• 혜화의학회지
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• 제14권1호
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• pp.1-4
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• 2005

Objectives : The aim of this study was the collection of dietary treatments of atopic dermatitis Methods : We surveyed the western and oriental medical book concernig the atopic dermatitis. Results : The 20~30% of patients with atopic dermatitis have hypersensitivity reaction on the major food antigen. The food restriction to prevent allergy reaction need to take effect early during infancy and childhood. In oriental medicine, a heat-poison(熱毒) caused by food affects a fetus of pregnancy. So the food, the regimen and the harmony of the five tastes is stressed the need. When certain foods are restricted to prevent allergy reaction, infant and children with food related Atopic Dermatitis need extra dietary efforts to maintain optimal nutrition as they are in the growth period.

• 운동영양학회지
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• 제13권1호
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• pp.1-7
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• 2009

It has been shown that both caloric restriction and exercise, enhances glucose uptake through translocation of GLUT-4 protein. It remains unclear how exercise and caloric restriction affect the changes in VAMP (vesicle-associated membrane protein) in skeletal muscle and GLUT-2 in liver. This study investigated the effects of exercise training and caloric restriction on the expressions of glucose transport relating proteins in muscle and liver tissues in diabetic rats. Forty male Sprague-Dawley rats (250±10 g; 8 week in age) were assigned equally to four different groups; control (C), exercise only (E), dietary restriction only (D) and dietary restriction and exercise (DE). Daily food consumption was monitored to establish baseline intake. Both C and E groups consumed baseline food intake while D and DE groups were provided with only 60% of baseline total food intake. Forty-eight hours after intraperitoneal injection of STZ (50 mg/kg), diabetes was confirmed (8-hr fasting blood glucose levels ≥300 mg/dl). Rats in the E and DE groups exercised on a motorized treadmill for 30 min/d, 5 days/week for 4 weeks (5 min running at 3 m/min, 0% grade; 8 m/min for the next 5min, and then 15 m/min for 20 min). Rats were sacrificed 48 hrs after the last bout of exercise. Soleus muscle and liver were extracted to analyze for GLUT-4, VAMP-2, and GLUT-2, respectively. All variables were analyzed using the Western Blotting technique. All values were expressed as optical volume measured by optical density. A Two-way ANOVA was used to examine the difference between groups and applied Duncan's test for post-hoc. No significant differences in GLUT-2 expression were found among groups. However, E (280133±13228 arbitrary units{AU}) and DE (268833±14424 AU) groups showed significantly higher (p<.001) levels of GLUT-4 as compared with C (34461±2099 AU) and D groups (27847±703 AU). VAMP-2 protein expression increased (p<.001) in E (184137±7803 AU) and DE (189800±10856 AU) groups as compared to C (74201±8296AU) and D (72967±863 AU) groups. These results suggest that either exercise with or without caloric restriction increases the up-regulation of GLUT-4 and VAMP-2 in skeletal muscle of diabetic rats. However, GLUT-2 protein in liver was not affected by either exercise or exercise with caloric restriction.

• BMB Reports
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• 제56권10호
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• pp.537-544
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• 2023

The share of the population that is aging is growing rapidly. In an aging society, technologies and interventions that delay the aging process are of great interest. Dietary restriction (DR) is the most reproducible and effective nutritional intervention tested to date for delaying the aging process and prolonging the health span in animal models. Preventive effects of DR on age-related diseases have also been reported in human. In addition, highly conserved signaling pathways from small animal models to human mediate the effects of DR. Recent evidence has shown that the immune system is closely related to the effects of DR, and functions as a major mechanism of DR in healthy aging. This review discusses the effects of DR in delaying aging and preventing age-related diseases in animal, including human, and introduces the molecular mechanisms that mediate these effects. In addition, it reports scientific findings on the relationship between the immune system and DR-induced longevity. The review highlights the role of immunity as a potential mediator of the effects of DR on longevity, and provides insights into healthy aging in human.

