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Effective Method for Extraction of Cell-Free DNA from Maternal Plasma for Non-Invasive First-Trimester Fetal Gender Determination: A Preliminary Study

  • Lim, Ji-Hyae;Park, So-Yeon;Kim, Shin-Young;Kim, Do-Jin;Kim, Mee-Jin;Yang, Jae-Hyug;Kim, Moon-Young;Kim, Min-Hyoung;Han, Ho-Won;Choi, Kyu-Hong;Ryu, Hyun-Mee
    • Journal of Genetic Medicine
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    • v.7 no.1
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    • pp.53-58
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    • 2010
  • Purpose: To find the most effective method for extraction of cell-free DNA (cf-DNA) from maternal plasma, we compared a blood DNA extraction system (blood kit) and a viral DNA extraction system (viral kit) for non-invasive first-trimester fetal gender determination. Materials and Methods: A prospective cohort study was conducted with maternal plasma collected from 44 women in the first-trimester of pregnancy. The cf-DNA was extracted from maternal plasma using a blood kit and a viral kit. Quantitative fluorescent-polymerase chain reaction (QF-PCR) was used to detect the SRY gene and AMEL gene. The diagnostic accuracy of the QF-PCR results was determined based on comparison with the final delivery records. Results: A total of 44 women were tested, but the final delivery record was only obtained in 36 cases which included 16 male-bearing and 20 female-bearing pregnancies. For the blood kit and viral kit, the diagnostic accuracies for fetal gender determination were 63.9% (23/36) and 97.2% (35/36), respectively. Conclusion: In non-invasive first-trimester fetal gender determination by QF-PCR, using a viral kit for extraction of cf-DNA may result in a higher diagnostic accuracy.

Structural Factors Influencing the Quality Management Activities in Nursing Homes (노인요양시설의 질 관리 활동에 영향을 주는 구조적 요인 분석)

  • Lee, Tae-Wha;Chung, Jane
    • Journal of Korean Academy of Nursing Administration
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    • v.16 no.2
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    • pp.162-171
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    • 2010
  • Purpose: Nursing home quality indicators have been focused widely on result outcomes, not for the environment in that quality of service are delivered, This study aimed to examine structural factors influencing quality management activities in nursing homes. Method: Sample was 170 nursing homes responded to the survey questionnaire which was distributed to the 543 nursing homes nation-wide, Data were collected on structural characteristics, types of services, and quality management activities, Data were analyzed with the descriptive statistics, Pearson correlations, and multiple regression. Result: Most of the nursing homes were operated as free of charge by the social welfare ownership. Average number of residents was 52.1 with severe and mild dementia and bedridden status, In terms of quality management activities, 34% of the sample had CQI committee that focused their activities on services delivery process, performance appraisal, record keeping regularly. 30.6% of quality management activities were accounted for by the number of residents with dementia, the ratio of RN to residents, rehabilitation services, and social wefare services in nursing homes. Conclusion: We recommend that more comprehensive quality management activities should be developed as process quality indicators in conjunction with the outcome indicators.

Signaling for Free-view Broadcast Service based on ROUTE/DASH in ATSC 3.0 (ATSC 3.0에서 ROUTE/DASH 기반의 자유시점 방송 서비스를 위한 시그널링)

  • You, Dongho;Jang, Jungyup;Kim, Namtae;Seo, Bong-seok;Jung, Eun-young;Kim, Dong Ho
    • Proceedings of the Korean Society of Broadcast Engineers Conference
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    • 2016.06a
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    • pp.46-47
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    • 2016
  • 본 논문은 북미의 차세대 지상파 방송 표준인 ATSC 3.0에서 고려하고 있는 ROUTE(Real-time Object Delivery over Unidirectional Transport) 및 MPEG-DASH(Dynamic Adaptive Streaming over HTTP)를 기반으로 자유시점 방송 서비스를 송 수신하기 위한 SLS(Service Layer Signaling)을 제안한다. 특별히 ATSC 3.0과의 역호완성을 위하여 기존의 ROUTE/DASH의 SLS는 그대로 유지한 채 자유시점 방송을 위한 정보만 추가하여 기존의 SLS와 상호동작이 되도록 설계하였다. 따라서 본 논문에서 제안하는 내용은 향후 ATSC 3.0와 같이 IP(Internet Protocol)을 기반으로 SLS을 수행하는 표준규격과 역호환성을 유지한 채 자유시점 방송서비스를 할 수 있을 것으로 기대된다.

