• Journal of Microbiology and Biotechnology
• /
• v.23 no.6
• /
• pp.864-871
• /
• 2013

The recombinant strain P. pastoris GS115-lccC was used to produce laccase with high activity. Factors influencing laccase expression, such as pH, methanol concentration, copper concentration, peptone concentration, shaker rotate speed, and medium volume were investigated. Under the optimal conditions, laccase activity reached 12,344 U/L on day 15. The recombinant enzyme was purified by precipitating and dialyzing to electrophoretic homogeneity, and was estimated to have a molecular mass of about 58 kDa. When guaiacol was the substrate, the laccase showed the highest activity at pH 5.0 and was stable when the pH was 4.5~6.0. The optimal temperature for the laccase to oxidize guaiacol was $60^{\circ}C$, but it was not stable at high temperature. The enzyme could remain stable at $30^{\circ}C$ for 5 days. The recombinant laccase was used to degrade chlorpyrifos in several laccase/mediator systems. Among three synthetic mediators (ABTS, HBT, VA) and three natural mediators (vanillin, 2,6-DMP, and guaiacol), vanillin showed the most enhancement on degradation of chlorpyrifos. Both laccase and vanillin were responsible for the degradation of chlorpyrifos. A higher dosage of vanillin may promote a higher level of degradation of chlorpyrifos, and the 2-step addition of vanillin led to 98% chlorpyrifos degradation. The degradation of chlorpyrifos was faster in the L/V system ($k_{obs}$ = 0.151) than that in the buffer solution ($k_{obs}$ = 0.028).

• Journal of the Korean Wood Science and Technology
• /
• v.47 no.5
• /
• pp.587-596
• /
• 2019

This study aimed to determine the antimalarial effect of the Strychnos ligustrina (SLW) wood extracts and to analyze its phytochemicals. The SLW powder samples were macerated with 100% ethanol (E100), 75% ethanol (E75), 50% ethanol (E50), 25% ethanol (E25), and aqueous (A100). The extracts were analyzed by LCMS/MS, and its in-vitro antimalarial activity was tested with Plasmodium falciparum. The results showed that the extract yields of E100, E75, E50, E25, and A100 were 4.3, 5.2, 5.3, 4.7, and 3.6%, respectively. The antimalarial activities of the A100, E25, E50, and E75 extracts were classified as active with $IC_{50}$ values of 38.6, 42.6, 42.9, and $43.7{\mu}g/mL$, respectively. But, the antimalarial activity of the E100 extract was classified as slightly active with $IC_{50}$ values of $87.4{\mu}g/mL$. The dominant compounds contained in the extracts of A100, E25, E50, E75, and E100 was the alkaloid compound, namely brucine with relative concentrations of 24.96, 24.55, 21.33, 11.79, and 11.62%, respectively.

• Journal of Forest and Environmental Science
• /
• v.29 no.1
• /
• pp.71-80
• /
• 2013

Present study analyzes the rooftop gardening status, floristic composition and cost and return of the rooftop garden in Sylhet City Corporation of northeastern Bangladesh. Data was collected from 450 rooftop gardeners randomly during July-September 2010. Study reveal that rooftop gardening is generally for mental satisfaction (95.3%) followed by leisure time activity (87.8%) in the study area and almost all the family members of gardeners' were involved; while collection of planting materials, sites preparation and marketing of products were reported to be carried out by males only (male 71.33%). Middle income classes were most interested in rooftop gardening (43.78%). The survey recorded 53 plant species (35 families) of which Cucurbitaceae family represented highest eight species. Shrubs (28%) were highest followed by herbs (26%) among agri-crops (36%) and flower species (30%). About 89% of the rooftop gardeners procured planting materials from nursery, market, fair, neighbor, relative and friends and they mostly prefer to use seedlings (48%) for roof gardening followed by direct seed sowing (21%). Gardeners sell products sporadically in different local markets, directly or through intermediaries, with no uniform pricing for system. Rooftop gardening improves the food security and meet nutritional deficiency to the gardeners. Survey revealed that generally very few people consider rooftop gardening commercially to get profit and from the cost-return analysis this gardening system can be economically viable if proper and scientifically managed. The study conclude that active government and NGOs could play vital role to increasing this activities by providing training and motivate people with technical aspects of rooftop gardening.

