• 대한소아치과학회지
• /
• 제49권1호
• /
• pp.65-75
• /
• 2022

본 연구의 목적은 유치의 치아우식증 검사를 위한 정량형광분석(quantitative light-induced fluorescence, QLF) 기술의 유효성을 평가하고, 우식병소의 부피와 QLF 분석결과 사이의 상관관계를 확인하는 것이다. 치아우식증 검사를 위해 53개의 교합면 및 72개의 인접면을 포함한 총 125개의 유구치 치면을 휴대용 QLF 장비를 이용해 조사하여 유구치의 치아우식증을 확인하였다. 또한 치아우식증을 분류하고 우식병소의 부피를 계산하기 위해 micro-CT 방사선 검사를 시행하였다. 상대적으로 낮은 수치를 보였던 인접면 우식에 대한 𝚫R average 결과를 제외하면, QLF 분석결과는 유치의 치아우식증 진단에 대해 충분한 수준의 정확성과 신뢰도를 보였다(민감도 0.75 - 0.94, 특이도 0.82 - 0.95, AUROC 0.88 - 0.98). Spearman 상관분석 결과에서는 𝚫F average 및 QS-Index와 우식병소 부피 사이에 통계적으로 유의한 높은 상관성을 확인할 수 있었다(r = 0.805 - 0.832, p < 0.001). QLF 기술을 이용한 치아우식증 검사는 어린이의 치아우식증 진단에 있어 방사선 노출에 대한 걱정 없이 안전하게 사용 가능하고, 신뢰할 만한 방법이 될 수 있을 것이다.

• 한국우주과학회:학술대회논문집(한국우주과학회보)
• /
• 한국우주과학회 2004년도 한국우주과학회보 제13권1호
• /
• pp.99-99
• /
• 2004

We detected and analyzed molecular hydrogen fluorescence in the Taurus Cloud using the Far-ultraviolet Imaging Spectrograph (FIMS) on the STSAT-1 which was launched at SeP. 27 2003. FIMS is optimized for observing diffuse emission lines in the interstellar medium in the wavelength bands of 900-l150 and 1300-1700 angstrom. The Taurus region is a local molecular cloud which is good for studying molecular hydrogen fluorescence emissions. (omitted)

• 한국식물병리학회지
• /
• 제1권1호
• /
• pp.12-16
• /
• 1985

마이코플라스마에 감염된 대추나무, 뽕나무 및 일일초의 조직절편을 형광염색소인 DAPI(4'-6-diamidino-2-phenylindole$\cdot$2HCl), aniline blue 그리고 quinacrine(quinacrine mustard dihydrochloride)으로 염색하여 형광현미경하에서 관찰함으로서 이들 형광염색소의 마이코프라스마 감염진단에의 효용가치를 비교 조사하였다. 이병식물의 줄기절편의 절부에서 특이형광반응이 나타났으나 건전식물의 조직절편에서는 특이형광반응이 관찰되지 않음으로써, DAPI, anilinc blue 및 quinacrine 등의 형광염색소에 의한 조직염색법은 대추나무, 뽕나무 및 일일초의 마이코플라스마 감염을 식속 정확하게 진단하는 데 매우 유용한 방법임을 보여주었다. 한펀, 이들 형광염색소 중에서는 DAPI가 가장 효과적이었다.

• Journal of Biosystems Engineering
• /
• 제40권4호
• /
• pp.335-344
• /
• 2015

Purpose: Soybean growth is adversely affected by environmental stresses such as drought, extreme temperatures, and nutrient deficiency. The objective of this study was to develop a method for rapid measurement of drought stress in soybean plants using a hyperspectral fluorescence imaging technique. Methods: Hyperspectral fluorescence images were obtained using UV-A light with 365 nm excitation. Two soybean cultivars under drought stress were analyzed. A partial least square regression (PLSR) model was used to predict drought stress in soybeans. Results: Partial least square (PLS) images were obtained for the two soybean cultivars using the results of the developed model during the period of drought stress treatment. Analysis of the PLS images showed that the accuracy of drought stress discrimination in the two cultivars was 0.973 for an 8-day treatment group and 0.969 for a 6-day treatment group. Conclusions: These results validate the use of hyperspectral fluorescence images for assessing drought stress in soybeans.

• 한국연소학회지
• /
• 제12권4호
• /
• pp.1-7
• /
• 2007

Carbon monoxide (CO) is not only an important intermediate species in chemical reaction mechanisms of hydrocarbon fuel combustion, but also a crucial pollutant species emitted from automotive engines. To better understand the physical processes impacting CO emissions, the development of laser-based measurement techniques that can visualize in-cylinder CO distributions is desirable. Among these techniques, Laser-Induced Fluorescence (LIF) is a sensitive and species-selective detection technique capable of good spatial resolution. However, some technical matters such as deep UV excitation, severe pressure dependency of the LIF signal, and potential interference from other species have been major challenges for CO LIF application. This study is focused on investigating the feasibility of CO two-photon LIF in a direct-injection diesel engine operating at typical pressure and temperature conditions with commercial grade diesel fuel. Spectroscopic analysis shows that the CO fluorescence signal can be separated from $C_2$ Swan band or broadband fluorescence from PAHs when the signal is collected near 483 nm. The signal-to-noise ratio of CO LIF deteriorate rapidly as pressure is increased, following $P^{-1.49}$ which matches the theoretical signal pressure dependency.

