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Effects of Added Vegetable Oils on In vitro Formation of Fatty Acid Soaps and Fermentation Characteristics and NDF Disappearance Rate (식물성유 첨가가 In vitro 발효성상, NDF 소실율 및 지방산염 형성에 미치는 영향)

  • Kim, D.I.;Choi, J.R.;Lee, Y.H.;Lee, J.K.;Chung, T.Y.
    • Journal of Animal Science and Technology
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    • v.46 no.3
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    • pp.355-372
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    • 2004
  • In vitro experiments were conducted to determine the formation of fatty acid soaps (FAS) and neutral detergent fiber (NDF) disappearance rate. The substrates were a basal alfalfa hay containing 1) no oil, 2) 10% soybean oil, 3) 10% com oil, on a weight basis. All the substrates were incubated in triplicate for 0, 3, 6, 12, 24 and 48h in each experiment. After the incubation in the first experiment serum bottles (6oml) were analyzed for nonesterified, esterifed and fatty acid soaps contents. The serum bottles (120mI) from the second experiment were analyzed for pH, $NH_3-N$ and VFA concentration, and dry matter and NDF disappearance rate. pH decreased and the concentration of NH3-N increased significantly with longer incubation time (P<0.0001). The disappearance rates of dry matter and NDF significantly varied with feed, incubation time and oils (P<0.05). The molar concentration of total VFA increased and proportion of acetate significantly decreased with incubation time (P<0.0001), but the proportion of propionate significantly increased with longer incubation time (P<0.0001). Addition of oils to diet lowered the ratio of acetate:propionate (P<0.05). The esterified fatty acids (EFA) decreased with increasing incubation time (P<0.0001), and nonesterified fatty acids (NEFA) increased due to lipolysis of EFA, NEFA then reacted with cations to increase formation of FAS. The formation of FAS increased significantly at 48h of incubation time (P<0.0001). Especially, formation of stearic acid soaps was 27.5 and 32.5 folds with soybean oil and com oil supplements, respectively, by 48h of incubation time (P<0.0001). Alfalfa hay had higher cation contents, particularly Ca, which react with NEFA and FAS can be formed with longer incubation time. Saturated fatty acids had a higher proportion of FAS than did unsaturated fatty acids, suggesting that the former may react more extensively with cations. FAS contents increased with increasing chain length of the fatty acids. Since added vegetable oils fonned FAS, it might decrease negative effects on in vitro fermentation characteristics and NDF disappearance rate.

Response of the Growth Characteristics and Phytochemical Contents of Pepper (Capsicum annuum L.) Seedlings with Supplemental LED Light in Glass House (LED 보광처리가 고추(Capsicum annuum) 묘의 생장과 Phytochemical 함량에 미치는 영향)

  • Azad, Md. Obyedul Kalam;Chun, Ik-Jo;Jeong, Jeong-Hak;Kwon, Soon-Tae;Hwang, Jae-Moon
    • Journal of Bio-Environment Control
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    • v.20 no.3
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    • pp.182-188
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    • 2011
  • This research was conducted to evaluate the effect of supplemental light-emitting diode (LED) light on growth characteristics and phytochemical content of pepper (Capsicum annuum L.) seedling using LED blue (470 nm, B), red (660 nm, R), blue + red (BR), far red (740 nm, FR) and UV-B (300 nm) light treatment, and without artificial light. Photon flux of LED light was 49, 16, 40, 5.0 and $0.82{\mu}mol\;m^{-2}s^{-1}$ for B, R, BR, FR, and UV-B light, respectively, during experiment. Supplemental LED light duration was $16hr\;day^{-1}$ and UV-B light duration was 10 min. per day after sunset up to 15 days (12 days after germination) of plants age. In our research, growth characteristics and phytochemical content of pepper seedlings were greatly influenced by supplemental LED light compare to control treatment. Red light increased the number of leaves, number of nodes, leaf width and plant fresh weight by 34%, 27%, 50% and 40%, respectively. Blue light increased the leaf length by 13%, and stem length and length of inter node were increased by 17% and 34%, respectively under grown far red light. After 15 days of light treatments phytochemical concentrations of pepper plants were significantly changed. Blue light enhanced the total anthocyanin and chlorophyll concentration by 6 times and 2 times, respectively. Red light increased the total phenolic compound at least two folds meanwhile far red light reduced the ascorbic acid and antioxidant activity 31% and 66%, respectively compared to control treatment.

