• BMB Reports
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• v.43 no.12
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• pp.813-817
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• 2010

Plants undergo cell division throughout their life in order to maintain their growth. It is well known that root and shoot tip of plants possess meristems, which contain quiescent cells. Fluridone (1-methyl-3-phenyl-5-(3-trifluromethyl (phenyl))-4-(1H)-pyridinone) is an established inhibitor of both ABA and carotenoid biosynthesis. However, the other functions of fluridone remain undiscovered. In this report, we provide experimental evidence that fluridone plays a role in the division of the quiescent centre of the Arabidopsis root meristem. This study examined the effects of exogenous fluridone and ABA on the development of the stem cell niche in Arabidopsis root. We show that fluridone promoted the division of stem cells in the quiescent centre, whereas exogenous ABA suppressed quiescent centre division. Furthermore, we established a novel regulatory function for fluridone by demonstrating that it plays an important role in postembryonic development.

• Korean Journal of Weed Science
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• v.2 no.2
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• pp.169-174
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• 1982

The distribution of major aquatic weeds in irrigation and drainage canals along Dongjin river and the degree of infestation of aquatic weeds were investigated throughout Korea, and chemical control of aquatic weeds was also studied. The major aquatic weed species in irrigation and drainage canals along Dongjin river were Leersia japonica, Ceratophyllum demersum, Zijania latifolia, Nuphar japonicum, Phragmites communis, Vallisneria asiatica, Trapa natans, Myriophyllum verticillatum, and Potamogeton crispus. Zijania latifolia, Phragmites communis, and Leersia japonica were troublesome weeds among emerged weeds throughout Korea. Caratophyllum demersum was most serious weed and Myriophyllum verticillatum, Potamogeton crispus, Vallisneria asiatica, and Potamogeton oxyphyllus were also heavily infested among submerged weeds. Leersia japonica was controlled by paraquat at 73.5g/10a glyphosate at 91.5g/10a, and fluridone at 74.7g/10a, Zijania latifolia by paraquat at 220.5g/10a, glyphosate at 366.0g/10a, and fluridone at 74.7g/10a, and Ceratophyllum demersum and Potamogeton crispus by 2,4,5-TP at 540g/10a and fluridone at 1008/10a.

• Plant Breeding and Biotechnology
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• v.5 no.3
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• pp.204-212
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• 2017

There is a growing preference for using doubled haploids (DHs) in maize breeding programs because they reduce the time required to generate and evaluate new lines to 2 years or less. However, there is an urgent need for efficient techniques that accurately discriminate between haploid and diploid maize kernels. Here, we investigate the effects of several hormones and chemicals on the germination of haploid and diploid maize kernels, including auxin, cytokinin, ethylene, abscisic acid (ABA) biosynthesis inhibitor (fluridone), ABA catabolism inhibitor (diniconazole), methyl jasmonate (MeJA), and NaCl. Ethylene effectively stimulated the germination of both haploid and diploid maize kernels. The ABA biosynthesis inhibitor fluridone, the ABA catabolism inhibitor diniconazole, and MeJA selectively stimulated the germination of haploid maize kernels. By contrast, gibberellin, 1-naphthaleneacetic acid (NAA), kinetin, and NaCl inhibited the germination of both haploid and diploid maize kernels. These results indicate that the germination of haploid maize kernels is selectively stimulated by fluridone and diniconazole, and suggest that ABA-mediated germination of haploid maize kernels differs from that of diploid maize kernels and other plant seeds.

• Journal of Bio-Environment Control
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• v.25 no.4
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• pp.240-248
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• 2016

The objective of this study was to determine the effect of exogenous abscisic acid (ABA) on the red coloration and endogenous ABA contents of apple fruit skins. ABA and fluridone (an ABA synthetic inhibitor, FD) was sprayed on 'Hongro' apple fruit skins at 107 days after full bloom (DAFB). Visual coloration and hunter's color values were not affected by the ABA and FD treatments. Anthocyanin contents in fruit skins increased similarly to hunter $a^*$ values of fruit skins, but ABA and FD did not affect its accumulations. Liquid chromatography analysis revealed that endogenous ABA contents in control fruit increased at first and then decreased from 12 hours after the treatment. ABA treatment increased ABA contents in fruit skins from 2 hour after the treatment and it lasted until the end of the treatments. FD decreased ABA contents in fruit skins from 6 hours after the treatment. ABA treatment increased MdNCED2 (an ABA biosynthetic gene), MdACO1 (an ethylene biosynthetic gene), and MdCHS and MdDFR expressions. However, MdUFGT expressions were not affected by ABA treatment.

• Korean Journal of Plant Tissue Culture
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• v.28 no.6
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• pp.317-321
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• 2001

This study was carried out with Lilium oriental hybrid cv. Casa Blanca to observe the effect of in vitro culture conditions on ex vitro sprouting of bulblets. Low temperature (15$^{\circ}C$) inhibited the growth of in vitro bulblets while high temperature ($25^{\circ}C$) enhanced the growth. Bulblets cultured at 15$^{\circ}C$ did not show dormacy while those cultured at 2$0^{\circ}C$ ,$25^{\circ}C$ had a longer dormancy period. High sucrose concentration (9%) induced longer dormancy. Dormancy period was also prolonged in bulblets cultured in vitro at high temperature ($25^{\circ}C$). Dormancy period was more affected by in vitro culture temperature rather than sucrose concentration. Physiological dormancy was released more rapidly when bulblets were cultured at $25^{\circ}C$ for 6 weeks and further transferred at 15$^{\circ}C$ and cultured for another 12 weeks. Treatment of ABA induced the dormancy in Lilium bulblets but when bulblets were subjected to chilling treatment (4$^{\circ}C$ for 8 weeks) nearly 100% sprouting were observed. The medium containing 1.0 mg/L BA or 1.0 mg/L fluridone was also effective to produce non-dormant bulblets.

