• The KIPS Transactions:PartC
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• v.13C no.5 s.108
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• pp.567-574
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• 2006

VG-AnyLAN is a local area network standard developed by the IEEE 802.12 project. While preserving the frame format of IEEE 802.3, VG-AnyLAN adopts a new medium access control called Demand Priority where transmission requests of stations are arbitrated by a control hub in a round-robin manner. Unlike CSMA/CD which is the medium access control of IEEE 802.3, the Demand Priority, while providing the maximum bound on the packet delay, does not put the limit on the network segment size. In this paper, we analyze the delay and the channel utilization performances of the medium access control of IEEE 802.12 VG-AnyLAN. We develope an analytic model of the system under assumptions that each station generates traffic of the equal priority and that the packets are of fixed length. Using the analytic model, we obtain the recursive expression of the average channel utilization and the average access delay The numerical results obtained via analysis are compared to the simulation results of the system for a partial validation of our analysis.

• Journal of Microbiology and Biotechnology
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• v.15 no.4
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• pp.749-755
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• 2005

The ccpA gene encoding catabolite control protein A (CcpA) of Leuconostoc mesenteroides SYl, a strain isolated from kimchi, was cloned, sequenced, analyzed for transcript, and overexpressed in Escherichia coli. The ccpA ORF (open reading frame) is 1,011 bp in size, which can encode a protein of 336 amino acid residues with a molecular mass of 36,739 Da. The transcription start site was mapped at a position 49 nucleotides upstream of the start codon, and promoter sequences were also identified. The putative cre site overlapped with the -35 promoter sequence. The deduced amino acid sequence of the CcpA contained the helix-turn-helix motif found in many DNA-binding regulatory proteins. CcpA from 1. mesenteroides SY1 had $54.6\%$ identity with CcpA from Lactobacillus casei. The Northern blot experiment showed that ccpA was transcribed as a single 1.1 kb transcript, and transcription was repressed when grown on media containing glucose. CcpA was overproduced in E. coli BL21(DE3) cells using the pET expression vector, and purified to an apparent homogeneity. Gel Mobility Shift Assay with purified CcpA and a DNA fragment containing the ere sequence of the $\alpha$-galactosidase gene (aga) from L. mesenteroides SY1 revealed that CcpA bound specifically to the cre site of aga.

• Journal of Communications and Networks
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• v.16 no.2
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• pp.172-182
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• 2014

In this paper, we investigate the fundamental performance limits of the cooperative sensing using energy detection by considering the unlimited number of sensing nodes. Although a lot of cognitive radio research so far proposed various uses of energy detection because of its simplicity, the performance limits of energy detection have not been studied when a large number of sensing nodes exist. First, we show that when the sensing nodes see the independent and identically distributed channel conditions, then as the number of sensing nodes N goes to infinity, the OR rule of hard decision achieves zero of false alarm Pf for any given target probability of detection $\bar{P_d}$ irrespective of the non-zero received primary user signal to noise ratio ${\gamma}$. Second, we show that under the same condition, when the AND rule of hard decision is used, there exists a lower bound of $P_f$. Interestingly, however, for given $\bar{P_d}$, $P_f$ goes to 1 as N goes to infinity. Third, we show that when the soft decision is used, there exists a way of achieving 100% utilization of secondary user, i.e., the sensing time overhead ratio goes to zero so does $P_f$.We verify our analyses by performing extensive simulations of the proposed unlimited cooperative sensing. Finally, we suggest a way of incorporating the unlimited cooperative sensing into a practical cellular system such as long term evolutionadvanced by exploiting the existing frame structure of absolute blank subframe to implement the in-band sensing.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.29 no.4A
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• pp.429-438
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• 2004

In this paper, a decision-feedback coherent code tracking loop is designed for WCDMA systems and its performance is analyzed in terms of jitter variance considering the effect of phase and symbol estimation errors for both AWGN and fading environments. An analytical closed-form formula for jitter variance is Int derived for AWGN environments as a function of a pulse-shaping filter, timing offset, signal-to-interference ratio, and loop bandwidth while involving the phase estimation error and bit error rate, and the upper bound of jitter variance is derived for fading environments. Finally a second-order coherent code tracking loop is designed with the DPCH frame format #13 of the WCDHA forward link selected as a target system, and its performance is evaluated by the closed-form formula and compared with the simulation results for both AWGN and Rayleigh fading environments.

