• 분석과학
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• 제35권6호
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• pp.242-248
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• 2022

Luminol 혹은 Bluestar가 혈액 및 표백제와 반응할 때 ethylenediamine (EDA)이 미치는 영향에 대해 연구하였다. 이를 위해 거름종이에 혈액, 염소계 표백제, 산소계 표백제를 묻힌 후 EDA를 첨가한 luminol 혹은 Bluestar로 처리한 후 화학발광의 세기 변화를 관찰하였다. 그 결과 EDA의 농도를 증가시켜도 luminol (혹은 Bluestar)-혈액 반응의 화학발광 세기는 변하지 않는다는 것을 알 수 있었다. 그러나 EDA의 농도가 증가할수록 luminol (혹은 Bluestar)-염소계 표백제 반응의 화학발광 세기는 감소하였고, luminol (혹은 Bluestar)-산소계 표백제 반응의 화학발광 세기는 증가하는 현상이 관찰되었다. 이를 통해 luminol (혹은 Bluestar)로 혈액 검출 시약을 제조할 때 EDA를 첨가할 경우, EDA는 염소계 표백제에 의해 유발되는 위양성 반응을 억제하고 산소계 표백제의 위양성 반응을 유발한다는 것을 알 수 있었다. 이런 점을 종합해서 판단할때 luminol이나 Bluestar로 혈액을증강할 때 EDA를 첨가하는것은 적절하지 않다.

• 분석과학
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• 제29권2호
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• pp.73-78
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• 2016

시안화수소는 ABS (acrylonitrile-butadiene-styrene) 등의 플라스틱류 화재시에 발생될 수 있는 유독성 기체이기에 시안화수소가 함유된 연기를 흡입하였을 경우 변사자의 혈액에서도 검출될 수 있다. 따라서 열기로 혈액 등의 생체시료의 손상정도가 강하여 일산화탄소-헤모글로빈 농도의 측정이 어려운 경우 사인규명을 위한 지표로 활용될 수 있으리라 사료된다. 본 연구의 사례는 유서와 함께 화재가 발생한 차량내에서 발견된 변사체의 사인규명과 관련이 있다. 화재사의 경우 일산화탄소-헤모글로빈 농도가 결정적인 정보를 제공하지만 열기로 심하게 손상되어 이에 대한 측정이 어려워 시안화이온을 분석하고자 하였다. 시안화이온을 양이온계면활성제와 함께 pentafluorobenzyl bromide로 유도체화 시킨 다음 기체크로마토그래피/질량분석법으로 scan mode와 SIM mode로 분석하였다. 변사체 심혈과 뇌혈에서 시안화이온이 정상 흡연자 14인의 평균값(0.041 µg/mL)보다 높은 0.36 µg/mL 및 1.20 µg/mL 확인되었다.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2010년도 추계학술대회
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• pp.215-218
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• 2010

국내 Smart Phone 수요가 급속도로 증가함에 따라 멀티미디어 서비스의 활용도 다양해졌다. 이에 따라 Smart Phone 사용자들은 Jail Breaking과 Rooting 등 해킹을 하여 멀티미디어 저작권 콘텐츠를 불법으로 이용하고 있다. 또한 이동통신 단말로서 범죄와 관련성이 높아서, 생성, 저장된 디지털 증거는 증거의 활용도가 높아 모바일 포렌식 연구가 필요하다. 본 논문은 Smart Phone 저작권 위반과 포렌식 적용 방안에 대한 연구이다. Smart Phone 저작권 침해 현황과 관련 위반사항들을 방송, 영화, 음악, e-book 등으로 항목별로 조사하고, 포렌식에 적용 하는 방법에 대해 연구하였다. 본 연구 결과는 과학수사 발전과 포렌식 발전에 기여 할 수 있을 것이다.

