• Korean Journal of Veterinary Research
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• v.60 no.1
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• pp.25-32
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• 2020

Natural killer (NK) cells play have a crucial role in the early phase of immune responses against various pathogens. We compared characteristics of canine NK cells against two canine mammary carcinoma cell lines, REM134 and CF41.Mg. REM134 showed higher expression of progesterone receptor, proliferative cell nuclear antigen, Ki67, multiple drug resistance, Bmi-1, c-myc, E-cadherin, and human epidermal growth factor receptor type-2 than that of CF41.Mg. For specific expansion and activation of NK cells, we isolated CD5 negative cells from canine peripheral blood mononuclear cells and co-cultured K562 cells in the presence of interleukin (IL)-2, IL-15, and IL-21 for 21 days. As a result, we found that expression markers of activated NK cells such as NKp30, NKp44, NKp46, NKG2D, CD244, perforin, granzyme B, and tumor necrosis factor alpha were highly upregulated. In addition, we found there was upregulated production of interferon gamma of activated NK cells against target cells such as REM134 and CF41.Mg. Specifically, we observed that cytotoxicity of NK cells against target cells was more sensitively reacted to CF41.Mg than REM134. Based on the results of this study, we recommend the development of an experimental application of CF41Mg, which has not been reported in canine mammary carcinoma research.

• The Journal of the Korean Society for Microbiology
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• v.13 no.1
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• pp.75-79
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• 1978

There has been many investigations on phytoagglutinin and especially, Moon et al reported a number of research works on phytoagglutinins prepared from the Korean plants. The present report describes results of experiment on the biological effect of 14 Korean phytoagglutinins to microorganism for the first time. 1) Kaolin rubi and Ricinus communis L. among 14 different species of Korean phytoagglutinins had agglutinating activities to microoganisms. 2) Kaolin rubi agglutinated E. coli, Staph. aureus, Ps. aeruginosa, Prot. vulgaris, B. subtilis, Sal. typhi, Sh. dysenteriae, C. albicans and Sa. cerevisiae but Ricinus communis L. showed only agglutination of Sa. cerevisiae. 3) Agglutinating titers of Kaolin rubi to various microorganisms were 500-1,000 but titer of Ricinus communis L. was only 50. 4) Ricinus communis L. showed bactericidal action to Sa. cerevisiae but Kaolin rubi had no such effect.

• Journal of Yeungnam Medical Science
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• v.38 no.3
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• pp.235-239
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• 2021

Accessory cavitated uterine mass (ACUM) is a rare and unique condition seen in young women. We report cases of ACUMs in two patients, a 14-year-old girl and a 25-year-old woman, both with complaints of severe dysmenorrhea that had started at menarche and had progressively worsened since. A large cystic lesion was localized in the anterolateral wall of the myometrium separate from the endometrium, which was difficult to distinguish from congenital uterine anomalies. Laparoscopic excision of the ACUMs was successful and completely resolved the dysmenorrhea. Early investigation of severe dysmenorrhea in young women can provide appropriate management and relieve symptoms.

• Biomedical Science Letters
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• v.16 no.3
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• pp.197-200
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• 2010

All citizens over 17 year old living in Korea have to be fingerprinted to obtain a certificate of resident registration. For this reason, human identification through fingerprints has been used actively in crime scene investigation. The fingerprint is so unique that it is one of the most certain ways to identify oneself and it can differentiate between genetically identical twins. Fingerprints gained in crime scene indicate a direction of criminal investigation in conjecturing a suspect. Fingerprints help a reunion of family got scattered for a long time and make it possible to get a personal identification for missing person who met with natural calamity. We developed a new fingerprinting method using safranine O, so as to develop fingerprints on the adhesive tapes and non-porous papers in various physical environments. Results were compared to the preexisting fingerprinting method, the minutiae numbers of fingerprints were greatly increased in our newly developed safranine O fingerprinting method. This newly developed safranine O method showed a quantity and quality comparable to the preexisting fingerprinting method routinely used in these days. In our hands, the safranine O fingerprinting method is another easy and obvious choice when the forensic case sample is available for fingerprints on the adhesive tapes and non-porous papers.

