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http://dx.doi.org/10.5423/RPD.2013.19.3.188

Screening for Resistance to Downy Mildew among Major Commercial Cucumber Varieties  

Lee, Jung-Sup (Horticultural & Herbal Environment Division, National institute of Horticultural and Herbal Science, Rural Development Administration)
Han, Kyung-Sook (Horticultural & Herbal Environment Division, National institute of Horticultural and Herbal Science, Rural Development Administration)
Lee, Seong-Chan (Horticultural & Herbal Environment Division, National institute of Horticultural and Herbal Science, Rural Development Administration)
Soh, Jae-Woo (Horticultural & Herbal Environment Division, National institute of Horticultural and Herbal Science, Rural Development Administration)
Publication Information
Research in Plant Disease / v.19, no.3, 2013 , pp. 188-195 More about this Journal
Abstract
This study was carried out for the downy mildew resistant test between 2010 and 2012. A set of 22 accessions belonging to 2 wild species and 20 varieties of the genus Cucumis, originating mainly from the Asian Vegetable Research and Development Center (AVRDC) Gene Centre, was evaluated for resistance to Pseudoperonospora cubensis, causal agent of cucumber downy mildew. The youngest fully expanded true leaves were found suitable for in vitro screening. Both leaf discs and full leaves could be kept fresh longer when applying 0.2 ${\mu}g/ml$ of gibberellin acid (GA). The incubation temperature of $20^{\circ}C$ was found to be the most suitable temperature for symptom development comparing with 15 and $25^{\circ}C$. Symptom development was faster when contact diseased leaf discs (2 weeks after inoculation) on to fresh leaf samples comparing with using conidia suspension ($10^5$ spores/ml). The numbers of spots in 'C-19' were lower than other varieties. 'C-19' variety was also showed the highest level of downy mildew resistant at $20^{\circ}C$ chamber in 6 days after inoculating with pathogen and displayed 0.90 (under 10%) of the infected rate. However, other varieties displayed susceptible in the pathogen sprayed plots. 'C-19' was the most resistant variety and no lesion was observed. Based on all data, 'C-19' can be a useful variety for the prevention of downy mildew.
Keywords
Cucumis sativus; Fruit number; Germplasm evalution; Vegetable breeding;
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