• 제목/요약/키워드: freezing condition

검색결과 303건 처리시간 0.025초

포화 사질토 뒷채움재의 비배수 동결에 의한 매설 강관의 거동 - 실대형 모형실험 연구 - (Effects of Freezing a Backfill Material under Undrained Condition on a Buried Pipe)

  • 강재모;이장근;김학승;이상윤;유병현;조남준
    • 한국지반공학회논문집
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    • 제30권8호
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    • pp.39-47
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    • 2014
  • 동절기에 지반에 매설된 상수도관은 지반의 동결 융해로 인해 과대한 지반 응력을 받게 되고, 이러한 현상이 겨울철 상수도관의 파괴 또는 변형에 많은 영향을 끼친다. 본 논문에서는 실 대형 모형실험을 통해 외부 온도 변화에 따른 매설관 주변 지반 내 온도거동과 매설관의 변위 및 응력거동을 확인하였으며, 지반이 동결됨에 따라 매설관 주변에 응력이 집중되고 동상이 발생하는 것을 확인하였다. 또한, 실험에 사용된 시료가 비동상성 시료인 사질토임에도 불구하고 함수비 조건과 배수조건에 따라 동상현상이 나타나는 것을 발견하였으며, 본 논문을 통해 매설관의 배딩(bedding)과 뒷채움재를 비동상 시료로 치환하더라도, 주변 지반의 배수상태, 지하수위, 함수비 등에 따라 동상에 대한 별도의 대책을 세워야 함을 제시하고자 하였다.

멸균방법에 따른 탈회동종골의 세포독성 여부에 관한 실험적 연구 (IN VITRO STUDY ON THE CYTOTOXICITY OF THE DIFFERENTLY STERILIZED DEMINERALIZED BONE POWDER)

  • 우기선;임창준;김세원;김종여
    • Maxillofacial Plastic and Reconstructive Surgery
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    • 제19권3호
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    • pp.287-299
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    • 1997
  • Procurement, cutting, cleansing, freezing, freeze-drying, and demineralization of the allogeneic bone must be made under the germ-free stable condition without bacterial and/or viral contamination. Even thought the bone is procured under the germ free condition, we must have confidence on disinfection of all the solutions that come in contact with tissue during the whole procedure. Lots of antibacterial agents have been introduced for chemical sterilization. Recently ethylene oxide gas sterilization or radiation sterilization is frequently selected as a secendary sterilization procedure. The biological and biochemical response of the graft material differs with the type and concentration of the sterilizing agents, and various toxic reactions have been reported due to the graft material itself and the substance released by the chemicals. The authors conducted the Millipore filter test to observe the toxic effect on L929 fibroblasts according to the effect on activity of succinate dehydrogenase, during the secondary sterilization of the demineralized allogeneic bone powder with irradiation or ethylene oxide gas. The result were as follows : 1. Around the copper disk, positive control group, 10mm diameter discoloration was observed. 2. As same as the negative control group, the disk showed no discoloration. 3. The demineralized allogeneic bone which was sterilized with ethylene oxide gas or irradiation showed no cytotoxicity. 4. From this results, it is suggested that treatment with ethylene oxide gas or irradiation should be effective to sterilize the deminineralized allogeneic bone.

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도로 위험 상황의 전송 방법 (Information Transfer Method of Dangerous Road Condition)

  • 안수진;김영욱;한민홍
    • 한국컴퓨터정보학회논문지
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    • 제12권1호
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    • pp.189-197
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    • 2007
  • 고속도로 상을 운전하는 운전자가 사고, 낙물, 또는 결빙 등 도로위험상황을 발견한 즉시 후속차량에게 이 정보를 전달함으로써 사고를 미연에 방지하는 안전시스템을 개발하였다. 이를 위해서 각 차량은 GPS와 송수신 단말기로 구성된 OBU를 장착하고 지난 일정한 거리의 도로 궤적을 항상 갱신하고 저장하여 사고 위험사황을 목격 시 버튼을 눌러 사고위험유형과 궤적정보가 송신한다. 정보를 받은 모든 차량은 자차의 위치나 방향이 수신된 궤적정보 상에 있는지를 판단하고 일정한 거리내의 후속차량만이 이에 반응하여 경보메시지를 발생시키게 된다. 447Mhz의 주파수의 트랜스시버를 갖는 OBU를 장착한 두 대의 실험차량을 약 120m의 간격을 두고 $30km/h{\sim}50km/h$의 속도로 도로주행 실험을 한 결과 문제없이 통신이 이루어짐을 확인하였다. 따라서 본 시스템은 운전자가 고속도로상의 위험 상황을 사전에 인식하고 대처할 수 있게 하므로 안전 주행의 도구로 사용되어 질 수 있을 것이다.