• Anatomy and Cell Biology
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• v.55 no.4
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• pp.507-511
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• 2022

A three-dimensional (3D) segmentation and model reconstruction is a specialized tool to reveal spatial interrelationship between multiple internal organs by generating images without overlapping structures. This technique can also be applicable to mummy studies, but related reports have so far been very rare. In this study, we applied 3D segmentation and model reconstruction to computed tomography images of a Korean mummy with congenital diaphragmatic hernia. As originally revealed by the autopsy in 2013, the current 3D reconstruction reveals that the mummy's heart is shifted to the left due to the liver pushing up to thoracic cavity thorough diaphragmatic hernial defect. We can generate 3D images by calling up the data exclusively from mummy's target organs, thus minimizing the confusion of diagnosis that could be caused by overlapping organs.

• Journal of Oral Medicine and Pain
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• v.38 no.3
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• pp.235-246
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• 2013

Age estimations by forensic odontology play a great role in personal identification. The purpose of this study was to analyze the characteristics of age estimation cases in Korea. Surveying clients who requested age estimation at the Department of Oral Medicine, Kyungpook National University Dental Hospital from March 2012 to March 2013. The results were as follows: 1. On gender distribution, females(57.14%) were more than males(42.86%). The elderly with 50's and over 60's were majorities(89.28%) of clients, and no clients were below 40's. Most of clients were equal to and lower than elementary school graduate(69.64%). 2. The most frequent reason for age discrepancy between registered age and alleged age was mistakes by family or relatives(80.36%). The purposes of age estimation were welfare(62.50%), social relationship problem(12.50%), to find right age(10.71%), and occupation(8.93%). 3. In order of route to visit at Department of Oral Medicine, they were via government office(48.21%), acquaintances(21.43%), mass media(14.29%), and clinic(10.71%). Clients had high degree of comprehension on age estimation with forensic odontology (scored 7.03 out of 10). The 2/3 of clients were satisfied with present fee for age estimation. 4. The percentage on the proximity of estimated age to alleged age was 69.81%. 4(11.43%) clients were approved on age correction by court.

• Journal of Preventive Medicine and Public Health
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• v.49 no.2
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• pp.134-138
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• 2016

Objectives: Season of birth, an exogenous indicator of early life environment, has been related to higher risk of adverse psychiatric outcomes but the findings for Alzheimer's disease (AD) have been inconsistent. We investigated whether the month or season of birth are associated with AD. Methods: A nationwide nested case-control study including all community-dwellers with clinically verified AD diagnosed in 2005 to 2012 (n=70 719) and up to four age- sex- and region of residence-matched controls (n=282 862) residing in Finland. Associations between month and season of birth and AD were studied with conditional logistic regression. Results: Month of birth was not associated with AD (p=0.09). No strong associations were observed with season (p=0.13), although in comparison to winter births (December-February) summer births (June-August) were associated with higher odds of AD (odds ratio, 1.03; 95% confidence interval, 1.00 to 1.05). However, the absolute difference in prevalence in winter births was only 0.5% (prevalence of those born in winter were 31.7% and 32.2% for cases and controls, respectively). Conclusions: Although our findings do not support the hypothesis that season of birth is related to AD/dementia risk, they do not invalidate the developmental origins of health and disease hypothesis in late-life cognition. It is possible that season does not adequately capture the early life circumstances, or that other (postnatal) risk factors such as lifestyle or socioeconomic factors overrule the impact of prenatal and perinatal factors.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.22
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• pp.9693-9698
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• 2014

