• 제목/요약/키워드: forensic sciences

검색결과 243건 처리시간 0.024초

Application study of PCR additives to improve the split peaks in direct PCR

  • Kim, Joo-Young;Kim, Da-Hye;Park, Hyun-Chul;Jung, Ju Yeon;Jin, Gang-Nam;Hwang, In-Kwan;Kang, Pil-Won
    • 분석과학
    • /
    • 제32권4호
    • /
    • pp.155-162
    • /
    • 2019
  • Analysis techniques using DNA profiling are widely used in various fields including forensic science and new technologies such as the Direct PCR amplification method are being developed continuously in order to acquire the DNA profiles efficiently. However, it has a limits such as non-specific amplification according to the quality of crime scene evidence samples. Especially, split peaks caused by excessive DNA samples are one of the important factors that could cause the debate to allow researchers to interpret the DNA profile results. In this study, we confirmed the occurrence rate of split peaks in each STR (short tandem repeats) locus of the $GlobalFiler^{TM}$ kit and investigated the possibility of improving the split peaks using several PCR additives such as DMSO (dimethylsulfoxide), $MgCl_2$, Betaine and Tween-20. As a result, we could make three groups according to the occurrence rate of split peaks in Direct PCR and it was confirmed that the ratio of split peaks could be reduced by DMSO (87.4 %), $MgCl_2$ (84.5 %) and Betaine (86.1 %), respectively. These results indicate that PCR additives such as DMSO, $MgCl_2$ and Betaine can be improve the split peaks in Direct PCR and thereby facilitate subsequently a successful DNA profile results.

안드로이드 스마트폰을 위한 앤티-포렌식 도구들의 활용성 (On the Availability of Anti-Forensic Tools for Android Smartphones)

  • 문필주
    • 한국전자통신학회논문지
    • /
    • 제8권6호
    • /
    • pp.855-861
    • /
    • 2013
  • 스마트폰은 컴퓨팅 능력 향상, 전송속도의 고속화, 다양한 애플리케이션의 활용 등을 통해 실생활에 매우 유익하게 사용되고 있다. 반면에 스마트폰을 이용한 범죄가 증가하고 있으며, 앤티-포렌식 도구들을 사용하여 스마트폰 메모리의 데이터를 고의로 삭제하는 경우도 발생하고 있다. 본 논문에서는 안드로이드 스마트폰 상에서 사용되는 앤티-포렌식 도구들을 조사 및 분석하여 앤티-포렌식 도구들의 특성과 기법을 연구한다. 또한, 상용 포렌식 도구인 Oxygen Forensic Suite를 이용하여 앤티-포렌식 도구들이 제공하는 기능들의 활용성을 검증하는 실험을 실시한다.

A Case of Single-step Mutations at Two Short Tandem Repeat loci (D13S317 and DXS10148) among Three Generations of a Korean Family

  • Byeong Ju Youn;Kyungmyung Lee;Cho Hee Kim
    • 대한의생명과학회지
    • /
    • 제28권4호
    • /
    • pp.327-333
    • /
    • 2022
  • The DNA profiling of short tandem repeat (STR) markers is a powerful tool for forensic identification and forensic paternity testing. However, STR loci are susceptible to mutation that cause mismatches between parents and children when paternity is tested. Herein, we examined paternity disputes with 23 autosomal STR loci using two commercial human identification kits and revealed successive mismatches at the D13S317 locus across three generations of a Korean family. Additionally, we investigated 12 X-chromosomal STRs and discovered an inconsistency at the DXS10148 locus between the father and daughter of the same Korean family. Furthermore, we confirmed STR genotypes at the D13S317 and DXS10148 loci of the family using sequencing analysis. Consequently, we identified a successive single-step mutation at the D13S317 locus and one single-step mutation at the DXS10148 locus in three generations of the Korean family. Therefore, this case study may be useful for interpreting and understanding forensic paternity tests.