• Korean Journal of Weed Science
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• v.16 no.4
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• pp.309-316
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• 1996

In this study, germination characteristics and herbicidal response of green kyllinga(Kyllinga brevifolia var. leiolepsis H.) were investigated. The storage method desirable for a rapid dormancy release was to keep the seed under low temp. and wetting condition for one to two months, or high temp($40^{\circ}C$) and drying condition for three months. The dormancy of rhizome was hardly observed. The optimum temperature for germination of seed and rhizome was around $30^{\circ}C$ and 16-$20^{\circ}C$, repectively. The germination of dormancy-breaked seed was completely dependent on light. Shoot emergence ratio(%) was decreased with increase of planting depth ; for example, only 18% of rhizome segments planted in the depth of 4cm under soil surface emerged above soil surface. Flooding at earlier growth stage resulted in significant decrease in shoot emergence as well as in dry weight. The germinablity of rhizome was almost lost as a decreased in fresh weight reached to 50%. Usually, green kyllinga was sensitive to herbicides such as bentazone, bensulfuron and benfuresate etc. which were known to be effective in Cyperaceae weeds, indicating that green kyllinga can be used as a representative plant in the screening of herbicides for Cyperus weeds.

• Korean Journal of Plant Tissue Culture
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• v.26 no.3
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• pp.183-187
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• 1999

To establish an efficient screening system for new herbicides using plant cultured cells, responses of tobacco photomixotrophic cultured (PH) cells to various herbicides with different modes of action were surveyed by measuring the cell growth and ion conductivity in medium. The cells were cultured in Murashige and Skoog (MS) medium containing 0.7mg/L 2,4-D, 0.3mg/L kinetin and 30 g/L sucrose at $25^{\circ}C$ in the light (100 rpm). Chemicals were treated to suspension cultures of tobacco PH cells at the time of subculture. The cell growth and ion conductivity in the medium were investigated on 12 days after chemical treatment. The ion conductivity assay gave well correlated results to the cell growth inhibition data. The responses of tobacco PM cells were dependent on the modes of action of chemicals tested. Atrazine, an inhibitor of photosynthetic electron transport (PET), strongly inhibited both the cell membrane and cell growth ($IC_{50}$/, about 1 $\mu$M). Butachlor (an inhibitor of cell division), glufosinate (an inhibitor of amino acid biosynthesis), and fluridone (an inhibitor of carotenoid biosynthesis) showed a dose-dependent inhibition. However, Quinclorac, a herbicide with an auxin activity, did not affect the cell growth and ion leakage. These results suggested that tobacco PM cells is suitable materials for the simple screening of new herbicides such as PET, amino acid biosynthesis, ceil division inhibitors by measuring the cell growth and ion conductivity.

• Journal of Plant Biotechnology
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• v.32 no.2
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• pp.97-103
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• 2005

Antioxidative responses of transgenic tobacco plants expressing both superoxide dismutase (SOD) and ascorbate peroxidase (APX) in chloroplasts was investigated with several herbicides. In greenhouse test, tolerance of SOD/APX-overexpressed tobacco (CA) to photosystem (PS) I inhibitor paraquat was increased by about 40%. However, any response differences between CA and wild type (WT) tobacco was not observed in a treatment with PS II inhibitors (bromoxynil, diuron and bromacil), chlorophyll biosynthesis inhibitor(oxyfluorfen), carotenoid biosynthesis inhibitor (fluridone) and 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase inhibitor (glyphosate). This tendency was also similar in the growth chamber test of low light intensity, using paraquat and diuron. That is, increased antioxidant activity of CA was shown only in paraquat treatment. When paraquat was foliar-treated to 6 to 9-leaf stage plant, the third to fourth placed leaf from shoot tip showed relatively higher antioxidant activity. Ascorbate supplemented to paraquat solution alleviated the phytotoxicity with a similar range in both CA and WT. In conclusion, CA specifically responded to oxidative stress induced by paraquat among tested herbicides in a whole plant assay.

• Journal of Plant Biotechnology
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• v.30 no.2
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• pp.207-214
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• 2003

This study was carried out to establish a novel bioassay system for screening of compounds affecting aromatic amino acid or anthocyanin biosynthesis through investigating a degree of sucrose-induced anthocyanin formation such as size of plant material, buffer conditions, light intensity and irradiated duration, incubation temp., etc were determined and standard procedure (suitable experimental condition) was set up as follows. The second leaf blade of white corn seeding induced by fluridone treatment were segmented into a size of 5${\times}$5 min. The segments were floated on the solution of 1% sucrose in 1.0mM MES buffer (pH6.0∼6.5) and incubated at 26$^{\circ}C$ for 2days under the continuous light condition(70∼100$\mu$mol m$^{-2}$ s$^{-1}$ ). Anthocyanin in the purpled tissues was extracted with methanol containing 1% HCl and the optical density of the clear supematants was determined at 528mm. Influences of some chemicals were tested using this system. Glyphosate, 5-enolpyruvylshikimate 3-phosphate synthase inhibitor, showed most sensitive response with I$_{50}$ value at 3.3$\mu$M. Dicyclohexylcarbodiimide(DCCD) and parachloromercuribenzenesulfonic acid(PCMBS) had a relatively strong ingibition with I50 value at 7.1$\mu$M and 10.2$\mu$M, respectively. These results show that sucrose-induced anthocyanin formation in white com leaf segment provide a very simple and rapid system for searching new compounds affecting aromatic amino acid or anteocyanin biosynthesis by screening at less than 10$\mu$M.