• Biomolecules & Therapeutics
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• 제27권4호
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• pp.373-380
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• 2019

Sphingosine kinase 1 and its product, sphingosine 1-phosphate (S1P), as well as their receptors, have been implicated in inflammatory responses. The functions of receptors $S1P_1$ and $S1P_2$ on cell motility have been investigated. However, the function of $S1P_3$ has been poorly investigated. In this study, the roles of $S1P_3$ on inflammatory response were investigated in primary peritoneal macrophages. $S1P_3$ receptor was induced along with sphingosine kinase 1 by stimulation of lipopolysaccharide (LPS). LPS treatment induced inflammatory genes, such iNOS, COX-2, $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$. TY52156, an antagonist of $S1P_3$ suppressed the induction of inflammatory genes in a concentration dependent manner. Suppression of iNOS and COX-2 induction was further confirmed by western blotting and NO measurement. Suppression of $IL-1{\beta}$ induction was also confirmed by western blotting and ELISA. Caspase 1, which is responsible for $IL-1{\beta}$ production, was similarly induced by LPS and suppressed by TY52156. Therefore, we have shown $S1P_3$ induction in the inflammatory conditions and its pro-inflammatory roles. Targeting $S1P_3$ might be a strategy for regulating inflammatory diseases.

• 한국가정과교육학회지
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• 제29권4호
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• pp.133-147
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• 2017

본 연구는 광주지역에 거주하는 중학생을 대상으로 김치에 대한 인식과 섭취실태에 대해 조사하고 김치 인식도에 따른 김치 섭취의 차이를 분석하여, 학생 스스로 김치에 대해 재인식 할 수 있도록 하고, 학생들의 올바른 식습관 형성에 도움을 주는 기초 자료를 제공하고자 하였다. 광주지역에 거주하는 중학생을 대상으로 구조화된 설문지로 자료를 수집하였고, 374부를 최종 분석에 사용하였다. 중학생의 김치 인식도는 1학년은 4.10점, 3학년은 3.92점으로 1학년이 3학년보다 김치에 대한 인식이 높았다(p<0.001). 중학생의 김치 기호도는 '김치를 좋아하는 정도'(p<0.001), '김치를 먹지 않는 이유'(p<0.05)에서 1학년과 3학년이 유의한 차이가 나타났다. '김치를 좋아하는 정도'에서 김치를 좋아하는 학생은 1학년이 81.7%, 3학년이 59.8%로 1학년이 김치를 더 좋아함을 알 수 있었다(p<0.001). 중학생의 김치 섭취 실태는 '김치 섭취 횟수'(p<0.05), '한번에 섭취하는 김치 양'(p<0.05) 항목에서 1학년과 3학년이 유의한 차이가 나타났다. 김치 섭취 횟수는 1학년은 하루에 한번 집에서 섭취하는 비율이 높았고, 3학년은 하루 3회 이상 섭취하는 비율이 높았다(p<0.05). 중학생의 김치 교육은 '김치 권유에 대한 나의 태도'(p<0.05), '김치 섭취 교육의 필요성'(p<0.05), '김치 정보를 듣고 좋은 음식이라고 생각한 적의 여부'(p<0.001), '희망하는 김치 교육 방법'(p<0.001), '김치 섭취를 높이기 위한 방법'(p<0.05)에서 1학년과 3학년이 유의한 차이가 나타났다. 중학생의 김치 인식도에 따른 김치 기호도는 '김치를 좋아하는 정도'(p<0.001), '김치를 좋아하는 이유'(p<0.05), '김치를 싫어하는 이유'(p<0.05)에서 김치 인식도에 따라 유의한 차이가 나타났다. 즉, 김치 인식도가 높을수록 기호도가 높은 경향을 보였고, 김치 특유의 맛을 좋아하였다. 김치 인식도에 따른 김치 섭취 실태는 '김치 섭취 횟수'(p<0.001), '한번에 섭취하는 김치 양'(p<0.05)에서 김치 인식도에 따라 유의한 차이가 나타났다. 즉, 김치 인식도가 높을수록 김치 섭취 횟수와 섭취량도 더 많은 경향을 보였다. 김치 인식도에 따른 김치 교육은 '김치 권유에 대한 나의 태도'(p<0.05), '김치를 담근 경험'(p<0.05), '김치 섭취 교육의 필요성'(p<0.001), '김치 정보를 듣고 좋은 음식이라고 생각한 적의 여부'(p<0.001)에서 김치 인식도에 따라 유의한 차이가 나타났다. 즉, 김치 인식도가 높을수록 김치를 권했을 때의 태도 변화가 긍정적이었고, 김치를 담근 경험이 있는 학생일수록 인식도 높았으며 김치 섭취의 교육도 필요하다고 하였다.