Low Temperature Inducible Acid Tolerance Response in virulent Salmonella enterica serovar Typhimurium (병원성 Salmonella enterica serovar Typhimurium의 저온 유도성 산 내성 반응)

  • Song, Sang-Sun;Lee, Sun;Lee, Mi-Kyoung;Lim, Sung-Young;Cho, Min-Ho;Park, Young-Keun;Park, Kyeong-Ryang;Lee, In-Soo
    • Korean Journal of Microbiology
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    • v.37 no.3
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    • pp.228-233
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    • 2001
  • The acid tolerance response (ATR) of log-phase Salmouella enterica seroyar Typhimurium is induced by acid adaptation below pH4.5 and will protect cells against more severe acid. Two distinctive ATR systems in thisorganism are a log-phase and stationary-phase ATR in which acid adaptations trigger the synthesis of acid shockproteins (ASPs). We found that log-phase ATR system was strongly affected by environmental factor, low tem-perature, $25^{\circ}C$. Exposure to low temperature and mild acid has been shown to increase acid survival dra-matically, and this survival rate was showed higher than $37^{\circ}C$. Especially unadapted cells at $25^{\circ}C$ presented tenthousand folds survival increasing when compared with cells at $37^{\circ}C$. The degree of acid tolerance of rpoSwhich is blown to be required for acid tolerance more increase than $37^{\circ}C$. Even though AIR pattern of rpoSbetween unadapted and adapted was showed similar at pH 3.1, rpoS-dependent ATR system also has beendetected in low temperature because rpoSAp prevents sustained acid survival at $25^{\circ}C$. Therefore the resultssuggest low temperature ATR system requires rpoS-dependent and -independent both. To investigate the basisfor low temperature related ATR system, gene that was participated for low temperature acid tolerance (lat) wasscreened in virulent S. enterica serovar Typhimurium UKl Using the technique of P22- MudJ (Km, lacZ)-directed lacZ operon fusion, LF452 latA‥‥MudJ was isolated. The latA‥‥MudJ of S. enterica Typhimurium pre-vented low temperature acid tolerance response. Therefore latA is considered one of the important genes for acidadaptation.

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Immuno-stimulating and Antitumor Effects of Crude Polysaccharides Extracted from Fruiting Body of Grifola frondosa (잎새버섯(Grifola frondosa)의 자실체에서 추출한 조다당류의 면역증강 및 항암효과)

  • Kim, Jeong-Hwa;Cha, Youn-Jeong;Shim, Mi-Ja;Lee, Min-Woong;Lee, Tae-Soo
    • The Korean Journal of Mycology
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    • v.39 no.1
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    • pp.68-77
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    • 2011
  • 80% methanol and 0.9% neutral saline soluble and hot water substances (hereinafter referred to Fr. NaCl, Fr. HW and Fr. MeOH, respectively) were extracted from fruiting bodies of Grifola frondosa. In vitro cytotoxicity tests, crude polysaccharides were not cytotoxic against cancer cell lines such as Sarcoma 180 and RAW 264.7 at the concentration of 10~2000 ${\mu}g/ml$, but crude polysaccharides from Fr. NaCl was slightly toxic to HT-29 and NIH3T3 at the concentration of 2000 ${\mu}g/ml$. Intraperitoneal injection with crude polysaccharides exhibited life prolongation effect of 25.0~52.9% in mice previously inoculated with Sarcoma 180. Fr. HW increased the numbers of spleen cells by 1.3 fold at the concentration of 200 ${\mu}g/ml$ compared with control. Fr. NaCl improved the immuno-stimulating activity of B lymphocyte by increasing the alkaline phosphatase activity by 1.5 fold compared with control at the concentration of 200 ${\mu}g/ml$. 10~14 ${\mu}M$ of nitric acid were generated when Fr. NaCl was added to RAW 264.7 at the concentration of 50~500 ${\mu}g/ml$, while the control group produced 4.3 ${\mu}M$ of nitric oxide. The Fr. NaCl, Fr. HW and Fr. MeOH increased the production of TNF-${\alpha}$, IL-$1{\beta}$, Il-2 and IL-6 by more than 1.4 times compared with the control group. The Fr. of MeOH increased the numbers of peritoneal exudate cells and circulating leukocytes by 3.0 and 2.0 folds compared with the control at the concentration of 50 ${\mu}g/ml$, respectively. Therefore, the crude polysaccharides extracted from fruiting bodies of Grifola frondosa could improve antitumor activity of mice.