Evaluation of Rapid IgG4 Test for Diagnosis of Gnathostomiasis

  • Wang, Yue;Ma, An;Liu, Xiao-Long;Eamsobhana, Praphathip;Gan, Xiao-Xian
    • Parasites, Hosts and Diseases
    • /
    • 제59권3호
    • /
    • pp.257-263
    • /
    • 2021
  • Human gnathostomiasis is a parasitic disease caused by Gnathostoma nematode infection. A rapid, reliable, and practical immunoassay, named dot immuno-gold filtration assay (DIGFA), was developed to supporting clinical diagnosis of gnathostomiasis. The practical tool detected anti-Gnathostoma-specific IgG4 in human serum using crude extract of third-stage larvae as antigen. The result of the test was shown by anti-human IgG4 monoclonal antibody conjugated colloidal gold. The sensitivity and specificity of the test were both 100% for detection in human sera from patients with gnathostomiasis (13/13) and from healthy negative controls (50/50), respectively. Cross-reactivity with heterogonous serum samples from patients with other helminthiases ranged from 0 (trichinosis, paragonimiasis, clonorchiasis, schistosomiasis, and cysticercosis) to 25.0% (sparganosis), with an average of 6.3% (7/112). Moreover, specific IgG4 antibodies diminished at 6 months after treatment. This study showed that DIGFA for the detection of specific IgG4 in human sera could be a promising tool for the diagnosis of gnathostomiasis and useful for evaluating therapeutic effects.

과학수사용 화자 식별 시스템의 피치 차이에 따른 신뢰성 척도 (Confidence Measure of Forensic Speaker Identification System According to Pitch Variances)

  • 김민석;김경화;양일호;유하진
    • 말소리와 음성과학
    • /
    • 제2권3호
    • /
    • pp.135-139
    • /
    • 2010
  • Forensic speaker identification needs high accuracy and reliability. However, the current level of speaker identification does not reach its demand. Therefore, the confidence evaluation of results is one of the issues in forensic speaker identification. In this paper, we propose a new confidence measure of forensic speaker identification system. This is based on pitch differences between the registered utterances of the identified speaker and the test utterance. In the experiments, we evaluate this confidence measure by speech identification tasks on various environments. As the results, the proposed measure can be a good measure indicating if the result is reliable or not.

  • PDF

Effectiveness of interference filter for photoluminescence observations: comparison with absorption filters

  • Lee, Wonyoung;Kim, Duke;Yu, Jeseol
    • 분석과학
    • /
    • 제34권5호
    • /
    • pp.225-230
    • /
    • 2021
  • Currently, most photoluminescence reagents used in forensic science have short stokes shift, making it difficult to observe strong photoluminescence due to scattering light from forensic light sources when using filters that are not shieldable. However interference filters can be observed near monochromatic light, making them a better alternative to absorption filters. To verify practical applicability of interference filters, interference filters and three other types of previously frequently used absorption filters were observed using a variety of light source products, and transmission light was cross-compared. Interference filters have a lower slope value than absorption filters, and selectively show only the photoluminescence of reagents, regardless of the type of product from the forensic light source. In addition, tilting the angle of the filter surface for observation lowered the λcut-on, which could replace various types of absorption filters with a single interference filter.

Smart Phone OS별 포렌식 기술과 도구에 관한 연구 (A Study of Forensic Techniques and Tools on Smart Phone OS Types)

  • 이보만;박대우
    • 한국정보통신학회:학술대회논문집
    • /
    • 한국해양정보통신학회 2010년도 추계학술대회
    • /
    • pp.223-226
    • /
    • 2010
  • iPhone과 갤럭시S의 국내 출시와 함께 Smart Phone의 국내 시장이 활성화 되면서 사용자들이 증가하고 있다. 이에 따라 기존의 휴대폰 관련 범죄 증거 자료 수집을 위한 포렌식 기법으로는 새 기술과 WiFi 등 Convergence된 Smart Phone에 대한 모바일 포렌식 기술 연구가 필요하다. 본 논문에서는 Smart Phone OS별 포렌식 기술과 도구 연구를 위해, iPhone Apple MAC OS X와 갤럭시S Google Android를 연구한다. Smart Phone OS별 포렌식 기술로는 SYN 방식을 이용하여 포렌식 기술을 연구하고, 모바일 포렌식을 하기 위해 컴퓨터 포렌식 방법과 도구들을 살펴보고 포렌식 적용 방법을 연구한다. 본 논문 연구가 IT 강국으로서 한국의 Smart Phone 포렌식 기술 발전에 기여할 것이다.