• Journal of Microbiology and Biotechnology
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• 제7권6호
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• pp.391-396
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• 1997

Two series of carbon sources, linear primary $C_1$~$C_9$ alcohols and linear $C_2$~$C_{10}$ monocarboxylic acids were tested for PHA synthesis in Paracoccus denitrificans. The results showed that the growth-associated synthesis of PHA could be referred only to the carbon sources with odd number of carbon except methanol. For all carbon sources with even number of carbon, nitrogen limitation was required to induce PHA synthesis in P. denitrificans. Poly(3-hydroxyvalerate)[P(3HV)] homopolymer was synthesized from $C_5$, $C_7$, and $C_9$ while growing in the presence of nitrogen, but the nitrogen depletion in the later growth period incorporated 3-hydroxybutyrate(3HB) unit into the polymer chain. The optimum C/N ratio for P(3HV) homopolymer production was found to be 10 when the strain was grown on 10 ml/l of valeric acid for 96 h. P. denitrificans synthesized P(3HB-co-3HV) copolymers from n-hexanoic and n-octanoic acid. The microstructural characterics of the P(3HB-co-3HV) copolymer from n-propanol was investigated using $^13C$-nuclear magnetic resonance spectroscopy, showing a structural heterogeneity.

• Molecules and Cells
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• 제45권10호
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• pp.718-728
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• 2022

Splicing factor B subunit 4 (SF3B4), a component of the U2-pre-mRNA spliceosomal complex, contributes to tumorigenesis in several types of tumors. However, the oncogenic potential of SF3B4 in lung cancer has not yet been determined. The in vivo expression profiles of SF3B4 in non-small cell lung cancer (NSCLC) from publicly available data revealed a significant increase in SF3B4 expression in tumor tissues compared to that in normal tissues. The impact of SF3B4 deletion on the growth of NSCLC cells was determined using a siRNA strategy in A549 lung adenocarcinoma cells. SF3B4 silencing resulted in marked retardation of the A549 cell proliferation, accompanied by the accumulation of cells at the G0/G1 phase and increased expression of p27, p21, and p53. Double knockdown of SF3B4 and p53 resulted in the restoration of p21 expression and partial recovery of cell proliferation, indicating that the p53/p21 axis is involved, at least in part, in the SF3B4-mediated regulation of A549 cell proliferation. We also provided ubiquitination factor E4B (UBE4B) is essential for p53 accumulation after SF3B4 depletion based on followings. First, co-immunoprecipitation showed that SF3B4 interacts with UBE4B. Furthermore, UBE4B levels were decreased by SF3B4 depletion. UBE4B depletion, in turn, reproduced the outcome of SF3B4 depletion, including reduction of polyubiquitinated p53 levels, subsequent induction of p53/p21 and p27, and proliferation retardation. Collectively, our findings indicate the important role of SF3B4 in the regulation of A549 cell proliferation through the UBE4B/p53/p21 axis and p27, implicating the therapeutic strategies for NSCLC targeting SF3B4 and UBE4B.

• BMB Reports
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• 제55권2호
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• pp.104-109
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• 2022

Skeletal myogenesis is essential to keep muscle mass and integrity, and impaired myogenesis is closely related to the etiology of muscle wasting. Recently, miR-141-3p has been shown to be induced under various conditions associated with muscle wasting, such as aging, oxidative stress, and mitochondrial dysfunction. However, the functional significance and mechanism of miR-141-3p in myogenic differentiation have not been explored to date. In this study, we investigated the roles of miR-141-3p on CFL2 expression, proliferation, and myogenic differentiation in C2C12 myoblasts. MiR-141-3p appeared to target the 3'UTR of CFL2 directly and suppressed the expression of CFL2, an essential factor for actin filament (F-actin) dynamics. Transfection of miR-141-3p mimic in myoblasts increased F-actin formation and augmented nuclear Yes-associated protein (YAP), a key component of mechanotransduction. Furthermore, miR-141-3p mimic increased myoblast proliferation and promoted cell cycle progression throughout the S and G2/M phases. Consequently, miR-141-3p mimic led to significant suppressions of myogenic factors expression, such as MyoD, MyoG, and MyHC, and hindered the myogenic differentiation of myoblasts. Thus, this study reveals the crucial role of miR-141-3p in myogenic differentiation via CFL2-YAP-mediated mechanotransduction and provides implications of miRNA-mediated myogenic regulation in skeletal muscle homeostasis.

