• Biotechnology and Bioprocess Engineering:BBE
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• v.8 no.3
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• pp.167-172
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• 2003

Foam fractionation can be used to enrich a hydrophobic protein such as bromelain from an aerated dilute protein solution because the protein foams. On the other hand, a protein such as invertase, which is hydrophilic, is not likely to foam under similar aerated conditions. While a foam fractionation process may not be appropriate for recovering a hydrophilic protein alone, it is of interest to see how that non-foaming protein affects the foaming protein when the two are together in a mixture. The bromelain enrichment, activity and mass recovery were observed as a function of the solution pH in order to explore how invertase can affect the recovery of bromelain in a foam fractionation process.

• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.1
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• pp.26-30
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• 2002

A droplet fractionation method was previously developed to concentrate a dilute nonfoaming protein solution. In that earlier study with invertase, it was demonstrated that droplets created by ultrasonic energy waves could be enriched up to 8 times that of the initial dilute invertase solution. In this study, a mixture of bromelain (a foaming protein) and invertase (a nonfoaming protein) is investigated as a preliminary step to determine if droplet fractionation can also be used to separate a non-foaming protein from foaming proteins. The foaming mixture containing bromelain is first removed by bubbling the binary mixture with air. After the foam is removed, the protein rich air-water interfacial layer is skimmed off (prior to droplet fractionation) so as not to interfere with the subsequent droplet production from the remaining bulk liquid, rich in non-foaming protein. Finally, sonic energy waves are then applied to this residual bulk liquid to recover droplets containing the non-foaming protein, presumed to be invertase. The primary control variable used in this droplet fractionation process is the pH, which ranged for separate experiments between 2 and 9. It was observed that the maximum overall protein partition coefficients of 5 and 4 were achieved at pH 2 and 4, respectively, for the initial foaming experiment followed by the post foaming droplet fractionation experiment.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.515-518
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• 2002

The method of foam fractionation can be applied to enrich proteins from a dilute protein solution if the proteins are hydrophobic and foam. If a protein, such as invertase, is hydrophilic, a dilute solution containing this protein may not foam. In that case, a batch foam fractionation process may not be appropriate for recovering a concentrated solution of that protein. In this paper, various concentrations of invertase were added to a dilute solution containing bromelain (a hydrophobic protein), in order to determine how the presence of a hydrophilic protein can affect the recovery of the desired hydrophobic protein. The effect of invertase on bromelain recovery was studied here at an initial bulk solution pH of 5 and an air superficial velocity of 4.6 cm/s.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2003.05a
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• pp.221-222
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• 2003

Typically, closed production system units are subject to an accumulation of fine suspended solids and dissolved organics (Weeks et at., 1992). Foam fractionation process is believed to be most effective in marine application for solids removal. In present experiment, the performance of foam fractionator for removal of solids, protein, and other dissolved materials was evaluated at different foam overflow heights and air flow rates in a pilot-scale recirculating aquaculture system for culture of Korean rockfish. (omitted)

• Fisheries and Aquatic Sciences
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• v.6 no.4
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• pp.187-193
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• 2003

The performance of a foam fractionator to remove TAN, $NO_2,\;NO_3$, TSS, protein, and $PO_4-P$ at different superficial air velocities and foam overflow heights was evaluated in a lab-scale seawater recirculating system for culture of Korean rockfish (Sebastes schlegeli). The foam overflow rates increased with the increase of superficial air velocities, but decreased with the increase of foam overflow heights. Concentrations of all the water quality variables in the foam condensates increased with the increase of foam overflow height, but decreased with the increase of superficial air velocities. TSS, protein, and phosphate enrichment factors were within the range of 6.4-39.4, 1.6-7.3 and 1.2-3.9, respectively. Low values of TAN, $NO_2,\;and\;NO_3$ enrichment factors were obtained and they indicate that foam fractionation is rot an effective way to remove dissolved inorganic nitrogen. The calculated maximum daily removal values for TSS and protein were 10.9 and 1.4g, respectively.

