The aim of this study was to determine the effect of day-length extension treatment using LED lighting (blue, green, red, or 3 mixed) on vegetative growth and flowering of freesia 'Yvonne', in comparison to that using glow lamps and metal halide lamps. Lighting treatments were imposed from 5 PM to 8 PM for 150 days from after flower bud differentiation to flowering end. For the period from leaf emergence to floral bud formation, no light source affected plant height but the number of leaves was decreased by the metal halide lamp. The highest SPAD value in the flower bud developing period occurred in the metal halide lamp treatment. The time of flowering was advanced by blue or green LED treatment compared to the no lighting control. The green LED lighting advanced flowering by 6 and 8 days compared to no lighting and metal halide lamp treatment, respectively. The blooming period tended to be shortened by the red LED treatment. As for the flower characteristics of 'Yvonne', floret length and width, and the weight of cut flowers were highest in the metal halide lamp treatment. Red LED decreased corm width and weight of 'Yvonne' while glow lamp decreased height and weight. Starch contents in corm were not influenced by the lighting source. Our results indicate that the green LED lighting advanced the time of flowering and the metal halide lamp was good for cut-flower quality.
Abnormal chloranthy was observed on Prunus mume trees grown in an orchard in Uiseong, Gyeongbuk province, Korea in 2015. Flower buds showing chloranthy were covered with numerous orange-colored sori, which were confirmed to contain spermogonia and aeciospores by stereo microscopy and light microscopy. When observed using scanning electron microscope, the aeciospores were globose, ellipsoid, or ovoid, and measured $22.8{\sim}35.6{\times}19.8{\sim}36.8{\mu}m$, with a verrucae wall ornamentation. To construct a phylogeny, the large and small subunit regions of rDNA were sequenced and compared with those of allied species by the maximum likelihood method. The isolated fungus species showed high similarities with Caeoma makinoi (Teleomorph: Blastospora smilacis), which was previously isolated from P. mume in Japan. Therefore, we initially report this fungal agent parasitic on P. mume as C. makinoi in Korea and name the disease it causes as "flower bud chloranthy disease".
This experiment was done to determine the optimum conditions for the induction of tetraploidy in Panax ginseng C. A. Meyer using bud length, temperature and plant growth regulator pretreatments. Highest callus formation was obtained when the medium was inoculated with flower bud in the size of 2-3 mm in length. The optimum temperature for the callus formation was high when treated at $4^{\circ}C$ for 4-5 days. Among the treatments of growth regulators and different concentration, highest callus formation was observed in combination of 5 mg/L 2,4-D and 1 mg/L kinetin for P. ginseng. As a result of flow cytometer analysis, all 7 adventitious roots were confirmed as tetraploidys. Cytological analysis revealed that the chromosome number of tetraploid roots was 96, while that of diploid roots was 48. Tetraploid ginseng roots were inoculated to flower bud size of 2-3 mm in length. The callus formation was optimum when treated with 1 mg/L 2,4-D at $4^{\circ}C$ for 5 days. Compared with control roots, tetraploid roots were thicker and longer and had few lateral branches. Fresh weight of tetraploid roots was relatively higher than the control roots.
The present study was conducted to determine the effects of electron beam irradiation on the postharvest quality of cut flowers. Cut flowers were irradiated with electron beam at 100, 200, 400, 600, 800, 1,000, and 2,000 Gy with a 10 MeV linear electron beam accelerator to evaluate their irradiation tolerance. Postharvest quality was determined by monitoring fresh weight loss, flower longevity, flower diameter, flowering rate, visual quality of flowers and leaves, and chlorophyll content. Cut flowers showed a radiation-induced damage with increasing the irradiation dose. Flower longevity and fresh weight of cut flowers decreased when the irradiation dose was increased. Flower bud opening was also inhibited in a dose-dependent manner. The effective irradiation doses for 10% reduction of postharvest quality (ED10) values were 144.4, 451.6, and 841.2 Gy in the 'Medusa' lily, 'Montezuma' carnation, and 'Rosina White' eustoma, respectively. Although tolerance of cut flowers to electron beam irradiation vary according to species, cultivars, or maturity stage conditions, it is conceivable that 'Montezuma' carnation and 'Rosina White' eustoma could be tolerated and maintained overall postharvest quality up to 400 Gy, the generic irradiation dose approved by the Animal and Plant Health Inspection Service (APHIS) and the International Plant Protection Convention (IPPC) for postharvest phytosanitary treatments.
