• The Plant Pathology Journal
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• v.32 no.5
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• pp.414-422
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• 2016

Eucalyptus dieback disease, caused by Cylindrocladium scoparium, has occurred in last few years in large Eucalyptus planting areas in China and other countries. Rapid, simple, and reliable diagnostic techniques are desired for the early detection of Eucalyptus dieback of C. scoparium prior to formulation of efficient control plan. For this purpose, three PCR-based methods of nested PCR, multiplex PCR, loop-mediated isothermal amplification (LAMP) were developed for detection of C. scoparium based on factor 1-alpha (tef1) and beta-tubulin gene in this study. All of the three methods showed highly specific to C. scoparium. The sensitivities of the nested PCR and LAMP were much higher than the multiplex PCR. The sensitivity of multiplex PCR was also higher than regular PCR. C. scoparium could be detected within 60 min from infected Eucalyptus plants by LAMP, while at least 2 h was needed by the rest two methods. Using different Eucalyptus tissues as samples for C. scoparium detection, all of the three PCR-based methods showed much better detection results than regular PCR. Base on the results from this study, we concluded that any of the three PCR-based methods could be used as diagnostic technology for the development of efficient strategies of Eucalyptus dieback disease control. Particularly, LAMP was the most practical method in field application because of its one-step and rapid reaction, simple operation, single-tube utilization, and simple visualization of amplification products.

• The Plant Pathology Journal
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• v.35 no.3
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• pp.234-242
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• 2019

Thirty-four strains of bacteria were isolated from Phellodendron amurense. Using Nectria haematococca as an indicator strain, the best strain, B18, was obtained by the growth rate method. The morphological, physiological and biochemical characteristics of strain B18 and its 16S DNA gene sequence were identified, and the biocontrol effect of strain B18 was assessed in pot and field tests, as well as in a field-control test. Drilling methods were used to determine the antibacterial activity of metabolites from strain B18 and their effects on the growth of pathogen mycelia and spores. The best bacteriostatic rate was 85.4%. B18 can hydrolyse starch and oxidize glucose but does not produce gas; a positive result was obtained in a gelatine liquefaction test. According to 16S DNA gene sequencing, strain B18 is Bacillus methylotrophicus (GenBank accession number: MG457759). The results of pot and field-control trials showed 98% disease control when inoculating $10^8cfu/ml$ of the strain. The disease control effect of the B18 culture liquid (concentrations of $10^8$, $2{\times}10^6$, $10^6$, $5{\times}10^5$ and $2.5{\times}10^5cfu/ml$) in the field-control test was higher than 80%, and the cure rate of the original delivery solution was 96%. Therefore, in the practical forestry production, a $2.5{\times}10^5cfu/ml$ culture liquidshould be applied in advance to achieve good control effects.

• The Plant Pathology Journal
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• v.40 no.1
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• pp.1-15
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• 2024

The aim of this study was to isolate biocontrol bacteria that could antagonize brown rot of Dendrocalamus latiflorus, optimize the culture conditions, and develop an effective biocontrol preparation for brown rot of D. latiflorus. This study isolated a bacterium with an antagonistic effect on bamboo brown rot from healthy D. latiflorus rhizosphere soil. Morphology, molecular biology, and physiological biochemistry methods identified it as Bacillus siamensis. The following culturing media and conditions improved the inhibition effect of B. siamensis: the best culturing media were 2% sucrose, 1.5% yeast extract, and 0.7% potassium chloride; the optimal culturing time, temperature, pH, and inoculation amount were 48 h, 30℃, 6, and 20%. The optimum formula of the applying bacterial suspension was 14% sodium dodecyl benzene sulfonate emulsifier, 4% Na₂HPO₄·2H₂O, 0.3% hydroxypropyl methylcellulose thickener, and 20% B. siamensis. The pot experiment results showed the control effect of applying bacterial suspension, diluted 1,000 times is still better than that of 24% fenbuconazole suspension. The applying bacterial suspension enables reliable control of brown rot in D. latiflorus.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.1
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• pp.120-126
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• 2020

