compounds Angelica gigas Nakai and Angelica acutiloba Kitagawa were extracted by SDE (simultaneous steam distillation & extraction) using the mixture of n-pentane and diethylether (1:1, v/v) as an extract solvent and analyzed by GC-FID and GC-MS. The amount of essential oils of top part and root in Angelica gigas were obtained in 0.063% (v/w) and 0.389% (v/w) yields as a fresh weight base, respectively. The main compounds in top parts and the root were identified as nonane (7.51% and 24.49%, respectively), ${\alpha}-pinene$ (14.64% and 31.75%), limonene+${\beta}-phellandrene$ (14.01% and 9.66%), ${\gamma}-terpinene$ (7.85% and 1.20%), germacrene-d (5.85% and 0.22%), (E,E)-${\alpha}-farnesene$ (6.05% and 1.40%), ${\beta}-eudesmol$ (5.26% and 1.84%). Although these compounds were present in both parts. The results showed large differences in. the concentrations of them much varied. The amount of essential oils stem and leaf obtained (0.068% and 0.127% in A. gigas) and (0.153% and 0.243% in A. acutiloba) yields as a fresh weight base, respectively. More than 18 and 32 components in stem and leaf have been identified, which of main components in A. gigas were ${\alpha}-pinene$, myrcene, limonene, germacrene-d, eudesmol and butylphthalide, but germacrene-d and butylphthalide contents were also different in stem and leaf. And more than 21 and 32 components in A. acutiloba were ${\gamma}-terpinene$ and butylphthalide. Volatile compounds were very different in both species.

Physicochemical Composition of Head-Type Kimchi Cabbage Leaves (결구배추 잎의 이화학적 성분 조성)

  • Seong, Gi-Un;Hwang, In-Wook;Chung, Shin-Kyo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.6
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    • pp.923-928
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    • 2016
  • To increase the consumption of head-type kimchi cabbage leaves as fresh food ingredients, the physicochemical composition was investigated depending on leaf parts. Kimchi cabbage leaves were divided into three portions, such as outer (L1), mid (L2), and inner (L3), and their proximate compositions were determined along with dietary fiber, free sugar, organic acid, free amino acid, and mineral contents. Contents of moisture, crude protein, and crude fat were highest in L1, whereas contents of crude ash and carbohydrate were highest in L3 (P<0.05). Calories ranged 24.5~26.5 kcal/100 g with no significant difference among leaf parts, and dietary fiber content of L1 was highest (P<0.05). Fructose, glucose, and sucrose contents were highest in L3 (P<0.05). Citric acid, malic acid, and succinic acid were detected, and contents of citric acid and malic acid were highest in L3, in contrast, succinic acid was highest in L1 (P<0.05). Glutamic acid, constituting over 30% of the total free amino acid contents, was highest in L3, whereas ${\gamma}-aminobutyric$ acid contents were highest in L1 (P<0.05). K, Na, Ca, and Mg were the major minerals in head-type kimchi cabbage, and their contents showed no significant differences (P<0.05). It can be proposed that marketing of head-type kimchi cabbage as a fresh-cut vegetable could be enhanced due to the different quality characteristics of its leaf parts.

Lateral Root Formation and Growth of Soybean Sporouts Treated with Various Solutions (다양한 물질처리에 의한 콩나물의 세근형성 및 생장)

  • Kang, Jin-Ho;Park, Cheol-Jong;Yoon, Soo-Young;Jeon, Seung-Ho;Hong, Dong-Oh
    • Korean Journal of Medicinal Crop Science
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    • v.13 no.1
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    • pp.6-10
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    • 2005
  • Lateral roots of soybean sprouts should deeply affect their quality and marketability. The study was done to compare the effects of ocher, chitosan, corn kennel, tea (Thea sinensis L.) and hard rubber tree (Eucommia ulmoides Oliver) leaf extracts on lateral root formation, growth and morphological characters of the sprouts. Seeds of three cultivars, Pungsannamulkong, Sowonkong and Junjery, were imbibed for 5 hours into their 10% solutions and cultured for 6 days. The cultured sprouts were classified into 4 categories to calculate their composition rates on the base of hypocotyl lengths;>7 cm (A), 4 to 7 cm (B), < 4 cm (C) and not germinated (D), and their morphological characters, fresh and dry weights were measured. Composition rate of A was the lowest in Junjery of the three cultivars, while that of C showed reverse result compared to A. This results was the most distinct in hard rubber tree leaf extracts (HRTLE) of the five treatments. In HRTLE treatment, lateral root formation rate were formed in almost of Sowonkong although reduced in order of Pungsannamulkong and Junjery. However, there was no significant difference between the other treatments. Lateral roots per sprout were the lowest in HRTLE treatment of the 5 treatments. In all treatments except the chitosan treatment, the roots were most formed in Sowonkong but least in Junjery. Sprout length adding hypocotyl and root was the shortest in Junjery compared to the other two cultivars. and was the longest in tea leaf extract treatment but the shortest in HRTLE treatment. The result in total fresh weight of sprouts was similar to that of the sprout length.