Geological Structure around Andong Fault System, Pungcheon-myeon, Andong, Korea (안동시 풍천면 안동단층계 주변의 지질구조)

  • Kang, Ji-Hoon;Lee, Duck-Seon
    • The Journal of the Petrological Society of Korea
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    • v.17 no.2
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    • pp.83-94
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    • 2008
  • The Pungcheon-myeon, Andong, consists mainly of Precambrian metamorphic rocks, Jurassic igneous rocks, Cretaceous sedimentary rocks (Hasandong, Jinju and Iljik Formations) and Cretaceous igneous rocks (gabbroic rocks, dykes), in which several major faults are developed; Andong fault of ENE trend, which is the boundary fault of the Cretaceous Gyeongsang Basin and the Precambrian-Jurassic basement (Yeongnam Massif), Namhu fault parallel to it, Maebong fault of NNW direction, bow-shaped Gwangdeok fault of ENE direction which is convex toward SSE direction, and Hahoe fault of NNE direction. This paper is researched the geological structures around these major faults by means of the detailed geometric analysis on beddings, joints, faults and drag folds. As a result, a reverse slip faulting of top-to-the SSE movement accompanied with a regional drag folding is recognized from the arrangement of bedding poles measured around the Gwangdeok and Hahoe faults at its northeastern extension, and a zone of Gwangdeok drag fold of 150-300 m width, which is wider at the central and eastern parts of Gwangdeok fault and narrower at its western part and Hahoe fault, is also defined. It indicates that the Hahoe and Gwangdeok faults are a single fault and their movements are coeval unlike the results of earlier reasearchers. And, In this area are recognized two types of faults [(E)NE${\sim}$EW(fault I), WNW${\sim}$NNW (fault II), trending faults] and four types of joints [EW (I), (N)NW (II), NNE (III), NE (IV) trending joints]. These fractures were formed at least through four different events, named as Dn to Dn+3 phases. (1) Dn phase; the formation of joint (I) (Gwangdeok joint) and the intrusion of acidic dykes of EW trend under the compression of EW direction. (2) Dn+1 phase; the formations of joint (II) (Maebong joint), lens-shaped boudinage of acidic dykes, oblique-slip reverse fault (Fault I-Gwangdeok fault) under the compression of (N)NW direction, and the formation of regional zone of Gwangdeok drag fold accompanying the Gwangdeok faulting. (3) Dn+2 phase; those of joint (III), Fault II (Maebong fault) by dextral strike-slip movement of Maebong joint under the compression of NNE direction, and the extension cutting of Dn+1 structures due to the Maebong faulting. (4) Dn+3 phase; the jointing (IV) and the reactivation of Fault II as oblique-slip type with predominant dextral motion which took place under the compression of NE direction. It also suggests that the Maebong fault is not a tear fault deveolped during thrust tectonics of the Andong and Gwangdeok faults but is a post-fault during different tectonic event.

