• Title/Summary/Keyword: frequency effect

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Spontaneous Parthenogenesis of Porcine Oocyte Induced by Prolonged Culture in Various Media (다양한 배지에서 장시간 배양에 의한 돼지 난자의 단위발생 유도)

  • J. M. Koo;Y. J. Ryu;Lee, E. G.;Kim, D. Y.;Kim, S. W.;S. K. Kang;Lee, B. C.;W. S. Hwang
    • Journal of Embryo Transfer
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    • v.18 no.1
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    • pp.69-73
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    • 2003
  • This study was undertaken to investigate the effect of three different porcine IVM media, TCM-199, mSOF and NCSU-23 on early development of porcine spontaneous parthenogenotes. Spontaneous parthenogenotes were induced by a prolonged culture of porcine oocytes in each medium. In Experiment 1, oocytes derived from gilts were matured in three IVM media and maturation of oocytes was evaluated by the status of chromatin configuration. Oocytes matured in mSOF, NCSU-23 and TCM-199 showed no significant difference (P>0.05) in maturation. Maturation rates at 48h after IVM were 83.1$\pm$2% (mSOF), 78.0$\pm$3% (NCSU-23) and 83.5$\pm$2% (mSOF), 78.0$\pm$3% (NCSU-23) and 83.5$\pm$2% (TCM-199). In Experiment 2, pronucleus formation and development to 6~8 cell stage of pig oocytes activated spontaneously. Pronucleus formation, cleavage rate and development to 6~8 cell embryos of porcine spontaneous parthenogenotes were assessed at 55~58 h, 96 h and 168h after IVM, respectively. Pronucleus formation (5.4$\pm$2% and 3.7 $\pm$ 1% vs 1.7 $\pm$ 3%) and development to the 6$\pm$8 cell (3.2$\pm$3% and 4.0$\pm$1% vs 1.4$\pm$3%) was significantly (P<0.05) higher in mSOF or NCSU-23 than TCM-199. In conclusion, the present study showed that oocytes matured in mSOF and NCSU-23 were spontaneously activated with higher frequency.

Effect of a public health center-based nutrition education program for hypertension in women older than 50 years of age (50세 이상 여성을 대상으로 한 보건소 기반 고혈압 영양교육의 효과 평가)

  • Park, Seoyun;Kwon, Jong-Sook;Kim, Hye-Kyeong
    • Journal of Nutrition and Health
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    • v.51 no.3
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    • pp.228-241
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    • 2018
  • Purpose: The health risk of women increases after menopause. This study evaluated the effectiveness of a public health center-based nutrition education program for hypertension in women older than 50 years of age. Methods: The program included 8-week nutrition education and 8-week follow-up with keeping a health diary and nutrition counseling. The program was evaluated three times: before and after the nutrition education, and after the follow-up. The subjects were classified into hypertensives (n = 44) or normotensives (n = 71). Results: The rate of taking antihypertensive drugs in the hypertensive group was 86.4%. The systolic blood pressure decreased in the hypertensive and normotensive groups after nutrition education (p < 0.05). The body weight (p < 0.001), BMI (p < 0.001), waist circumference (p < 0.001), and percent body fat (p < 0.01) were also decreased after nutrition education in both groups. The hypertensive group showed an increase in HDL-cholesterol level (p < 0.001) and decreases in triglycerides (p < 0.01) and LDL-cholesterol (p < 0.05) levels after completion of the program. The normotensive group also displayed significant changes in HDL-cholesterol (p < 0.001) and triglycerides (p < 0.01). The dietary habits and nutrition knowledge on sodium and hypertension were improved in both groups (p < 0.001). The total score of dietary behavior related to the sodium intake was improved in the normotensive group (p < 0.001). The total score of the high sodium dish frequency questionnaire decreased in both groups after nutrition education and completion of the program compared to that before the program. Decreases in the consumption frequencies of noodles, pot stews and stews, Kimchi, and beverages were significant. The total self-efficacy score was increased in both groups by the program (p < 0.001). In particular, the hypertensive group showed improvement in all items. Conclusion: This public health center-based nutrition education program may contribute to the prevention and management of hypertension and chronic diseases in women over 50 years of age.