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Painless Microjet Injector Using Laser Pulse Energy (레이저 펄스 에너지를 이용한 무통증 마이크로젯 약물전달시스템)

  • Yoh, Jai-Ick;Han, Tae-Hee;Hah, Jung-Moo
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.35 no.5
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    • pp.547-550
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    • 2011
  • We have developed a laser-based needle-free liquid drug-injection device. A laser beam is focused inside the liquid contained in the rubber chamber of a micro-scale. The focused laser beam causes explosive bubble growth, and the sudden volume increase in a sealed chamber drives a microjet of liquid drug through the micronozzle. The exit diameter of a nozzle is less than 100 ${\mu}m$, and we verify that the injected microjet is fast enough to penetrate soft human tissue. In the experiment, the microjet penetrated a 5% gelatin-water solution that replicates the human thrombus and pork-fat tissue.

A Multi-path Dynamic Source Routing Protocol for Mobile Ad-Hoc Networks (이동 애드 혹 네트워크를 위한 다중경로 동적 소스 라우팅 프로토콜)

  • Lim Hwa-Jung;Tscha Yeong-hwan
    • The KIPS Transactions:PartC
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    • v.12C no.1 s.97
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    • pp.111-120
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    • 2005
  • A mobile ad-hoc network is an autonomous system of mobile nodes which are free to move around randomly and organize themselves arbitrarily, hence a routing protocol that handles the dynamic network topology changes is required for the network. In this paper we present a multi -Path on-demand routing protocol called R-DSR (Robust Dynamic Source Routing), an extension to the existing IETF protocol DSR. The proposed protocol has it that every pair of 2-hop away nodes on the single route is additionally connected via an alternative node. Throughout mathematical analysis we show the proposed protocol reveals higher message delivery rate than that of the Das's multi-path protocol, currently known as one of the most typical approaches related to DSR.

A Wire-overhead-free Reset Propagation Scheme for Millimeter-scale Sensor Systems

  • Lee, Inhee;Bang, Suyoung;Kim, Yejoong;Kim, Gyouho;Sylvester, Dennis;Blaauw, David;Lee, Yoonmyung
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.17 no.4
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    • pp.524-533
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    • 2017
  • This paper presents a novel reset scheme for mm-scale sensing systems with stringent volume and area constraints. In such systems, multi-layer structure is required to maximize the silicon area per volume and minimize the system size. The multi-layer structure requires wirebonding connections for power delivery and communication among layers, but the area overhead for wirebonding pads can be significant. The proposed reset scheme exploits already existing power wires and thus does not require additional wires for system-wide reset operation. To implement the proposed reset scheme, a power management unit is designed to impose reset condition, and a reset detector is designed to interpret the reset condition indicated by the power wires. The reset detector uses a coupling capacitor for the initial power-up and a feedback path to hold the developed supply voltage. The prototype reset detector is fabricated in a $180-{\mu}m$ CMOS process, and the measurement results with the prototype mm-scale system confirmed robust reset operation over a wide range of temperatures and voltages.

A Study on Homepage Analysis of Family Restaurant thorough Marketing Mix (마케팅 믹스를 활용한 패밀리레스토랑의 홈페이지 분석에 관한 연구)

  • 진양호;전진화
    • Culinary science and hospitality research
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    • v.9 no.2
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    • pp.44-63
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    • 2003
  • This study is about internet marketing of family restaurant through marketing mix and we found out the way which can make to activate by means of analysis of family restaurant's homepage. This study was done as following precedure. First, we've compared between characteristic was based on contents distinctive quality of family restaurant's homepage through the marketing mix. And in the next step, we've compared and ana lysed homepage of family restaurant which was chosed by the internet surfing. In the last, we evaluate strategy of marketing mix which is going well in the each field of business and then research of the way can improve internet-marketing. We draw the findings which is seperated by goods, price, promotion, distribution, other service according to marketing constituents. As a result of study, it is found that each company has given much more weight on some part in internet marketing's field of activity. In general, they put an emphasis on activity of goods and promotion. On the other hands they don't consider prices and delivery to be important compared with other parts. One of the most important things about internet-marketing is that fast and exact feedback and community makes each firm to be able to help to communicate with customer. It's like off-line-restaurant make use of free-coupon and an anniversary service.