  • PDF

스마트폰 모바일 포렌식 증거 수집 분석을 위한 준비사항 및 절차 연구 (A study of the preparation And procedures by Smartphone Mobile Forensic evidence collection and analysis)

  • 이재현;박대우
    • 한국정보통신학회:학술대회논문집
    • /
    • 한국해양정보통신학회 2011년도 추계학술대회
    • /
    • pp.269-272
    • /
    • 2011
  • 스마트폰에 대한 소송이 이루어지고 있고, 최근, 법정에서의 스마트폰 증거데이터에 대한 증거자료 채택이 많아지고 있다. 따라서 불법적인 스마트폰 사용에 대한 증거데이터 추출을 위한 포렌식 절차와 증거물 수집에 대한 연구가 필요하다. 본 논문에서는 스마트폰의 증거데이터 주출에 대한 포렌식 절차를 제시하고, 스마트폰 포렌식 증거를 수집함으로써 디지털 증거의 무결성을 확보하고 사건을 진실을 발견하기 위한 방법에 대해 연구하였다. 본 연구를 통해 스마트폰 포렌식의 발전에 기여할 수 있을 것이다.

  • PDF

Script에서 Forensic 증거자료 확보 방안 (Generation of Forensic Evidence Data from Script)

  • 김슬기;박대우
    • 한국정보통신학회:학술대회논문집
    • /
    • 한국정보통신학회 2017년도 추계학술대회
    • /
    • pp.353-355
    • /
    • 2017
  • 최근 발달된 디지털기기들은 사이버로 연결되어 사용되고 있다. 디지털기기 사용자들은 사이버에 연결된 단말기들을 활용하여 금융결제, 전자상거래 등 활동을 하고 있다. 사이버거래의 활성화와 함께 사용자에 대한 사이버범죄가 증가하고 있다. 사이버범죄의 수사에서 Forensic 증거자료를 확보하여야 한다. 하지만, 디지털 포렌식 증거자료를 분석하기에는 많은 정보가 함유되어 있다. 이러한 많은 디지털정보에서, 사이버범죄에 증거를 확보하기 위한 Script가 효율적인 방안이다. 본 논문 연구에서는 Script를 활용하여, Forensic 증거자료를 확보하는 방안을 연구한다. EnCase를 통한 증거자료 추출과 Script를 활용한 증거자료 확보 방안을 연구한다. 본 연구는 안전한 국민생활을 위한 사이버 보안의 기초자료로 활용될 것이다.

  • PDF

Digital Forensics Investigation Approaches in Mitigating Cybercrimes: A Review

  • Abdullahi Aminu, Kazaure;Aman Jantan;Mohd Najwadi Yusoff
    • Journal of Information Science Theory and Practice
    • /
    • 제11권4호
    • /
    • pp.14-39
    • /
    • 2023
  • Cybercrime is a significant threat to Internet users, involving crimes committed using computers or computer networks. The landscape of cyberspace presents a complex terrain, making the task of tracing the origins of sensitive data a formidable and often elusive endeavor. However, tracing the source of sensitive data in online cyberspace is critically challenging, and detecting cyber-criminals on the other hand remains a time-consuming process, especially in social networks. Cyber-criminals target individuals for financial gain or to cause harm to their assets, resulting in the loss or theft of millions of user data over the past few decades. Forensic professionals play a vital role in conducting successful investigations and acquiring legally acceptable evidence admissible in court proceedings using modern techniques. This study aims to provide an overview of forensic investigation methods for extracting digital evidence from computer systems and mobile devices to combat persistent cybercrime. It also discusses current cybercrime issues and mitigation procedures.