A Study on the Water Exchange Plan with Disaster Prevention Facilities in Masan Bay (마산만 재해방지시설을 이용한 해수교환 방안에 관한 연구)

  • Kim, Gweon-Su;Ryu, Ha-Sang;Kim, Kang-Min
    • Journal of Navigation and Port Research
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    • v.37 no.6
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    • pp.637-645
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    • 2013
  • Masan bay with a semi-enclosed waters has serious water quality problems due to the low flow and river pollution load from land, and shows the vulnerable locational characteristics to storm surge. We are seeking the way of both operating disaster prevention facilities and water quality improvement measures in the bay. That is, the water was exchanged using the head difference occurred by operating disaster prevention facilities. The location of disaster prevention facilities was assumed to be in the inlet of the bay, in the vicinity of Machang bridge, and in the vicinity of Dot island and the operation time was assumed to be early morning hours(01~05) considering the number of shipping passage and annual tide, and spring tide of the largest head difference. In addition, the experiment case of water exchange including the in-outflow feeder pipe was tested. According to the simulation results, water exchange rate in all experiments has shown a steady increase. Water exchange rate of the whole of Masan bay in the case of present is 38.62%. The water exchange rate of the inside of Masan bay compared with the inlet of bay, appeared to be very low. Thus, we judged that the characteristics of semi-enclosed waters were well reproduced. On the results of the experiment of disaster prevention facilities and in-outflow feeder pipe, the case of the operation of disaster prevention facilities, water exchage rate is high compared with the case of present. And, the higer the operating frequency, the more water exchange is appeared. The cases of water exchange prevention facilities through the in-outflow feeder pipe caused by the head difference, also showed the higest improvement of the water quality. Compared with the south of Machang bridge, the effect of water exchange was better in the inlet of Masan bay and Dot island. On the other hand, the inlet of Masan bay is higer than Dot island as for water exchange of the whole of Masan bay, but opposite, water change rate including Masan inside was higher in the case of Dot island.

Comparison of shaping ability between single length technique and crown-down technique using Mtwo rotary file (Mtwo 전동 파일을 사용한 single length technique과 crown-down technique의 근관성형 효율 비교)

  • Lim, Yoo-Kyoung;Park, Jeong-Kil;Hur, Bock;Kim, Hyeon-Cheol
    • Restorative Dentistry and Endodontics
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    • v.32 no.4
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    • pp.385-396
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    • 2007
  • The aims of this study were to compare the shaping effect and safety between single length technique recommended by manufacturer and crown-down technique using Mtwo rotary file and to present a modified method in use of Mtwo file. Sixty simulated root canal resin blocks were used. The canals were divided into three groups according to instrument and the manner of using methods. Each group had 20 specimens. Group MT was instrumented with single length technique of Mtwo, group MC was instrumented with crown-down technique of Mtwo and group PT was instrumented with crown-down technique of ProTaper. All of the rotary files used in this study were operated by an electric motor. The scanned canal images of before and after preparation were superimposed. These superimposed images were evaluated at apical 1 to 8 mm levels Angle changes were calculated. The preparation time, weight loss, instrument failure and binding, canal aberrations, and centering ratio were measured. Statistical analysis of the three experimental groups was performed with ANOVA and Duncan's multiple range tests for post-hoc comparison and Fisher's exact test was done for the frequency comparison. In total preparation time, group MT and group MC were less than group PT. In the aberrations, group MT had more elbows than those of group MC and group PT. The binding of group MC was least and group MT was less than group PT (P < 0.05). Under the condition of this study, crown-down technique using Mtwo rotary file is better and safer method than single length technique recommended by the manufacturer.

A Study on Operation Strategy by Multi-variate Regression of Deagu Arboretum Visitor's Satisfaction (대구수목원 이용객 만족모델을 통한 운영 방안 연구)

