• Proceedings of the KIEE Conference
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• 2001.07a
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• pp.362-364
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• 2001

Every engineering discipline has, in recent year, felt the impact of computer-aided analysis and design Normally the studies of power system analysis by computer are load flow, short circuit and harmonic calculation, the amount the voltage deviates from motor starting, reliability and system stability. These studies are very important from the operating and planning points of power system. This paper presents analysis of load flow, short circuit current calculation, the effect for voltage deviation of induction motor starting and harmonic distortion in power system of continuous casting(C. C) plant. The resulting of analysis is applied the configuration and design engineering of power system.

• Journal of the Korean Society of Marine Environment & Safety
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• v.17 no.1
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• pp.75-82
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• 2011

Marine current energy is one of the most interesting renewable and clean energy resources that have been less exploited. Especially, Korea has worldwide outstanding tidal current energy resources and it is highly required to develop tidal in-stream energy conversion system in coastal area. The objective of study is to investigate harnessing techniques of tidal current energy and to design the a 100 kW horizontal axis tidal turbine using blade element momentum theory with Prandtl's tip loss factor for optimal design procedures. In addition, Influence of Prandtl's tip loss factor at local blade positions as a function of tip speed ratio was studied, and the analysed results showed that power coefficient of designed rotor blade using NACA 63812 was 0.49 at rated tip speed ratio.

• Biomolecules & Therapeutics
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• v.26 no.3
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• pp.328-334
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• 2018

Because of the unsatisfactory treatment options for breast cancer (BC), there is a need to develop novel therapeutic approaches for this malignancy. One such strategy is chemotherapy using non-toxic dietary substances and botanical products. Studies have shown that Panduratin A (PA) possesses many health benefits, including anti-inflammatory, anti-bacterial, anti-oxidant and anticancer activities. In the present study, we provide evidence that PA treatment of MCF-7 BC cells resulted in a time- and dose-dependent inhibition of cell growth with an $IC_{50}$ of $15{\mu}M$ and no to little effect on normal human MCF-10A breast cells. To define the mechanism of these anti-proliferative effects of PA, we determined its effect critical molecular events known to regulate the cell cycle and apoptotic machinery. Immunofluorescence and flow cytometric analysis of Annexin V-FITC staining provided evidence for the induction of apoptosis. PA treatment of BC cells resulted in increased activity/expression of mitochondrial cytochrome C, caspases 7, 8 and 9 with a significant increase in the Bax:Bcl-2 ratio, suggesting the involvement of a mitochondrial-dependent apoptotic pathway. Furthermore, cell cycle analysis using flow cytometry showed that PA treatment of cells resulted in G0/G1 arrest in a dose-dependent manner. Immunoblot analysis data revealed that, in MCF-7 cell lines, PA treatment resulted in the dose-dependent (i) induction of $p21^{WAF1/Cip1}$ and p27Kip1, (ii) downregulation of Cyclin dependent kinase (CDK) 4 and (iii) decrease in cyclin D1. These findings suggest that PA may be an effective therapeutic agent against BC.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.3
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• pp.1043-1048
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• 2012

The COPS3 gene has stimulating effect on cell proliferation and progression of osteosarcomas and related cells. However, the features of COPS3 and its potential application as a therapeutic target in other cancers has not yet been studied. In this study, therefore, the effect of COPS3 silencing via COPS3 siRNA on lung cancer cell proliferation was examined. Expression levels of COPS3 gene in COPS3 siRNA infected cells and control siRNA infected cells were compared with real time PCR and Western blot analysis. Cell proliferation levels were comprehensively analyzed by MTT, BrdU incorporationy, and colony formation assays. For mechanistic assessment the effects of COPS3 silencing on cell cycle and apoptosis were analyzed using flow cytometry. Results showed that successful silencing of the COPS3 gene at both translational and transcriptional levels significantly reduced the proliferation and colony formation by lung cancer cells (p<0.01). Flow cytometry showed cell cycle arrest in the G0/G1 phase after COPS3 silencing, and more importantly, apoptosis was induced as a result of COPS3 knockdown, which negatively affected cell survival. Therefore, these results provide another piece of important evidence that the COPS3 gene expressed in lung cancer cells may play a critical role in stimulating proliferation. Down-regulation of COPS3 could significantly inhibit lung cancer cell growth, which was most likely mediated via induction of cell cycle arrest in G0/G1 phase and apoptosis.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.40 no.9
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• pp.613-620
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• 2016

In order to scrutinize the fluid conductivity effects on the electromagnetic flowmeter(EMF) characteristics, a small scale EMF was designed and fabricated. The measuring tube has a $3mm{\times}4mm$ rectangular cross-section, 9 mm length, and a $2mm{\times}3mm$ plate electrode and a ${\Phi}1.5mm$ point electrode. The design parameters, such as the magnetizing frequency and the number of coil turns, and the diameter were optimized. The EMF was tested with a gravimetric calibrator and showed good linearity in the range of 0 to $1.17{\times}10^{-5}m^3/s$. The fluid conductivity was varied between 3 and $11{\mu}S/cm$, and the magnitude of the flow signal was proportional to the fluid conductivity and the wetted area of the electrode. The design information and the test results provide flow measurement techniques for very low flowrate.