• Journal of Microbiology and Biotechnology
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• v.29 no.4
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• pp.577-586
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• 2019

The engineered Aspergillus oryzae has a high NADPH demand for xylose utilization and overproduction of target metabolites. Glucose-6-phosphate dehydrogenase (G6PDH, E.C. 1.1.1.49) is one of two key enzymes in the oxidative part of the pentose phosphate pathway, and is also the main enzyme involved in NADPH regeneration. The open reading frame and cDNA of the putative A. oryzae G6PDH (AoG6PDH) were obtained, followed by heterogeneous expression in Escherichia coli and purification as a his6-tagged protein. The purified protein was characterized to be in possession of G6PDH activity with a molecular mass of 118.0 kDa. The enzyme displayed maximal activity at pH 7.5 and the optimal temperature was $50^{\circ}C$. This enzyme also had a half-life of 33.3 min at $40^{\circ}C$. Kinetics assay showed that AoG6PDH was strictly dependent on $NADP^+$ ($K_m=6.3{\mu}M$, $k_{cat}=1000.0s^{-1}$, $k_{cat}/K_m=158.7s^{-1}{\cdot}{\mu}M^{-1}$) as cofactor. The $K_m$ and $k_{cat}/K_m$ values of glucose-6-phosphate were $109.7s^{-1}{\cdot}{\mu}M^{-1}$ and $9.1s^{-1}{\cdot}{\mu}M^{-1}$ respectively. Initial velocity and product inhibition analyses indicated the catalytic reaction followed a two-substrate, steady-state, ordered BiBi mechanism, where $NADP^+$ was the first substrate bound to the enzyme and NADPH was the second product released from the catalytic complex. The established kinetic model could be applied in further regulation of the pentose phosphate pathway and NADPH regeneration of A. oryzae to improve its xylose utilization and yields of valued metabolites.

• Journal of The Korean Astronomical Society
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• v.52 no.3
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• pp.57-69
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• 2019

We present an analysis of the chemical abundances and kinematics of six low-mass dwarf stars, previously claimed to be candidate hypervelocity stars (HVSs). We obtained moderate-resolution (R ~ 6000) spectra of these stars to estimate the abundances of several chemical elements (Mg, Si, Ca, Ti, Cr, Fe, and Ni), and derived their space velocities and orbital parameters using proper motions from the Gaia Data Release 2. All six stars are shown to be bound to the Milky Way, and in fact are not even considered high-velocity stars with respect to the Galactic rest frame. Nevertheless, we attempt to characterize their parent Galactic stellar components by simultaneously comparing their element abundance patterns and orbital parameters with those expected from various Galactic stellar components. We find that two of our program stars are typical disk stars. For four stars, even though their kinematic probabilistic membership assignment suggests membership in the Galactic disk, based on their distinct orbital properties and chemical characteristics, we cannot rule out exotic origins as follows. Two stars may be runaway stars from the Galactic disk. One star has possibly been accreted from a disrupted dwarf galaxy or dynamically heated from a birthplace in the Galactic bulge. The last object may be either a runaway disk star or has been dynamically heated. Spectroscopic follow-up observations with higher resolution for these curious objects will provide a better understanding of their origin.

