• Microbiology and Biotechnology Letters
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• v.40 no.2
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• pp.92-97
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• 2012

Antimicrobial peptides (AMPs) are important components of living organisms acting against Gram-negative and Gram-positive bacterial and fungal pathogens. Cathelicidin human peptides have a variety of biological activities that can be used in clinical applications. AMPs are not produced naturally in large quantities, and chemical synthesis is also economically impractical, especially for long peptides. Therefore, as an alternative, heterologous expression of AMPs by recombinant techniques has been studied as a means to reduce production costs. E. coli is an excellent host for the expression of AMPs, as well as other recombinant proteins, because of the low cost involved and its easy manipulation. However, overexpression of AMPs in E. coli has been shown to cause difficulties resulting from the toxicity of the subsequently produced AMPs. Therefore, fusion expression was theorized to be a solution to this problem. In this study, AMPs were expressed as fused proteins with the glutathione S-transferase (GST) binding protein to protect against the toxicity of AMPs when expressed in E. coli. The LL37, and hybrid gaegurin and LL37 (GGN4(1-16)-LL37(17-32), which we designated as GL32, peptides were expressed as GST-fusion proteins in E. coli and the fusion proteins were then purified by affinity columns. The purified peptides were obtained by removal of GST and were confirmed by western blot analysis. The purified antimicrobial peptides then demonstrated antimicrobial activities against Gram-negative and Gram-positive bacterial strains.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.7
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• pp.73-83
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• 2017

This study was conducted to evaluate the chemical composition, digestibility, and energy value of 15 non-conventional feeds produced in South Korea as ruminant feeds. Three Hanwoo steers (body weight, $520{\pm}20.20kg$) fitted with a permanent rumen cannula and duodenal cannula were housed individually in tie-stall barns, followed by a 14-day adaptation period and 3-day experimental period. Chemical composition analysis, in situ nylon bag, and mobile bag technique were used as experiments. As a result of the chemical composition analysis offeeds, crude protein (CP) contentsofmalt meal, perilla meal, soy sauce cake, and soymilk residue were greater than 30%. As a result of the degradability characteristics analysis of feeds using an in situ nylon bag, rumen undegraded protein (RUP) contents of beet pulp, brewer's grain, coffee meal, malt meal, milo bran, perilla meal, ramen residue, and soymilk residue were greater than 50%. Analysis of total digestible nutrient (TDN) values of feeds using an in situ mobile bag showed that TDN values of beet pulp, brewer's grain, makgeolli residue, milo bran, perilla meal, ramen residue, rice bran, soy sauce cake, soybean curd cake, soymilk residue, and wheat bran weregreater than 50%. In summary, these non-conventional feeds have high potential value as good feed resources to replace formulated feeds or roughage. Therefore, the chemical composition, digestibility, and energy value of non-conventional feeds obtained from this study can be used as base data for the manufacture of ruminant total mixed ration (TMR) with improved feed efficiency, reduced feed costs, and reduction of environmental pollution.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.7
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• pp.481-491
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• 2016

This study analyzed the customer's revisit concepts and storytelling by restaurant customers to identify the elements that can attract the attention of customers. The restaurant concept or advertising, restaurant decor will also have to change to emphasize the comparative advantage of the features in the menu of a restaurant or other restaurants / service that tells a story. Membership cards or money, or the same convenience and use of the restaurant non-monetary 'transition cost' can compensate for the negative emotions and low satisfaction level of restaurant customers and help them choose to revisit the restaurant. Therefore, if such a transition takes full account of the effects of the cost to the customer, it can be used as an effective means. In class restaurants, such as the food and customer service, the increased levels of the restaurant atmosphere and empirical elements, such as store concept and physical environment, can improve the positive consumer sentiment, strengthen the customer satisfaction and have a positive effect on the customers' revisit intention. It is also important to improve the level of visual texture using light. In addition, positive consumer sentiment can be induced using the store concept, the physical environment, and experiential elements. In other words, membership cards, mileage points, and various financial and non-financial inducements as a marketing tool will have a positive impact on the customer's revisit intention.

• Resources Recycling
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• v.24 no.3
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• pp.16-26
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• 2015

This study aims to suggest possible policy options for collection of post-consumer diapers as a sustainable prerequisite to implementation of public recycling system with the desktop monitoring results and analysis of collection cost involved. By conducting a diaper collection pilot test on daycare centers and households in the Seoul Nowon-gu, this study revealed that the odor issues did not stand out as an important hindering factor and that the collection rate was the largest contributing factor regarding collection costs. The exploratory cost analysis result shows that the implemented recycling system must guarantee collection rate over 50% via twice-a-week collection frequency, to be comparable or even superior to the current food waste collection cost. Also the pilot demonstration test showed that the final collection rate can be achieved as much as over 50%. The promising data thus obtained cost economically viable future diaper recycling system implementation in the entire Seoul metropolis and adjacent areas of dense population. With the concomitant development of recycling technology and related infrastructure for systematic collection of diaper waste, it makes our society much more sustainable.

