• International Journal of Precision Engineering and Manufacturing
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• 제9권2호
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• pp.84-92
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• 2008

Free-form shapes which were once considered as an aesthetic feature are now an important functional requirement. CNC industries are looking for a compact solution for reproducing free-form shapes as conventional interpolation models are inadequate, The parametric curve interpolator developed in the last decade has clearly emerged as favorite among its contemporaries in recent years, At present intense research has been done on parametric curve interpolators and interesting developments are reported. Out of the various parametric representations for curves and surfaces, NURBS has been standardized and widely used in free-form shape design. This paper presents a review of various methods of parametric interpolation for NURBS and discusses the salient features, problems and solutions. Recent approaches on variable feedrate interpolation, parameter compensation are also reviewed and research trends are addressed finally.

• Archives of Reconstructive Microsurgery
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• 제8권2호
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• pp.163-169
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• 1999

Purpose : To evaluate the efficacy of free musculocutaneous flap in the treatment of chronic osteomyelitis with drain sinus. Materials and Methods : Eighteen patients (sixteen male and two female) whose average age was 42.6 years were followed for an average of 3.5 years after free musculocutaneous flap for chronic osteomyelitis with drain sinus tract. The period of time between the initial injury and this procedure was average 10.6 years. The patients had an average of 4.1 prior surgical procedure before it. Results : Sixteen(88.9%) of the eighteen free musculocutaneous flap survived, including thirteen of latisimus dorsi flap, three of scapular free flap with muscle, two of medial plantar flap with abductor muscle. Sixteen cases had no evidence of recurrence at follow up. Conclusion : Free musculocutaneous flap is an extremely successful and reliable procedure for the treatment of chronic osteomyelitis with drain sinus. This flap with microvascular anastomses is highly vascularized through its own intrinsic blood vessels, which has the advantage of obliterating dead space and providing improved vascularity to the relatively ischemic recipient site and covering the skin defect.

• 한국환경과학회지
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• 제21권3호
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• pp.351-362
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• 2012

The purpose of this study is to make a Result analysis on the free inquiry Method of elementary school student. also, this study investigates how free inquiry activities effect the science Process skills and environment-friendly attitudes of elementary school student For this study 150, sixth year, elementary school students from Busan city were selected. The inquiry examined the effectiveness of each of the following free inquiry methods: the PBL inquiry, the Project inquiry, the IIM inquiry, the small group inquiry and the science notebooks inquiry. The students were divided into groups in which they incorporated the respective methods into their practice. Test showed the following results: The environmental class which applies a free inquiry method(PBL inquiry, Project inquiry, IIM inquiry, small group inquiry and science notebooks) was effective in science process skills improvement. Second, The environmental class which applies a free inquiry method((PBL inquiry, Project inquiry, IIM inquiry, small group inquiry and science notebooks) was effective in environment-friendly attitude improvement.

• Natural Product Sciences
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• 제16권3호
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• pp.169-174
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• 2010

The seed of Phaseolus calcaratus Roxburgh (PHCR) is traditionally used for anti-pyretic and antiinflammatory effects. Although these effects are believed to be related to its antioxidant potential, little information is available for the mechanisms by which PHCR seed might scavenge free radicals or otherwise act as an antioxidant. In the present study, we purified some fractions from the ethanol extract of PHCR seed and evaluated each fraction's ability to scavenge free radicals generated by cell-free systems. We also identified active compound that is putatively responsible for free radical scavenging by analyzing NMR spectra. PHCR samples exhibited a concentration-dependent radical scavenging activity against hydroxyl radicals, superoxide anions, and DPPH radicals. Of the samples tested, a methanol-eluted sub-fraction from the PHCR extract, named $FF_4$, scavenged these radicals more effectively than the other fractions. We identified catechin-7-O-$\beta$-Dglucopyranoside as the active compound responsible for free radical scavenging potential of $FF_4$.

• Molecules and Cells
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• 제37권6호
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• pp.473-479
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• 2014

Spermatogonial stem cells (SSCs, also called germline stem cells) are self-renewing unipotent stem cells that produce differentiating germ cells in the testis. SSCs can be isolated from the testis and cultured in vitro for long-term periods in the presence of feeder cells (often mouse embryonic fibroblasts). However, the maintenance of SSC feeder culture systems is tedious because preparation of feeder cells is needed at each subculture. In this study, we developed a Matrigel-based feeder-free culture system for long-term propagation of SSCs. Although several in vitro SSC culture systems without feeder cells have been previously described, our Matrigel-based feeder-free culture system is time- and cost-effective, and preserves self-renewability of SSCs. In addition, the growth rate of SSCs cultured using our newly developed system is equivalent to that in feeder cultures. We confirmed that the feeder-free cultured SSCs expressed germ cell markers both at the mRNA and protein levels. Furthermore, the functionality of feeder-free cultured SSCs was confirmed by their transplantation into germ cell-depleted mice. These results suggest that our newly developed feeder-free culture system provides a simple approach to maintaining SSCs in vitro and studying the basic biology of SSCs, including determination of their fate.