• IMMUNE NETWORK
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• v.24 no.1
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• pp.1.1-1.6
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• 2024

IL-18 binding protein (IL-18BP) was originally discovered in 1999 while attempting to identify an IL-18 receptor ligand binding chain (also known as IL-18Rα) by subjecting concentrated human urine to an IL-18 ligand affinity column. The IL-18 ligand chromatography purified molecule was analyzed by protein microsequencing. The result revealed a novel 40 amino acid polypeptide. To isolate the complete open reading frame (ORF), various human and mouse cDNA libraries were screened using cDNA probe derived from the novel IL-18 affinity column bound molecule. The identified entire ORF gene was thought to be an IL-18Rα gene. However, IL-18BP has been proven to be a unique soluble antagonist that shares homology with a variety of viral proteins that are distinct from the IL-18Rα and IL-18Rβ chains. The IL-18BP cDNA was used to generate recombinant IL-18BP (rIL-18BP), which was indispensable for characterizing the role of IL-18BP in vitro and in vivo. Mammalian cell lines were used to produce rIL-18BP due to its glycosylation-dependent activity of IL-18BP (approximately 20 kDa). Various forms of rIL-18BP, intact, C-terminal his-tag, and Fc fusion proteins were produced for in vitro and in vivo experiments. Data showed potent neutralization of IL-18 activity, which seems promising for clinical application in immune diseases involving IL-18. However, it was a long journey from discovery to clinical use although there have been various clinical trials since IL-18BP was discovered in 1999. This review primarily covers the discovery of IL-18BP along with how basic research influences the clinical development of IL-18BP.

• Journal of the Korean Society for Nondestructive Testing
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• v.27 no.5
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• pp.426-431
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• 2007

When a high-energy ultrasound propagates through a solid body that contains a crack or a delamination, the two faces of the defect do not ordinarily vibrate in unison, and dissipative phenomena such as friction, rubbing and clapping between the faces will convert some of the vibrational energy to heat. By combining this heating effect with infrared imaging, one can detect a subsurface defect in material in real time. In this paper a realtime detection of the brazing defect of thin Inconel plates using the UIR (ultrasonic infrared imaging) technology is described. A low frequency (23 kHz) ultrasonic transducer was used to infuse the welded Inconel plates with a short pulse of sound for 280 ms. The ultrasonic source has a maximum power of 2 kW. The surface temperature of the area under inspection is imaged by an infrared camera that is coupled to a fast frame grabber in a computer. The hot spots, which are a small area around the bound between the two faces of the Inconel plates near the defective brazing point and heated up highly, are observed. And the weak thermal signal is observed at the defect position of brazed plate also. Using the image processing technology such as background subtraction average and image enhancement using histogram equalization, the position of defective brazing regions in the thin Inconel plates can be located certainly.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.37 no.1
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• pp.1-15
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• 2000

To reconstruct 3- D shape, It is a common strategy to acquire multiple range Images from different viewpoints and integrate them into a common coordinates In this paper, we particularly focus on the registration and integration processes for combining all range Images into one surface model. For the registration, we propose the 2-step registration algorithm, which consists of 2 steps the rough registration step using all data points and the fine registration step using the high-curved data points For the integration, we propose a new algorithm, referred to as ‘multi-registration’ technique, to alleviate the error accumulation problem, which occurs during applying the pair-wise registration to each range image sequentially, in order to transform them into a common reference frame Intensive experiments are performed on the various real range data In experiments, all range images were registered within 1 minutes on Pentium 150MHz PC The results show that the proposed algorithms registrate and integrate multiple range Images within a tolerable error bound in a reasonable computation time, and the total error between all range Images are equalized with our proposed algorithms.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.34 no.6B
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• pp.595-604
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• 2009

The HCCA reserves the channel resources based on the mean data rate in IEEE 802.11e. It may cause either the waste of channel resource or the increase of transmission delay at MAC layer if the frame size is rapidly varied when a compressed mode video codec such as MPEG video is used. To solve these problems, it is developed that the packet scheduler allocates the wireless resource adaptation by according to the packet size. However, it is difficult to perform the admission control because of the difficulty with calculating the available resources. In this paper, we propose a CAC mechanism to solve the problem that may not satisfy the QoS by increasing traffic load in case of using EDCA. Especially, the proposed CAC mechanism calculates the EB of TSs using the traffic information transmitted by the application layer and the number of average transmission according to the wireless channel environment, and then determines the admission of the TS based on the EB. According to the simulation results of the proposed CAC mechanism, it admitted the TSs under the loads which are satisfied within the delay bound. Therefore, the proposed mechanism guarantees QoS of streaming services effectively.