• Korean Journal of Fisheries and Aquatic Sciences
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• v.49 no.1
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• pp.38-44
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• 2016

The quality and quantity of live food sources strongly influence the success of fish production in farming operations. Thus, critical studies of live forage species are a crucial element for progress in fish aquaculture. The fat content of food is an especially important determinant of growth in marine fish. Omega-3 highly unsaturated fatty acids (HUFA) are essential components of diet that determine the nutritional value of larval fish. Euglena is a protist that has potential as a forage species. These single-celled organisms have plant and animal characteristics they are motile, elliptical in shape and 15–500 μm in diameter. Their nutritional content is excellent, but most studies have focused on cells raised in autotrophic culture. We therefore examined differences in the lipid and fatty acid contents, and the growth of Euglena cells grown under autotrophic, heterotrophic, and mixotrophic conditions. Biomass production reached 15.03 g/L, 12.28 g/L, and 3.66 g/L under mixotrophy, heterotrophy, and autotrophy, respectively. The proportional n-3 HUFA content differed among culture methods: 10.04%, 5.80% and 10.01% in mixotrophic, heterotrophic and autotrophic cultures, respectively. Mixotrophy was to be the best form of cultivation for improving the growth and nutritional content of Euglena.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.12
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• pp.1587-1593
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• 2010

Pastures of the Apennines of Central Italy contribute to feed resources of high altitude (above 1,300 m sea level) grazing systems. The objective of this study was to evaluate the effectiveness of faecal extract from the yak (Bos grunnienes) as an alternative microbial inoculum to rumen fluid for estimation of digestibility of several forage species. Forage samples produced at high altitude were tested in this study: four legumes (Lathyrus sativus L., Lotus corniculatus L., Onobrychis viciaefolia L. and Trifolium pratense L.), three forbs (Achillea millefolium L., Potentilla reptans L. and Teucrium flavum L.) and one grass (Brachipodyum pinnatum L.) were incubated with yak rumen fluid or faecal extract. A large variability in chemical composition was observed among the species collected. Rumen liquor and faecal samples were collected from adult healthy yak. The $Daisy^{II}$ incubator was used to evaluate the nutrient digestibility of forages using rumen liquor as control and faecal extract as alternative microbial inoculum sources. Filter bags containing samples of browse species were added to the four digestion vessels along with their respective inoculum and then incubated for 48 h and dry matter (DM), organic matter (OM), crude protein (CP), neutral and detergent fiber (NDF) digestibility was determined. There was a significant relationship between estimates, indicating that faecal liquor has the potential to be used instead of rumen fluid for estimation of in vitro digestibility of plants. It is concluded that the $Daisy^{II}$ incubator results are appropriate for the determination of in vitro digestibility of nutrients using faecal liquor to define the potential for adaptation of yak to new pastures.

• Asian-Australasian Journal of Animal Sciences
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• v.6 no.1
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• pp.53-59
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• 1993

In situ degradability and fermentation characteristics in the rumen of goats fed whole crop corn forage ensiled with (MS silage) or without (CS silage) 30% of cage layer manure (CLM) were investigated. The two silages were well preserved. To adjust nitrogen intake of CS silage to that of MS silage, the 3rd group of goats was given urea with CS silage at feeding time (US silage). Each goat was given a diet of 2% of the body weight (dry matter basis) daily. In situ degradability of dry matter (DM) and crude protein (CP) of MS silage in the rumen were higher than those of CS and US silages. Total potentially degradable portions of DM and CP in MS silage were also higher than those in CS and US silages. Blood urea nitrogen and rumen ammonia nitrogen concentration of goats fed US and MS silages were significantly (p<0.05) higher than those of goats fed CS silage. Acetic, propionic and butyric acids in ruminal fluids of goats fed MS silage were significantly (p<0.05) higher than those of goats fed CS and US silages.