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Post-COVID-19 pain syndrome: a descriptive study in Turkish population

  • Topal, Ilknur;Ozcelik, Necdet;Atayoglu, Ali Timucin
    • The Korean Journal of Pain
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    • 제35권4호
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    • pp.468-474
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    • 2022
  • Background: The new type of corona virus has a wide range of symptoms. Some people who have COVID-19 can experience long-term effects from their infection, known as post-COVID conditions. The authors aimed to investigate prolonged musculoskeletal pain as a symptom of the post-COVID-19 condition. Methods: This is a descriptive study on the patients who were diagnosed with COVID-19 in a university hospital, between March 2020 and March 2021. Patient records and an extensive questionnaire were used to obtain relevant demographic and clinical characteristics, including hospitalization history, comorbidities, smoking history, duration of the pain, the area of pain, and the presence of accompanying neuropathic symptoms. Results: Of the diagnosed patients, 501 agreed to participate in the study. Among the participants, 318 had musculoskeletal pain during COVID-19 infection, and 69 of them reported prolonged pain symptoms as part of their a post-COVID condition which could not be attributed to any other cause. The mean duration of pain was 4.38 ± 1.73 months, and the mean pain level was 7.2 ± 4.3. Neuropathic pain symptoms such as burning sensation (n = 16, 23.2%), numbness (n = 15, 21.7%), tingling (n = 10, 14.5%), stinging (n = 4, 5.8%), freezing (n = 1, 1.4%) were accompanied in patients with prolonged musculoskeletal pain. Conclusions: Patients with COVID-19 may develop prolonged musculoskeletal pain. In some patients, neuropathic pain accompanies it. Awareness of prolonged post-COVID-19 pain is crucial for its early detection and management.

콘크리트궤도 하부 조립지반재료의 장기압축침하에 관한 연구 (Long-term Compression Settlement of Granular (Rock/Soil Mixture) Fill Materials under Concrete Track)

  • 이성진;이일화;이진욱;이준석
    • 한국지반공학회논문집
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    • 제25권8호
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    • pp.95-106
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    • 2009
  • 본 연구에서는 암석과 흙이 혼합된 성토재료를 대상으로 다짐 시 함수조건 및 입도조건 등에 따라 Wetting이 성토제체의 장기압축침하에 미치는 영향을 중심으로 검토하였다. 그 결과 완전 침수를 모사한 경우 침하량이 상대적으로 매우 크게 나타났으며, 강우침투와 같이 반복적인 함수비의 증가 시에도 반복적인 침하가 관측되었다. 반면 습윤상태에서 다져진 시료나 세립분이 없는 재료의 경우에는 Wetting에 의한 침하량이 상대적으로 적게 발생되는 것을 확인할 수 있었다. 또한 동결 융해가 반복되는 경우 입자파쇄의 가속화로 압축침하가 지속적으로 발생되고 있는 것을 확인할 수 있었다. 결론적으로 이와 같은 재료에서의 장기적인 성토체 압축침하거동에는 크리프의 영향보다 함수비 증가나 반복적인 동결융해 조건이 더 큰 영향을 미치고 있는 것으로 나타났다.

Determination of Optimal Storage Condition for Pre-packed Hanwoo Loin

  • Seol, Kuk-Hwan;Park, Tu San;Oh, Mi-Hwa;Park, Beom-Young;Cho, Seong In;Lee, Mooha
    • 한국축산식품학회지
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    • 제33권3호
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    • pp.390-394
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    • 2013
  • The aim of this study was to determine the optimal storage condition of pre-packed Hanwoo beef without freezing. Hanwoo loin was purchased from a local distributor at 48 h after slaughter, then sliced in $1.5{\pm}0.5$ cm thickness, and packed in a polyethylene (PE) tray covered with linear low-density polyethylene (LLDPE) film. The studied factors to set the optimal storage condition were chamber temperature (5, 2.5 and $-1^{\circ}C$ for 14 d), cooling method (direct and indirect cooling system), and ultraviolet (UV) light irradiation for beef surface sterilization (0, 30, 60, and 120 min). The changes of pH, thiobarbituric acid reactive substances (TBARS) and number of aerobic bacteria were measured during storage. Beef samples stored in $-1^{\circ}C$ showed the minimal increasing rate in TBARS and microbial growth. After 15 d of storage, there was no significant difference in pH and TBARS values. However, the microbial population of beef stored in direct type cooling chamber ($4.25{\pm}0.66$ Log CFU/g) was significantly lower than that of beef stored in indirect type chamber ($6.47{\pm}0.08$ Log CFU/g) (p<0.05). After 4 d of storage, 60 or 120 min UV light irradiated beef samples showed significantly lower microbial population, and at 14 d of storage, 60 min UV irradiated beef sample showed significantly lower microbial population ($3.14{\pm}0.43$ Log CFU/g) than control ($4.46{\pm}0.13$ Log CFU/g) (p<0.05). However, TBARS values of 60 or 120 min UV light irradiated beef samples were significantly higher than non-irradiated beef sample after 4 d of storage (p<0.05).