• 중환자간호학회지
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• 제13권3호
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• pp.24-40
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• 2020

Purpose : This study aims to develop the Forensic Nursing Competency Scale suitable to South Korea. Methods : The initial items were generated through literature review and interviews. Items were evaluated by experts for content and face validity. By conducting a pilot test, 74 measurement items were developed. In total, 333 copies of the questionnaire were analyzed regarding the validity and reliability of the study. Data were analyzed using SPSS 23.0 program and AMOS 18.0 program. Results : The study selected 53 questions after testing the content, face, and construct validity of the scale. The final 53 questions were composed of 15 sub-components in eight domains. These eight domains encompassed nursing practice, communication, critical thinking, human caring and relationships, evidence management, active information collection, professional development, and knowledge integration. Conclusions : In order to verify the effectiveness of the scale, future studies need to compare the forensic nursing competency in two groups to assess differences in these competency. Moreover, it is necessary to develop a forensic nursing educational program and validate its effectiveness by using this scale.

• 분석과학
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• 제30권2호
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• pp.75-81
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• 2017

5-methylthioninhydrin (5-MTN) is an amino acid sensitive reagent used for the development of latent fingermarks deposited on porous surfaces such as paper and wood. The present study demonstrates that the 5-MTN can be used as a latent footwear impression enhancement reagent, by reacting with trace multivalent metal ions, which are the main components of the latent footwear impression. 5-MTN and L-alanine complex (MTN-ALA) used for the latent footwear impression development was prepared, by mixing $4.5{\times}10^{-3}M$ 5-MTN (in methanol) and $4.5{\times}10^{-3}M$ L-alanine (in methanol) in 1:1 ratio, and keeping undisturbed at room temperature for 24 h. The latent footwear impressions were deposited on white and black non-porous surfaces (glass plate, polyethylene panel, polypropylene panel, acryl panel, polyvinyl chloride (PVC) panel, poly(methyl methacrylate) (PMMA) panel, acrylonitrile-butadiene-styrene (ABS) panel, tile), and a semi-porous surfaces (painted wood). The latent footwear impressions on these surfaces were treated with MTN-ALA complex by spraying. The fluorescence of footwear impressions (occurred due to the reaction between MTN-ALA and metal complexes) was observed under a 505 nm forensic light source and an orange barrier filter. The enhancement of latent footwear impression was achieved from black surfaces without any blurring. However, the fluorescence (enhancement) of footwear impression was not observed on the white PVC, PMMA, and ABS surfaces, because the incident light interfered and reflected on the surface. The sensitivity of MTN-ALA was superior to 2,2'-dipyridil, which is a representative non-fluorescing footwear impression enhancement reagent, and similar to 8-hydroxyquinoline, which is a representative fluorescing footwear impression enhancement reagent.

• 분석과학
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• 제36권6호
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• pp.324-331
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• 2023

젖은 종이류에서는 아미노산 반응 시약으로 지문을 현출할 수 없어 oil red O (ORO)와 같은 지질 염색 시약 또는 physical developer (PD)를 적용해야 한다. 그러나 ORO는 4주 이상 된 오래된 지문에서 효과적이지 않다. 반면 PD는 오래된 지문을 잘 현출하지만 비교적 최근에 남겨진 지문에서는 효과적이지 아니하며, 지문 현출에 오랜 시간이 소요된다는 단점이 있다. 본 연구에서는 이러한 PD의 한계를 해결하기 위해 vacuum metal deposition (VMD)으로 은을 증착한 후 PD를 적용하여(Ag-PD) PD의 반응성을 높이고자 하였고, 이를 ORO, PD로 현출한 지문과 비교하였다. 그 결과, Ag-PD는 2~8주 동안 보관된 젖은 종이에서 ORO, PD보다우수한지문현출력을보였으며 PD의지문현출시간은 90~150 초로 대폭 감소하였다.