Objective: This study was conducted to determine the effects of dietary supplementation with Pediococcus acidilactici (P. acidilactici) ZPA017 as a probiotic on reproductive performance, fecal microbial flora and serum indices in sows during late gestation and lactation. Methods: A total of 94 sows (Large White×Yorkshire, average 4.50 parities) were randomly allotted to two dietary treatments: control diet and the diet supplemented with P. acidilactici ZPA017 (2.40×10⁹ colony-forming unit/kg of diets). The study started at d 90 of gestation and conducted until d 28 of lactation. Results: Compared to sows fed the control diet, supplementation of P. acidilactici ZPA017 increased the number of weaning piglets, weaning weight of litter and piglets, survival rate of piglets at weaning (p<0.05), and decreased diarrhea rate of piglets in lactation (p<0.05). Dietary P. acidilactici ZPA017 increased fecal Lactobacillus populations (p = 0.030) and reduced fecal Escherichia coli and Staphylococcus aureus populations (p<0.05) of sows at weaning. Moreover, the supplementation of P. acidilactici ZPA017 increased serum concentrations of immunoglobulin G, immunoglobulin A and total protein (p<0.05), while decreased serum haptoglobin concentration and alanine aminotransferase activity (p<0.05) of sows at weaning. Conclusion: Administration of P. acidilactici ZPA017 in diets during late gestation and lactation had positive effects on the reproductive performance, intestinal microflora balance and immunity of sows.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.20 no.4
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• pp.405-413
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• 2002

It is crucial that we should set proper standards capable of efficiently handling new corrections of maps, connections of edges on the maps, administrative districts, and inter-scale connections in order to make serial cadastral maps. This study drew a two dimensional Parameter using an indent point, as a Review Control Point, on forest screening line or boundary line of administrative district. The study also introduced a few different transformations such as Affine Transformation, N-Degree Polynomial Transformation, and Projective Transformation, the two dimensional transformation methods to apply them to the connection of cadastal·forestry maps and the connection of parcels between the administrative districts, on the forestry map designed by discretionary edges on the maps per district unit with parcel numbers and tried to apply the coordinates transformation method to connections of maps to make serial cadastral maps.

• Food Science of Animal Resources
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• v.38 no.2
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• pp.376-390
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• 2018

Cronobacter species have been associated with disease outbreaks and sporadic infections, particularly in premature and immunocompromised infants. Cronobacter species can cause foodborne infections such as neonatal meningitis, septicaemia and necrotising enterocolitis. Accordingly, there is an urgent need to control and monitor the Cronobacter species in food, especially in powdered infant formula (PIF) and other baby foods. Therefore, in this review, the isolation and prevalence of Cronobacter species in infant food including PIF and the recent advance of detection methods are discussed for the better understanding on the current research status of Cronobacter species.

• Korean Journal of Agricultural Science
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• v.43 no.3
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• pp.299-319
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• 2016

The National Forest Inventory (NFI) is important for providing fundamental data for basic forest planning and the establishment of forest policies for the purpose of implementing sustainable forest management. The purpose of this study is to present the development of Korea's NFI including legal basis, sampling design, and measured variables and to review the usage of NFI data. The survey methods and forestry statistics among the Unites States, Canada, Japan, China, and European countries were briefly compared. Total 140 publications utilizing NFI data between 2008 and 2015 were categorized with 15 subjects. Korea has conducted the NFI 6 times since 1971, but only the $6^{th}$ NFI is comparable with the fifth, the previous NFI, because the permanent sampling plots have been shared between the periods. The Korean Forestry Statistics contains only half as many variables as that of advanced countries in Forestry. More researches were needed to improve consistent measurement of diverse variables through implementation of advanced technologies. Additional data for Forest Health Monitoring since the NFI $6^{th}$ must be under quality control which will be an essential part of the inventories for providing the chronological change of forest health.

