Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Free Radical Scavenging Activity of the Seed of Phaseolus calcaratus Roxburgh

  • Fang, Minghao (Department of Orthodontics and Institute of Oral Biosciences, BK21 program and School of Dentistry, Chonbuk National University) ;
  • Cho, Hyoung-Kwon (Center for Health Care Technology development, HanPoong Pharmaceutical Co. Ltd.) ;
  • Ahn, Yun-Pyo (Department of Orthodontics and Institute of Oral Biosciences, BK21 program and School of Dentistry, Chonbuk National University) ;
  • Ro, Sang-Jeong (Department of Orthodontics and Institute of Oral Biosciences, BK21 program and School of Dentistry, Chonbuk National University) ;
  • Jeon, Young-Mi (Department of Orthodontics and Institute of Oral Biosciences, BK21 program and School of Dentistry, Chonbuk National University) ;
  • Whang, Wan Kyuun (College of Pharmacy, Chung-Ang University) ;
  • Lee, Jeong-Chae (Department of Orthodontics and Institute of Oral Biosciences, BK21 program and School of Dentistry, Chonbuk National University)
  • Received : 2010.07.02
  • Accepted : 2010.08.26
  • Published : 2010.09.30
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Abstract

The seed of Phaseolus calcaratus Roxburgh (PHCR) is traditionally used for anti-pyretic and antiinflammatory effects. Although these effects are believed to be related to its antioxidant potential, little information is available for the mechanisms by which PHCR seed might scavenge free radicals or otherwise act as an antioxidant. In the present study, we purified some fractions from the ethanol extract of PHCR seed and evaluated each fraction's ability to scavenge free radicals generated by cell-free systems. We also identified active compound that is putatively responsible for free radical scavenging by analyzing NMR spectra. PHCR samples exhibited a concentration-dependent radical scavenging activity against hydroxyl radicals, superoxide anions, and DPPH radicals. Of the samples tested, a methanol-eluted sub-fraction from the PHCR extract, named $FF_4$, scavenged these radicals more effectively than the other fractions. We identified catechin-7-O-$\beta$-Dglucopyranoside as the active compound responsible for free radical scavenging potential of $FF_4$.

Keywords

References

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