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http://dx.doi.org/10.5806/AST.2022.35.6.242

Effect of ethylenediamine on luminol (or Bluestar) - bleach reaction  

Seulgi, Jang (Graduate School of Forensic Science, Soonchunhyang University)
Minkyoung, Kim (Graduate School of Forensic Science, Soonchunhyang University)
Heejin, Kim (Graduate School of Forensic Science, Soonchunhyang University)
Munhee, Lee (Graduate School of Forensic Science, Soonchunhyang University)
Sungwook, Hong (Graduate School of Forensic Science, Soonchunhyang University)
Publication Information
Analytical Science and Technology / v.35, no.6, 2022 , pp. 242-248 More about this Journal
Abstract
The effect of ethylenediamine (EDA) on the reaction of luminol or Bluestar with blood and bleaches was studied. For this purpose, blood, chlorine bleach, and oxygen bleach were applied to filter paper, treated with EDA-containing luminol or Bluestar, and the changes in chemiluminescence intensity were observed. It was found that the chemiluminescence intensity of the luminol (or Bluestar)-blood reaction did not change with the increasing concentration of EDA. However, the chemiluminescence intensity of the luminol (or Bluestar)-chlorine bleach reaction decreased and the chemiluminescence intensity of the luminol (or Bluestar)-oxygen bleach reaction increased, with increasing EDA concentration. Thus, it was found that when EDA was added to luminol (or Bluestar), which is a blood-sensitive reagent, EDA suppressed the false-positive reaction induced by chlorine bleach and induced a false-positive reaction with oxygen bleach. Consequently, the addition of EDA is not recommended for enhancing bloodstains with luminol or Bluestar.
Keywords
luminol; Bluestar; ethylenediamine; bleach; false-positive; false-negative;
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  • Reference
1 H. C. Lee, T. Palmbach, and M. T. Miller, 'Henry Lee's crime scene handbook', 1st Ed, Academic Press, London, 2001.      
2 T. G. Schotman, A. A. Westen, J. Weerd, and K. G. Bruin, Forensic Sci. Int., 257, 214-219 (2015).         DOI
3 S. J. Seashols, H. D. Cross, and D. L Shrader, J. Forensic Sci., 58(1), 130-133 (2013).         DOI
4 F. Barni, S. W. Lewis, A. Berti, G. M. Miskelly, and G. Lago, Talanta, 72(3), 896-913 (2007).         DOI
5 J. C. Niebauer, J. B. Booth, and B. L. Brewer, J. Forensic Identif., 40(5), 271-278 (1990).      
6 K. Virkler and I. K. Lednev, Forensic Sci. Int., 188(1-3), 1-17 (2009).      
7 J. L. Webb, J. I. Creamer, and T. I. Quickenden, Luminescence, 21(4), 214-220 (2006).         DOI
8 Bluestar Forensic, https://www.bluestar-forensic.com/, Assessed 4 April 2022.      
9 L. Dilbeck, J. Forensic Identif., 56(5), 706-720 (2006).      
10 K. J. Farrugia, K. A. Savage, H. Bandey, T. Ciuksza, and N. N. Daeid, Science & Justice, 51(3), 110-121 (2011).         DOI
11 L. T. Lytle and D. G. Hedgecock, J. Forensic Sci., 23(3), 550-562 (1978).      
12 T. I. Quickenden and P. D. Cooper, Luminescence, 16(3), 251-253 (2001).         DOI
13 J. I. Creamer, T. I. Quickenden, M. V. Apanah, K. A. Kerr, and P. Robertson, Luminescence, 18(4), 193-198 (2003).         DOI
14 E. Kent, D. Elliot, and G. Miskelly, J. Forensic Sci., 48(1), 1-4 (2003).      
15 M. Bancirova, Science & Justice, 52(2), 102-105 (2012).         DOI
16 A. Castello, M. Alvarez, and F. Verdu, J. Can. Soc. Forensic Sci., 35(3), 113-121 (2002).         DOI
17 A. Castello, F. Frances, D. Corella, and F. Verdu, Naturwissenschaften, 96(2), 303-307 (2009).         DOI
18 D. Howard, J. Chaseling, and K. Wright, Forensic Sci. Int., 302, 109885 (2019).      
19 M. Grodsky, K. Wright, and P. Kirk, J. Crim. L. Criminology & Police Sci., 42, 95 (1951).      
20 K. Weber and Z. Dtsch, Gesamte Gerichtl. Med., 57(3) 410-423 (1966).      
21 M. H. Lee, 'A study on the chemiluminescence intensity variation and the method of inhibiting the false-positive reaction of the blood diluted with chlorinated bleach based on drying time, (M.Sc. Thesis)', Soonchunhyang University, Asan, 2018.      
22 K. Hool and T. A. Nieman, Anal. Chem., 60, 834-837 (2010).    DOI
23 S. Cullen, A. Otto, and P. Cheetham, J. Forensic Identif., 60(1), 45-86 (2010).