• 한국축산식품학회지
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• 제33권2호
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• pp.244-250
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• 2013

본 연구는 염지촉진 방법인 인젝션 및 텀블링 처리에 따른 한우 우둔 염지육의 품질에 미치는 영향을 알아보고자 하였다. 처리구는 인젝션과 텀블링 단일 처리구 및 복합처리구로 텀블링 처리구의 경우 진공과 비진공으로 나누었으며 대조구는 6시간 침지하였다. pH는 5.26-5.51의 범위로 인젝션 후 텀블링 처리구가 5.51로 가장 높았고 텀블링 단일 처리구에서 5.26으로 가장 낮았으며 인젝션 및 텀블링 복합처리구에서 높은 경향을 나타내어(p<0.05) 보수력 증진에 가장 큰 영향을 미치는 것으로 판단되었다. 염도와 당도 측정결과 pH가 높게 측정된 인젝션 및 텀블링 복합처리구에서 높은 경향을 나타내어 염지 촉진효과가 가장 우수한 것으로 나타났다. 가열감량은 대조구에서 22.08%로 가장 많았으나 인젝션 후 텀블링 진공 처리구와 비진공 처리구 및 텀블링 진공처리구간의 유의적인 차이를 보이지 않았으며 인젝션 처리구는 13.98%로 유의적으로 가장 적게 나타났다. 조직감 측정 결과 hardness는 8.01-13.99 kg의 범위로 인젝션 후 진공텀블링 처리구가 가장 낮았으며 대조구에서 가장 높게 나타났다(p<0.05). 그러나 인젝션 후 진공 및 비진공 텀블링 처리구가 텀블링 진공 및 비진공 처리구보다 낮았지만 유의적인 차이를 나타내지 않았다. 또한 인젝션 처리구의 경우 단일 텀블링 진공 및 비진공 처리구와 비교할 때 텀블링과 인젝션 처리는 연도향상에 유사한 영향을 주는 것으로 보이며 단일 텀블링 진공과 비진공 처리구 및 인젝션 후 진공 및 비진공 텀블링 복합 처리구의 결과로 볼 때 진공과 비진공의 차이 또한 없는 것으로 판단되었다. 색도는 염지 및 가공방법에 따른 가열 전후의 뚜렷한 경향을 보이지 않았다. 관능평가 결과 모든 항목에서 인젝션 및 텀블링 공정으로 제조한 처리구가 대조구에 비해 유의적으로 높게 평가되었으며 연도정도, 조직감의 기호도 및 맛의 영향으로 종합적인 기호도에서 인젝션 후 비진공 텀블링 복합 처리구가 가장 높은 기호도를 나타내었다. 따라서 본 연구결과 인젝션 및 텀블링 공정은 육의 가공에 있어 가열수율, 연도 및 관능적 기호도 향상에 긍정적인 영향을 주는 것으로 사료되었다.

• 한국식품영양과학회지
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• 제35권10호
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• pp.1456-1460
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• 2006

변질된 팥앙금에서 분리한 균주는 광학 및 전자현미경을 통하여 관찰한 결과 그람양성 간균으로 포자를 형성하고 형성된 집락은 원형으로 점질성의 물질을 생산하는 mucoid type이었으며, 표면은 smooth 하였고 광택을 나타내었다. 미생물 동정 시스템($MicroLog^{TM}$ System, Release 4)인 VITEK 2 COMPACT을 이용하여 분리된 균주의 46개의 생화학적 분석을 비교한 결과 팥앙금의 부패 원인균은 Bacillus subtilus로 동정 (97.0%)되었으며, 보다 더 정확한 동정을 위하여 부패균의 세포벽 지방산 조성을 분석하여 Midi sherlock시스템의 databank와 비교 분석시 그 유사성이 0.724로 분석되었다. Bacillus subtilus의 포자의 D-value는 $115^{\circ}C$에서의 4.85분이 었고, $121^{\circ}C$에서는 0.69분, $125^{\circ}C$에서는 0.48분이었으며, Z-value는 $9.71^{\circ}C$이었다. $45^{\circ}C$에서 수분활성도에 따른 분리균의 생육 상태를 관찰한 결과 수분활성도 0.92이하에서는 균이 증식되지 않았고, 0.94에서는 증식되는 것으로 관찰되었다.