• Journal of The Korean Society of Grassland and Forage Science
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• v.11 no.1
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• pp.1-5
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• 1991

Tall fescue(Festuca amndinacea Schreb.) is a productive pasture grass that is widely adapted to the soil and climate of Korea. It is regarded, however, as relatively low in palatability and nutritive value. On the other hand, Italian ryegrass(lo1ium rnultz$om Lam.) is highly nutritious and palatable, but is essentially weak in cold tolerance. Numerous investigators have succeeded in producing hybrids between Lolium spp. and Festuca spp. but no improved varieties have resulted. Therefore, intergeneric hybridization of Italian ryegrass with tall fescue is employed in 1984 to improve the adaptive and cold resistance quality of Italian ryegrass. In the mean time, F1-hybrid plants obtained by crossing Italian ryegrass x tall fescue were observed in open field. The results are summarized as follows: 1. Leaf-size and silica cell hair were intermediate between the types of parents but the shape of auricle tended to show Italian ryegrass and auricle-cilia to show tall fescue. 2. Roots of $F_1$-hybrid plants were mainly distributed at top soil and did not produced florescence material. 3. Head type of hybrids was intermediate between the types of parents but ray length was somewhat less than the length of tall fescue ray. Typical Italian ryegrass or tall fescue-like phenotypes were not observed. 4. Anthers of $F_1$-hybrid plants were formed but pollen were not formed. Even though pollen were formed, they were all empty. 5. Hybrid plants were more winterhardy and summer persistency than Italian ryegrass. They showed a equivalent growth to Italian ryegrass in spring and to tall fescue in summer. 6. Chromosome of $F_1$-hybrids was allopolyploid(2n=4~=28) and all $F_1$-hybrid plants were sterile.

• Journal of The Korean Society of Grassland and Forage Science
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• v.21 no.4
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• pp.259-264
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• 2001

To investigate the physiological responses to naturally occurring winter freezing stress in creeping bentgrass, changes in carbohydrates were monitored during winter period. Turf quality and leaf growth was nearly parallel with temperature fluctuation. The concentration of glucose, fructose and sucrose in both shoot and root gradually increased from November to January, and then sharply decreased until April. Sucrose was the largest pool of soluble sugars. Fructan also slightly accumulated in both shoot and roots from November to February. Fructan hydrolysis in both organs was found to be much active between February to April. Shoot contained largely higher carbohydrate content in all compounds examined than roots did. Fructan was found to be a main carbohydrate storage form, showing the highest concentration (176.7 and 126.7 mg g-' DW for shoot and root in February). The depolymerization of fructan from February coincided with the high declines in mono- and disaccharide. These results suggest that the accumulation of non-structural carbohydrate until January could be associated with freezing tolerance, and the active decrease from February with shoot regrowth.

• Journal of The Korean Society of Grassland and Forage Science
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• v.4 no.2
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• pp.152-157
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• 1983

This study was carried out to investigate changes in the quality and botanical composition of native pasture during the grazing period. The results obtained were as follows: 1. On all the farms, the total dry matter yield of native pasture was much higher in the October-cut than in the July-cut. 2. The crude protein content was higher in the July-cut than in the Oct.-cut, while the crude fiber content gave opposite results. There was the same tendency on all the farms. 3. When the six farms were compared, the highest crude protein content was found at Hawon and Haian farm, and cows on these farms showed the highest daily weight gain. Ohra farm had the lowest crude protein and the cows on this farm the lowest daily weight gain. 4. The botanical composition of the main species distributed was 58.1% Zoysia japonica, 16.4 Pteridum aquilinum, and 3.8% Miscanthus sinensis. In the case of family, 72.1% were Gramineae, 1.8% Leguminosae, and 16.4% Pteridacea. 5. According to botanical composition of a family, Hawon farm had the highest daily weight gain and the highest rate of Gramineae and Leguminosae. Ohra and Youngkang farms had the lowest weight gains, but the native grassland on these farms contained a high proportion of Pteridum which is toxic to cattle.

• Journal of The Korean Society of Grassland and Forage Science
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• v.42 no.4
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• pp.270-275
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• 2022

Livestock production costs are heavily influenced by the cost of feed, The use of domestically grown forages is more desirable for livestock feed production. As part of this study, triticale, which is an extremely palatable and easily cultivable crop in Korea, was used to produce low moisture silage bales with lactic acid bacteria (LAB) and then stored for different periods. We examined the nutrient content of silage, such as crude protein (CP), acid detergent fiber (ADF) and neutral detergent fiber (NDF), as well as their organic acids, including lactic acid, acetic acid, butyric acid, at different storage periods. The nutrient content of silages, such as crude protein, ADF, and NDF, did not change significantly throughout storage periods. Organic acid data indicated that lactic acid concentrations increased with increasing moisture contents and storage periods up to nine months. However, further extending storage to 12 months resulted in a reduction in the lactic acid content of all silages as well as an increase in their pH. Based on the present results, it suggested that the production of low moisture silage with the LAB may be able to preserve and maintain its quality without altering its nutritional composition. Also, the lactate content of the silage remained significant for at least nine months.

