• Title/Summary/Keyword: folding system

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Interaction of a 22 kDa Peptidyl Prolyl cis/trans Isomerase with the Heat Shock Protein DnaK in Vibrio anguillarum

  • Kang, Dong Seop;Moon, Soo Young;Cho, Hwa Jin;Lee, Jong Min;Kong, In-Soo
    • Journal of Microbiology and Biotechnology
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    • v.27 no.3
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    • pp.644-647
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    • 2017
  • Peptidyl prolyl cis/trans isomerases (PPIases) catalyze the cis/trans isomerization of peptidyl-prolyl peptide bonds preceding prolines. We investigated the protein-protein interaction between a 22 kDa PPIase (VaFKBP22, an FK506-binding protein) and the molecular chaperone DnaK derived from Vibrio anguillarum O1 (VaDnaK) using GST pull-down assays and a bacterial two-hybrid system for in vivo and in vitro studies, respectively. Furthermore, we analyzed the three-dimensional structure of the protein-protein interaction. Based on our results, VaFKBP22 appears to act as a cochaperone of VaDnaK, and contributes to protein folding and stabilization via its peptidyl-prolyl cis/trans isomerization activity.

Effects of Co-Expression of Liver X Receptor β-Ligand Binding Domain with its Partner, Retinoid X Receptor α-Ligand Binding Domain, on their Solubility and Biological Activity in Escherichia coli

  • Kang, Hyun
    • Journal of Microbiology and Biotechnology
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    • v.25 no.2
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    • pp.247-254
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    • 2015
  • In this presentation, I describe the expression and purification of the recombinant liver X receptor β-ligand binding domain proteins in E. coli using a commercially available double cistronic vector, pACYCDuet-1, to express the receptor heterodimer in a single cell as the soluble form. I describe here the expression and characterization of a biologically active heterodimer composed of the liver X receptor β-ligand binding domain and retinoid X receptor α-ligand binding domain. Although many of these proteins were previously seen to be produced in E. coli as insoluble aggregates or "inclusion bodies", I show here that as a form of heterodimer they can be made in soluble forms that are biologically active. This suggests that co-expression of the liver X receptor β-ligand binding domain with its binding partner improves the solubility of the complex and probably assists in their correct folding, thereby functioning as a type of molecular chaperone.

A Study on Code Assignment to Microinstructions (Microinstruction의 부호 할당에 관한 연구)

  • 오창준;이철동;유영욱
    • Journal of the Korean Institute of Telematics and Electronics
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    • v.25 no.1
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    • pp.108-114
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    • 1988
  • We have developed a program that minimizes the chip area with effective code assignment of microinstructions when the control part of VLSI system is implemented in the PLA. While conventional algorithms are composed of one pass, the algorithm proposed in this paper is composed of two passes and it consists of three principal procedures: a procedure for the determination of the code assignment order, a procedure for the code assignment and a procedure for the reduction of the code length. especially, we have proposed a new method of folding back for the reduction of the code length. We have obtained the result of 4 bit code length and 9 product terms when 11 micro-instructions and 6 microoperations were given as a inputs. Therefore, we have certified that it could be used efficiently for the control part of the processors.

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The Design of a "Motorized Wheelchair(INMEL-5)" for Loading in the Car (차량 탑재용 전동휠체어(INMEL-5)의 설계(1))

  • 정동명;홍승홍
    • Journal of Biomedical Engineering Research
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    • v.11 no.1
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    • pp.105-112
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    • 1990
  • The main concepts in the design and construction of an motorized wheelchair for loading in the tar which is intended to drive a disabled to long distance. The wheelchair is basically a powered and folding, so it is designed to motorized manual wheel- chair by modularity method. The wheelchair hrs been installed with a power motor module, battery module, and drive & control module. The goal of this project is to develop a wheelchair that has multifunction and operated disabled who has variety impairment. So we ave currently working. Experimental results of the system approach our purpose, which is improved maneuverability, safety and can be loaded.

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Sine Scan Inhomogeneity Field Mapping using Multiple Echo (다중 Echo를 이용한 Single Scan Inhomogeneity Field Mapping)

  • Kim, C.Y.;Han, S.Y.;Jung, K.W.;Oh, C.H.;Yi, Y.;Ahn, C.B.
    • Proceedings of the KOSOMBE Conference
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    • v.1998 no.11
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    • pp.307-308
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    • 1998
  • In this paper, we describe a field measurement technique using multiple gradient echo method. For this purpose, 3 differential phase map is used between two images obtained in a single scan. The phase map is further processed to unfold phase folding (2n $\pi$). The inhomogeneity map is used to correct image distortion along readout gradient direction. Some experimental results obtained at 1.0 Tesla whole body MR system are shown with the proposed technique.