• 한국도서관정보학회지
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• 제49권3호
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• pp.33-53
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• 2018

많은 공공도서관은 다양한 이유를 근거로 음식물 반입을 제한(또는 금지)하고 있는데, 그것이 정당한지 아니면 부당한지를 논증할 필요가 있다. 이에 본 연구는 공공도서관을 이용하는 대구시민 485명을 대상으로 음식물 정책에 대한 인식을 조사하고 그 결과를 바탕으로 다양한 관점에서 개선방향을 제시하였다. 첫째, 사회 사조 측면에서 음식물 제한정책에 대한 전향적인 재검토가 필요하다. 둘째, 이용자 기본권 보장 차원에서는 이용자의 음식물 반입을 허용하는 것이 공공도서관의 존재가치와 사회적 역할을 정당화하고 생태계를 강화하는데 유리하다. 셋째, 장서 및 시설공간의 이용촉진 측면에서 음식물 허용에 따른 역기능보다 순기능을 강조하는 것이 바람직하다. 넷째, 자치조례나 도서관 규정에 모든 또는 대다수 음식물을 제한하는 전체주의적 사고는 지양되어야 한다. 마지막으로 공공도서관은 음식물 반입을 허용하되, 공간별로 섭취 가능한 종류를 규정하고 이용자에게 적극적으로 홍보할 필요가 있다.

• 한국식품영양과학회지
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• 제27권6호
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• pp.1160-1165
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• 1998

When total cellular DNA was isolated from Porphyra tenera by ultracentrifugation on Hoechst dye/CsCl gradients method, plasmid like DNA's were concentrated at the upper band which were characterized with a A＋T rich organelle DNA's in the CsCl gradients. Based on their electrophoretic migration in different concentration of agarose gel, buffer system, and electric power etc. and the results of restriction digestion, the plasmid like DNA's were concluded to have circular conformation. This is the first report of putative circular plasmid DNA from the P. tenera, which is a autonomously replicating plasmid existing with a high copy number plasmid in the cell. The minimum size of this plasmid estimated by restriction endonuclease digestion was appeared to be 2.5kb in size.

• Journal of Nutrition and Health
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• 제46권5호
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• pp.410-417
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• 2013

본 연구는 만 19~60세의 BMI $23kg/m^2$ 이상 여성 61명을 대상으로 12 주간 열량 섭취 제한 시 체중 감량에 영향을 미치는 요인을 분석하고 이들의 상관성을 알아보고자 하였다. 12주간의 열량 섭취 제한으로 하루 평균 451.9 kcal가 감소하였고 열량 제한 후 체중은 평균 4.5% 감소하였고, 내장지방량은 11.9%, 피하지방량은 8.2% 감소하였으며, 혈압도 유의하게 감소하였다 (p < 0.01). 혈액지표 중 GPT 농도는 감소하였으며 (p < 0.05), 아디포넥틴 농도는 증가하였다 (p < 0.01). 연령이 증가함에 따라 섭취 열량 감소량이 낮게 나타났다 (r = 0.31, p = 0.02). 젊은 연령에서 열량 섭취량 감소가 컸으며, 연령이 증가할수록 열량 섭취량 감소가 낮게 나타났다. 연령은 독립적으로 신체 조성 변화와 유의한 관련이 있었으며 (p < 0.05), 연령이 증가함에 따라 열량 섭취 제한에 따른 체중 및 내장지방과 피하지방의 감소량이 낮았으며, 특히, 내장지방 감소량이 낮게 나타났다 (p < 0.05). 또한, 연령이 증가함에 따라 열량 섭취 제한에 따른 렙틴 농도 감소량도 낮았다 (p < 0.05).

