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Effect of storage conditions of olive flounder Paralichthys olivaceus serum on enzyme-linked immunosorbent assay  

Kim, Wi-Sik (Division of Food Science and Aqualife Medicine, Chonnam National University)
Jang, Min-Seok (Division of Food Science and Aqualife Medicine, Chonnam National University)
Kim, Jong-Oh (Division of Food Science and Aqualife Medicine, Chonnam National University)
Kim, Du-Woon (Division of Food Science and Aqualife Medicine, Chonnam National University)
Jung, Sung-Ju (Division of Food Science and Aqualife Medicine, Chonnam National University)
Kim, Seok-Ryel (Pathology division, National Fisheries Research and Development Institute)
Park, Myoung-Ae (Pathology division, National Fisheries Research and Development Institute)
Oh, Myung-Joo (Pathology division, National Fisheries Research and Development Institute)
Publication Information
Journal of fish pathology / v.22, no.2, 2009 , pp. 167-172 More about this Journal
Abstract
Antibody-detection enzyme-linked immunosorbent assay (ELISA) was performed to determine whether the absorbance value of ELISA is influenced by the different serum storage conditions of olive flounder Paralichthys olivaceus. Flounder antiserum to bovine serum albumin was stored at -80, -20, 4 and 20${^{\circ}C}$ for 1, 30, 69 and 124 days, respectively. In addition, the flounder antisera were frozen at -80 and -20${^{\circ}C}$, respectively and then repeated 1, 5 and 10 thaw-freeze cycles. No significant difference was shown in ELISA optical density (O.D.) values of sera, which were stored at the above mentioned storage conditions during 124 days. ELISA O.D. values of unfrozen serum samples, which were previously stored at 4${^{\circ}C}$, were almost similar to those of sera undergoing 1, 5, and 10 freeze-thaw cycles after stored at -80 or -20${^{\circ}C}$. In conclusion, the ELISA O.D. values of flounder sera were not affected by various storage conditions: different temperatures (-80, -20, 4 and 20${^{\circ}C}$), durations of storage (1, 30, 69 and 124 days), and repeated thaw-freeze cycles (1, 5, and 10 times).
Keywords
Antibody detection; Enzyme-linked immunosorbent assay; Anti-sera; Olive flounder; Storage;
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Times Cited By KSCI : 1  (Citation Analysis)
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