• Title/Summary/Keyword: free proline

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Analysis of the Free Amino Acids and Volatile-Flavor Compounds in the Commercial Brown-Rice Vinegar Prepared via Static Acetic-Acid Fermentation (시판 정치배양 현미식초의 유리아미노산 및 휘발성 향기성분 특성 분석)

  • Kim, Gui-Ran;Yoon, Sung-Ran;Lee, Su-Won;Jeong, Mi-Seon;Kwak, Ji-Young;Jeong, Yong-Jin;Yeo, Soo-Hwan;Kwon, Joong-Ho
    • Food Science and Preservation
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    • v.18 no.5
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    • pp.803-810
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    • 2011
  • This study was performed to evaluate the quality of the commercial brown-rice vinegars of South Korean and Japanese origins, which were prepared via static acetic-acid fermentation. The major free amino acids of Korean brown-rice vinegars were proline, glutamic acid and phenylalanine, while those of the Japanese were proline, valine, phenylalanine, lysine, ${\gamma}$-aminobutyric acid, alanine and isoleucine. The ${\gamma}$-aminobutyric acid (GABA) and total amino acids (3686.37~4212.27 mg%) contents were found to be significantly higher in the Japanese than in the Korean brown-rice vinegars. The key volatile compounds of the Korean brown-rice vinegars, analyzed with GC-MS, were acetic acid, benzaldehyde, phenethyl alcohol and phenethyl acetate while those of the Japanese brown-rice vinegars were acetic acid, ethyl acetate, ethyl alcohol, isoamyl acetate, phenethyl acetate and benzaldehyde. The volatile patterns of the Korean and Japanese commercial brown-rice vinegars were effectively distinguished from each other using an electronic nose, through which it was also elucidated that the volatiles profiles were similar among the Japanese vinegars but were different among the Korean vinegars.

AN ELECTRON MICROSCOPIC RADIOAUTOGRAPHIC STUDY OF PROTEIN SYNTHESIS IN VITRO IN THE PALATAL MUCOSA OF THE RAT

  • KIM Hyun Joo
    • Journal of Korean Academy of Oral and Maxillofacial Radiology
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    • v.17 no.1
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    • pp.51-87
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    • 1987
  • The incorporation of ³H-proline by epithelial and connective tissue elements of rat palatal mucosae was studied in order to investigate the relative levels of protein synthesis by the epithelium and underlying connective tissue cells. Following a sixty minutes incorporation of the radioactive tracer in vitro, it was found that the suprabasal cells had most grains per unit area. Furthermore, the grains were more concentrated over the cytoplasm than the nucleus. This was in contrast with the labeling of basal cells which had twice as many grains over the nucleoplasm than that over the cytoplasm. In intermediate cells; i.e., the spinous layer, the number of silver grains per unit area was decreased from that of the suprabasal cells. In areas where desmosomes were more prominent, many grains were in touch with such desmosomes. However, the labeling appeared to be reduced as soon as the cells became flattened. Moreover, the epidermal keratohyalin granules were relatively free of grains. Except for certain intercellular surfaces the keratinized cells were generally free of grains. On the connective tissue side, silver grains were primarily localized over the fibroblasts with occasional grains being found over palatal muscle cells, neural elements and so on. Most grains over collagenous fibers were found in relation to mature collagen fibrils. Thus, protein synthesis in isolated mucosae of the rat palate appeared to take place both in epithelial and connective elements. There were no apparent tissue alterations caused by the in vitro incorporation procedure utilized under conditions of this study.

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A Study on Nitrogen Compounds and the Vicissitude of Free Amino Acids in Mong-bean Saute (녹두(綠豆)지짐에 있어서의 함질소화합물(含窒素化合物) 특(特)히 유리(遊離) 아미노산(酸)의 소장(消長)에 관(關)하여)

  • Kang, Young-Hee;Lee, Jung-Ock;Chung, Ha-Boon;Lee, Ki-Yull
    • Journal of Nutrition and Health
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    • v.4 no.1
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    • pp.63-67
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    • 1971
  • The nitrogen content and free amino acids were determined from the saute of mong bean paste which is one of the important protein sources on Korean diet. During the saute' process of mong-bean paste, valine, r-aminobutyrate, glutamine, arginine, methionine, and unknown acids were lost, but, proline, lysine, homoserine, and tyrosine were detected.