Background: Associations between the 8473T>C polymorphism (rs5275) in the cyclooxygenase-2 (COX-2) gene and breast cancer (BC) risk are still inconclusive and ambiguous. The aim of this meta-analysis was to comprehensively estimate the genetic risk of 8473T>C polymorphism in the COX-2 gene for BC. Materials and Methods: We searched PubMed, Web of Science, Medline, Chinese biomedical (CBM), Weipu, China national knowledge infrastructure (CNKI), and Wanfang databases, covering all publications (last search was updated on Aug 17, 2014). Statistical analyses were performed using Revman 5.3 and STATA 10.0 software. Results: A total of 6,720 cases and 9,794 controls in 12 studies were included in this study. The results indicated no significant associations between the 8473T>C polymorphism of the COX-2 gene and BC risk for the CC+TC vs TT model (pooled odds ratio (OR)=0.97, 95% confidence interval (CI)=0.90-1.03, and p=0.29). On subgroup analysis, we also found that subdivision on ethnicity among Caucasians, Asians and others also revealed no relationship with BC susceptibility. With the study design (CC+TC vs TT), no significant associations were found in either population-based case-control studies (PCC), or hospital-based case-control studies (HCC). Conclusions: This present meta-analysis suggests that the 8473T>C polymorphism in the COX-2 gene is not a conspicuous low-penetrant risk factor for developing BC.

• Journal of Oral Medicine and Pain
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• v.23 no.2
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• pp.193-209
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• 1998

In identifying bodies that are severely decayed or damaged, methods using fingerprints and various biochemical tests are known to have its limits. To overcome this, forensic odontological method which is based on the analysis of the cranium, tooth and dental restoration is used to enhance the accuracey of individual identification. For this reason, I have come to analysis of the dental materials that exists between the teeth that is perceived to have been previously restored and the one adjacent to it. By analyzing the constituents of gold crown-restored, non-precious metal-restored, gold inlay-restored and amalgam -restored teeth, and adjacent teeth using EDX(energy dispersive X-ray microanalysis) which was invented to analyze very small amount of elements, the nature of the restoration could be predicted and the results obtained were as follows. 1. Some of constitute of gold alloy was extracted from residual cement of gold crown restoration, but that was not extracted from the restored tooth and the one adjacent to it. 2. Some of constituents of non-precious metal alloy was extracted both in the residual cement on the tooth with no-precious metal restoration and in the tooth with the restoration itself. However, none of its constituents were found in the tooth adjacent to it. 3. Some of constituents of gold alloy were found in the residual cement of gold inlay, but they were not found in the restored tooth and the adjacent tooth. 4. Some of constituents of amalgam alloy were found both in tooth restored with amalgam and in the adjacent tooth. From the results obtained above, it is possible to utilize the data obtained from analyizing residual dental materials in a more effective way. This data compensates for the lost data due to any harm done to the restorations prior to individual identification and further enhances the accuracy. Therefore, it could be concluded that this process of analyzing residual dental materials could be beneficial to individual identification in the area of forensic odontoldogy.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.3
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• pp.1141-1148
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• 2016

Background: A large number of studies have been published to investigate the association between the null genotype of glutathione S-transferase T1 (GSTT1) with gastric cancer. However, the results were inconsistent and conflicting. The aim of this study was to estimate the relationship between this polymorphism in the GSTT1 gene and gastric cancer risk in Asian populations by meta-analysis. Materials and Methods: A literature search was performed in PubMed, Embase, Chinese Biomedical database (CBM), Weipu database, Wanfang database, and China National Knowledge Infrastructure database (CNKI). Statistical analysis was conducted by using Review Manager 5.3. Results: Thirty-nine studies with a total of 7,737 gastric cancer cases and 10,823 controls were included in this meta-analysis. The meta-analysis of total studies showed that the null genotype in GSTT1 was associated with increased risk of gastric cancer in Asians (OR=1.19, 95% CI=1.08-1.31, p=0.0002). Subgroup analysis showed a significant relationship between GSTT1 null genotype and gastric cancer in East-Asians, as well as in subgroup analysis of hospital-based design. On subgroup analysis by smoking status, alcohol status, Helicobacter pylori infection status, and histology type, no significant association of this polymorphism with susceptibility to gastric cancer was found. Conclusions: In conclusion, the results showed that the null genotype of GSTT1 is significantly associated with an increased risk in gastric cancer in Asian populations.