• 한국어병학회지
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• 제22권2호
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• pp.167-172
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• 2009

본 연구에서는 넙치 혈청의 보존 조건이 ELISA 결과에 미치는 영향을 확인하기 위하여, 항 BSA 넙치 혈청을 이용해 보존 온도, 보존 기간 및 해동 횟수에 따른 ELISA값을 비교하였다. 넙치 혈청을 보존 온도 (-80, -20, 4 및 20${^{\circ}C}$) 및 기간 (1, 30, 69 및 124일)에 따른 ELISA OD 값을 비교한 결과, 모든 시료에서 뚜렷한 OD값의 차이는 확인되지 않았다. -80℃ 및 -20${^{\circ}C}$에 보존된 혈청을 사용하여 해동횟수 (1, 5, 10회)에 따른 ELISA OD값을 비교한 결과, 해동을 반복한 혈청 및 미동결 혈청의 OD값은 뚜렷한 차이를 보이지 않았다. 이상의 결과로 넙치 혈청은 -80, -20, 4 및 20${^{\circ}C}$에서 124일간 보존하거나 혈청을 10회 반복 해동하여도 ELISA 결과에 영향을 끼치지 않는 것이 확인되었다.

• 한국산학기술학회논문지
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• 제19권2호
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• pp.623-631
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• 2018

공용중인 콘크리트 구조물의 내구성 연구는 한 가지 원인에 대한 단독열화 연구를 주로 연구하였지만 실제 구조물의 공용시기에는 두 가지 이상의 환경에 노출되어 있어 이러한 복합열화에 대한 관심이 최근 증가하고 있는 실정이다. 복합열화에 대해 일반적으로 염해와 동결융해에 대해서는 일부 연구가 진행되어 있지만 콘크리트의 양생 직후부터 발생하는 탄산화와 다른 내구성능저하에 대한 복합열화 연구는 매우 미흡한 실정이다. 특히, 탄산화를 중심으로 하는 복합열화는 탄산화의 경우 탄산화 진행시 콘크리트 조직이 치밀해진다는 문헌고찰에 따라 조직의 치밀해지는 이유로 연구가 미흡하다. 본 연구에서는 콘크리트의 탄산화 및 동결융해에 대한 복합열화를 평가 분석한 결과이다. 평가를 위하여 탄산화된 콘크리트를 동결융해 300싸이클까지 복합열화 시험을 실시하여 동탄성계수와 압축강도를 측정하였다. 그 결과 물-결합재비에 대한 영향 평가에서 35 %의 콘크리트가 55 %의 콘크리트에 비해 복합열화 성능이 낮은 것을 확인하였고, 광물질혼화재료를 사용한 경우 플라이애시와 고로슬래그미분말을 사용한 경우 동일 물-결합재비에서 보통포틀랜드 시멘트 사용 콘크리트보다 복합열화에 대한 성능이 개선되는 것으로 나타났다.

• 한국도로학회논문집
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• 제19권6호
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• pp.129-137
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• 2017

PURPOSES : The purpose of this study is to develop bridge deck concrete materials based on ordinary Portland cement concrete, and to evaluate the applicability of the developed materials through material properties tests. METHODS : For field implementation, raw material (cement, fine aggregate, and coarse aggregate) properties, fresh concrete properties (slump and air content), strength (compressive, flexural and bond strength) gain, and durability (freeze-thaw resistance, scaling resistance, and rapid chloride penetrating resistance) performance were evaluated in the laboratory. RESULTS : For the selected binder content of $410kg/m^3$, W/B = 0.42, and S/a = 0.48, the following material performance results were obtained. Considering the capacity of the deck finisher, a minimum slump of 150 mm was required. At least 6 % of air content was obtained to resist freeze-thaw damage. In terms of strength, 51.28 MPa of compressive strength, 7.41 MPa of flexural strength, and 2.56 MPa of bond strength at 28 days after construction were obtained. A total of 94.9 % of the relative dynamic modulus of elasticity after 300 cycles of freeze-thaw resistance testing and $0.0056kg/m^2$ of weight loss in a scaling resistance test were measured. However, in a chloride ion penetration resistance test, the result of 3,356 Coulomb, which exceeds the threshold value of the standard specification (1000 Coulomb at 56 days) was observed. CONCLUSIONS : Instead of using high-performance modified bridge deck materials such as latex or silica fume, we developed an optimum mix design based on ordinary Portland cement concrete. A test construction was carried out at ramp bridge B (bridge length = 111 m) in Gim Jai City. Immediately after the concrete was poured, the curing compound was applied, and then wet mat curing was applied for 28 days. Considering the fact that cracks did not occur during the monitoring period, the applicability of the developed material is considered to be high.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2000년도 봄 학술발표회 논문집
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• pp.489-495
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• 2000