• Smart Structures and Systems
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• v.30 no.1
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• pp.75-88
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• 2022

Engineering structures in operation essentially belong to time-varying or nonlinear structures and the resultant response signals are usually non-stationary. For such time-varying structures, it is of great importance to extract time-dependent dynamic parameters from non-stationary response signals, which benefits structural health monitoring, safety assessment and vibration control. However, various traditional signal processing methods are unable to extract the embedded meaningful information. As a newly developed technique, variational mode decomposition (VMD) shows its superiority on signal decomposition, however, it still suffers two main problems. The foremost problem is that the number of modal components is required to be defined in advance. Another problem needs to be addressed is that VMD cannot effectively separate non-stationary signals composed of closely spaced or overlapped modes. As such, a new method named generalized adaptive variational modal decomposition (GAVMD) is proposed. In this new method, the number of component signals is adaptively estimated by an index of mean frequency, while the generalized demodulation algorithm is introduced to yield a generalized VMD that can decompose mode overlapped signals successfully. After that, synchrosqueezing wavelet transform (SWT) is applied to extract instantaneous frequencies (IFs) of the decomposed mono-component signals. To verify the validity and accuracy of the proposed method, three numerical examples and a steel cable with time-varying tension force are investigated. The results demonstrate that the proposed GAVMD method can decompose the multi-component signal with overlapped modes well and its combination with SWT enables a successful IF extraction of each individual component.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.1613-1613
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• 2001

Fats and molasses are used, at the present time, in a considerable proportion as ingredients for the animal feed industry. They are mainly used as energy sources, but also they provide other characteristics of technological and nutritional interest (dust reduction, increase in palatability, etc). Both semi-liquid ingredients have numerous aspects in common from the point of view of their use in livestock feeds, as well as of their analytical control. Feed manufacturers use several criteria to evaluate the quality of fat and molasses. Furthermore, the traditional methods currently used, for their evaluation (eg. fatty acids, sugars, etc) are expensive and more sophisticated that the traditionally used for solid ingredients. The objective of the present work is to carry out a viability study to evaluate the ability of NIRS technology for the quality control of fat and molasses. Samples of liquid molasses (n = 42) and liquid fat ( n = 61), provided by a feed manufacturer, were scanned in a FOSS-NIR Systems 6500 monochromator equipped with a spinning module. The samples were analysed by folded transmission, using a sample cup of 0.1mm pathlength and gold surface reflector. For molasses, calibration equations were developed for the prediction of moisture (SECV=1.69%; $r^2$=0, 42), gross protein (SECV=0, 14%; $r^2$=0, 99), ashy (SECV=0, 60%; $r^2$=0, 84), NaCl (SECV=0, 05%; $r^2$=0, 99) and sugars (SECV=1, 04%; $r^2$=0, 86). For animal fats calibrations were obtained for the prediction of moisture (SECV=0, 14%, $r^2$=0, 88), acidity index (SECV=0, 83%, $r^2$=0, 82), MIU (SECV=0, 38%, $r^2$=0, 94) and unsaponifiables (SECV=0, 45%, $r^2$=0, 87). High accuracy calibration equations were also obtained for the prediction of the fatty acid profile. The equations have $r^2$values around 0.9 or highest. The results showed that NIRS technology could provide rapid and accurate results and reduce analytical costs associated to the quality control of two Important feed ingredients of a well known chemical variability.

• Journal of Korean Society of Forest Science
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• v.100 no.2
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• pp.165-171
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• 2011

This study was conducted to investigate the effect on pine wilt disease by health-thinning, thinning and sapling tending in red pine forest (Pinus densiflora). As a part of developing forestry control methods for pine wilt disease control. In case of putting in pine sawyer (Monochamus alternatus) with pine wood nematode (Bursaphelenchus xylophilus), the specimen trees in health-thinning, thinning and control treatment were withered more than 50%, although there were not statistically significant differences in treatments. In treatment site, thinning slashes had influenced on the spread of pine wilt disease (experiment 1). In thinning stand of sapling pine, site with thinning slashes had highest mortality (> 90%). There were approximately 10% mortality in the site of carried thinning slashes from case and the site of non-thinning with released M. alternatus (experiment 2). The larvae had not appeared in young tree stump with health-thinning and thinning at April, the current emergence year of M. alternatus, but there were larvae in sapling tree stump with thinning at May, the current emergence year of M. alternatus. In case of stands with infected young and sapling tree by pine wilt disease, there is no effects of on pine wilt disease control by health-thinning, thinning, saplings tending at April and May, the current emergence year of M. alternatus, and leaved thinning slashes had influenced on the spread of pine wilt disease as habitation of M. alternatus.