• Korean Journal of Agricultural Science
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• v.10 no.2
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• pp.393-405
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• 1983

The demand for livestock and poultry products including beef, pork, chicken, egg and milk whose income elasticities are relatively higher than other staple foods, has been increased significantly during the past two decades in response to the remarkable increase in per capita GNP. This trend will be continued during the fifth and the sixth five year economic development plan period beginning with 1982. The annual GNP growth rate will be 7.5% on the average during the next 10 years. It is greatly needed to estimate the demand for beef, pork, chicken egg and milk and to study the feasibilities of domestic production of livestock products for the formulation of adequate policies in order to equate the consumption and the production during the 1980s. So this study reviewed the possible changes in the food consumption patterns during the 1980s, estimated the demand for beef, pork, chicken, egg and milk by using empirical demand functions and finally made suggestions for the formulation of long term price stabilization policies for each livestock, poultry and dairy products through the equilibrium of the quantity of demand for and supply of the products. There are many factors affecting the demand for meats, but this study considered own price, prices of supplements and substitutes and per capita income as the independent variables in the demand equations. It was found that it's own price and income were most significantly affecting factors among others and the degree of substitution effects were remarkably different among the products. According to the meat demand derived in this study, per capita consumption of beef, pork and chicken in the base year 1982 was 11.2kg for total meat, 2.5kg beef, 6.0kg pork and 2.5kg chicken, 106 pieces egg, 15.1kg milk respectively, while those in 1991 were 19.3kg for total meat, 4.8kg beef, 9.6kg pork, 4.9kg chicken, 133pieces egg and 44.1kg milk. It is also predicted through this study that, when the level of production costs be maintained, the domestic production of pork and chicken will meet the demand for them during the fifth and sixth five year economic plan period. However, there will be chronic shortage of beef supply during the coming years. The annual import requirement will be 30,000tons to 40,000tons during the period. In order to stabilize the domestic livestock and poultry and dairy products market, the government should introduce measures to curb the increase in beef consumption by encouraging the consumption of pork and chicken. For this, the livestock production policy measures should be concentrated on : 1) the improvement of infrastructures of beef production by introducing advanced feeding and management technology, subsidies for the establishment of facilities and price support programs for farmers : 2) the development of dairy beef : 3) the reinforcement of the forecast systems for pork and chicken production and consumption.

• Korean Journal of Organic Agriculture
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• v.20 no.4
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• pp.503-518
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• 2012

Korean type of no-tillage cultivation method which was applied on this study used the ridge and the furrow and constantly recycling them as it was suitable for Korea's weather and farming conditions. This no-tillage cultivation was reported to have little negative impact such as reduction of production (Kwon et al., 1997). In addition, it was found to have a lot of benefits as it requires less agro-materials and energy costs as well as shortened working hours because tillage operation is not needed. (Yang et al., 2012). According to an analysis, no-tillage cultivation can reduce greenhouse gas emissions by $344.7kgCO^2$ (58%) in every 10a ($1,000m^2$) compared to ordinary pepper farming technique (Korea averages). Direct-indirect reduction effects from using fertilizer and using less amount of energy were 92% and 44% respectively both of which can be considered very high. Besides the direct effects of no-tillage cultivation, soil management using no-tillage technique raises carbon sequestration effect on soil as time goes on (West & Marland, 2002), that is why the technique is expected to have constant carbon emission reduction effect. For theses reasons, distribution and expansion of Korean type no-tillage cultivation are expected to play a role as major agro-green technologies for achieving our goal of reducing greenhouse gas emissions in agricultural sector.

• Culinary science and hospitality research
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• v.17 no.2
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• pp.51-73
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• 2011

Companies are required to invest in numerous intial marketing costs for establishing brand power, thus, many companies in the foodservice industry are increasing their budgets on communication to strengthen their own brand equity components such as brand recognition, brand royalty, perceived quality and associated image using mass media that has not been used before. For this study, 617 samples were analyzed by such analytic methods as a frequency analysis, cross-tabulation, a factor analysis and a reliability analysis for validity, and a confirmatory factory analysis, a model adequacy evaluation, and a path analysis using AMOS 17.0. The hypothesis verification by the AMOS analysis has revealed that all hypotheses positively influenced contract foodservice brands, and the brand recognition of a university contract foodservice company has an impact on sincerity and refinement. Therefore, positive brand image formation encourages strong brand establishment between a university contract foodservice business and customers, and this study strongly suggests continuous further studies about constructing a positive brand image.