When microorganisms such as bacteria and fungi are injected into porous medium such as soils along with appropriate substrate and nutrients, biomass retained in the soil pore. Soil pore size and shape are varied from the initial condition as a result of biofilm formation, which make hydraulic conductivity reduced. In this research, hydraulic conductivity reduction was measured after microorganism are inoculated and cultured with synthetic substrates and nutrients. Biomass-soil mixture was evaluated its applicability to the field condition as an alternative liner material in landfill by measuring hydraulic conductivity change after repetitive freeze-thaw cycles.

• 지질공학
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• 제25권2호
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• pp.165-178
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• 2015

Physical and chemical weathering degrades rock, affecting its structural properties and thus the stability of stone buildings or other structures. Confocal laser scan microscopy (CLSM) is used here to observe temporal changes in the surface roughness of rock samples under simulated accelerated weathering. Samples were pressurized to 50, 55, or 70 MPa using a pressure frame, and subjected to freeze/thaw cycling controlled by a thermostat. The temperature was cycled from -20℃ to 40℃ and back. After each 20 cycles, CLSM was used to assess the change in surface roughness, and roughness factors were calculated to quantify the progression of the surface condition over time. Variations in cross-section line-roughness parameters and surface-roughness parameters were analyzed for specific parts of the sample surfaces at 5× and 50× magnification. The result reveals that the highest and lowest values of the roughness factors are changed according to elapsed time. Freezing/thawing at high pressure caused larger changes in the roughness factor than at low pressure.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2008년도 춘계 학술발표회 제20권1호
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• pp.161-164
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• 2008

구조물은 사용연한 경과에 따른 노후화 및 용도 변경 등에 의한 보수.보강시 널리 행해지는 부착공법 중 탄소섬유, 아라미드섬유, 유리섬유 등을 이용한 섬유보강 폴리머(Fiber-reinforced polymer,FRP) 보강공법은 섬유 자체의 내화학성, 내구성과 우수한 시공성 등의 장점을 지니고 있다. 현재 FRP 부착공법을 이용한 보강설계 및 보강구조물의 내력 계산시, 콘크리트 부재와의 부착력을 완전부착으로 가정하고 있으나 콘크리트 구조물이 내부적 요인 및 외부 환경요인에 의해 열화될 경우 내력저하 및 1차적인 표면부 열화로 인한 FRP 부착면적의 손실발생이 우려된다. 하게 된다. 따라서 노후화에 따라 내력저하를 보이는 철근콘크리트 구조물에 FRP 보강시 열화에 따른 FRP 보강부재와 콘크리트 모재 간의 부착력 손실에 관한 연구가 필요하며, 본 연구에서는 환경적 요인에 의해 열화되는 철근콘크리트 부재의 파괴양상 및 구조성능과 FRP 보강부재와 콘크리트 모재와의 부착성능 저하현상을 분석하기 위하여 동결융해에 따른 CFRP 쉬트의 보강성능을 실험적으로 규명하고자 한다.

• Structural Engineering and Mechanics
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• 제90권5호
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• pp.481-492
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• 2024

This research examined the mechanical behavior of fiber-reinforced concrete at unstable environmental conditions. Concrete composites with varying percentages of steel and glass fibers were analyzed. Compressive, indirect tensile, and fracture toughness properties were evaluated using the Edge Notched Disc Bend (ENDB) test under freezing-thawing and acidic environments and the results were compared with normal conditions. Steel fibers decreased the strength in the specified cycles, while glass fibers showed a normal strength trend. The compressive, tensile and fracture toughness of the samples containing 1.5 vol.% fibers showed a 1.28-, 2.13- and 4.5-fold enhancement compared to samples without fibers, after 300 freezing-thawing cycles, respectively.

