• 제목/요약/키워드: freeze substitution

검색결과 32건 처리시간 0.026초

급속동결(急速凍結)과 동결치환(凍結置換)에 의한 조직세포(組織細胞) 관찰법(觀察法) (Improved Method of Rapid Freezing and Freeze-Substitution for Tissue Cells)

  • 한성식;박규택
    • Applied Microscopy
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    • 제20권2호
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    • pp.1-11
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    • 1990
  • The ultrastructure of freeze-substituted Malpighian tubule cells of German cockroach after rapid freezing was compared to that fixed with conventional chemical fixation. As results, freeze-substitution proved to be the superior technique revealing much more structural details of the tissue cells. Especially, all kinds of membrane structures including infoldings, microfilament, microtubules and ribosomes were best preserved in this material.

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냉동 동결 건조 머큐베리 분말 첨가 파운드케이크의 항산화 활성과 품질 특성 (Antioxidant Activity and Properties Characteristics of Pound Cakes Prepare using Freeze Dried Maquiberry (Aristotelia chilensis [Mol.]) Powder)

  • 이혜정
    • 한국식품영양학회지
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    • 제27권6호
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    • pp.1067-1077
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    • 2014
  • A study was undertaken to examine the effect of adding freeze dried maquiberry powder on the quality of pound cakes. Freeze dried maquiberry was added to the flour at a ratio of 0.5, 1, 1.5 and 2.5%. The antioxidant activity was estimated by DPPH free radical scavenging activity, the total phenolic compounds content, and flavonoid content in freeze dried maquiberry powder of pounds cakes. Antioxidative activity was highly correlated with the total phenolic and total flavonoids contents of freeze dried maquiberry powder of pound cakes (r=0.9126, p<0.001, r=0.8366, p<0.001, respectively). The quality characteristics of freeze dried maquiberry powder of poundcakes were estimated. The specific volume decreased significantly with increased substitution level of freeze dried maquiberry powder (p<0.01). The lightness significantly decreased with increased freeze-dried maquiberry powder of pound cake crust and crumb (p<0.01, p<0.01). The hardness and adhesiveness increased, while gumminess tended to reduce with increased maquiberry powder. The consumer acceptability score of 0.5~2.5% freeze dried maquiberry powder of pound cakes ranked significantly (p<0.01) higher than those of the other groups in overall preference. These results showed that freeze dried maquiberry powder is a good ingredient to increase consumer acceptability and health.

Ultrastructural Studies of Encystment in Allomyces macrogynus

  • Kim, Jung-Soeup;Youn, Hyun-Joo;Cho, Chung-Won
    • Journal of Microbiology
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    • 제34권3호
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    • pp.220-224
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    • 1996
  • Ultrastructural organization of encysting zoospores of Allomyces macrogynus was examined using the methods of cryofixation and freeze substitution. During enxcystment, obvious changes were observed at the surface of the plasma membrane and in the structure of gamma particles. Many multivesicular bodies associated with the plasma membrane were observed at early stages of encystment. After induction of encystment, vesicles were found within the gamma particles. These vesicles appeared to leave gamma particles after forming multivesicular bodies. This study suggests that the cell wall formation during encystment is mediated by the fusion of multivesicular bodies with the plasma membrane.

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폴리프로필렌을 혼합한 재생골재콘크리트의 내구성에 관한 연구 (A Study on the Durability of Recycled Aggregate Using Polypropylene Fibers)

  • 라재웅;신재인;양승배;구봉근
    • 한국콘크리트학회:학술대회논문집
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    • 한국콘크리트학회 2000년도 가을 학술발표회논문집(I)
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    • pp.419-424
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    • 2000
  • The primary objectives of this study are to investigate the properties of strength and durability of recycled aggregate concrete was added polypropylene as variables and to fabricate fine concrete in some conditions. The variables are substitution ratios of recycled aggregate(0, 30, 50, 100%) and additions of polypropylene(0, 0.2, 0.5, 1.0%). Compressive strength test to investigate strength properties and freeze-thawing test and drying shrinkage test to durability properties were done. As the result of this study, When variables are substitution ratio(30%) of recycled aggregated and addition(0.5%) of polypropylene, fine concrete was fabricated.

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Durability properties of concrete containing metakaolin

  • Nas, Memduh;Kurbetci, Sirin
    • Advances in concrete construction
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    • 제6권2호
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    • pp.159-175
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    • 2018
  • The main aim of this study is to investigate the possible effects of metakaolin on strength and durability properties of concrete. For this purpose, concrete mixtures are produced by substituting cement with metakaolin 0, 5, 10 and 20% by weight. The amount of binder for the concrete mixtures are 300 and $400kg/m^3$ with a constant water to cement ratio of 0.6. Compressive and bending strengths, freeze-thaw and high-temperature resistances, capillary coefficients and rapid chloride permeability properties were determined and compared each other. Because of all the experiments conducted, it has been found that the use of metakaolin as a pozzolanic additive in concrete have positive effects especially on compressive and bending strengths, capillary, rapid chloride permeability, freeze-thaw resistance, and high temperatures, up to $800^{\circ}C$. The results indicated that the performance of concrete can be enhanced by metakaolin. Particularly, compressive strength and durability properties have found to be improved with increasing metakaolin content which is attributed to pozzolanic activity and filler effect. Furthermore, metakaolin has relatively positive impacts under elevated temperatures and freeze-thaw effects. However, almost all the strengths of entire concrete specimens are lost at $800^{\circ}C$. Consequently, the optimum metakaolin substitution ratio can be suggested to be 20% as per this study.

치환도가 초산 고구마전분의 물리적 특성에 미치는 영향 (Physical Properties of Acetylated Sweet Potato Starches as Affected by Degree of Substitution)

  • 유병승;이혜린
    • 한국식품영양과학회지
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    • 제40권7호
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    • pp.1048-1052
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    • 2011
  • 각기 다른 치환도를 가진 초산 고구마전분 페이스트의 유동특성 및 투과도 그리고, 이들 전분 겔의 냉.해동안정성 및 강도가 치환도의 함수로 분석되었다. 유동특성 측정 결과 power law 모델식과 Casson 모델식에 잘 적용되었으며, 높은 shear-thinning 거동을 가지고 있는 것으로 나타났다. 특정한 온도 범위($25{\sim}70^{\circ}C$)에서 전분 페이스트의 겉보기 점도($\eta_{a,100}$)의 온도 의존성은 Arrhenius 모델식에 잘 적용되었으며 초산 고구마전분이 천연전분에 비해 더 낮은 온도 의존성을 나타내었다. 전분 페이스트 이수현상 측정에서는 초산화에 의해 이수현상이 감소하는 것으로 나타났으며, 치환도가 증가할수록 낮아지는 것으로 나타났다. 겔 강도 측정에서도 초산화에 의해 겔 강도가 감소하는 것으로 나타났으며, 치환도가 증가할수록 겔 강도는 감소하는 것으로 나타났다. 페이스트 투과도(% T) 측정에서 초산전분은 천연전분 보다 투과도가 높은 것으로 나타났다.