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http://dx.doi.org/10.14405/kjvr.2020.60.1.25

Cytotoxicity of natural killer cells on canine mammary carcinoma cells  

Jeong, Da-Un (Viral Disease Research Division, Animal and Plant Quarantine Agency)
Byeon, Jeong Su (Viral Disease Research Division, Animal and Plant Quarantine Agency)
Gu, Na-Yeon (Viral Disease Research Division, Animal and Plant Quarantine Agency)
Jung, Moonhee (Department of Forensic Medicine, Chonnam National University Medical School)
Kim, Eun Hee (Department of Forensic Medicine, Chonnam National University Medical School)
Kim, Hyung-Seok (Department of Forensic Medicine, Chonnam National University Medical School)
Cho, In-Soo (Viral Disease Research Division, Animal and Plant Quarantine Agency)
Song, Jae-Young (Viral Disease Research Division, Animal and Plant Quarantine Agency)
Hyun, Bang-Hun (Viral Disease Research Division, Animal and Plant Quarantine Agency)
Lee, Jienny (Viral Disease Research Division, Animal and Plant Quarantine Agency)
Publication Information
Korean Journal of Veterinary Research / v.60, no.1, 2020 , pp. 25-32 More about this Journal
Abstract
Natural killer (NK) cells play have a crucial role in the early phase of immune responses against various pathogens. We compared characteristics of canine NK cells against two canine mammary carcinoma cell lines, REM134 and CF41.Mg. REM134 showed higher expression of progesterone receptor, proliferative cell nuclear antigen, Ki67, multiple drug resistance, Bmi-1, c-myc, E-cadherin, and human epidermal growth factor receptor type-2 than that of CF41.Mg. For specific expansion and activation of NK cells, we isolated CD5 negative cells from canine peripheral blood mononuclear cells and co-cultured K562 cells in the presence of interleukin (IL)-2, IL-15, and IL-21 for 21 days. As a result, we found that expression markers of activated NK cells such as NKp30, NKp44, NKp46, NKG2D, CD244, perforin, granzyme B, and tumor necrosis factor alpha were highly upregulated. In addition, we found there was upregulated production of interferon gamma of activated NK cells against target cells such as REM134 and CF41.Mg. Specifically, we observed that cytotoxicity of NK cells against target cells was more sensitively reacted to CF41.Mg than REM134. Based on the results of this study, we recommend the development of an experimental application of CF41Mg, which has not been reported in canine mammary carcinoma research.
Keywords
canine; mammary carcinoma; natural killer cells; interferon gamma; cytotoxicity;
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