실물부재시험에서의 양생방법 차이에 따른 한중콘크리트외 온도이력 및 강도특성에 관한 연구 (A Study on the Strength Properties and the Temperature Curve of Winter Concrete According to the Difference of Curing Method in Mock-up Test)

  • 김영진;이상수;원철;박상준
    • 콘크리트학회논문집
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    • 제15권4호
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    • pp.541-548
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    • 2003
  • 본 연구는 내한촉진제를 사용한 콘크리트의 현장 적용성을 알아보기 위한 기초적인 실험으로서, 실물부재시험을 통해 각종 양생방법에 따른 온도이력 및 강도특성을 검토하고 한중콘크리트에서 실구조물과 동일한 강도이력을 가질 수 있도록 강도관리용 공시체의 적정 양생방법을 평가하기 위한 것이다. 실물부재시험에서 양생방법에 따른 온도이력은 일반콘크리트(단열양생), 일반콘크리트(급열양생), 내한촉진제 콘크리트 및 일반콘크리트(양생막 양생) 순으로 크게 저하하는 경향을 보이고 있었다. 강도특성은 양생조건이 열악한 양생막 내 외부에서의 일반콘크리트 공시체의 경우에는 초기동해에 따른 강도발현 저하를 보인 반면에 내한촉진제 콘크리트의 경우에는 재령이 경과할수록 계속적인 강도발현을 보이고 있어 내한촉진제의 사용에 따른 동결온도 저하효과 및 저온환경하에서의 경화촉진효과를 나타내고 있었다. 한중콘크리트의 강도관리용 공시체의 적정 양생방법을 검토한 결과, 부재 내부에 매립하여 양생한 경우 및 간이단열양생을 실시한 경우에 있어서의 구조체 콘크리트와의 온도이력은 다소 차이를 나타내고 있지만, 코어강도와는 비교적 근사하게 나타나고 있어 구조체 강도평가를 위한 이들 양생방법의 유효성을 확인할 수 있었다.

핵이식 수정란의 동결, 융해 및 이식에 의한 클론동물의 생산 II (Production of cloning animals by fresh and frozen-thawed nuclear transfer embryos II)

  • 황우석;조충호;이창우;이병천
    • 대한수의학회지
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    • 제33권3호
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    • pp.547-554
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    • 1993
  • This study was carried out to investigate the best condition for in vitro and in vivo culture after freezing and thawing of nuclear transplant 2 cell embryos. When nuclear transplant embryos were submitted to electrofusion, the significantly higher fusion rates of 2 cell donor nuclei were achieved at the electric field strength of DC 1.5 kV/cm for 100 and $150{\mu}sec$, DC 2.0 kV/cm for 100 and $150{\mu}sec$ than DC 1.0 kV/cm for 100 and $150{\mu}sec$(p<0.01). The significantly higher fusion rates of 4 cell donor nuclei were achievecl at DC 2.0 kV/cm for 100 and $150{\mu}sec$ than DC 1.0 kV/cm for 100 and $150{\mu}sec$(p<0.01). The fusion rates in 8 cell donor nuclei were 94.2~99.3%. The developmental potency to blastocyst in 2 cell donor nuclei was significantly higher in DC 2.0 kV/cm for $150{\mu}sec$ treated group(p<0.01). The significantly higher developmental potency to blastocyst in 4 cell donor nuclei were achieved at the electric field strength of DC 2.0 kV/cm for $150{\mu}sec$ than DC 1.5 kV/cm for 100 and $150{\mu}sec$, DC 2.0 kV/cm for $100{\mu}sec$ treated group(p<0.01). The develop mental potency to blastocyst in 8 cell donor nuclei was significantly higher in DC 2.0 kV/cm for $100{\mu}sec$ treated group(p<0.01). The developmental potency to blastocyst after nuclear transplantation was significantly higher in 2 cell donor nuclei than in 8 cell donor nuclei(p<0.01). When the recovered embryos in normal morphology were cultured in vitro, there were no significant differences in the developmental potency to blastocyst between the freezing methods and the concentrations of cryoprotectant(p<0.01). The production rates of offspring after transfer of nuclear transplant embryos to recipient mouse were no significant difference in 2, 4 and 8 cell donor nuclei.