• 융합정보논문지
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• 제9권3호
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• pp.104-111
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• 2019

본 연구의 목적은 대체의학기술인 SUKI(Superficial Using Ki energy Instrument) 점압법을 활용한 특정근육(상부승모근 trigger point) 부분을 자극하여 스트레스를 받은 뇌파의 변화를 알아보고자 실시하였다. 연구방법은 스트레스에 의해 승모근통증을 호소하는 12명의 남녀 대학생을 대상으로 실시하였다. 상부승모근 통증유발점에 SUKI를 사용하기 전후의 특정영역의 뇌파를 측정하였다. SUKI자극시간은 3min 자극한 후 각 부위별로 비교하였으며(FP2, F3, F4, FP1, T3, T4, P3, P4), 실험기간은 총 5회 실시하였다. QEEG-S 뇌파측정기를 이용하였고, 데이터 수집을 위해 Telescan(LXE 5208) 프로그램을 사용하였다. 자료분석은 SPSS 22.0 version을 사용하였고, 점압법 전후 차이를 비교하기 위하여 대응표본 t-검정(paired t-test)을 실시하였으며, 통계학적인 유의수준은 p<.05로 설정하였다. 연구결과는 다음과 같다. FP2, F3, F4(*p<0.049, *p<0.042, *p<0.019)는 뇌파는 유의하게 나타났고, FP1, T3, T4, P3, P4는 유의하지 않았다. 따라서 SUKI 대체의학기술에 의한 뇌스트레스 감소 효과가 있었다. 추후 SUKI 대체 의학의 지속적인 연구가 요청된다.

• 한국구조물진단유지관리공학회 논문집
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• 제26권1호
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• pp.89-96
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• 2022

이 논문에서는 건설용 ME 방식 3D 프린터로 출력하기 위한 시멘트 복합체 배합의 출력품질을 평가하기 위해 출력 외관의 육안검사와 출력물의 치수 오차율, 압축강도 및 휨강도를 측정하였다. 시험결과, 출력 불연속 정도, 표면 무결성, 형태 유지능력, 출력가능 여부와 같은 4개 지표를 모두 만족하는 우수한 배합은 P1-2, P1-4, P2-5, P2-6으로 나타났다. 3개 지표를 만족하는 배합은 P0-1, P1-1, P1-3, P1-6, P1-7, P2-4으로 나타났다. 이 배합 중 오차율 측정용 중공 원주형 공시체의 직경 치수 오차율과 높이 치수율이 낮은 배합은 P0-1, P2-6로 나타났다. 치수 오차율이 우수한 배합과 그렇지 않은 배합의 압축강도 및 휨강도를 평가한 결과 출력 품질이 우수한 배합의 역학적 특성이 우수한 것으로 나타났다. 그러나, 역학적 특성이 우수한 배합이 반드시 출력 품질이 우수한 것은 아니기 때문에 정확한 품질평가를 위해서는 출력 외관에 대한 육안검사와 치수 오차율 검사를 선행하여 역학적 특성을 검토해야 할 것으로 판단된다.

• 한국세라믹학회지
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• 제25권2호
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• pp.136-142
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• 1988

β-Al2O3, which is used for solid electrolyte membrances in sodium-sulfur batteries, was prepared by sol-gel process. Sodium-n-propoxide NaOC3H7 and aluminum-isopropoxide Al(OC3H7)3 were hydrolyzated in the solution at pH 3, pH 7, pH 9 and pH 11, respectively. The sol-gel processed samples were calcined at several temperature steps, respectively and analysed by thermal analyser(DT-TGA), infrared spectrum analyser and X-ray diffraction analyser. The gelling rate of solution at pH 7 was much higher than that of the solution at pH 3. Thermal exchanging behavior of the gels at pH 3 were similar to Na2O·Al2O3·6H2O and, above pH 7, were similar to Na2O·Al2O3·3H2O. When samples' composition ratio was 9.13 : 90.87 [NaOC3H7:Al(OC3H7)3] at pH 7, β-Al2O3 was formed at 1100℃.

• 구강회복응용과학지
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• 제32권1호
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• pp.16-23
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• 2016

목적: 이번 연구는 다양한 pH의 불소 제재들이 티타늄 표면 거칠기에 미치는 영향을 평가하는 것이었다. 연구 재료 및 방법: 기계절삭형 티타늄 디스크를 시중에서 유통되는 세 가지 불소겔로 처리하였다. 불소겔의 종류와 처리 시간에 따라, 대조군, 1군(pH 3.5의 APF로 1분간 처리), 2군(pH 3.5의 APF로 30분간 처리), 3군(pH 4.0의 APF로 1분간 처리), 4군(pH 4.0의 APF로 30분간 처리), 5군(pH 7.0의 NaF로 1분간 처리), 6군(pH 7.0의 NaF로 30분간 처리)의 7군으로 분류하였다. 디스크의 표면 거칠기를 측정한 후, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum을 배양하여 디스크에 부착하는 세균의 양을 측정하였다. 결과: 표면 거칠기는 그룹2에서만 유의하게 증가하였다(P < 0.0001). 세균의 부착량은 실험군과 대조군 사이에 유의한 차이를 보이지 않았다. 결론: pH 3.5의 APF를 30분간 처리한 그룹에서 표면 거칠기가 유의하게 증가하였지만, 세균의 부착에 대해서는 유의한 차이를 보이지 않았다.