• Journal of Aquaculture
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• v.16 no.4
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• pp.216-222
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• 2003

In a simulated seawater aquaculture system, effects of different operating factors like the superficial air velocity (SAY), hydraulic residence time (HRT), protein concentration and foam overflow height on the removal of total suspended solids (TSS) by a foam fractionator, with 20 cm diameter and 120 cm height, were investigated. This experiment was performed on batch and consecutive modes for different combinations of the tested factors, using synthetic wastewater. In 5 consecutive trials, TSS concentration in culture tank water decreased faster, when the foam fractionator was operated at higher SAV and lower HRT. In batch trials, with increasing SAV, TSS removal rate increased, but decreased with increasing HRT. Higher protein concentration in the bulk solution resulted in higher TSS removal rate. TSS concentration in the collected foam condensates increased but the foam overflow rate decreased with increasing foam overflow height. Foam fractionation was effective for removing TSS in seawater aquaculture systems and its performance largely depended on the operating parameters, especially superficial air velocity.

• Ocean and Polar Research
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• v.25 no.3
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• pp.269-275
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• 2003

Effects of different operating factors including superficial air velocity (SAV), hydraulic residence time (HRT), protein concentration, and foam overflow height on protein removal by a foam fractionator in simulated seawater aquaculture system were investigated. This experiment was conducted on batch and consecutive modes at different combinations of the affecting factors. The foam fractionator had a diameter of 20cm and a height of 120cm and the experiment was conducted with synthetic wastewater. In 5 consecutive trials, protein concentrations in culture tank water decreased faster when the foam fractionator was operated at higher SAVs and lower HRTs. In batch trials, protein removal rates increased with an increase in SAV but decreased with an increase in URT. Higher protein concentrations in the bulk solution resulted in higher protein removal rates. Protein concentrations in the collected foam condensates increased but the foam overflow rates decreased with the increase of foam overflow heights. The results of this experiment indicate that foam fractionation would be an effective way for protein removal in seawater aquaculture systems and the performance of the foam fractionator depends largely on the operating parameters, especially SAV.

• Journal of the Korean Applied Science and Technology
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• v.38 no.5
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• pp.1219-1228
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• 2021

Rhamnolipids are recognized as eco-friendly biosurfactants and produced by the bio-process employing bacteria. Pseudomons aeruginosa is well-known to produce rhamnolipids in high yield during fermentation process. Rhamnose and 𝛽-hydroxylated fatty acid are main chemicals for rhamnolipids, which are produced in the form of congener mixtures. In this paper, the synthetic mechanism of rhamnolipids within bacteria cells was presented in part and foam control technologies were qualitatively described. Foam control during fermentation process was important to regulate a continuous process. During last decade, the technologies are developed enough to challenge to a commercial-scale production. In particular, rhamnolipids will be more valuable if these can be applicable to value-added chemicals, such as medicines.

• Korean Journal of Food Science and Technology
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• v.19 no.3
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• pp.225-230
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• 1987

The foam separation of bovine serum proteins was investigated and the protein fractionation by foam separation was analyzed by PAG electrophoresis. The protein concentration for the surface excess formation of bovine serum was in the range of $20-800\;{\mu}g/ml$. At pH 5, the foamate volume was maximum, but the enrichment ratio minimum. As the temperature was elevated, the foamate volume decreased and the enrichment ratio increase. As the gas flow rate increased from 25 to 100 ml/min, the foamate volume decreased and the enrichment ratio increased. The enrichment ration became maximum when the added ionic strength of serum solution was in the range of 1-3 by the addition of different types of salts, and this was related to the reduction of surface tension of the solution. In general, BSA, ${\alpha}_1$, and ${\alpha}_2-globulins$, which have relatively small molecular weight and high hydrophobicity, moved easily to the foam, and the separation of protein fractions in the serum varied with the changes in pH, temperature, gas flow rate and ionic strength of the solution.

• Nuclear Engineering and Technology
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• v.15 no.2
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• pp.149-153
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• 1983

The removal of zinc (II) from aqueous solutions has been studied by the adsorptive bubble separation processes such as solvent sublation and foam fractionation processes using capric acid as collector. The efficiency of the remoal process has been studied by changing the ionic strength and pH and the bubble separation method has been compared with the solvent extraction process using the capric acid dissolved in benzene as extractant.