• Journal of Nutrition and Health
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• 제39권2호
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• pp.109-114
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• 2006

The effects of the erect bipedal stance exercise on bone mass and the biomarkers of bone formation and resorption were investigated in rats. Five-week old rats were assigned into control and exercise groups. The rats of exercise group were weight-bearing-trained for 13 weeks in the cage designed to adjust progressively the height from 26.5 cm to 31.5 cm to force the rats rising an erect bipedal stance for feeding and drinking. There was no significant difference in food intakes between two groups. But body weight gain was significantly increased in control group. The lengths of femur, tibia, humerus and radius were significantly longer in control group than exercise group, but the femur and tibia weights per body weight were significantly higher in exercise group than control group. Also the breaking force of femur and tibia in exercise group were higher than control group significantly. The calcium contents of femur and tibia were significantly increased in exercise group than control group. The activity of bone specific alkaline phosphatase (B-ALP) and the osteocalcin contents of serum (the biomarkers of bone formation) in exercise group were higher than control group, but the carboxyterminal propeptide of type I procollagen (P1CP) contents of serum did not show any difference between two groups. However the urinary deoxypridinolin (DPD) excretion, biomarker of bone resorption, was significantly lower in exercise group than control group. From these results, it has been indicated that the erect bipedal stance exercise enhanced the density and the strength of femur and tibia by increasing biomarkers of bone formation and suppressing a biomarker of bone resorption in rats.

• 농촌계획
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• 제28권2호
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• pp.41-50
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• 2022

The primary purpose of this study is to identify various factors that affect farmland prices according to changes in the actual transaction price of farmland over the past decade, and to use this to derive policy implications for price stabilization. To this end, the farmland price model are constructed at the parcel level in the case area (Namwon-si, Jinju-si). The analysis method is based on the Hedonic price function, and the OLS and the quantile regression are used for the parcel level model. As a result of estimating the parcel level farmland price model in the case area, the larger the parcel area, the lower the farmland price, and the higher the farmland price outside the agricultural promotion area. It was found that there was a price difference according to the type of special purpose areas, and the location characteristics showed some differences across the cities. The farmland price models presented in this study are suitable for identifying the factors affecting farmland prices, and are expected to be highly utilized in that it is possible to construct flexible variables suitable for regional characteristics.

• Journal of Microbiology and Biotechnology
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• 제33권3호
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• pp.329-338
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• 2023

Enterohemorrhagic Escherichia coli (EHEC) is a foodborne pathogen that produces attaching and effacing lesions on the large intestine and causes hemorrhagic colitis. It is primarily transmitted through the consumption of contaminated meat or fresh produce. Similar to other bacterial pathogens, antibiotic resistance is of concern for EHEC. Furthermore, since the production of Shiga toxin by this pathogen is enhanced after antibiotic treatment, alternative agents that control EHEC are necessary. This study aimed to discover alternative treatments that target virulence factors and reduce EHEC toxicity. The locus of enterocyte effacement (LEE) is essential for EHEC attachment to host cells and virulence, and most of the LEE genes are positively regulated by the transcriptional regulator, Ler. GrlA protein, a transcriptional activator of ler, is thus a potential target for virulence inhibitors of EHEC. To identify the GrlA inhibitors, an in vivo high-throughput screening (HTS) system consisting of a GrlA-expressing plasmid and a reporter plasmid was constructed. Since the reporter luminescence gene was fused to the ler promoter, the bioluminescence would decrease if inhibitors affected the GrlA. By screening 8,201 compounds from the Korea Chemical Bank, we identified a novel GrlA inhibitor named Grlactin [3-[(2,4-dichlorophenoxy)methyl]-4-(3-methylbut-2-en-1-yl)-4,5-dihydro-1,2,4-oxadiazol-5-one], which suppresses the expression of LEE genes. Grlactin significantly diminished the adhesion of EHEC strain EDL933 to human epithelial cells without inhibiting bacterial growth. These findings suggest that the developed screening system was effective at identifying GrlA inhibitors, and Grlactin has potential for use as a novel anti-adhesion agent for EHEC while reducing the incidence of resistance.

• Journal of Microbiology and Biotechnology
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• 제34권8호
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• pp.1599-1608
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• 2024

Yersinia enterocolitica is a globally distributed food-borne gastrointestinal pathogen. The O-antigen variation-determined serotype is an important characteristic of Y. enterocolitica, allowing intraspecies classification for diagnosis and epidemiology purposes. Among the 11 serotypes associated with human yersiniosis, O:3, O:5,27, O:8, and O:9 are the most prevalent, and their O-antigen gene clusters have been well defined. In addition to the O-antigen, several virulence factors are involved in infection and pathogenesis of Y. enterocolitica strains, and these are closely related to their biotypes, reflecting pathogenic properties. In this study, we identified the O-AGC of a Y. enterocolitica strain WL-21 of serotype O:10, and confirmed its functionality in O-antigen synthesis. Furthermore, we analyzed in silico the putative O-AGCs of uncommon serotypes, and found that the O-AGCs of Y. enterocolitica were divided into two genetic patterns: (1) O-AGC within the hemH-gsk locus, possibly synthesizing the O-antigen via the Wzx/Wzy dependent pathway, and (2) O-AGC within the dcuC-galU-galF locus, very likely assembling the O-antigen via the ABC transporter dependent pathway. By screening the virulence genes against genomes from GenBank, we discovered that strains representing different serotypes were grouped according to different virulence gene profiles, indicating strong links between serotypes and virulence markers and implying an interaction between them and the synergistic effect in pathogenicity. Our study provides a framework for further research on the origin and evolution of O-AGCs from Y. enterocolitica, as well as on differences in virulent mechanisms among distinct serotypes.