• 대한화학회지
• /
• 제56권5호
• /
• pp.577-582
• /
• 2012

Quinine quantitative analysis, photo-bleaching, fluorescence quenching, and urine analysis have been performed by means of a UV-LED fluorometer, which can be easily built and used in a high-school laboratory. The quinine detection range is estimated to be 0.05-80 ppm, enough for many classroom luminescence experiments. The quinine content in commercial tonic water is determined from the calibration curve, and UV photo-bleaching of this anti-malarial drug is demonstrated with clear wavelength dependence. Halide quenching of quinine fluorescence is also observed and the increase in quenching efficiency in the order of $Cl^-$, $Br^-$, and $I^-$ is evident. Urine analyses for the student volunteers have been carried out and the results clearly reveal excretion of the ingested quinine. The student participants are exuberant throughout the course of this study and sense the practices resourceful.

• 한국멀티미디어학회논문지
• /
• 제18권12호
• /
• pp.1445-1452
• /
• 2015

In these days, fluorescent materials such as ICG or 5-ALA is used for the brain surgery. The patients who underwent brain tumor surgery has been increased during last 30 years and the survivorship rate increased 22∼33% in 5 years. Recently, the Fluorescence induction surgery is developed for more safety and improved the resection rate for the glioma in the neurosurgery field. In this study, we proposed fluorescence area detection method for ICG and 5-ALA fluorescence induced surgery using acquired images from image processing. Accuracy was 99.21% from ICG images, and 99.51% from 5-ALA images. Matthews correlation coefficient was 88.67% from ICG images, and 90.49% from 5-ALA images.

• 한국축산식품학회지
• /
• 제42권4호
• /
• pp.672-688
• /
• 2022

The objective of this study was to explore the potential of front face fluorescence spectroscopy (FFFS) as rapid, non-destructive and inclusive technique along with multi-variate analysis for predicting meat adulteration. For this purpose (FFFS) was used to discriminate pure minced beef meat and adulterated minced beef meat containing (1%, 2%, 3%, 4%, 5%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, and 100%) of chicken meat as an adulterant in uncooked beef meat samples. Fixed excitation (290 nm, 322 nm, and 340 nm) and fixed emission (410 nm) wavelengths were used for performing analysis. Fluorescence spectra were acquired from pure and adulterated meat samples to differentiate pure and binary mixtures of meat samples. Principle component analysis, partial least square regression and hierarchical cluster analysis were used as chemometric tools to find out the information from spectral data. These chemometric tools predict adulteration in minced beef meat up to 10% chicken meat but are not good in distinguishing adulteration level from 1% to 5%. The results of this research provide baseline for future work for generating spectral libraries using larger datasets for on-line detection of meat authenticity by using fluorescence spectroscopy.

• Archives of Pharmacal Research
• /
• 제15권4호
• /
• pp.328-332
• /
• 1992

A new high performance liquid chromatographic procedure is described for the analysis of ginseng saponins. Ginseng saponins were separated on Lichrosorb $NH_2$ column and anthraquinone-2, 6-disulfonate (AQDS) solution was added to the column effluent. The effluent was passed through 1.5m-PTFE capillary coiled around 10 W-UV lamp to reduce AQDS to highly fluorescent 9. 10-dihydroxyanthracene-2, 6-disulfonate which was detected by fluorescence detector. The detection limit for the ginsenoside $Rg_1$ by this method was found to be about 350 ng, the dynamic linear range was $10^2$ and the correlation coefficient of the calibration curve was 0.9999.

• BMB Reports
• /
• 제46권2호
• /
• pp.65-72
• /
• 2013

In situ detection of RNAs is becoming increasingly important for analysis of gene expression within and between intact cells in tissues. International genomics efforts are now cataloging patterns of RNA transcription that play roles in cell function, differentiation, and disease formation, and they are demon-strating the importance of coding and noncoding RNA transcripts in these processes. However, these techniques typically provide ensemble averages of transcription across many cells. In situ hybridization-based analysis methods complement these studies by providing information about how expression levels change between cells within normal and diseased tissues, and they provide information about the localization of transcripts within cells, which is important in understanding mechanisms of gene regulation. Multi-color, single-molecule fluorescence in situ hybridization (smFISH) is particularly useful since it enables analysis of several different transcripts simultaneously. Combining smFISH with immunofluorescent protein detection provides additional information about the association between transcription level, cellular localization, and protein expression in individual cells.