• Korean Journal of Agricultural Science
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• v.39 no.2
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• pp.271-277
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• 2012

Dimensions, operation conditions and improvement items for round roof windows were investigated in arch shape single-span plastic greenhouse with roof vents, and natural ventilation performance was analyzed based on the ventilation theory. Diameter of round roof windows was mostly 60 cm, and chimney height projected on roof was average 30 cm. Installation space was mostly 5 to 6 m but farmhouse of 10 m and over was 16.7% also. A round roof window which has 60 cm diameter was installed to 6 m space generally and 80 cm diameter was installed to 10 m space, but correct standards did not exist. There were a lot of opinions that ventilation effect of round roof windows is fairly good and user satisfaction is generally excellent. It is problem that there is few effects in summer and that vinyl around each vent tears well and rainwater leaks, and improvement hope item required development of automatic control system. In the wind speed of 0.3 m/s, it was estimated that natural ventilation rates were 0.69, 0.55, 0.50 and 0.48 volumes per minute in case of 2, 4, 6 and 8 m installation space for round roof windows, respectively. It was analyzed that the ratio of ventilation due to buoyancy out of total ventilation were 65.2, 41.9, 29.9 and 22.8% in case of 2, 4, 6 and 8m installation space, respectively. By the round roof windows installed at space of 6 m, ventilation rate was estimated to 0.5 volumes per minute, and we can expect the increase in ventilation rate of 30%. In order to meet the recommended ventilation rate for summer season, we have to install the round roof windows at space of 1 to 2 m. However, it is difficult to apply those installation space because of falling productivity due to lower light transmittance as well as rising costs. It is estimated that the installation space of 6m is appropriate for spring or fall season. Therefore it is necessary to encourage installing the roof windows in single-span plastic greenhouses.

• Korean Journal of Plant Resources
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• v.19 no.5
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• pp.606-611
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• 2006

Decay during soybean sprout culture detracts their quality as well as increases their production costs. This study was done to determine the effects of acetic and propionic acids on growth and morphological characters of the sprouts. The soybean seeds of 3 cultivars (cv. Eunhakong, Pungsannamulkong and Orialtae) imbibed for 2 minutes at their different concentrations (0, 0.1 and 0.2%) were soaked for 6 hours in 4 ppm BA solution after the first 5.5 hour water imbibition and 0.5 hour aeration, and cultured at $20^{\circ}C$. On the 6th day, harvested soybean sprouts were classified into 4 categories on the base of hypocotyl length;>7cm, 4 to 7cm,<4cm and not germinated, and their morphological characters, fresh weights, lesion spots on cotyledons were measured or analyzed. The stronger concentration the higher rate of longer than 4cm although there was no significant difference between the two acids. Pungsannamulkong showed the longest hypocotyls but Eunhakong did the thickest ones, and Orialtae did the greatest total fresh weight, in which all the characters were not influenced by the two acids and their concentrations. Lesion spots on the cotyledons were equal to the two acids although less in their treatments than in no treatment. Utilization of propionic acid was more desirable Dan acetic acid through their treatment cost analysis.

• Culinary science and hospitality research
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• v.19 no.3
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• pp.57-72
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• 2013

This study carried out an empirical analysis on the long-term effects of a marketing communication strategy used as a means of promotion(advertisement, free gift) of Christmas cake despite its high costs. Such verification results were considered to provide as important core elements for successful management in the bakery industry. By utilizing SPSS and AMOS, exploratory factor analysis(EFA) and confirmatory factor analysis(CFA) were conducted for the validity of the measurement items, and Structural Equation Model(SEM) was performed to verify the hypothesis. The analysis result is as follows. First, advertisement attribute(${\beta}$=0.269, t=4.512, p,<0.001) and free gift attitude(${\beta}$=0.293, t=5.043, p<0.001) had a positive influence on trust. Second, trust had a positive influence on purchase intention(${\beta}$=0.513, t=8.677, p<0.001). Third, advertisement attribute(${\beta}$=0.104, t=1.966, p,<0.05) and free gift attitude(${\beta}$=0.204, t=3.817, p<0.001) had a positive influence on purchase intention. Lastly, advertisement attitude appeared different by advertisement types(actors and animation), and ads with an actor were evaluated high than animation ads. Also, gift attitude appeared different by gift types(speaker doll/character hat and turtleneck muffler), and a turtleneck muffler was evaluated high than a speaker doll or a character hat.