• Title/Summary/Keyword: flow cell

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Immobilized Luminescent Cell - based Flow Through Monitoring of Environmental Pollutants

  • Britz, Margaret L.;Simonov, Nina;Chun, Uck-Han
    • Journal of Microbiology and Biotechnology
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    • v.7 no.4
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    • pp.250-257
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    • 1997
  • A new sensing system based on the immobilization of luminescent bacteria, photobacterium phosphoreum, was proposed for continuous real-time monitoring of pollutants. The response curves demonstrate that Photobacterium phosphoreum immobilized on the strontium alginate were very sensitive to seven reference chemicals used. The significant inhibitory concentrations for bioluminescence emission were 5 ppm for Pb$(NO_3)_2$), $NiCl_2$, $CdCl_2$, 50 ppm for $NaASO_2$, 0.1 ppm for $HgCl_2$, 0.5 ppm for pentachlorophenol and less than 5 ppm for SDS, respectively. The alginate mixed-cells (AMC) retained their luminescence during experimental period (29 days) under storage condition of $-80^{\circ}C$. The variables affecting performance of continuous flow through monitoring (CFTM) was optimized in order to ensure stability and efficiency. The flow through cell with strontium-alginate immobilized luminescent bacteria was tested with salicylate and 4-nitrophenol. A rapid response of luminescence was recorded by time drive mode in bioluminescence spectrometer after exposure to both toxicants.

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The Effects of Jujadokseo-hwan on the Activation of Brain and Neuroprotactive Effects (주자독서환의 뇌기능 활성 및 신경세포 보호효과)

  • Lee, Yu-Gyung;Chae, Jung-Won
    • The Journal of Pediatrics of Korean Medicine
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    • v.23 no.3
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    • pp.241-262
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    • 2009
  • Objectives This study is designed to investigate the effects of Jujadokseo-hwan on the brain ability and inducing oxidative stresses. Methods We measured the changes of regional cerebral blood flow and mean arterial blood pressure. Then we analyzed histological examination, immunohistochemistric response and anti-oxidant activity of Jujadokseo-hwan. Results 1. Treatment of Jujadokseo-hwan significantly increased regional cerebral blood flow but decreased mean arterial blood pressure. 2. Treatment of Jujadokseo-hwan-induced increase of regional cerebral blood flow was significantly inhibited by pretreatment with indomethacin (1 mg/kg, i.p.), an inhibitor of cyclooxygenase. 3. In histological examination through TTC stain, group I was no change, but group II showed that discolored in the most cortical part. Group III showed that decreased discolor in the cortical part. 4. In immunohistochemistric response of BDNF, group II showed that lower response effect. Group III showed that increase response effect. 5. Treatment of Jujadokseo-hwan increased proliferation rates of Glial cell effectively 6. Treatment of Jujadokseo-hwan accelerated proliferation rates of C6 cells in vitro. In addition, protective effects on cell death induced by paraquat, rotenone and hydrogen peroxide. In addition, activity of SOD were increased by treatment with Jujadokseo-hwan. Conclusions In conclusion, Jujadokseo-hwan can improve of the brain ability, learning ability, memory ability and induce ischemic brain injuries.

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DEVELOPMENT OF A 2-D GAS-KINETIC BGK SOLVER FOR CONTINUUM AND TRANSITIONAL FLOWS ON UNSTRUCTURED MESHES (비정렬 격자계에서 연속체 및 천이 영역 유동 해석을 위한 2차원 Gas-Kinetic BGK 해석자 개발)

  • Yang, T.H.;Kwon, O.J.
    • Journal of computational fluids engineering
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    • v.19 no.2
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    • pp.49-57
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    • 2014
  • In the present study, 2-D gas-kinetic flow solver on unstructured meshes was developed for flows from continuum to transitional regimes. The gas-kinetic BGK scheme is based on numerical solutions of the BGK simplification of the Boltzmann transport equation. In the initial reconstruction, the unstructured version of the linear interpolation is applied to compute left and right states along a cell interface. In the gas evolution step, the numerical fluxes are computed from the evaluation of the time-dependent gas distribution function around a cell interface. Two-dimensional compressible flow calculations were performed to verify the accuracy and robustness of the current gas-kinetic approach. Gas-kinetic BGK scheme was successfully applied to two-dimensional steady and unsteady flow simulations with strong contact discontinuities. Exemplary hypersonic viscous simulations have been conducted to analyze the performances of the gas-kinetic scheme. The computed results show fair agreement with other standard particle-based approaches for both continuum part and transitional part.