• Asian-Australasian Journal of Animal Sciences
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• v.11 no.3
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• pp.272-276
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• 1998

Sheep and goats in Pakistan have not been able to produce to the best of their potential. This may primarily be attributed to under feeding and malnutrition. Ranges have been depleted due to overgrazing and mismanagement and are not in position to feed the existing small ruminant population. To overcome the shortage of good quality fodder and balanced feed supply, the alternate means like cereal straws and other crop residues are being commonly used. Sunflower crop residues like stalks and heads provide a good quality forage for livestock. These crop byproducts are rich in crude protein and lower in crude fibre. Their inclusion in the diet of small ruminants at 20, 30 and 40 percent levels in ration has shown significantly (p < 0.05) increased feed intake and weight gain. Daily feed intake was 1,130, 1,180 and 1,750 g for sunflower crop residue, soybean crop residue and wheat straw, respectively, when added at the rate of 20 percent in the ration. The drymatter digestibility of sunflower, soybean crop residues and wheat straw was also comparable. The maximum performance of the animals was observed at the 20 percent level of inclusion of sunflower crop residue in the diet.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.8
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• pp.1133-1137
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• 2001

An experiment was conducted to study the performance and carcass characteristics of Hanwoo steers fed whole crop corn silage ensiled with poultry manure (PM) for 18 months. The experiment was designed as a randomized block design with three phases. Steers were allotted in one of three dietary treatments, which were ammonia-treated rice straw (AS), whole crop corn silage (CS) and whole crop corn+30% PM silage (based on DM; MS). All diets were supplemented with concentrate. Total body weight gain and average daily gain (ADG) in MS group were increased (p<0.05) by 6% over AS group. The MS treatment enhanced (p<0.05) total and daily intakes of forage compared with the AS and CS treatments while there was compensatory effect on concentrate intake by AS group. Carcass characteristics were improved by feeding MS. MS increased (p<0.05) carcass weight and marbling score (7.5 and 22.5% of AS, respectively), and reduced (p<0.05) backfat thickness (13.2 of AS and 16.6% CS). Carcass grade and meat quality grade were also improved by MS compared with AS and CS. Under the conditions of this study, MS was an efficacious replacement for corn silage for steers.

• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.1
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• pp.41-48
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• 1999

This experiment was carried out to determine the effect of the silage additives on improvement of quality of fresh rice straw silage using round bale at the forage experimental field, grassland and forage crops division, National Livestock Research Institute, RDA, Suwon from 1997 to 1998. The experiment was arranged in a randomized block design with three replication. The treatments used in this study were consisted of different additives(control, formic acid, molasses, molasses+urea and inoculant). The rice straw silage with molasses+urea treatment resulted in high crude protein content and in vitro dry matter digestibility were increased with molasses of inoculant treatments compare with the control. The mean dry matter of formic acid treatment material was higher than with control but there was no significant difference in dry matter content among the additives treatments. The pH of molasses treatments significantly increased the proportion of lactic acid(P<0.05) and decreased the proportion of butyric acid. The total organic acid content of all treatments had low around 2%. Ammonia-N of molasses+urea treatment was significantly(P<0.05) higher than that of others, but formic acid or inoculant treatments was lower below 10% per total nitrogen. Over a 7d feeding period, the dry matter intake per cattle on the inoculant treatment was higher that on both the untreated round bale silage of fresh rice straw and rice straw hay. Producing cost per kilogram of round bale silage of fresh rice straw was decreased according to the increasement of harvesting area. It is suggested that application of round bale silage system to fresh rice straw with molasses or inoculant was the best treatment for improving preservation as silage, and that animal intake was enhanced by addition of inoculant to fresh rice straw.