Human Resource Management Policy for University Faculty enhancing University-Industry Cooperation (산업현장친화형 대학교원 인사제도의 방향)

  • Jang, Seungkwon;Choi, Jong-In;Hong, Kilpyo
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.8 no.4
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    • pp.95-109
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    • 2013
  • The practices and processes of HRM (Human Resource Management) for university faculty in Korea depend heavily on assessment of research and teaching rather than the UIC (University-Industry Cooperation) performance. In this regard, HRM of Korean universities is said to be far distant from UIC. Although policy initiatives by the Korean government, notably the MoE (Ministry of Education) have implemented in most universities, the desirable level of UIC could not be achieved yet. Moreover, the very notion of 'university' in Korea is much more to do with 'pure' education and research institution than with 'applied' and 'vocational' purpose. Considering upon HRM practices and organizational culture, for enhancing UIC in Korea, the government's policy should be linked to alter deep-rooted university culture. So the aims of the research are to describe the current state of HRM in Korean and foreign universities; to find out the critical factors of UIC in Korean universities; to analyze the gaps between university research and industrial commercialization based on a conceptual framework, the 'valley of the death'; and to recommend HRM policies fostering UIC for the MoE. For achieving these objectives, we deploy multiple methodologies, namely, in-depth interview, literature survey, and statistical data analysis with regard to UIC. Analyzing the data we have collected, the present research sheds light on all aspects of HRM processes and UICs. And the main policy implication is restricted to the Korean universities, even if we have collected and analyzed foreign universities, notably universities in the USA. The research findings are mainly two folds. Firstly, the HRM practices among Korean universities are very similar due to the legally institutionalized framework and the government's regulations. Secondly, the difficulties of UIC can be explained by notion of the 'valley of death' ways in which both parties of university and industry are looking for different purposes and directions. In order to overcome the gap in the valley of death, the HRM policy is better to be considered as leverage. Finally, the policy recommendations are as follows. Firstly, various kinds of UIC programs are able to enhance the performances of not only UIC, but also education and research outcome. Secondly, fostering organizational climate and culture for UIC, employing various UIC programs, and hiring industry-experienced faculty are all very important for enhancing the high performance of university. We recommend the HRM policies fostering UIC by means of indirect way rather than funding directly for university. The HRM policy of indirect support is more likely to have long-term effectiveness while the government's direct intervention to UIC will have likely short-term effectiveness as the previous policy initiatives have shown. The MEST's policy means of indirect support might vary from financial incentives to the universities practicing HRM for UIC voluntarily, to information disclosure for UIC. The benefits of the present research can be found in suggesting HRM policy for UIC, highlighting the significance of industry-experienced faculty for UIC, and providing statistical analysis and evidences of UIC in Korean universities.

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Functional MR Imaging of Cerbral Motor Cortex: Comparison between Conventional Gradient Echo and EPI Techniques (뇌 운동피질의 기능적 영상: 고식적 Gradient Echo기법과 EPI기법간의 비교)

  • 송인찬
    • Investigative Magnetic Resonance Imaging
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    • v.1 no.1
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    • pp.109-113
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    • 1997
  • Purpose: To evaluate the differences of functional imaging patterns between conventional spoiled gradient echo (SPGR) and echo planar imaging (EPI) methods in cerebral motor cortex activation. Materials and Methods: Functional MR imaging of cerebral motor cortex activation was examined on a 1.5T MR unit with SPGR (TRfrE/flip angle=50ms/4Oms/$30^{\circ}$, FOV=300mm, matrix $size=256{\times}256$, slice thickness=5mm) and an interleaved single shot gradient echo EPI (TRfrE/flip angle = 3000ms/40ms/$90^{\circ}$, FOV=300mm, matrix $size=128{\times}128$, slice thickness=5mm) techniques in five male healthy volunteers. A total of 160 images in one slice and 960 images in 6 slices were obtained with SPGR and EPI, respectively. A right finger movement was accomplished with a paradigm of an 8 activation/ 8 rest periods. The cross-correlation was used for a statistical mapping algorithm. We evaluated any differences of the time series and the signal intensity changes between the rest and activation periods obtained with two techniques. Also, the locations and areas of the activation sites were compared between two techniques. Results: The activation sites in the motor cortex were accurately localized with both methods. In the signal intensity changes between the rest and activation periods at the activation regions, no significant differences were found between EPI and SPGR. Signal to noise ratio (SNR) of the time series data was higher in EPI than in SPGR by two folds. Also, larger pixels were distributed over small p-values at the activation sites in EPI. Conclusions: Good quality functional MR imaging of the cerebral motor cortex activation could be obtained with both SPGR and EPI. However, EPI is preferable because it provides more precise information on hemodynamics related to neural activities than SPGR due to high sensitivity.