• Journal of Microbiology
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• 제45권6호
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• pp.510-514
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• 2007

Twenty-five bacterial strains that secrete mucous materials were isolated from sediment obtained from King George Island, Antarctica. Seven of these strains proved capable of producing cryoprotective exopolysaccharides. The strain KOPRI 21653 was selected for the further study of an anti-ice-nucleating polysaccharide (ANP), which originated from a polar region. KOPRI 21653 was identified as Pseudoalteromonas arctica as the result of 16S rRNA analysis. The exopolysaccharide, P-21653, was purified completely from the KOPRI 21653 cell culture via column chromatography and protease treatment. The principal sugar components of P-21653 were determined to be galactose and glucose, at a ratio of 1:1.5, via GC-MS analysis. The cryoprotective activity of P-21653 was characterized via an E. coli viability test. In the presence of 0.1% (w/v) P-21653, the survival ratio of E. coli cells was as high as 82.6% over three repeated freeze-thaw cycles. The survival ratio decreased drastically to 71.5 and 48.1 %, respectively, in five and seven repeated cycle conditions; however, the survival ratios were greater over three (96.6-92.1%) to seven (100.5-91.6%) freeze-thaw cycles in the presence of 0.5 and 1.0% (w/v) P-21653. In addition, at much lower concentrations (0.1-1.0%), P-21653 resulted in survival ratios (83.1-98.4%) similar to those of two commercially available cryoprotectants ($V_{EG}$ plus X-1000, 92.9% and $V_{M3}$, 95.3%), which were utilized at the recommended concentrations (90%). The biochemical characteristics of exopolysaccharide P-21653 reflect that this compound may be developed as a useful cryoprotectant for use in medical applications and in the food industry.

• 한국축산식품학회지
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• 제34권6호
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• pp.742-748
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• 2014

This study was conducted to evaluate the activities of antioxidant enzyme (glutathione peroxidase (GSH-Px)) and lysosomal enzymes (alpha-glucopyranosidase (AGP) and beta-N-acetyl-glucosaminidase (BNAG)) of the longissimus dorsi (LD) muscle from Hanwoo (Korean cattle) in three freezing conditions. Following freezing at -20, -60, and $-196^{\circ}C$ (liquid nitrogen), LD samples (48 h post-slaughter) were treated as follows: 1) freezing for 14 d, 2) 1 to 4 freeze-thaw cycles (2 d of freezing in each cycle), and 3) refrigeration ($4^{\circ}C$) for 7 d after 7 d of freezing. The control was the fresh (non-frozen) LD. Freezing treatment at all temperatures significantly (p<0.05) increased the activities of GSH-Px, AGP, and BNAG. The $-196^{\circ}C$ freezing had similar effects to the $-20^{\circ}C$ and $-60^{\circ}C$ freezing. Higher (p<0.05) enzymes activities were sustained in frozen LD even after 4 freeze-thaw cycles and even for 7 d of refrigeration after freezing. These findings suggest that freezing has remarkable effects on the activities of antioxidant enzyme and lysosomal enzymes of Hanwoo beef in any condition.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2000년도 가을 학술발표회논문집(I)
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• pp.433-438
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• 2000

The purpose of this study is to examine the resistance of water-permeable concretes to freezing and thawing action. The water-permeable concretes with cement-aggregate ratio of 1:5.5(by weight) and two kinds of admixture content [SP : superplasticizer(0, 1.0%), HPAE : high performance air entraining agent(0.5, 1%)] used OPC(ordinary portland cement) as binder were prepared, and then tested for relative dynamic modulus of elasiticity, mass change, length change and durablity factor. It's been concluded from the test results that the superior relative dynamic modulus of elasiticity and durability factor of water-permeable concretes were obtained at superplaciticizer 1.0% after 300 cycles. The water-permeable concretes used superplasiticizer 1.0% having relative durability factor of 88% after 300 cycles.