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Identification of Growth Stimulatory Compound in the Mixed Culture of Lactobacillus helveticus YM-1 and Streptococcus thermophilus CH-1 in Milk (Lactobacillus helveticus YM-1 과 Streptococcus thermophilus CH-1의 혼합배양액 중에 함유된 생육촉진물질의 확인)

  • Yoon, Sung-Sik;Yu, Ju-Hyun
    • Korean Journal of Food Science and Technology
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    • v.18 no.6
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    • pp.492-496
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    • 1986
  • A compound stimulatory to the growth of S. thermophilus CH-1 was isolated from the cell-free filtrate of L. helveticus YM-1 in milk medium. The stimulant was identified as a peptide with a molecular weight of approximately 5000 and exhibited positive ninhydrin reaction. Some kinds of amino acids confirmed as aspartic acid, alanine, valine, glutamic acid, lysine, proline, leucine were rich in the stimulatory peptide hydrolysate. Among them, glutamic acid was most abundant. Judging from bioautographic results, glutamic acid and phenylalanine were expected to exert an important role for the stimulation.

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Analysis of Minerals, Amino Acids,and Vitamin of Lespedeza cuneata (비수리의 미네랄, 이미노산, 비타민 분석)

  • Ding, Ji-Lu;Lim, Ik-Jae;Lee, Hee-Duck;Cha, Wol-Suk
    • KSBB Journal
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    • v.21 no.6 s.101
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    • pp.414-417
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    • 2006
  • For developing functional biomaterials, chemical analysis of Lespedeza cuneata including minerals, amino acids and vitamin were investigated. Minerals of L. cuneata were found to be calcium, potassium, magnesium, phosphorus, sodium, iron, manganese, zinc and copper. Among the free amino acid, proline was 33.77 mg% that were 67.2% of free amino acids and essential amino acids were 7.49 mg%. Total amino acids were analyzed as 2,817 mg% and the content of glutamic acid (496.00 mg%) was highest. In case of vitamin, the highest components was vitamin E with 33.03 mg%.

Effective Components on the Taste of Ordinary Korean Soy Sauce (한국재래식 간장의 맛에 영향을 미치는 성분)

  • 김종규;정영건;양성호
    • Microbiology and Biotechnology Letters
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    • v.13 no.3
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    • pp.285-287
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    • 1985
  • To investigate effective constituents of the many taste components in ordinary Korean soy sauce, we analyzed free amino acids, organic acids, free sugars and saline as taste components in ordinary Korean soy sauce, and determined sensory score of the ordinary Korean soy sauce taste with 45 persons of the trained pannels. The relationships between original data transformed with variables and sensory score of the ordinary Korean soy sauce were analyzed by stepwise multiple regression analysis. Eighty five percents of the ordinary Korean soy sauce taste is affected by twenty one kinds (Isoleucine, Leucine, Valine, NaCl, Lactic acid, Alanine, Phenylalanine, Tartaric acid, Sugar(\ulcorner), Proline, Malic acid, Glycine, Tryptophan, Arginine, Glutaric acid, Maltose, Histidine, Glucose, Fructose and Serine) of the taste components by stepwise multiple regression analysis of original data. Eighty one percents of the ordinary Korean soy sance taste is affected by sixteen kinds (Lactic acid, NaCl, Fumaric.Succinic acid, Tyrosine, Tartaric acid, Glycine, Malonic acid, Malic acid, Tryptophan, Glutaric acid, Methionine, Histidine, Cysteine, Maltose, Fructose and (Glutamic acid) of the taste components by stepwise multiple frgression analysis of original data transformed with square root. Eighty five percents of the ordinary Korean soy sauce taste is affected by nineteen kinds (Fumaric.Succinic acid, Lactic acid, Phenylalanine, NaCl, Tyrosine, Sugar(\ulcorner), Tartaric acid, Leucine, Glutaric acid, Methionine, Glycine, Tryptophan, Histidine, Proline, Cysteine, Glutamic acid, Maltose, Threonine and Oxalic acid) of the taste components by stepwise multiple regression analysis of original data transformed with logarithm.