This experiment was conducted to investigate the effect of raising seedling methods of cold water, low night temperature, and abandoned mine on forcing culture of strawberry. The results were as follows ;1. $17^{\circ}C$ cold water alone and cold water plus 8-hour short day treatments significantly accelerated the flower bud differentiation of Nyoho cultivar to 11 and 15 days, respectively, comparing to common soil raising seedling.2. The date of flower bud differentiation of Nyoho cultivar as influenced by cold water treatment the 17th, the 13th and the 10th of September, respectively, when the seedlings were treated on the 10th of September and on the 25th and the 10th of August in 1991. In 1992, data showed that the flower bud differentiation dates were the 4th and the 2nd of September and the 29th of August when cold water treatment was done on the 10th of August and on the 25th and the 10th of July.3. The first harvest date when Nyoho cultivar was treated by cold water for 30days from Jul. 25 was Nov. 10, this implying that the harvesting day could advanced to 19 days comparing to that by the common soil raising seedling method. The resulting yield was recorded to 21.94 ton per hectare.4. Regardless of the starting date of the treatment, $13^{\circ}C$ low night temperature plus 8-plus short day treatments for 20 days required 17 days to differentiate the flower bud of Nyoho cultivar. Harvesting day could be advanced to 59 days comparing to that by the common raising seedling method when the seedling was treated on July 25, this resulting in increasing the total yield obtained by April 14 of following year to 8.25 ton per hectare.5. When the seedlings of both Nyoho and Hokowase cultivars were raised under the condition of abandoned mine, flowering and harvest date were earlier by the treatment for 20 days than that for 30 days or 40 days. The highest yiesd obtained was recorded in Nyoho cultivar or 1.88 ton per hectare.
This study was carried out to ascertain the flowering and boiling habits in conditions of vinyl mulched cultivation. The effects of vinyl mulching cultivation in cotton was early flowering. The flowering period was no more than 26 days and shedding of flower bud was lower. The total boll setting rate to flower number was about 38%. The number of flowering plants was rapidly increased until Aug. 8, then later was decreased, the period of the highest flowering occured was for 5 days from Aug. 6 to Aug. 10. Boll setting was increased that the flowers opened before July 30, then the later flowers was tended to decrease belling in spite of the increased flowering. However flower bud shedding had a tendency to decrease after the highest boiling period. The effect of weather, max. temperature and hours of sunshine on flowering days were highly positive significant correlation. Minimum temperatures, high air humidity and amount of rainfall had a negative significant correlation. The demanded days to flowering on the same internode in each fruiting branches was taken average 1.2 days, these days among the fruiting branches of the same internode was 3.6 days.
Shim Kyung-Ku;Ha Yoo-Mi;Lee Sun A;Park Chang Hyuk;Kim Dong Su
Journal of the Korean Institute of Landscape Architecture
/
v.33
no.5
s.112
/
pp.94-103
/
2005
This study was carried out to investigate growth and morphological characteristics of leaf, flower and winter bud of Corylopsis species such as C. coreana, C. spicata, C. sinensis, C. willmottiae, C. willmottiae 'Spring Purple', C. wilsonii, C. spicata 'Yellow Spring', C. gotoana, and C. vechiana. In the Corylopsis species, C. coreana, C. willmottiae, and C. vetchiana had larger flowers, longer flower cluster, and more flowers than other species. C. sinensis and C. veitchiana had fragrant flowers. In addition, species with beautiful leaf color were C. spicata 'Yellow spring' with yellow color and C. willmottiae 'Spring purple' with purple color, which eventually changed to green. The winter bud of C. sinensis, C. willmottiae, C. willmottiae 'Spring Purple', and C. vechiana were oval and different from those of other species. Comparison of the morphological characteristics among C. coreana, C. spicata, and C. sinensis, C. coreana showed they had earlier flowering dates and brighter yellow flowers than those of other species. Flower cluster of C. sinensis was shortest, but it had most number of flowers and fragrance. In addition, the winter buds of C. coreana and C. spicata with elliptical shape were different from that of C. sinensis with oval shape. Therefore, based on these characteristics, the following Corylopsis species were found to be promising as woody landscape plants: C. coreana, C. sinensis, C. willmottiae 'Spring Purple', C. spicata 'Yellow Spring', and C. vechiana.