• Journal of Korean Society of Forest Science
• /
• v.76 no.3
• /
• pp.243-248
• /
• 1987

The U.S. Forest Service administers the world's largest forestry research organization. From its modest beginning in 1876, some 30 years before the United States national forest system was established, the research branch has devoted its effort to meet current and future information needs of the forestry community of the United States, not just for the U.S. Forest Service. The research branch is one of three major administrative units of the U.S. Forest Service. The others being the National Forest System and State and Private Forestry. Currently the National Forest System comprises 155 national forests, 19 national grasslands, and 18 utilization projects located in 44 states. Puerto Rico, and the Virgin Islands. The National Forest System manages these areas for a large array of uses and benefits including timber, water, forage, wildlife, recreation, minerals, and wilderness. It is through the State and Private Forestry branch that the U.S. Forest Service cooperates and coordinates forestry activities and programs with state and local governments, forest industries, and private landowners. These activities include financial and technical assistance in disease, insect, and fire protection ; plan forestry programs ; improve harvesting and marketing practices ; and transfer forestry research results to user groups. Forestry research is carried out through eight regional Forest Experiment Stations and the Forest Product Laboratory. Studies are maintained at 70 administrative sites, and at 115 experimental forest and grasslands. All of the current sciences that composed modern forestry are included in the research program. These range from forest biology (i. e. silviculture, ecology, physiology, and genetics) to the physical, mathematical, engineering, managerial, and social sciences. The levels of research range from application, developmental, and basic research. Research planning and priority identification is an ongoing process with elements of the research program changing to meet short-term critical information needs(i. e. protection research) to long-term opportunities(i. e. biotechnology). Research planning and priority setting is done in cooperation with National Forest Systems, forest industries, universities, and individual groups such as environmental, wilderness, or wildlife organizations. There is an ongoing review process of research administration, organization, and science content to maintain quality of research. In the U.S. Forest Service the research responsibility is not completed until the new information is being applied by the various user group : I. e. technology transfer program. Research planning and development in the U.S. Forest Service is a dynamic activity. Porgrams for the year 2000 and beyond are now in the planning stage.

• Asian-Australasian Journal of Animal Sciences
• /
• v.28 no.12
• /
• pp.1703-1712
• /
• 2015

The in vitro maturation (IVM) efficiency of porcine embryos is still low because of poor oocyte quality. Although brilliant cresyl blue positive (BCB+) oocytes with low glucose-6-phosphate dehydrogenase (G6PDH) activity have shown superior quality than BCB negative (-) oocytes with high G6PDH activity, the use of a BCB staining test before IVM is still controversial. This study aimed to shed more light on the subcellular characteristics of porcine oocytes after selection using BCB staining. We assessed germinal vesicle chromatin configuration, cortical granule (CG) migration, mitochondrial distribution, the levels of acetylated lysine 9 of histone H3 (AcH3K9) and nuclear apoptosis features to investigate the correlation between G6PDH activity and these developmentally related features. A pattern of chromatin surrounding the nucleoli was seen in 53.0% of BCB+ oocytes and 77.6% of BCB+ oocytes showed peripherally distributed CGs. After IVM, 48.7% of BCB+ oocytes had a diffused mitochondrial distribution pattern. However, there were no significant differences in the levels of AcH3K9 in the nuclei of blastocysts derived from BCB+ and BCB- oocytes; at the same time, we observed a similar incidence of apoptosis in the BCB+ and control groups. Although this study indicated that G6PDH activity in porcine oocytes was correlated with several subcellular characteristics such as germinal vesicle chromatin configuration, CG migration and mitochondrial distribution, other features such as AcH3K9 level and nuclear apoptotic features were not associated with G6PDH activity and did not validate the BCB staining test. In using this test for selecting porcine oocytes, subcellular characteristics such as the AcH3K9 level and apoptotic nuclear features should also be considered. Adding histone deacetylase inhibitors or apoptosis inhibitors into the culture medium used might improve the efficiency of IVM of BCB+ oocytes.

• Journal of Forest and Environmental Science
• /
• v.33 no.1
• /
• pp.8-21
• /
• 2017

The objective of this study is to identify the individual characteristics and the elements of social capital hence a suitable design of social capital reinforcement can be proposed to promote a sustainable community-based mangrove management. The research conducted in three villages that were selected based on mangrove ecological differences in Lampung Province, Indonesia. Qualitative data was collected through field observation and in-depth interviews with key informants. The results showed that the population in the three villages dominated on productive ages, worked as farmers, and less educated (only a half of the population had fulfilled the basic education standard of Indonesia). The study results also indicated that the social capital in the communities showed minimum condition of interpersonal attachment and cooperation. This condition was derived from the attitude of the people who only took personal benefit without concerning to others' welfare. Many programs conducted by government to ensure the conservation of mangroves were project-oriented with minor participation of community. The minor participation might also contributed to the minimum of social capital in the community. To improve social capital, the communities should strengthen mutual trust based on mutual benefit to increase members' participation in mangrove activity.