• 지역사회간호학회지
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• 제1권1호
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• pp.465-502
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• 1989

The purpose of this study was to find out the general characteristics of school health teachers, the status of school health resources and the degrees of self-confident performance for the 124 school health teachers in Pusan City. Data was collected by means of questionaires from Aug. 1986 to Mar. 1987. The data were analyzed by use of percentage, mean, standard deviation, t-test, ANOVA and Pearson-correlation coefficient. The results of this study were as follows: 1 . General Characteristics of School Health Teachers (SHT) 1) The average of age of the SHT was 32.8 years old and 39.5% of them were from 30 to 34 years old. 2) The average for school nursing experience of the SHT was 7.9 years and 37.9% of them were from 5 to 9 years. 3) The 45.2% of them have not the clinical experience. 4) The 74.2% of them were graduated from the 3 years college of nursing. 2. Status of school health resources and nursing activities. 1) The average of student number was 2497.3 and class number was 45.2. 2) The average of school health budget was 1039000 won and 27.7% of school health budget expended on examination cost. 3) Only 29.0% of all schools have organization for school health. 4) The 84.7% of all schools have health clinic separately and 69.1% of schools have less than $33m^2$ sized. 5) The average of clinic visitor number was 2111.8 for 1 year. 6) Major problem was on digestive system. And other problems were skin, respiratory, musculo-skeletal system and dental problem. 7) The number of literal message was 14.4 times for 1 year. 3. The degree of the school health teachers' self-confidence. The school health teachers' self-confidence was deviced into 6 and the maximum degree was 4. 1) Program planning & evaluation; 2.8 2) Clinic management; 2.9 3) Health education, 3.0 4) Management of school environment; 2.7 5) Health care services; 2.7 6) Operating of school health organization; 2.4 4. Significances to the degree of self-confidence on school health nursing activities. 1) There was significant difference between clinical management and Religion (t=2.15 p<.05) 2) There was significant difference between Operating of school health organization and level of school (F=3.588 p<.05) 3) Program planning & evaluation: expending time for clinical management (r=-0.184 p<.05) expending time for health care services (r=0.273 p<.01) 4) Clinical management: use of separate health clinic (r=0.151 p<.05) 5) Health education: use of separate health clinic (r=0.170 p<0.5) 6) Health care services: No. of student (r=-0.144 p<0.5) No. of class (r=-0.160 p<.05) 5. The degree of the school health teachers' self-discipline. The school health teachers' self-discipline was devided into 2 and the maximum degree was 2. 1) Program planning & evaluation:1.8 2) Clinic management: 1.9 3) Health education: 1.9 4) Management of school environment: 1.7 5) Health care services: 1.8 6) Operating of school health organization.: 1.3 6. Significances to the degree of self-discipline on school health nursing activities 1) Program planning & evaluation; Level of nursing education (F=4.309 p<.01) 2) Clinical management: Level of nursing education (F=3.587 p<.05) 3) Operating of school health organization: School health organization (t=-2.68 p<.01) 4) Health care services: School health organization (t=2,58 p<.05) 5) School health performance: School health organization (t=2.32 p<.05) 6) Program planning & evaluation: School health experience (r=0.239 p<.01) Expending time for program planning & evaluation (r=-0.172 p<.05) 7) Clinic management: School health experience (r=0.249 p<.01) Expending time for dinic management (r=0.181 p<.05) No. of student (r=-0.158 p<.05) Expending time of program planning & evaluation (r=-0.199 p<0.5) 8) Health education: School health experience (r=0.234 p<0.1) Expending time of program planning & evaluation (r=-0.193 p<.05) 9) Management of school environment: Age of school health teacher (r=0.142 p<.05) School health experience (r=0.255 p<.01) 10) Operating of school health organization: Medicine Purchase (r=-0.163 p<.05) 11) Health care services: School health experience (r=0.148 p<.05) Medicine purchase (r=-0.229 p<.01) 12) Total school health performance: School health experience (r=0.200 p<.05) Medicine purchase(r=-0.168 p<.05) Based on the above results, the suggestions are as follows: 1) As the SHT take charge of the reasonable number of student, the students will have benefit of the good health service in quality. 2) It is recommended to use the health clinic separately and to arrange adequate place for good school health service. 3) It is necessary that the SHT participate budget for school health. 4) It is required to enhance self-confidence on school health nursing activities through continuous educational programs.