• Bulletin of the Korean Chemical Society
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• 제24권9호
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• pp.1351-1353
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• 2003

• Architectural research
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• 제16권4호
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• pp.157-166
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• 2014

This research investigates the design process of free-form architecture to understand the design strategy and changing factors during the development phase and the cause for them. It is aimed to foresee the changing factors from the design process and to reduce design changes. It analyzes the design changes of free-form architecture based on projects with finalized documentation or under construction in South Korea. Many free-form shapes of the free-form architectures have to be adjusted to rigid-form in order to satisfy function and be economical to build. The research finds three patterns in design changes. First, from the factors for design changes: function, constructability, design, program add/subtract, efficiency, circulation; Function and Constructability are the higher factors compared with the rest. The two are the design changes suitable for actual usage and cost savings. Second, each project has different predominant factors for design changes as the degree of free-form is different. Contrary to initial expectation, the greater the degrees of free-form of the competition scheme, the higher the rate of Function among the factors for design changes. Constructability is higher when the degree of the free-form is less than others. It means that the lower the degree of the free-form, the more properly planned the space of the building is. Last, Constructability of free-form architecture is considered during the earlier design phase than definite-form, one by which the design changes by comparing 'Before fixed Space Program' (BSP) and 'After fixed Space Program' (ASP) design changes. The research would be helpful as a reference for setting up competition guidelines to reduce trial and error during the design process.

• 한국막학회:학술대회논문집
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• 한국막학회 2004년도 추계 총회 및 학술발표회
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• pp.125-128
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• 2004

• Journal of Mechanical Science and Technology
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• 제18권8호
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• pp.1435-1450
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• 2004

Experimental data are presented which describe the effects of a combustor-level high free-stream turbulence on the near-wall flow structure and heat/mass transfer on the endwall of a linear high-turning turbine rotor cascade. The end wall flow structure is visualized by employing the partial- and total-coverage oil-film technique, and heat/mass transfer rate is measured by the naphthalene sublimation method. A turbulence generator is designed to provide a highly-turbulent flow which has free-stream turbulence intensity and integral length scale of 14.7% and 80mm, respectively, at the cascade entrance. The surface flow visualizations show that the high free-stream turbulence has little effect on the attachment line, but alters the separation line noticeably. Under high free-stream turbulence, the incoming near-wall flow upstream of the adjacent separation lines collides more obliquely with the suction surface. A weaker lift-up force arising from this more oblique collision results in the narrower suction-side corner vortex area in the high turbulence case. The high free-stream turbulence enhances the heat/mass transfer in the central area of the turbine passage, but only a slight augmentation is found in the end wall regions adjacent to the leading and trailing edges. Therefore, the high free-stream turbulence makes the end wall heat load more uniform. It is also observed that the heat/mass transfers along the locus of the pressure-side leg of the leading-edge horseshoe vortex and along the suction-side corner are influenced most strongly by the high free-stream turbulence. In this study, the end wall surface is classified into seven different regions based on the local heat/mass transfer distribution, and the effects of the high free-stream turbulence on the local heat/mass transfer in each region are discussed in detail.

• The Korean Journal of Physiology and Pharmacology
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• 제2권2호
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• pp.193-199
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• 1998

To elucidate the mechanism involved in the cerebral vascular spasm following subarachnoid hemorrhage (SAH), the effects of the cerebrospinal fluid (CSF) obtained from the SAH patients on the resting tension and its influence on the contractile responses to various vasoactive agents and to hypoxia were investigated in isolated porcine cerebral arteries. All the CSFs containing hemoglobin (Hb) produced contraction and some Hb-free CSFs also elicited contraction. When the Hb-free CSF was separated by microfilter, the filtrate of ＜30,000 MW did not produce contraction, while the fraction above 30,000 MW elicited more marked contractile responses than the unfractionated CSF. The CSF contraction was significantly attenuated in the presence of indomethacin or nimodipine, whereas the contractions induced by KCl, prostaglandin $F_{2{\alpha}}$ ($PGF_{2{\alpha}}$), or endothelin-1 (ET-1) were not affected by the CSF pretreatment. However, the contractile responses induced by 5-hydroxytryptamine (5-HT) and phenylephrine (PE) were markedly potentiated by the pretreatment. Hypoxia-induced vasoconstriction was significantly potentiated by the pretreatment with either unfractionated CSF or the CSF fraction of above 30,000 MW. These results suggest that unknown vasocontractile substance(s) exists in the Hb-free CSF and that the substance, with its MW above 30,000, is activated by hypoxia and acts synergistically with 5-HT and PE, and that extracellular calcium influx and cyclooxygenase are also involved in the cerebral vasoconstrictory effect of Hb-free CSF.