• Asian-Australasian Journal of Animal Sciences
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• 제20권6호
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• pp.827-831
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• 2007

Myostatin is a member of the transforming growth factor-${\beta}$(TGF-${\beta}$ super-family. It acts as a negative regulator for skeletal muscle growth. Myostatin mutations are characterized by a visible, generalized increase in muscle mass in double muscled cattle breeds. To understand the biochemistry and physiology of the Chinese Yellow bovine myostatin gene, we report here for the first time expression of the gene in Escherichia coli (E. coli). Primers of the myostatin gene of Chinese Yellow Cattle were designed on the basis of the reported bovine myostatin mRNA sequence (Gen-Bank Accession No. NM005259) and optimized for E. coli codon usage. XhoI and EcoRI restriction enzyme sites were incorporated in the primers, and then cloning vector and expression vector were constructed in a different host bacterium. The expressed protein had a molecule mass of about 16 kDa as determined by SDS-PAGE under reducing conditions. The expressed protein reacted specifically with myostatin monoclonal antibody on immunoblots. Our studies should lead to the investigation of the differences in myostatin genes of various cattle and could benefit human health and food animal agriculture.

• 한국안전학회지
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• 제33권5호
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• pp.127-133
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• 2018

The Saemangeum master plan includes dredging and waterproofing materials, construction projects that can change the hydraulic characteristics of the Saemangeum and Mangyeong and Dongjin River basins. In this study, the river safety of 2030 when the Saemangeum master plan was completed for 100 year frequency, 500 year frequency and 100 year frequency applied RCP 8.5 scenario was examined using Delft3D. As a result of the analysis, it was confirmed that there was no overflowing point at the 100 year frequency, but the difference between the flood level and the river bank elevation was relatively small at the curved and river joint part. At the 100-year frequency with the 500-year frequency and the RCP 8.5 scenario, the possibility of overflowing at several locations was confirmed. The possibility of river bed loss due to fast velocity appears in the upstream part of Mankyung River and it is necessary to monitor the safety of hydraulic structures continuously. In addition, it is expected that the expansion of the area showing the characteristics of the lake due to dredging will affect the sediment mechanism and water quality, so detailed and diverse studies will be needed.

• KSBB Journal
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• 제27권3호
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• pp.161-166
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• 2012

The purpose of this work was to examine the antibacterial activity derived from a lactic acid bacterium isolated from korean rice wine, called makgeolli. Various physiological and biochemical properties of this strain were characterized. Both the BIOLOG system and phylogenetic analysis using 16S rRNA sequencing were utilized for identification, and the strain was designated as Lactobacillus casei HK-9, and registered in GenBank as [JQ951606]. Growth rate, production of organic acids (e.g., lactic acid and acetic acid), and pH changes during growth were monitored. The maximum concentrations of lactic acid and acetic acid were approximately 576 mM and 199 mM, respectively, and pH was changed from 7.00 to 3.74 after 72 h of incubation. HPLC was used to confirm the production of lactic acid and acetic acid. Significant antimicrobial activity of the concentrated supernatant was demonstrated against various food-poison causing bacteria (e.g., Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, Escherichia coli, Salmonella enteritidis). Ethanol tolerance of L. casei HK-9 showed up to 12% of ethanol within the culture.

• Fisheries and Aquatic Sciences
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• 제10권2호
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• pp.60-67
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• 2007

Myostatin (MSTN; also known as GDF8) is a member of the transforming growth factor ${\beta}-superfamily$ of proteins. MSTN negatively regulates mammalian skeletal muscle growth and development by inhibiting myoblast proliferation. Mice and cattle possessing mutant MSTN alleles display a 'double muscling' phenotype characterized by extreme skeletal muscle hypertrophy and/or hyperplasia. We isolated the full-length cDNA of a novel MSTN gene from S. schlegeli muscle tissue and examined its expression pattern in various tissues. The full-length gene (GenBank DQ423474) consists of 1941bp with an open reading frame of 1134 bp, encoding 377 amino acids that show 62-92% amino acid similarity to other vertebrate MSTNs. The predicted protein contains a conserved proteolytic cleavage site (RXRR) and nine conserved cysteine residues at the C terminus. RT-PCR revealed that the unprocessed and prodomain myostatin mRNAs were predominantly present in muscle, with limited expression in other tissues. However, the mature myostatin mRNA was highly expressed in brain and muscle, intermediately expressed in the gills, intestine, heart, and kidney, and weakly expressed in the liver and spleen.