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체외수정 및 미세조작에 의한 가축(胚)의 생산과 효율적 이용에 관한 연구 II. 소 체외수정 난포란의 발생단계별 동결가 이식후의 생존성 (Studies on Production and Efficient Utilization of Livestock Embryos by In Vitro Fertilization and Micromainipulation II. Effects of Embryonic Development on Survival after Freezing Transfer in Bovine Oocytes Fertilized In Vitro)

  • 정영채;김창근;윤종택;최선호;정광조
    • 한국가축번식학회지
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    • 제17권3호
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    • pp.233-242
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    • 1993
  • The effects of in vitro maturation and sperm treatment condition on the in vitro fertilization (IVF) and developmental capacity of bovine oocytes were investigated and the development of embryos was compared under the 2 different co-culture system, with GC or BOEC. The cultured embryo to 16 cell or morula wre transferred into recipients or frozen by 2 different freezing method. The results obtained were summarized as follows; 1. In vitro maturation rates of vovine follicular oocytes cultrued in TCM199 with 10% FCS or ECS were 64.0% and 72.7%, but the case of addition of 10% FCS or ECS to TCM199 co-cultured with granulosa cells were 81.3% and 84.0%, respectively. IVM rate of three TCM199 added to granulosa cells was higher than that of media without granulosa cells. 2. When bovine follicular oocytes were matured in TCM199 with 10% FCS and GC and then fertilized in vitro by sperm treated with caffeine, embryo developments of bovine oocytes co-cultured with BOEC were 38.4% and 51.4%, respectively. But those of bovine oocytes co-cultured with GC were 52.2% by sperm treated with caffeine-heparin. 3. Cleavage rates of bovine oocytes cultured with 10% FCS alone and fertilized in vitro by sperm treated with caffeine-heparin was 33.0%. 4. When bovine follicular oocytes were matured in TCM199 with 10% FCS and GC, embryo developments of bovine ooctyes co-cultured with BOEC of GC were 46.0% and 50.2%, respectively. 5. When bovine follicular oocytes were matured in TCM199 with 10% ECS and GC, embryo developments co-cultured with BOEC or GC were 45.2% and 51.4%, respectively. 6. When Korean Native cow's follicular oocytes matured in TCM199 with 10% FCS and GC, embryo developed co-cultured with BOEC or GC were 45.2% and 51.4%, respectively. 6. When Korean Native cow's follicular oocytes matured in TCM199 with 10% FCS and GC, embryo developments of the bovine oocyte co-cultured with BOEC and GC were 41.8% and 60.1%. But with FCS 10% those of the bovine oocytes co-cultured with BOEC and GC were 42.0% and 48.4%, respectively. 7. When Holstein's follicular oocytes were matured in TCM199 with 10% ECS and GC, embryo developments fo the bovine oocytes co-cultured with BOEC and GC were 50.0% and 57.7%, but with ECS 10% those of the bovine oocytes co-cultured with BOEC and GC were 52.2% and 56.5%, respectively. 8. The viability of frozen-thawed embryos ranged from 60~80% and those of frozen-thawed embryos from vitrification was lower than that from conventional metiod. 9. The selected fresh embryos were transferred nonsurgically to 7 recipients but did not result in pregnancy.

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빙결점동결에 의한 수산식품의 품질보존에 관한 연구 1. 빙결점동결 저장 중의 구운 고등어의 품질변화 (Partial Freezing as a Means of Keeping Quality of Sea Foods 1. Keeping Quality of Baked Mackerel Muscle during Partially Frozen Storage)

  • 이응호;김정균;하재호;오광수;차용준
    • 한국식품영양과학회지
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    • 제12권2호
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    • pp.62-65
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    • 1983
  • 어육을 단기간 저장하는데 효율적인 저장방법으로 알려진 빙결점동결법을 조리식품에 적용하여 저장중의 품질안정성에 대하여 실험한 결과를 요약하면 다음과 같다. 빙결점동결법으로 저장한 구운 고등어는 지방의 산패면에서 $5^{\circ}C$$-20^{\circ}C$에서 저장한 것보다 유도기가 약간 연장되는 효과가 있었으며, pH와 VBN는 세가지 저장조건에 따른 큰 차이가 없었다. 저장중 pH는 약간 감소하는 반면 휘발성염기질소는 저장초기에 급증하고 저장 4일 이후에는 큰 변화가 없었다. 생균수는 빙결점동결법으로 저장한 것이 $5^{\circ}C$ 저장한 것보다 상당히 낮은 값을 나타내었으며, $-20^{\circ}C$에서 저장한 것과 비교하여 큰 차이가 없었다. 그리고 관능검사의 결과도 빙결점동결법으로 저장한 것은 $-20^{\circ}C$에서 저장한 것과 비교하여 큰 손색이 없었다.

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