  • Kang, Kee-Rae
    • Journal of Korean Society of Forest Science
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    • v.101 no.1
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    • pp.36-45
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    • 2012
  • Education on the environment and plants offered by arboretum for today's people not only contribute to foster a better natural environment in urban region but also provide visitors with decent refreshment environment and beyond. In the study, the author undertook the observation on usage behavior and satisfaction model of arboretum visitors expect and investigated the facilities and programs to be offered by arboretum in order to propose the opinion regarding the service. For observation size of variables in a multiple regression analysis of variables is influencing satisfaction rankings walks the line of flow, the educational effect on the environment, cleanliness of the facility, visits pay, natural beauty, diversity of trees, accessibility and friendliness of staff, expansion of facilities in the arboretum and appeared as a complement. In case of visitor attribute, the residents living near the facility showed the highest visit frequency of more than 5 times, especially as part of taking a walk. This proves that the visit to arboretum is considered as part of everyday life, and thus a new program and walk path as well as movement route are needed to be developed for the visitors. In the question relating to the facilities and operation programs in Daegu Arboretum, particularly the requests by visitors, they responded that the establishment of cultural event, beautiful natural scenery, refreshment and convenience facilities is the most critical issue. In addition, the management on withered trees and bare lands is an urgent issue as well. In this sense, the Operation and Management Strategies based upon the visitor behaviors and model of satisfaction are needed to deal with the adoption of diverse events and festivals joined by local residents, ombudsman program, environmental program development for students and teachers within the region, negligent bare lands and withered tree replacement, and cafeteria facility improvement and supplement as well as the bench marking of other facilities than arboretums located in other regions. These items are thought to be sufficiently dealt with by Daegu Arboretum having no more external resources. It is recognized that the visitor satisfaction begins from a minor thing, and a small difference determines a great satisfaction, and thus the software approach rather than hardware one is in need.

The Effects of Social Class on the Leisure Activities in Korea: based on types and satisfaction of leisure activities (사회계층 변수에 따른 여가 격차 : 여가 유형과 여가 및 삶의 만족도를 중심으로)

  • Nam, Eun-Young;Choi, Yu-Jung
    • Korea journal of population studies
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    • v.31 no.3
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    • pp.57-84
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    • 2008
  • This study investigates the patterns of leisure in Korea and the effects of social class on the objective and subjective dimension of leisure activities and life satisfaction. A data set of 1376 Korean men and women over 18 years old is analyzed to yield five main results. First, Korean prefers domestic entertainment to outdoor activities as is exemplified by domestic audio-visual entertainment(TV/DVD/VCR) which ranks the highest in the favored leisure activity. Leisure activities are divided into four types; "activity-based", "relationship-based", "alcohol-based", "relaxation". Second, the function of leisure activity is to strengthen relationships. The main purpose of leisure activity is to relax and revitalize, while creating prospective social network ranks next to relax. But the effect of leisure time is often compromised by recurring thoughts related to work. Third, respondents with high educational and economic backgrounds are more likely to engage in "relationship-based," "activity-based", "alcohol-based" leisure type. However, such factors do not influence on "relaxation" type of leisure. While students and housewives rank highest in number of respondents, respondents with managerial/professional or white-collar/semi-professional occupations enjoy more diverse activities. Fourth, the effort to discern the significance of social class with respect to the leisure-activity-index revealed followings; the index scores elevate with higher education, younger age and higher income. Fifth, leisure-activity-index is the most important variable predicting leisure satisfaction. Leisure satisfaction is influenced by gender, age, income and occupation. The younger the age and higher the income, the higher it is the leisure satisfaction. Men are more satisfied with leisure activities than women. Students experience the highest satisfaction with leisure activities while service/sales workers, industrial/technical/blue-collar workers shows the least satisfaction. Also, the number of family members decreases significantly the leisure satisfaction. While "activity-based" leisure induces the highest satisfaction, "alcohol-based" leisure produces the least satisfaction. The frequency and diversity of leisure activities, and "activity-based" leisure incur the most positive effects on the life satisfaction.

Effect of Magnesium Administration on Preventing Arrhythmias after Coronary Artery Bypass Graft (관상동맥 우회술 후 마그네슘 투여가 심 부정맥을 예방하는 효과)