Lee, Jin Wook;Park, Gwang Hun;Eo, Hyun Ji;Song, Hun Min;Kim, Mi Kyoung;Kwon, Min Ji;Koo, Jin Suk;Lee, Jeong Rak;Lee, Man Hyo;Jeong, Jin Boo
Korean Journal of Plant Resources
/
v.28
no.3
/
pp.297-304
/
2015
The flower buds of Sophora japonica L (SF), as a well-known traditional Chinese medicinal herb, have been used to treat bleeding-related disorders such as hematochezia, hemorrhoidal bleeding, dysfunctional uterine bleeding, and diarrhea. However, no specific anti-cancer effect and its molecular mechanism of SF have been described. Thus, we performed in vitro study to investigate if treatment of SF affects activating transcription factor 3 (ATF3) expression and ATF3-mediated apoptosis in human colorectal cancer cells. The effects of SF on cell viability and apoptosis were measured by MTT assay and Western blot analysis against cleaved poly (ADP-ribose) polymerase (PARP). ATF3 activation induced by SF was evaluated using Western blot analysis, RT-PCR and ATF3 promoter assay. SF treatment caused decrease of cell viability and increase of apoptosis in a dose-dependent manner in HCT116 and SW480 cells. Exposure of SF activated the levels of ATF3 protein and mRNA via transcriptional regulation in HCT116 and SW480 cells. Inhibition of extracellular signal-regulated kinases (ERK) 1/2 by PD98059 and p38 by SB203580 attenuated SF-induced ATF3 expression and transcriptional activation. Ectopic ATF3 overexpression accelerated SF-induced cleavage of PARP. These findings suggest that SF-mediated apoptosis may be the result of ATF3 expression through ERK1/2 and p38-mediated transcriptional activation.
Rose and chrysanthemum are the most popular flowers in Korean floriculture. Sorting flowers is a labor intensive operation in cultivation of the cut flowers and needed to be mechanized. Machine vision is one of the promising solutions for this purpose. This study was carried out to develop hardware and software of a cut flower sorting system using machine vision and to test its performance. Results of this study were summarized as following; 1. Length of the cut flower measured by the machine vision system showed a good correlation with actual length of the flower at a level of the coefficients of determination (R$^2$) of 0.9948 and 0.9993 for rose and chrysanthemum respectively and average measurement errors of the system were about 2% and 1% of the shortest length of the sample flowers. The experimental result showed that the machine vision system could be used successfully to measure length of the cut flowers. 2. Stem diameter of the cut flowers measured by the machine vision system showed a correlation with actual diameter at the coefficients of determination (R$^2$) of 0.8429 and 0.9380 for rose and chrysanthemum respectively and average measurement errors of the system were about 15% and 7.5% of the shortest diameter of the sample flowers which could be a serious source of error in grading operation. It was recommended that the error rate should be considered to set up grading conditions of each class of the cut flowers. 3. Bud maturity of 20 flowers each judged using the machine vision system showed a coincidence with the judgement by inspectors at ranges of 80%∼85% and 85%∼90% for rose and chrysanthemum respectively. Performance of the machine vision system to judge bud maturity could be improved through setting up more precise criteria to judge the maturity with more samples of the flowers. 4. Quality of flower judged by stem curvature using the machine vision system showed a coincidence with the judgement by inspectors at 90% for good and 85% for bad flowers of both rose and chrysanthemum. The levels of coincidence was considered as that the machine vision system used was an acceptable system to judge the quality of flower by stem curvature.
This study was conducted to improve commercial quality of a cut spray chrysanthemum 'Gama' bred in Korea by foliar application of daminozide (DMZ), suppressing excessive elongation of peduncle caused by high temperature in summer season production. Applications were made at three floral bud developmental stages (I, II, and III), and concentrations used were 0, 500, 1000, and $2000mg{\cdot}L^{-1}$. Both cut flower length and peduncle length showed the greatest suppression of elongation by DMZ treatments at stage II. DMZ also gradually reduced values of those in a concentration-dependant manner. Stem thickness and flower diameter reduced compared to control as DMZ was sprayed at later stage and those two characteristics decreased in a concentration-dependant manner. The number of paralleled flowers neighboring apical part of whole plant showed the best results at stage I and II. As for DMZ concentration, those increased effectively at $500-1,000mg{\cdot}L^{-1}$. Among all combinations, $1,000mg{\cdot}L^{-1}$ DMZ at stage II recorded the most paralleled flowers and showed 24.2% increment as compared to control. The number of fully expanded flowers and total floral buds also showed the highest values using $1,000mg{\cdot}L^{-1}$ DMZ at stage II than the other treatments. In cut spray chrysanthemum cultivar 'Gama' bred in Korea, foliar-spraying with $1,000mg{\cdot}L^{-1}$ DMZ at stage II most effectively suppressed excessive peduncle elongation, parallelled flower cluster arrangement, and increased the number of flowers in summer. However, this combination affected negatively the other commercial qualities, reducing cut flower length, stem thickness, and flower diameter. Therefore, we recommended that foliar-spraying stage and DMZ concentration was stage I and $1,000mg{\cdot}L^{-1}$, respectively, in a practical culture for 'Gama'.
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