$^{99m}Tc-Red$ Blood Cell Scintigraphy of Sonographically Atypical Hemangioma (초음파상 비전형적 소견을 보이는 간혈관종의 적혈구 표지 스캔소견)

  • Lee, Kyoung-Soo;Lee, Ji-Young;Kim, Chan-Soo;Kim, Chang-Guhn;Choi, See-Sung;Won, Jong-Jin
    • The Korean Journal of Nuclear Medicine
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    • v.26 no.2
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    • pp.338-345
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    • 1992
  • We evaluated the scintigraphic findings of sonographically atypical hemangioma, that is, a hypoechoic or heterogeneously hypoechoic nodule which contains hypoechoic areas more than 40% of the volume, and compared it with those of sonographically typical hemangioma in 26 patients with 31 nodules. Sonographically atypical hemangioma were 13 in 11 patients and sonographically typical hemangioma were 18 the 15 patients. Dynamic blood flow, planar and SPECT blood pool imaging was performed. In atypical hemangioma, increased blood pool activity was seen in 11 of 13 nodules on SPECT and in 10 of 13 nodules on planar image and increased blood flow was seen in 4 of 13 nodules on dynamic blood flow study. No significant difference in detecting increased blood pool activity between sonographically atypical and typical hemangioma. In conclusion, $^{99m}Tc-red$ blood cell SPECT is as useful in diagnosis of sonographically atypical hemangioma as in typical hemangioma and can be used as a confirmatory or complementary study.

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Characterization of Spherical NiO-YSZ Anode Composites for Solid Oxide Fuel Cells Synthesized by Ultrasonic Spray Pyrolysis

  • Lim, Chae-Hyun;Lee, Ki-Tae
    • Journal of the Korean Ceramic Society
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    • v.51 no.4
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    • pp.243-247
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    • 2014
  • Spherical NiO-YSZ particles were synthesized by ultrasonic spray pyrolysis (USP). The morphology of the synthesized particles can be modified by controlling parameters such as precursor pH, carrier-gas flow-rate, and temperature of the heating zone. The synthesized spherical NiO-YSZ particles have rough surface morphology at high carrier-gas flow-rates due to rapid gas exhaustion and insufficient particle ordering. The Ni-YSZ cermet anode synthesized by ultrasonic spray pyrolysis at a flow rate of l L/min, with precursor solution at pH4, showed a higher maximum power density of 256 $mW/cm^2$ compared to a conventionally mixed Ni-YSZ anode (185 $mW/cm^2$) at $800^{\circ}C$. While the area-specific resistance of conventionally mixed Ni-YSZ anodes increases gradually with operation time (indicating performance degradation), the Ni-YSZ anode synthesized by USP does not exhibit any performance degradation, even after 500 h.

A Study on the Characteristics of the Flow around a Sunken Vessel (침선어초 주위의 유동특성에 관한 연구)

  • 양찬규;김현주
    • Journal of Ocean Engineering and Technology
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    • v.14 no.4
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    • pp.9-16
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    • 2000
  • This paper deals with the numerical and experimental study on the characteristics of the flow around a sunken vessel. Numerical simulation of the two dimensional steady flow on the midship section are carried out by the CFD code which is developed by using finite volume method and which includes the standard $textsc{k}$-$\varepsilon$ model with standard wall function. A experimental study is also carried out for the 1/100 scale model in circulating water channel. A velocity fields around the ship are measuremed by using particle image velocimetry technique. And the fluid forces acting on the ship hull by uniform current are measured by two axis load cell. The computed and measured velocity fields on the midship section are compared with each other in the view point of velocity dstribution and reattachement length, which shows good agreement in quality. The drag force on the vessel also showed the same tendency in both computational and experimental results. However, the quantitative disagreements are shown due to the three dimensional effect of the experiment. The result are used to determine the functional efficiency and stability of the vessel as a artificial reef.