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Geological Structures and Geochemical Uranium Anormal Zone Around the Shinbo Mine, Korea (신보광산 주변지역의 지질구조와 우라늄 지화학 이상대)

  • Kang, Ji-Hoon;Lee, Deok-Seon
    • Economic and Environmental Geology
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    • v.45 no.1
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    • pp.31-40
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    • 2012
  • This paper examined the characteristics of ductile and brittle structural elements with detailed mapping by lithofacies classification to clarify the relationship between the geological structure and the geochemical high-grade uranium anormal zone and to provide the basic information on the flow of groundwater in the eastern area of Shinbo mine, Jinan-gun, Jeollabuk-do, Korea. It indicates that this area is mainly composed of Precambrian quartzite, metapelite, metapsammite, which show a zonal distribution of mainly ENE-WSW trend, and age unknown pegmatite and Cretaceous porphyry which intrude them. But the Cretaceous Jinan Group which unconformably covers them, contrary to assumption, could not be observed. The main ductile deformation structures of Precambrian metasedimentary rocks were formed at least through three phases of deformation [ENE striking regional foliation (D1) -> ENE or EW striking crenulation foliation (D2) -> WNW or EW trending open, tight, kink folds (D3)]. The predominant orientation of S1 regional foliation strikes ENE and dips south, being similar to the zonal distribution of Precambrian metasedimentary rocks. Most predominant orientation of high-angled brittle fracture (dip angle ${\geq}45^{\circ}$) [ENE (frequency: 24.3%) > NS (23.9%) > (N)NW (18.8%) > WNW (16.9%) > NE (16.1%) fracture sets in descending frequency order], which is closely related to the flow of groundwater, strikes ENE and dips south. It also agrees with the zonal distribution of metasedimentary rocks and the predominant orientation of S1 regional foliation. The next one strikes NS and dips east or west. Considering the controlling factor of the geochemical uranium anormal zone in the Shinbo mine and its eastern areas from the above structural data. the uranium source rock in these areas might be pegmatite and the geochemical uranium anormal zone in the Sinbo mine area could be formed by an secondary enrichment through the flow of pegmatite aquifer's groundwater into the Sinbo mine area like the previous research's result.

Effect of Cadmium-contaminated Brown Rice Diet on Accumulation of Heavy Metal in Rats (카드뮴 오염 현미 섭취에 의한 랫드의 체내 중금속 축적)