  • Kim, Jun-Hyun;Song, Hyun;Kim, Yong-Hee;Lee, Eun-Sang;Lee, Jay-Won;Song, Myung-Kun
    • Journal of Chest Surgery
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    • v.31 no.4
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    • pp.339-345
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    • 1998
  • Arrhythmias are common after cardiac surgery and are multifactorial. Intravenous magnesium administration reduces the frequency of ventricular arrhythmias in patient with symptomatic heart failure or acute myocardial infarction. This study was designed to evaluate the role of magnesium in preventing PVCs(premature ventricular contractions) occurred frequently after coronary artery bypass graft(CABG). 50 consecutive patients were prospectively entered into a randomized trial to determine the efficacy of postoperative magnesium therapy on the incidence of cardiac arrhythmias after elective coronary artery bypass graft. The patients underwent coronary angiography, echocardiography, electrocardiography and clinical laboratory study preoperatively. Continuous electrocardiographic monitoring was done and magnesium level was checked 0, 3, 6, 12, 18, 24, 36, 48, 60 and 72 hours postoperatively. Study group of 25 patients were given 4g of magnesium continuously over the first 24 hours and then 2g/24hours from 25 to 72 hours. The clinical characteristics of both groups were similar(p<0.05). The preoperative mean serum magnesium concentration was similar in both study group, 1.59mg/dl and control group, 1.71mg/dl. The mean postoperative serum magnesium concentration in study group elevated significantly over postoperative 12hours through 36hours(p<0.05). The postoperative mean serum magnesium concentration in control group declined and remained significantly depressed over immediate postoperation through 72hours. The mean serum magnesium concentration was significantly greater in the study group compared with the control group over postoperative 3hours through 72hours(p<0.05). There was a significant decrease in the incidence of arrhythmias such as PVCs(p<0.01) which might jeopardize hemodynamics. There were no recognized adverse effects of magnesium Administration. In conclusion, prophylactic magnesium administration seems to lessen the incidence and severity of rrhythmias after coponary artery bypass graft.

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Effects of Growth Regulators, Sucrose and Gelling Agents on Callus Growth and Plant Regeneration in Angelica koreana MAX. (강활(羌活)의 캘러스 증식(增殖) 및 식물체(植物體) 재분화(再分化)에 미치는 생장(生長) 조절제(調節劑), sucrose 및 배지(培地) 응고제(凝固劑)의 영향(影響))

  • Lee, Joong-Ho;Lee, Seung-Yeob;Namkoong, Seung-Bak
    • Korean Journal of Medicinal Crop Science
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    • v.4 no.1
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    • pp.78-85
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    • 1996
  • The effects of growth regulators, sucrose and gelling agents were investigated to increase the efficiency of the callus growth and plant regenerarion in tissue culture of Angelica koreana Max. The fresh weight and dry weight of subcultured callus was highest in MS medium supplemented with 1 mg/l 2,4-D. Callus growth was excellent in 2% sucrose, but it was inhibited in propotion to sucrose content. Effect of gelling agents on callus growth was highest on 1.2% agar and 0.4% Gelrite medium, respectively. The browning of callus was protected on the media supplemented with 10 mg/l ABA and 5 or 10 mg/l $AgNO_3$. In the callus induction and growth from the peduncle of immature inflorescence, 2,4-D was more effective than NAA, and the frequency of callus induction was highest as 81.7% in 2 mg/l 2,4-D. Plant was not regenerated from the callus derived from young leaf. Somatic embryos were developed from the surface of callus drived from the peduncle of immature inflorescence in the medium containing 0.5 mg/l 2,4-D, 1 mg/l kinetin, 5 mg/l ABA and 5 mg/l $AgNO_3$. Plants were developed from the matured somatic embryos in the medium supplemented with 0.2 mg/l 2,4-D and 1 mg/l kinetin.

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The Effect of Radiation Therapy on hemophilic Athropathy (혈우병성 관절증에 대한 방사선 치료의 역할)

  • Kang Jin Oh;Hong Seong Eon;Kim Sang Gi;Shin Dong Oh
    • Radiation Oncology Journal
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    • v.23 no.2
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    • pp.106-110
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    • 2005
  • Purpose : Repetitive bleeding into the joint space is the cause of debilitative hemophilic arthropathy. To interrupt this process, we treated the hemophilic patients suffering from repetitive joint bleeding with radiation therapy. Materials and Methods : From 1997 to 2001, a total of 41 joints from 37 hemophilic arthropathy patients were treated with radiation therapy at Kyung Hee University Hospital. The treated joints were 35 ankles, 3 knees and 3 elbows, respectively. The age of the patients ranged from 4 to 27 years (median age: 11 years). The radiation dose ranged from 900 cGy to 2360 cGy (median dose: 900cGy). The fraction size was 150 cGy, 180 cGy or 200 cGy. The number of bleeding in one year before and after radiotherapy was compared. Results : There was a tendency of frequent bleeding for the Patients younger than 11 (p=0.051) but there was also a tendency for more improvement in this group (p=0.057). The number of joint bleedings was related with joint pain (p=0.012) and joint swelling (p=0.033) but not with the Arbold-Hilgartner stage (p=0.739), cartilage destruction (p=0.718) and synovial hypertrophy (p=0.079). The number of bleeding was reduced in thirty-three cases, and eight cases showed no improvement after radiation therapy. The average number of bleeding in a month was 2.52 before radiotherapy, but this was reduced to 1.4 after radiotherapy (p=0.017). Conclusion : Radiation therapy was effective for the hemophilia patients with repetitive joint bleeding to decrease the bleeding frequency and to prevent hemophilic arthropathy.