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FREE AMINO ACID CONTENT IN THE EXTRACT OF CORAL DAMSELFISH, CHROMIS NOTATUS (자리돔 엑스분의 유리아미노산)

  • HA Jin-Hwan;LEE Eung-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.12 no.4
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    • pp.241-243
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    • 1979
  • Coral damselfish (demoiselle), Chromis notatus, has been one of the most tasty sea foods in Jeju-Do, Korea, The free amino acids in tile extracts of Chromis notatus were analysed by amino acid autoanalyser. In the extracts, taurine, lysine, glycine and alanine were abundant, holding $30.3\%,\;22.7\%,\;16.0\%\;,\;11.5\%$ of total amino acid content, respectively and followed threonine, glutamic acid and serine, in order. While methionine, histidine, leucine, isoleucine, valine were poor and aspartic acid and proline were trace in content.

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Studies on the Amino Acid Components of Korean Hazel Nut (한국산(韓國産) 개암 종실(種實)의 아미노산 조성(組成)에 관한 연구)

  • Kim, Mi-Ran;Ko, Young-Su
    • Korean Journal of Food Science and Technology
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    • v.13 no.1
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    • pp.1-5
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    • 1981
  • Free and total amino acids of Korean hazel nut (Corylus heterophylla Fisch. var. Japonica koidz.) were identified by amino acid autoanalyzer (Technicon PNC-1 type). The results indicate that among 16 kinds of all free amino acids contained in hazel nut, arginine was the richest, and then came glutamic acid, proline, alanine, tyrosine and valine in that order. From the total amino acids which were closely related with the nutritional valuation, arginine was in the richest amount and then came with glutamic acid, proline, leucine, aspartic acid and valine.

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Chemical Compositions of Dried Wild and Cultivated Codonopsis lanceolata (건조된 야생더덕과 경작더덕의 화학성분)

  • Lee, Suk-Kun
    • Applied Biological Chemistry
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    • v.27 no.4
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    • pp.225-230
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    • 1984
  • Chemical composition of dried roots of wild and cultivated Codonopsis lanceolata has been investigated. General composition was similar in both Codonopsis lanceolata. Free sugars from the root were fructose, glucose and sucrose. The contents of the sugars were higher in the cultivated than in the wild. Maltose was detected only in the cultivated and its level was 0.05%.. Free amino acids were consisted of 16 amino acids: lysine, histidine, arginine aspartic acid, threonine, serine, glutamic acid. proline, glycine, alanine, valine, methionine, isoleucine, leucine, tyrosine and phenylalanine. No significant difference in the contents was found between the wild root and the cultivated. Free fatty acids were palmitate, linoleate and linolenate, and the contents of those acids were higher in the cultivated root than in the wild. The contents of crude saponin were 1.5% in the wild root and 1.4% in the cultivated, respectively.

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Studies on the Components of Vegetables (야채류(野菜類)의 성분(成分)에 관(關)한 연구(硏究))

  • Cho, Soo-Yeul;Kim, Seuk-Hwan
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.5 no.1
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    • pp.61-64
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    • 1976
  • A survey of the free amino acids and organic acids in the shoot of Phyllostachys edulis was made by means of amino acid autoanalyzer and gas chromatograph. The results of the survey are summerized as follows. 1. Eighteen amino acids found in bamboo shoot were lysine, histidine, arginine, tryptophan, aspartic acid, glutamic acid, threonine, serine, proline, glycine, alanine, valine, leucine, isoleucine, methionine, tyrosine and phenylalanine, and an unknown was found. Serine showed the highest amount and more than about 44% of total free amino acids. 2. Oxalic acid was the major organic acid, and formic acid, acetic acid, maleic acid, succinic acid, fumaric acid, citric acid, tartaric acid and sorbic acid were determined, and two unknown were found.

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