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http://dx.doi.org/10.48022/mbl.2103.03001

Detection of AluI Endonuclease Activity by Using Double Stranded DNA-Templated Copper Nanoclusters  

Yang, Ji Su (Department of Chemistry, Gachon University)
Gang, Jongback (Department of Chemistry, Gachon University)
Publication Information
Microbiology and Biotechnology Letters / v.49, no.3, 2021 , pp. 316-319 More about this Journal
Abstract
Restriction endonucleases play an important role in molecular cloning, clinical diagnosis, and pharmacological drug studies. In this study, DNA-templated copper nanoclusters (DNA-CuNCs) were used to detect AluI endonuclease activity due to their high fluorescence emission and rapid synthesis of DNA-CuNCs under ambient conditions. Results showed that AluI activity was detected in a highly sensitive manner at low concentrations of AluI endonuclease by the fluorescence intensity of DNA-CuNCs. Additionally, its inhibition was monitored in the presence of daidzein under optimal conditions.
Keywords
Fluorescence assay; DNA-CuNCs; AluI endonuclease assay;
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