Comparison of Raw versus Relative scores in the Assessment of Coping Patterns in Chronic Arthritis Patients (만성관절염 환자의 대응양상정도와 관련변수 분석 -원점수와 상대점수를 이용한 비교-)

  • Chun, Chung-Ja;Mun, Mi-Suk
    • Journal of muscle and joint health
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    • v.3 no.1
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    • pp.90-103
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    • 1996
  • The purpose of this paper is to compare two approach to assessment of coping patterns. The sampling method was a purposive sampling technique. The study participants were out patients 113 of rheumatoid arthritis center of one University hospitals in Seoul. Datas were collected from Nov. 13 to Nov. 24, 1995. The instruments used for this study were Graphic Rating Scales of pain, The Beck Depression Inventory and Coping Patterns tool. The collected data were analyzed for frequency, means, SD, factor analysis., Pearsons' correlations and ANOVA. The results were summerized as follows ; 1. When raw scores were used : there were not correlation in all three coping patterns. 2. When relative scores were used : there were significantly correlated in all three coping patterns. 1) Active coping and Positive-cognitive coping (r=-0.352, p< 0.0001) 2) Positive-cognitive coping and Negative-cognitive coping (r=-0.594, p< 0.0001) 3) Active coping and Negative-cognitive coping(r=-0.544, p< 0.0001) The results of this research with relative scales provided more insight into the correlation in all three coping patterns. 3. Pearsons' Correlations were computed for each coping pattern, age, pain level, duration of pain and BDI. 1) Using raw score : (1) Active coping was significantly related to pain level(sensory score ; r=0.268, p<0.05, affective score ; r=0.266, p< 0.05) (2) Positive-cognitive coping was significantly related to age (r=-0.252, p< 0.05), pain level (sensory score ; r= -0.244, p< 0.05) (3) Negative-cognitive coping was significantly related to depression level (r=0.312 p< 0.0001). 2) Using relative score (1) Active coping was significantly related to pain level(sensory score ; r=0.299, p<0.05, affective score ; r=0.246, p< 0.05) (2) Positive-cognitive coping was significantly related to age (r= -0.187, P< 0.05), pain level (sensory score ; r=-0.317, p<0.0001, affective score : r=-0.305, p<0.0001) and depression level(-0.339, p<0.0001)) (3) Negative-cognitive coping was significantly related to depression. level(r=0.313, p<0.0001). 4. When raw and realtive coping scores were compared to those of age groups, religious groups and BDI level(high, middle, low) ; 1) Using raw score : (1) Active coping : there were not significantly difference (2) Positive-cognitive coping ; 20-39 age group and 50-59age group had significantly higher scores than over 60age group. BDI-low level group had significantly higher scores than other groups. (3) Negative-cognitive coping : 20-39age group and over 60age group had significantly higher scores than 40-49age group. Non-religious group had significantly higher scores than christian group. BDI-high level group had significantly higher scores than other groups. 2) Using relative score : (1) Active coping : over 60 age group had significantly higher scores than 20-39 age group and 40-49age group had significantly higher scores than 20-39 age group (2) Positive-cognitive coping ; 40-49age group, 20-39age group and 50-59age group had significantly higher scores than over 60age group. Christian group had significantly higher scores than non-religious group. BDI-low level group had significantly higher scores than other groups. (3) Negative-cognitive coping ; Non-religious group had significantly higher scores than christian group and buddhistic group. BDI-high level group had significantly higher scores than other groups. The current data suggest that relative scores may yield a different perspective on coping patters than raw scores. The use of relative scores reveals the relation clearly, without its being blurred statistically by the effect of other coping strategies or being relegated to a partial correlation. The use of relative scores holds promise for delineating the relations between ways of coping and health-related behavior.

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