Screening for Resistance to Downy Mildew among Major Commercial Cucumber Varieties (주요 오이 품종의 노균병에 대한 저항성 검정)

  • Lee, Jung-Sup;Han, Kyung-Sook;Lee, Seong-Chan;Soh, Jae-Woo
    • Research in Plant Disease
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    • v.19 no.3
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    • pp.188-195
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    • 2013
  • This study was carried out for the downy mildew resistant test between 2010 and 2012. A set of 22 accessions belonging to 2 wild species and 20 varieties of the genus Cucumis, originating mainly from the Asian Vegetable Research and Development Center (AVRDC) Gene Centre, was evaluated for resistance to Pseudoperonospora cubensis, causal agent of cucumber downy mildew. The youngest fully expanded true leaves were found suitable for in vitro screening. Both leaf discs and full leaves could be kept fresh longer when applying 0.2 ${\mu}g/ml$ of gibberellin acid (GA). The incubation temperature of $20^{\circ}C$ was found to be the most suitable temperature for symptom development comparing with 15 and $25^{\circ}C$. Symptom development was faster when contact diseased leaf discs (2 weeks after inoculation) on to fresh leaf samples comparing with using conidia suspension ($10^5$ spores/ml). The numbers of spots in 'C-19' were lower than other varieties. 'C-19' variety was also showed the highest level of downy mildew resistant at $20^{\circ}C$ chamber in 6 days after inoculating with pathogen and displayed 0.90 (under 10%) of the infected rate. However, other varieties displayed susceptible in the pathogen sprayed plots. 'C-19' was the most resistant variety and no lesion was observed. Based on all data, 'C-19' can be a useful variety for the prevention of downy mildew.

Growth and Yield of Sedum sarmentosum as Affected by Planting Density in Cultivation System Using a Rice Nursery Tray (벼 육묘상자를 이용한 돌나물 재배에서 삽식밀도에 따른 생육 및 수량)

  • Kim, Hyo-Jin;Lee, Seung-Yeob
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.53 no.2
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    • pp.196-202
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    • 2008
  • To establish a cultivation system of Sedum sarmentosum using a rice nursery tray ($30{\times}60{\times}3\;cm$) in non-heating plastic film house, cuttings of two local strains (Wanju and Keumsan) were prepared and planted at different planting densities (40, 60 and 80 cuttings per tray) on September 7th, and subsequent growth and yield at pre- and post-winter season were investigated. When pre-wintering growth were measured at 60 days after planting, high planting density (80 cuttings/tray) decreased leaf length and lateral shoot number per plant compared to 40 cuttings/tray. When post-wintering growth were measured on April 20th, no significant growth differences as affected by planting density were observed in Keumsan strain, while decreased stem diameter, node number and leaf number were observed in Wanju strain planted at 80 cuttings/tray compared to 40 cuttings/tray. Fresh and dry weights were not significantly different as affected by planting density, but those were significantly increased in Wanju strain compared to Keumsan strain. When regrowth characteristics followed by the first harvest were measured on June 5th, significantly increased stem number was observed at 80 cuttings/tray compared to 40 cuttings/ tray. Fresh and dry weight were significantly increased in Keumsan strain planted at 80 cuttings/tray compared to 40 cuttings/tray, and dry weight were increased in Wanju strain compared to Keumsan strain. Wanju strain showed higher shoot yield, thicker stem, shorter node and larger leaf, and non-succulent stem under high planting density compared to Keumsan strain. Accordingly, the optimum density was 40 cuttings per tray, and Wanju strain was adequate for the cultivation system using a rice nursery tray.