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Effect of fluoride-containing gel on the roughness of a titanium surface and the promotion of bacterial growth

불소함유 겔이 티타늄 표면의 세균성 바이오필름 성장에 미치는 영향

  • Kim, Sun-Jin (Department of Periodontology, Research Institute for Oral Sciences, College of Dentistry, Gangneung-Wonju National University) ;
  • Lee, Jae-Kwan (Department of Periodontology, Research Institute for Oral Sciences, College of Dentistry, Gangneung-Wonju National University) ;
  • Chang, Beom-Seok (Department of Periodontology, Research Institute for Oral Sciences, College of Dentistry, Gangneung-Wonju National University) ;
  • Lee, Si-Young (Department of Oral Microbiology, Research Institute for Oral Sciences, College of Dentistry, Gangneung-Wonju National University) ;
  • Um, Heung-Sik (Department of Periodontology, Research Institute for Oral Sciences, College of Dentistry, Gangneung-Wonju National University)
  • 김선진 (강릉원주대학교 치과대학 치주과학교실) ;
  • 이재관 (강릉원주대학교 치과대학 치주과학교실) ;
  • 장범석 (강릉원주대학교 치과대학 치주과학교실) ;
  • 이시영 (강릉원주대학교 치과대학 미생물학교실) ;
  • 엄흥식 (강릉원주대학교 치과대학 치주과학교실)
  • Received : 2015.09.21
  • Accepted : 2016.03.18
  • Published : 2016.03.31

Abstract

Purpose: The aim of this study was to evaluate whether fluorides at various pH cause changes in the surface roughness of titanium implants that alter the adherence of bacterial biofilms. Materials and Methods: The titanium disks were assigned randomly to the following seven groups according to the fluoride agents and application time (1 minute or 30 minute) used: control (no treatment); group 1 (1.23% acidulated phosphate fluoride [APF] at pH 3.5 for 1 minute); group 2 (1.23% APF at pH 3.5 for 30 minute); group 3 (1.23% APF at pH 4.0 for 1 minute); group 4 (1.23% APF at pH 4.0 for 30 minute); group 5 (2% NaF gel at pH 7.0 for 1 minute); group 6 (2% NaF gel at pH 7.0 for 30 minute). The surface roughness of the titanium disks and the amount of adherent bacteria were measured. Results: Group 2 showed a significantly greater surface roughness than the control group (P < 0.0001). No significant differences in the amount of surface bacteria were observed between the treated samples and the controls. In addition, there were no significant differences in bacterial adherence relative to the incubation period between the treated samples and the controls. Conclusion: The surface roughness of the titanium disks was significantly greater after treatment with APF at pH 3.5 for 30 min compared with that of the controls. In addition, we found that the amount of Porphyromonas gingivalis, Fusobacterium nucleatum, and Aggregatibactor actinomycetemcomitans was similar among all groups

목적: 이번 연구는 다양한 pH의 불소 제재들이 티타늄 표면 거칠기에 미치는 영향을 평가하는 것이었다. 연구 재료 및 방법: 기계절삭형 티타늄 디스크를 시중에서 유통되는 세 가지 불소겔로 처리하였다. 불소겔의 종류와 처리 시간에 따라, 대조군, 1군(pH 3.5의 APF로 1분간 처리), 2군(pH 3.5의 APF로 30분간 처리), 3군(pH 4.0의 APF로 1분간 처리), 4군(pH 4.0의 APF로 30분간 처리), 5군(pH 7.0의 NaF로 1분간 처리), 6군(pH 7.0의 NaF로 30분간 처리)의 7군으로 분류하였다. 디스크의 표면 거칠기를 측정한 후, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum을 배양하여 디스크에 부착하는 세균의 양을 측정하였다. 결과: 표면 거칠기는 그룹2에서만 유의하게 증가하였다(P < 0.0001). 세균의 부착량은 실험군과 대조군 사이에 유의한 차이를 보이지 않았다. 결론: pH 3.5의 APF를 30분간 처리한 그룹에서 표면 거칠기가 유의하게 증가하였지만, 세균의 부착에 대해서는 유의한 차이를 보이지 않았다.

Keywords

References

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