• Archives of Pharmacal Research
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• v.29 no.9
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• pp.777-785
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• 2006

Nelumbinis Semen (NS), or lotus seed, is one of the most well-known traditional herbal medicines and is frequently used to treat cardiovascular symptoms in Korea. The anti-ischemic effects of NS on ischemia-induced isolated rat heart were investigated through analyses of changes in blood pressure, aortic flow, coronary flow, and cardiac output. The subjects in this study were divided into two groups: a control, untreated ischemia-induced group, and an ischemia-induced group treated with NS. There were no significant differences in perfusion pressure, aortic flow, coronary flow and cardiac output between the groups before ischemia was induced. The supply of oxygen and buffer was stopped for ten minutes to induce ischemia in isolated rat hearts, and NS was administered during ischemia induction. NS treatment significantly prevented decreases in perfusion pressure, aortic flow, coronary flow and cardiac output under ischemic conditions (p<0.01). In addition, the mechanism of the anti-ischemic effects of NS was also examined through quantitation of intracellular calcium content in rat neonatal cardiomyocytes. NS significantly prevented intracellular calcium increases induced by isoproterenol (p<0.01). These results suggest that NS has distinct anti-ischemic effects through calcium antagonism.

• The KSFM Journal of Fluid Machinery
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• v.11 no.5
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• pp.15-21
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• 2008

In modem wind power system of large capacity above 1MW, horizontal axis wind turbine(HAWT) is a common type. And, the optimum design of wind turbine to guarantee excellent power performance and its reliability in structure and longevity is a key technology in wind Industry. In this study, mathematical expressions based upon the conventional BEMT(blade element momentum theory) applying to basic 1MW wind turbine blade configuration design. Power coefficient and related flow parameters, such as Prandtl's tip loss coefficient, tangential and axial flow induction factors of the wind turbine analyzed systematically. X-FOIL was used to acquire lift and drag coefficients of the 2-D airfoils and we use Viterna-Corrigan formula to interpolate the aerodynamic characteristics in post-stall region. In order to predict the performance characteristics of the blade, a performance analysis carried out by BEMT method. As a results, axial and tangential flow factors, angle of attack, power coefficient investigated in this study.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.23 no.11
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• pp.1453-1463
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• 1999

An electromagnetic flowmeter, based on a magnetic induction principle; provides an obstructionless flowmeter that essentially averages the velocity distribution over the pipe cross-sectional area. To predict the installation effects, the flowmeter installed near $90^{\circ}$ elbow is simulated by using a commercial code FLUENT(ver. 4.48) for the laminar flow field and a code developed through this study for magnetic field. The installation effects of the flowmeter are estimated by varying a number of the dependent parameters such as the radius of the elbow(Rc=1D, 1.5D, 2D, 3D), the location, Reynolds number and the direction of electrodes plane(${\varphi}$). It was found that all these factors affect the performance of the electromagnetic flowmeter significantly. The longer installation distance from the elbow is not always optimal to minimize the error, and also there exists an optimal location to install the EMF for a minimum error. Especially the flow signal with the electrodes plane direction of ${\varphi}=45^{\circ}$ is shown to yield smallest measurement error regardless of the Reynolds number and the curvature of elbow.

• Journal of Advanced Marine Engineering and Technology
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• v.32 no.6
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• pp.827-833
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• 2008

In this study, mathematical expressions based upon the conventional BEMT(blade element momentum theory) was applied to basic 100kW wind turbine blade configuration design. Power coefficient and related flow parameters, such as Prandtl's tip loss coefficient, tangential and axial flow induction factors of the wind turbine were analyzed systematically. X-FOIL was used to acquire lift and drag coefficients of the 2-D airfoils and Viterna-Corrigan formula was used o interpolate he aerodynamic characteristics in post-stall region. Also, aerodynamic characteristics, measured in a wind tunnel to calculate he power coefficient was applied. The comparative results such as axial and tangential flow factors, power coefficients were presented in this study. Power coefficient, calculated by in-house code was compared with the GH-Bladed result. The difference of the aerodynamic characteristics caused the difference of the performance characteristics as variation as TSR.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.5
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• pp.2885-2889
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• 2013

Goniothalamin is an active compound extracted from Goniothalamus griffithii, a local plant found in northern Thailand. Goniothalamin inhibits cancer cell growth but is also toxic to normal cells. The aims of this study were to identify the cytotoxic effect of goniothalamin and the mechanism of cell death in human HL-60 and U937 cells. Cytotoxicity was determined by MTT assay and cell cycle profiles were demonstrated by staining with propidium iodide (PI) and flow cytometry. Apoptosis was confirmed by staining with annexin V-FITC/propidium iodide (PI) and flow cytometry. Reduction of mitochondrial transmembrane potential was determined by staining with dihexyloxacarbocyanine iodide and flow cytometry and expression of Smac, caspase-8 and -9 was demonstrated by Western blotting. Goniothalamin inhibited growth of HL-60 and U937 cell lines. An increase of SubG1 phase was found in their cell cycle profiles, indicating apoptosis as the mode of cell death. Apoptosis was confirmed by the flip-flop of phosphatidylserine using annexin V-FITC/PI assay in HL60 and U937 cells in a dose response manner. Furthermore, reduction of mitochondrial transmembrane potential was found in both cell types while expression of caspase-8, -9 and Smac/Diablo was increased in HL-60 cells. Taken together, our results indicate that goniothalamin-treated human leukemic cells undergo apoptosis via intrinsic and extrinsic pathways.