  • Kim, Jae-Young;Im, Hyo-Bin;Kim, Seong-Jo;Baek, Seung-Hwa
    • Journal of Food Hygiene and Safety
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    • v.27 no.2
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    • pp.133-140
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    • 2012
  • Movement and accumulation of cadmium in male Sprague-Dawley rats, fed with brown rice from nearby Janghang smeltery area were investigated. The rat fed with five different cadmium level diets made with Cd-polluted during 12 weeks. The brown rice-polluted with 0.87 ppm Cd (PBR) was sampled from products in the Janghang smeltery area. Diets of brown rice group were brown rice (BR, 0.002 ppm Cd), each 50% of BR and PBR (BR+PBR 50%, 0.44 ppm Cd) and PBR (PBR 100%, 0.87 ppm Cd). To compare with BR+PBR 50%, the another group diet composed the feed (FE, 0.002 Cd ppm) and each 50% of FE and PBR (FE+PBR 50%, 0.44 ppm Cd). Accumulation of Cd, Zn and Cu in blood, liver and kidney rats was measured by GF-AAS. The weight gain in BR groups and FE groups were different 0.22-0.26 and 1.08-1.26 g/day, respectively. Daily intake cadmium was 10.77 and 22.36 ${\mu}g/rat$ in BR+PBR 50% and PBR 100%, and 8.83 ${\mu}g/rat$ in FE+PBR 50%. Cadmium contents in diets were higher, and total intake of the heavy metals was more increased on the whole. Weights of liver and kidney in FE+PBR 50% group was 2.64 and 2.27 folds higher than those in BR+PBR 50% group. Cadmium contents in blood were increased with intake of BR diet, but Zn and Cu were decreased with them. In the diet groups with the same Cd concentration, Cd content of FE+PBR 50% was higher 1.27 times than that of BR+PBR 50%. In the diet group of BR, BR+PBR 50%, and PBR 100%, the increase of Cd concentration was significantly different to the increase of Cd content in the livers. In the same condition of Cd concentration, Cd contents were higher in the BR+PBR 50% group. In the diet groups of BR, BR+PBR 50%, and PBR 100%, the increase of Cd content in the kidneys led to the increase of Zn and Cu contents. In the same condition of Cd concentration, the diet group with the addition of BR was shown to be 3.11 times higher than with the addition of FE. In view of the results so far achieved, It was closely related with Cd, Zn, and Cu content.

Microbiological Study using Monitoring of Microorganism in Salt-Fermented Fishery Products (젓갈류에서의 위생지표 미생물 및 식중독균 모니터링을 통한 미생물학적 연구)

  • Lee, Sun-Mi;Lim, Jong-Mi;Kim, Ki-Hyun;Cho, Soo-Yeol;Park, Kun-Sang;Sin, Yeong-Min;Cheung, Chi-Yeun;Cho, Joon-Il;You, Hyun-Jeong;Kim, Kyu-Heon;Cho, Dae-Hyun;Lim, Chul-Ju;Kim, Ok-Hee
    • Journal of Food Hygiene and Safety
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    • v.23 no.3
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    • pp.198-205
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    • 2008
  • In this study, microbial investigation is accomplished for 554 Jeot-kal samples(102 of Jeot-kal, 448 of Seasoned Jeot-kal and 4 of Sik-khe, respectively) that corresponds with Coliform-bacteria, Escherichia coli, Aerobic live bacteria as hygienic indicator microorganisms, and Staphylococcus aureus, Vibrio parahaemolyticus as Food-borne pathogenic microorganisms, Based on the methods in Korea Food Code, reliable data are obtained as follows; in 31.9% rate of the samples, Coliform bacteria are verified in the extent of $0{\sim}20,000$ CFU/g as 2.3 logCFU/g. Especially, Seasoned Jeot-kal(37.7%,2.3 logCFU/g) are detected to 6 and 2 folds higher than those of Jeot-kal, 5.9% and 1.4 logCFU/g. Likewise, Escherichia coli is detected from 9 samples only in Seasoned Jeot-kal, that includes seasoned squid, seasoned octopus, seasoned roe of pollack, seasoned large-eyed herring and seasoned hairtail. Aerobic live bacteria are also detected in the range of $0{\sim}8.9{\times}10^8CFU/g$. Against salinity, E. coli are detected in samples only less than 10% salinity. Concomitantly, aerobic live bacteria count is decreased to $5.5{\sim}3.6$ log CFU/g upon the salinity is increased up to 25%. However, S. aureus and V. parahaemolyticus are not detected in 554 samples, presumptively referring Jeot-kal products are somehow free from such food-borne pathogens. As the results above, we deliberately consider that the sanitary control in Jeot-kal, which be necessarily fermented- as well as non-microbially inactivated should be ensured in near future and also suggest an effectual microbial standard corresponding to the Negativity in E. coli for Jeot-kal products.