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Numerical Study of Land/Channel Flow-field Optimization in Polymer Electrolyte Fuel Cells (PEFCs) (I) -The Effects of Land/Channel Flow-field on Current Density and HFR Distributions- (고분자전해질형연료전지의 가스 채널 최적화를 위한수치적연구(I) -가스 채널 치수가 전류밀도와 HFR 분포에 미치는영향성-)

  • Ju, Hyun-Chul
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.32 no.9
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    • pp.683-694
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    • 2008
  • The performance and durability of Polymer Electrolyte Fuel Cells (PEFCs) are strongly influenced by the uniformity of current density, temperature, species distributions inside a cell In order to obtain uniform distributions in them, the optimal design of flowfield must be a key factor. In this paper, the numerical study of land/channel flowfield optimizations is performed, using a multi-dimensional, multi-phase, non-isothermal PEFC model. Numerical simulations reveal more uniform current density and HFR(High Frequency Resistance) distributions and thus better PEFC performance with narrower land/channel width where the less severe oxygen depletion effect near the land region and more uniform contact resistance variation along the in-plane direction are achieved. The present study elucidates detailed effects of land/channel width and assist in identifying optimal flow-field design strategies for the operation of PEFCs.

Numerical study on PEM fuel cell performance with variation of GDL permeability and channel geometry (기체확산층의 유동투과율과 채널 형상 변화에 따른 고분자 전해질 연료전지 성능변화 수치연구)

  • Koh, Soo-Gon;Sohn, Sang-Ho;Nam, Jin-Hyun;Kim, Charn-Jung
    • Proceedings of the KSME Conference
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    • 2008.11b
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    • pp.3114-3119
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    • 2008
  • Relatively high convective flow exists in the under-rib regions of a gas diffusion layer (GDL) when serpentine flow fields are employed in a PEMFC. This under-rib convection is believed to be favorable for the performance of PEMFCs, by enabling more effective use of catalysts in the under-rib regions. From the fact that the under-rib convection in a GDL is directly proportional to the permeability of the GDL, computational fluid dynamics (CFD) simulations were performed to discover the relationship between the GDL permeability and the PEMFC performance. Single-, triple-, and quintuple-path parallel serpentine flow fields for $9\;cm^2$ active cell area were considered while changing the GDL permeability from $1{\times}10^{-12}$ to $5{\times}10^{-11}m^2$. The results showed that higher GDL permeability generally resulted in better performance of PEMFCs, but the degree of performance enhancement became smaller as the parallel path number increased. The effects of the permeability on the local variables were also discussed.

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Computational fluid dynamics analysis on the effect of inlet humidity for the performance of PEMFC with serpentine flow-fields (입구 가습량이 고분자 전해질 연료전지의 성능에 미치는 영향에 대한 CFD 해석연구)

  • Oh, Gyu-Hwan;Lee, Kyu-Jin;Nam, Jin-Hyun;Kim, Charn-Jung
    • Proceedings of the KSME Conference
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    • 2008.11b
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    • pp.2828-2833
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    • 2008
  • Water management is one of many operating parameters, which influences the performance and stability of a proton exchange membrane fuel cell (PEMFC). Local humidity condition including liquid water saturation has profound impacts on the distributions of overpotentials, current density, and membrane water content. Computational fluid dynamics simulations were conducted to investigate the effect of the inlet humidity variation on the performance of a PEMFC of $9\;cm^2$ active cell area with serpentine flow fields. The results showed that the performance of the simulated PEMFC remained at an almost same level when the cathode inlet humidity was changed from 100% to 60%, while reaching its maximum at air humidity of 80%. However, further decrease in the cathode inlet humidity below 40% started to significantly deteriorate the performance of the PEMFC. The variations of overpotentials, membrane water content, etc. due to the change in the cathode inlet humidity were also discussed.

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Boundary Layer Flow Under a Sluice Gate (수직수문하의 경계층흐름)

  • 이정열
    • Water for future
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    • v.27 no.3
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    • pp.95-105
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    • 1994
  • The boundary layer flow under a sluice gate is numerically solved by the random vortex sheet method combined with the vortex-in-cell method in a boundary-fitted coordinate system. The numerical solution shows that the boundary layer developed along the vertical sluice gate wall is the primary cause for the discrepancy in the contraction ratio between the laboratory experiments and inviscid theory; the bottom boundary layer plays much a smaller role in the discrepancy. By dimensional analysis it is concluded that the discrepancy is inversely proportional to the 3/4th power of the gate opening, as analyzed by Benjamin(1956). The results of the numerical simulation and dimensional analysis show a good agreement with experimental results obtained by Benjamin(1956).

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