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Development of the Foldable Manual/Power Hybrid Wheelchair ($\cdot$전동 복합기능의 접이식 휠체어 개발)

  • Choi Young Chul;Rhee Kun Min;Choi Hwa Soon;Seo Young Taek
    • Journal of the Korean Society for Precision Engineering
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    • v.23 no.2 s.179
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    • pp.172-180
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    • 2006
  • Although there existed many types of manual/power hybrid wheelchairs, their use was not widespread because of their inconvenience in converting drive system and in folding frames. To carry a wheelchair in the car or to convert driving methods, some hard work of disassembling or exchanging wheels was required for most of currently available hybrid wheelchairs. In this study, the standard foldable manual wheelchair was reformed to a power wheelchair by installing the newly developed Axial Flux Permanent Magnet(AFPM) type of brush less direct current(BLDC) motor on each rear hub of wheelchair. This wheelchair could be driven by manual or electric power without exchanging. wheels, thus no additional work was needed for carriage or for power conversion. The developed wheelchair was evaluated for durability, stability, maneuverability, cost, and reliability in accordance with the Korean standards. The results indicated that the developed hybrid wheelchair was good enough for commercialization comparing to other imported wheelchairs.

Automatic Layout of High Density PLA (고밀도 PLA의 자동 Layout System의 구성)

  • 이제현;경종민
    • Journal of the Korean Institute of Telematics and Electronics
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    • v.22 no.6
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    • pp.13-18
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    • 1985
  • A set of utility programs for automatic generation, minimization and verification of high density PLA layout was developed, which includes equation-to-truth table translator, logic minimizer, PLA product term sorter, file generator for plotting stick diagram, dynamic CMOS PLA layout generator and bipartite row folded CMOS PLA layout generator. Size reduction is performed mainly by logic minimizer and bipartite row folder, and the maximal delay is reduced by sorter. The fOe for automatically generated layout is stored in CIF. Each program was written in Clanguage, and was run on VAX-11/750 (UNIX).

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Automated Wireless Recharging for Small UAVs

  • Jung, Sunghun;Ariyur, Kartik B.
    • International Journal of Aeronautical and Space Sciences
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    • v.18 no.3
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    • pp.588-600
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    • 2017
  • We develop a wireless, contact free power transfer mechanism that is safer than the direct metallic contact and robust to imperfect alignment on landing at the base station. A magnetic field is created using inductors on both the transmitting and receiving sides. We use the inductive wireless recharging to increase autonomy and decrease the sensor interference by reducing the inductor loop size. By locating four independent small receiver loops and corresponding four circuits around the quadrotor UAV, we can increase safety from circuit malfunctions in comparison to the use of just one loop. On the base station, four folding robotic bars are used to realign the receiver loops over the transmitter loops. After adequate recharging as measured by battery voltages or power consumption at the bae station, the UAV sends a signal to the base station to open the robotic bars and takes off once freed from the robotic bars.

Hybrid Dynamic Branch Prediction to Reduce Destructive Aliasing (슈퍼스칼라 프로세서를 위한 고성능 하이브리드 동적 분기 예측)

  • Park, Jongsu
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.23 no.12
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    • pp.1734-1737
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    • 2019
  • This paper presents a prediction structure with a Hybrid Dynamic Branch Prediction (HDBP) scheme which decreases the number of stalls. In the application, a branch history register is dynamically adjusted to produce more unique index values of pattern history table (PHT). The number of stalls is also reduced by using the modified gshare predictor with a long history register folding scheme. The aliasing rate decreased to 44.1% and the miss prediction rate decreased to 19.06% on average compared with the gshare branch predictor, one of the most popular two-level branch predictors. Moreover, Compared with the gshare, an average improvement of 1.28% instructions per cycle (IPC) was achieved. Thus, with regard to the accuracy of branch prediction, the HDBP is remarkably useful in boosting the overall performance of the superscalar processor.

An improved method to determine hydroxyproline in an immortalized rat liver stellate cell line (HSC-T6)

  • Lee, Eun-Su;Kwak, Wie-Jong;Lee, Eung-Seok;Soh, Yun-Jo
    • Proceedings of the PSK Conference
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    • 2003.04a
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    • pp.154.1-154.1
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    • 2003
  • Hydroxyproline (HYP) is a post-translational product of proline hydroxylation catalyzed by an enzyme prolyl 4-hydroxylase which plays a crucial role in the synthesis of all collagens, because the 4-hydroxyproline residues are essential for the folding of the newly synthesized collagen polypeptide chains into triple-heical molecules. Considering the role of collagen and its significance in many clinically important diseases such as liver cirrhosis, a great deal of attention has been directed toward the development of an assay at cell-based system. (omitted)

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