• Journal of the Korean Wood Science and Technology
• /
• v.48 no.5
• /
• pp.641-650
• /
• 2020

In this study, the chemical components and termiticidal activities of bamboo vinegar against subterranean termite were evaluated. Bamboo vinegar used in this study were produced from Mayan (Gigantochloa robusta Kurz.), Balcoa (Bambusa balcooa Roxb.), and Taiwan (Dendrocalamus latiflorus Munro). It was analyzed by gravimetric methods. Rubberwood (Hevea brasiliensis) was soaked for 24 h and tested against Coptotermes curvignathus Holmgren according to the Indonesian National Standard (SNI 7207-2014). The bamboo vinegar demonstrated antitermite activities against subterranean termite. Vinegar of 400℃ recorded higher total phenol and acid than that of 300℃. Results from this study suggest that the phenolic and acidic component of the bamboo vinegar contributed to 100% mortality rate of the termite, some wood weight loss, and a decrease in the termite feeding rate. These results strongly recommend that bamboo vinegar from D. latiflorus Munro is a potential environmentally friendly preservative.

• Journal of Microbiology and Biotechnology
• /
• v.31 no.7
• /
• pp.978-989
• /
• 2021

Allyl isothiocyanate (AITC), as a fumigant, plays an important role in soil control of nematodes, soil-borne pathogens, and weeds, but its effects on soil microorganisms are unclear. In this study, the effects of AITC on microbial diversity and community composition of Capsicum annuum L. soil were investigated through Illumina high-throughput sequencing. The results showed that microbial diversity and community structure were significantly influenced by AITC. AITC reduced the diversity of soil bacteria, stimulated the diversity of the soil fungal community, and significantly changed the structure of fungal community. AITC decreased the relative abundance of dominant bacteria Planctomycetes, Acinetobacter, Pseudodeganella, and RB41, but increased that of Lysobacter, Sphingomonas, Pseudomonas, Luteimonas, Pseudoxanthomonas, and Bacillus at the genera level, while for fungi, Trichoderma, Neurospora, and Lasiodiplodia decreased significantly and Aspergillus, Cladosporium, Fusarium, Penicillium, and Saccharomyces were higher than the control. The correlation analysis suggested cellulase had a significant correlation with fungal operational taxonomic units and there was a significant correlation between cellulase and fungal diversity, while catalase, cellulose, sucrase, and urease were the major contributors in the shift of the community structure. Our results will provide useful information for the use of AITC in the assessment of environmental and ecological security.

• Journal of Practical Agriculture & Fisheries Research
• /
• v.24 no.2
• /
• pp.5-11
• /
• 2022

Prunus × yedoensis is a representative tree species in residential areas often used as a street tree or park tree, with its popularity due to splendid flowers in the spring. We investigated plant growth, photosynthesis, chlorophyll, and carotenoid concentrations of Prunus yedoensis at 400ml/pot, 1,000ml/pot, and 2,000ml/pot Irrigation treatments. Overall growth indices such as tree height, diameter at root collar (DRC), and total dry weight could be ranked in the decreasing order "2,000ml/pot > 1,000ml/pot > 400ml/pot.". However, 2,000ml/pot featured decreased photosynthesis and total chlorophyll carotenoid contents than 1,000ml/pot treatment after July. And also, The 1,000ml/pot treatment showed relatively high photosynthetic activity and chlorophyll contents. These results suggested that the cultivation of Prunus yedoensis under 1,000ml/pot Irrigation treatment(soil water contents 25~35%) showed the best condition in growth.

• Journal of Korean Society of Rural Planning
• /
• v.26 no.2
• /
• pp.83-93
• /
• 2020

Rural areas have been faced with various problems such as aging and population decline, and in these circumstances, the activation of local communities is suggested as a solution. Agricultural producer organizations are a powerful means of maintaining rural society. The purpose of this study is to understand the characteristics of agricultural community activities by region by comparing the status of organization and activities and analyzing Location Quotient(LQ) based on the regional survey data in the 2015 census of agriculture, forestry, and fisheries. As the results of the analysis, it was found that 38.35% of villages participated in economic organizations and 32.22% of villages were having urban-rural exchange activities. The crop clubs were actively operated in the Gyeongsangbuk province, and other economic organizations were organized in Jeju, Haenam-gun, and Dangjin-si more than other regions. And the urban-rural exchange activities were found in Chungcheongnam province mainly. The results of detailed LQ analysis by organization and activity showed that specialized regions for each community or activity were different. These results could be used for regional planning or the establishment of strategies for community activation.