• 분석과학
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• 제17권3호
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• pp.271-277
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• 2004

The aim of this study was to investigate questioned documents written with blue or black ballpoint pen on paper by nondestructive technique. In this work, 21 blue and 22 black ballpoint pen inks which were purchased on different brands were analyzed by using Microspectrophotometry (MSP). The reflectance spectra were obtained from these ink samples and their shapes and the wavelength of the maximum intensity were compared. In the blue and black ballpoint pen inks, the discriminating powers (DP) were 0.85 and 0.61, respectively. The changes of the reflectance intensity at their wavelength of maximum intensity and their shapes appeared according to the exposure time to sunshine in a laboratory, especially in the blue ballpoint pen inks. Therefore it is possible to distinguish ink entries on the same paper with the relative age in case of questioned letters written with blue ballpoint pen.

• 분석과학
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• 제17권4호
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• pp.315-321
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• 2004

The aim of this study was to investigate the evidential value of blue and black ballpoint pens on paper by nondestructive techniques. In this work, 21 blue and 22 black ballpoint pens which were purchased on different brands were analyzed by Raman Spectroscopy and Video Spectral Comparator (VSC). Surface-Enhanced Resonance Raman Spectroscopy (SERRS) with excitation at 685 nm and VSC with several spot light filters were used for the discrimination of ballpoint pen inks. In the SERR spectra, the ballpoint pen inks on paper could be shown sharp spectral bands and distinguished by their band shapes and relative intensities. In the blue and black ballpoint pen inks, the discriminating powers (DP) by SERRS were 0.85 and 0.67 and the DP by VSC were 0.88 and 0.90, respectively. The DP by combined sequence of techniques was all 0.97 in both black and blue ballpoint pen inks.

• 분석과학
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• 제19권1호
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• pp.103-106
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• 2006

A 35-year-old Perubian who suffered from grand mal seizures died in the aircraft on his way from the United States to Hongkong via Incheon international airport of Korea. While performing the autopsy, 115 packs made with double layer of transparent film and black plastic bag containing cocaine were found in the ileum and large intestine. Among of them, 3 packs were ruptured. To determine the concentration of cocaine and its metabolites, blood, urine, bile, liver, spleen, heart, kidney, brain and gastric contents were taken and toxicological analysis was performed. Gas chromatography/mass spectrometry (GC/MS) following liquid-phase extraction using chloroform:isopropanol (=9:1) and derivatization with bis(trimethylsilyl)-trifluoroacetamide (contains 1% trimethylchlorosilane) was performed. High levels of cocaine, benzoylecgonine (BE) and ecgonine methylester (EME) were found in the postmortem blood (0.96, 3.09 and $5.59{\mu}g/mL$) and urine (32.85, 145.35 and $53.17{\mu}g/mL$), respectively. Cocaine and its metabolites were also detected in all other biological specimen.

• 대한의생명과학회지
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• 제8권4호
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• pp.257-268
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• 2002

Chronic infection and inflammation have recently been implicated as important etiologic agents of atherosclerosis. Several agents have been suggested as possible candidates including cytomegalovirus (CMV), herpes simplex vims type 1 (HSV-1), Epstein-Barr virus (EBV), Chlamydia pneumoniae, and Helicobacter pylori. We evaluated the relationship between cytornegalovirus infection and atherosclerosis by in situ hybridization and polymerase chain reaction (PCR). We examined 23 subjects with atherosclerosis and 10 matched control subjects without atherosclerosis. CMV was detected by in situ hybridization in 60.9% (14/23) of aorta and 42.9% (9/21) of coronary arteries in subjects with atherosclerosis. It was also detected by PCR in 65.2% (15/23) of aorta and 52.4% (11/21) of coronary arteries. CMV was detected on areas showing early or advanced atheromatous changes. Cells morphologically identical to smooth muscle cells, endothelial cells, lymphocytes, fibroblasts, and Schwann cells were positively reacted with the CMV probe. However. none of the cells to which the probe hybridized contained inclusion bodies, thus strongly suggesting that the arterial wall may be a site of CMV latency. This result Indicates that CMV may potentially play a direct or indirect role in the pathogenesis of human atherosclerosis.