• ALGAE
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• 제20권2호
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• pp.113-120
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• 2005

The freshwater blooms mainly blue-green algal blooms occur frequently in the lower Naktong River in summer, which provoke many socio-economical problems; therefore, the early detection of bloom events are demanding through the quantitative and qualitative analyses of blue green algal species. The in vivo fluorescence properties of cultured strains of Microcystis aeruginosa, M. viridis, M. wesenbergii, M. ichthyoblabe, Anabaena cylindrica, A. flos-aquae, and Synedra sp. were investigated. Wild phytoplankton communities of the lower Naktong River were also monitored at four stations in terms of their standing stocks, biomass and fluorescence properties compared with its absorption spectram. The 77K fluorescence emission spectra of each cultured strains normalized at 620 nm was very specific and enabled to detect of blue green algal biomass qualitatively and quantitatively. The relative chlorophyll a concentration determined by chlorophyll fluorescence analysis method showed significant relationship with chlorophyll a concentration determined by solvent extraction method ($R^2$ = 0.906), and the blue-green algal cell number determined by microscopic observation ($R^2$ = 0.588), which gives insight into applications to early detection of blue green algal bloom.

• KSBB Journal
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• 제16권6호
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• pp.609-613
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• 2001

형광을 이용하여 중금속 이온의 농도를 측정할 수 있는 신규 단백질 바이오센서를 제작하였다. 바이오센서의 transducer로 카제인과 알부민을 사용하였을 때 $10^{-3}$mM-1 mM 범위에서 중금속 농도 정량에 우수성을 보였다. 또한 두 가지 중금속 혼합물에서 excitation과 emission파장에서 형광도를 측정하여 각 중금속 이온 농도를 선택적으로 정량하는 방법을 제시하였고, Co$^{2+}$, Fe$^{3+}$ 이온 혼합물을 대상으로 본 논문에서 제시한 방법의 유효성을 입증하였다.

• 한국식품과학회지
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• 제11권3호
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• pp.166-170
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• 1979

UVSL-25 mineral light를 조사(照射)한 경우에 발생하는 식품(食品) 보존료(保存料) 자체(自體)의 형광(螢光)을 이용하여 신속하고 간편하게 보존료(保存料)를 검사(檢査)할 수 있는 방법(方法)을 시도(試圖)하였다. 유동(流動) 파라핀에 현탁(懸濁)된 분말(粉末) 시료(試料)의 흡수(吸收) 및 발광(發光) 스펙트럼은 보존료(保存料)의 종류에 따라 특징적인 패턴을 나타냈다. 장파장(長波長) 측(側) 및 단파장(短波長) 측(側) 영역(領域)의 자외선(紫外線) 조사(照射)에서 얻은 스펙트럼 특성을 대조(對照)하여 비교하므로써 보존료(保存料)의 종류가 보다 용이하게 판별되었다.

• 센서학회지
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• 제14권5호
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• pp.308-312
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• 2005

We present a microfluidic chip designed for the detection of antibody by using quantum dots fluorescence and a microbead-based assay. A custom designed PDMS microfluidic chip with multi-layer channel is utilized for capturing microbeads; antibody injection into each micro-well; QD injection; and fluorescence detection. The experiment using the fabricated microfluidic chip has been performed on solutions with various concentrations of antibody and has shown correlated fluorescent intensities.

• 한국기계가공학회지
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• 제19권12호
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• pp.28-33
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• 2020

Oil-related products have provided many benefits to humanity, but are significant contributors to environmental pollution. As per the International Maritime Organization (IMO) requirements, in the future, all ships must be equipped with filtering equipment and 5ppm bilge alarms that can help remove or reduce oil products during wastewater treatment. In this study, a UV fluorescence measurement system that can detect the oil components in wastewater containing both water and oil was developed. When an excitation wavelength of 254nm was used to irradiate the wastewater, the amount of UV at a divergent wavelength of 360nm was measured to measure the contamination. Based on the measurement, it was concluded that this system is suitable for use as the 5ppm bilge alarm proposed by IMO.

• 한국미생물·생명공학회지
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• 제49권3호
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• pp.316-319
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• 2021

Restriction endonucleases play an important role in molecular cloning, clinical diagnosis, and pharmacological drug studies. In this study, DNA-templated copper nanoclusters (DNA-CuNCs) were used to detect AluI endonuclease activity due to their high fluorescence emission and rapid synthesis of DNA-CuNCs under ambient conditions. Results showed that AluI activity was detected in a highly sensitive manner at low concentrations of AluI endonuclease by the fluorescence intensity of DNA-CuNCs. Additionally, its inhibition was monitored in the presence of daidzein under optimal conditions.

• 대한화학회지
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• 제65권5호
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• pp.397-400
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• 2021

• 한국연소학회:학술대회논문집
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• 한국연소학회 1997년도 제15회 KOSCO SYMPOSIUM 논문집
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• pp.13-21
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• 1997

Excitations of eight pumping transitions for nitric oxide fluorescence imaging are analyzed under equivalent experimental conditions to determine the detection. Frequency mixed dye laser pumping, 1st anti-Stokes $H_2$ Raman of KrF excimer laser pumping and ArF excimer laser pumping show good sensitivities.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2011년도 춘계학술대회 논문집
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• pp.835-836
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• 2011

• Journal of Photoscience
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• 제12권2호
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• pp.87-93
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• 2005

A new assay technique to distinguish between pure compounds and the isomeric mixtures has been suggested using single molecule (SM) fluorescence detection technique. Since the number of emission spots in a fluorophorespread film prepared from a genuine dye solution was determined by experimental condition, the deviation of spot numbers from the expected values could be considered to be an indication of lower purity of the sample solution. The lower limit of sample concentration for this assay was determined to be $5{\times}10^{-10}$ M to show uniform number of expected spots within 10% uncertainties in our experimental condition. An individual fluorescence intensity distribution for a mixture of isomers having doubly different emissivities was simulated by adding distributions obtained from Cy3 and nile red (NR) independently. The result indicated that the mixture could be identified from the pure compounds through the difference in the number of Gaussian functions to fit the distribution. This new assay technique can be applied to the purity test for synthetic biofluorophores which are usually prepared in small quantities not enough for classical ensemble assays.