• 한국미생물·생명공학회지
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• 제15권6호
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• pp.436-440
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• 1987

앞서 보고한 바와 같이 토양으로부터 분리한 K. pneumoniae NFB-320의 pullulanase 유전자를 pBR 322을 이용하여 E. coli에 cloning한 결과 약 14.4kb 의 재조합 plasmid DNA pYKL451을 얻었다. 이러한 pullulanase 유전자에 대한 유전적 정보를 얻기 위해 여러 가지 제한효소로 단일 혹은 이중 절단을 행하여 삽입된 pullulanase 유전자의 제한효소 절단지 도를 작성하였으며, E. coli(pYKL451)과 K. pneumoniae NFB-320이 생산하는 pullulanase의 효소적 특성을 조사하였다. 생산되는 두 균주의 효소는 50-55$^{\circ}C$ 부근에서 최적온도를 나타냈으며 최적pH는 모두 6.0이었다. 효소 안정성에 미치는 pH의 영향은 4$0^{\circ}C$에서 90min 간 방치했을 때 pH 5.0-10.0에서 안정하였으며 열안정성은 (pH6.0) 각 온도에서 한시간 처리하였을 때 4$0^{\circ}C$까지는 안정하였으나 5$0^{\circ}C$ 이상에서는 효소의 활성이 급격히 감소하였다.

• Nutritional Sciences
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• 제7권4호
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• pp.189-195
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• 2004

1bis study investigated the antioxidative effect in kidney of senescence-accelerated prone SAMP8 mice with calorie restriction. 4-weeks-old SAMP8 female mice were divided into 4 groups according to the experimental feeding period: for 4, 8, 12 month, and at natural death. Each group was subdivided into 2 groups, with thirteen mice each one, as ad libitum group and as dietary restriction group (60% of ad libitum feeding amount). After feeding for a given period, the mice were sacrificed to get the following results: among the experimental groups, there wereno significant differences in xanthine oxidase (XOD) activity in their kidney tissues. The contents of cytochrome $P_{450}$ decreased in ad libitum group and dietary restriction group by age. The activity of NADPH-cytochrome $P_{450}$ reductase showed a trend similar to cytochrome $P_{450}$. Superoxide radical content increased with age. At the 4th, 8th and 12 months of the experimental period, the activity in the dietary restriction group was less than that of ad libitum group by as much as 17% 14% and 14% respectively. For hydrogen peroxide, the contents were increased in the ad libitum group with age, while no correlation between content and age was observed in the dietary restriction group. In the 8th and 12th months of the experimental period, the were in the dietary restriction group less than that of ad libitum group counterpart as much as 17% and 20o/c, respectively. For the cellular membrane stability of the kidney, no significant correlation with age was observed in either the dietary restriction group or the ad libitum group. However at the 12th month of the experiment, however, the stability in the dietary restriction group was 11 % higher than that in the ad libitum group. In conclusion, with these results obtained from the SAMP8 mouse model, we demonstrate that dietary restriction has the effects of anti-oxidation and anti-senescence in the kidney.

• 대한지역사회영양학회:학술대회논문집
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• 대한지역사회영양학회 1999년도 추계학술대회
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• pp.656.2-657
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• 1999

• Journal of Nutrition and Health
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• 제14권4호
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• pp.151-161
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• 1981

The objective of this study was to compare the electrolytes contents in skeletal muscles of two different groups of rats, one for control fed 20% casein diet, and the other experimental group recovered from 7% casein diet as protein restriction feeding for two weeks at the beginning of this experiment. Two different comparison criteria were used in this study, one for comparison between animals at the same age groups and the other between groups weighing same body weight as the control groups. Food consumption of animals for experimental and control groups were ad libitum. Following measurement and samples were collected: body weight, five different muscles-anterior tibialis (A.T.) extensor digitorium longus (E.D.L.) soleus, plantaris, gastrocnemius-for electrolytes and protein analysis. The experimental groups showed lower body weight than that of control group. Same trend was showen in protein content in five different muscles. Magnesium and potassium content in four muscles except gastrocnemius showed lower and sodium higher in experimental groups than those in control group at fourteenth day, after recovery from protein restriction, electrolytes content change showed differently in four muscles. Magnesium and potassium contents were increased as protein content in diet. Sodium content decreased as one week intake of recovery diet started, thereafter it was rather slowly increased. Phosphorus content in gastrocnemius at the second week in experimental group was slightly lower than that of control group, and it recovered rapider while at the same body weight group it was higher in experimental group.