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Computer Controller Design and Movement of Hovercraft Driving Apparatus (호버크래프트 추진장치의 컴퓨터 제어기 설계와 동작)

  • Baek, Dong-Hyun;Song, Ho-Bin;Cho, Moon-Tack
    • Fire Science and Engineering
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    • v.25 no.5
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    • pp.32-38
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    • 2011
  • Amphibious possible, and good fuel economy, so as leisure and transport has increased the use of hovercraft. Fire started in Korea, the prevalence is increasing in demand as the trend has been increasing steadily. In this paper, the hovercraft's forward and backward direction can be controlled in order to free the reverse bucket control system was developed. Control due to development by promoting the flow of air and turn right, turn left and easy to reverse the life-saving and stable at high speed, etc. has made possible the operation of hovercraft. The controller for the stability and fast response Fuzzy-PID method was used. To prove the validity of the proposed controller in Matlab simulation and the actual delivery at the firehouse, built into a model for the demonstration test was performed.

A Study on the Free formaldehyde in Nonwoven Fabrics -with emphasis on the fusible bonded webs- (부직포 중에 잔존하는 유리 Formaldehyde에 관한 연구 -접착심지를 중심으로-)

  • Song Myoung Kyoun
    • Journal of the Korean Society of Clothing and Textiles
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    • v.3 no.2
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    • pp.9-14
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    • 1979
  • As the fabric merchandises become more diversified and the pre-condensates of resin are used in increasing amount to enhance the quality of so-called 'easy care' in such fabric goods, form-aldehyde, which is extricated from nonwoven fabrics, has posed problems. The result of the study conformed, firstly that the fusible bonded webs sold in the current market contained a considerable amount of formaldehyde, i.e. the minimum of 552$\mu$g/g and the maximum of 2,123$\mu$g/g. Secondly, formaldehyde fond in many of such fabric goods was unable to be removed completely even after washed for, three times. Thirdly, the degree of permeation of HCHO into other part of the fabric proved to be high and. when kept in a poly bag over 10days. the amount of formaldehyde tended to increase. Such experiment, therefore, demonstrates that the methods of delivery and storage employed in the current distribution structure must be thoroughly re-examined. The conclusion of this study shows that we should be more concerned with the adverse effects of formaldehyde for the health of consumers. let alone the increase of export of such merchandises.

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Net Shape Forming Process for Ball Stud Using High Strength Micro-Alloyed Cold Forging Steel (냉간 비조질강을 이용한 볼 스터드의 정형가공 공정연구)

  • Yoon, D.J.;Choi, H.J.;Lee, H.W.;Lee, G.A.;Jang, B.L.;Seo, S.L.;Choi, S.
    • Transactions of Materials Processing
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    • v.15 no.8 s.89
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    • pp.562-567
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    • 2006
  • Micro-alloyed steel or heat-treatment-free used in clean technology have been replacing for conventional quenched-and-tempered structural steels since the micro-alloyed forging steel was developed in early 1970s in Germany for saving money of heat treatment, simplified process, short delivery and good productivity. In this paper, ball stud assembled in steering system for automobile was selected to compare conventional process making heat treatment with new process using high strength micro-alloyed steel without heat treatment. The conventional process for ball stud was composed of a total of 6 steps including upsetting, forward extrusion, machining, burnishing and tread rolling with heat treatment and shot blasting. As opposed to conventional process, newly proposed process for ball stud using the clean technology without heat treatment is simplified such as forward extrusion, heading, upsetting, forming having a flange shape and tread rolling. Also net shape forming process to achieve specified process not to include machined step fur manufacturing the ball stud was applied to newly simplified process since micro-alloyed steel is difficult to be formed.