The Journal of Pediatrics of Korean Medicine (대한한방소아과학회지)

The Association of Korean Oriental Pediatrics (대한한방소아과학회)
권호 표지

The Effects of Jujadokseo-hwan on the Activation of Brain and Neuroprotactive Effects

주자독서환의 뇌기능 활성 및 신경세포 보호효과

  • Lee, Yu-Gyung (Department of Pediatrics, College of Oriental Medicine, Dongshin University) ;
  • Chae, Jung-Won (Department of Pediatrics, College of Oriental Medicine, Dongshin University)
  • 이유경 (동신대학교 한의과대학 소아과학교실) ;
  • 채중원 (동신대학교 한의과대학 소아과학교실)
  • Published : 2009.12.31
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Abstract

Objectives This study is designed to investigate the effects of Jujadokseo-hwan on the brain ability and inducing oxidative stresses. Methods We measured the changes of regional cerebral blood flow and mean arterial blood pressure. Then we analyzed histological examination, immunohistochemistric response and anti-oxidant activity of Jujadokseo-hwan. Results 1. Treatment of Jujadokseo-hwan significantly increased regional cerebral blood flow but decreased mean arterial blood pressure. 2. Treatment of Jujadokseo-hwan-induced increase of regional cerebral blood flow was significantly inhibited by pretreatment with indomethacin (1 mg/kg, i.p.), an inhibitor of cyclooxygenase. 3. In histological examination through TTC stain, group I was no change, but group II showed that discolored in the most cortical part. Group III showed that decreased discolor in the cortical part. 4. In immunohistochemistric response of BDNF, group II showed that lower response effect. Group III showed that increase response effect. 5. Treatment of Jujadokseo-hwan increased proliferation rates of Glial cell effectively 6. Treatment of Jujadokseo-hwan accelerated proliferation rates of C6 cells in vitro. In addition, protective effects on cell death induced by paraquat, rotenone and hydrogen peroxide. In addition, activity of SOD were increased by treatment with Jujadokseo-hwan. Conclusions In conclusion, Jujadokseo-hwan can improve of the brain ability, learning ability, memory ability and induce ischemic brain injuries.

Keywords

References

  1. Raymond D. Adams Maurice Victor Allan H. Ropper. 신경과학. 서울: 정담. 1998:715-61.
  2. 전국한의과대학 병리학교실. 한방병리학. 제천:한의문화사. 2001:27-31, 43-6, 69-81, 165-72.
  3. 조남근. 족삼리 자침이 혈압과 뇌혈류역학에 미치는 영향. 대한침구학회지. 1999;16(4):307-19.
  4. 조남근. 용천혈의 자침 및 애구 시술이 혈압과 국소뇌혈류량에 미치는 영향. 대한침구학회지. 1998;15(2):227-36.
  5. 배병철 역. 금석황제내경소문. 서울:성보사. 1994:118-9,383-4,628.
  6. 허준. 동의보감.서울:법인문화사; 1999:191.
  7. 허준. 동의보감.서울:법인문화사; 1999:190.
  8. 강혁수, 임강현, 고흥. 주자독서환의 아밀로이드베타로 유발된 생쥐 알츠하이머모델에 대한 효과. 대한한방내과학회지. 2006;27(1):253-64.
  9. 강인탁. 알코올 중독에 대한 주자독서환의 학습능력향상 및 간손상회복 효과. 우석대학교 대학원논문집. 2007.
  10. Bederson J.B., Pitts L.H., Tsuji M., Nishimura M.C., Davis R.L. & Bartkowski H. Rat middle cerebral artery occlusion: evaluation of the model and development of a neurologic examination. Stroke. 1996;17(3):472-6.
  11. Nagasawa H. & Kogure K. Correlation between cerebral blood flow and histologic changes in a new rat model of middle cerebral artery occlusion. Stroke. 1989;20(8):1037-43. https://doi.org/10.1161/01.STR.20.8.1037
  12. Mari M, Seiji K, Kenji F, Airo T and Toshimasa N. Evaluation of the estrogenic activities of some pesticides and their combinations using MtT/Se cell proliferation assay. International Journal of Hygiene and Environmental Health. 2006;209(5):413-21. https://doi.org/10.1016/j.ijheh.2006.04.004
  13. Lockhart BP, Lestage PJ. Conition enhancing or neuroprotective compounds for the treatment of cognitive disorders. why? when? which? Exp Gerontol. 2003;38(1-2):119-28 https://doi.org/10.1016/S0531-5565(02)00163-8
  14. Simard M, van Reekum R. Memory assessment in studies of cognition-enhancing drugs for Alzheimer's disease. Drugs Aging. 1999;14(3):197-230. https://doi.org/10.2165/00002512-199914030-00004
  15. 김은선, 박동운, 이종은. 뇌질환 치료제 I , 산업시장분석. 서울:한국과학기술정보연구원. 2006:25-55.
  16. 나영신. 뇌혈관질환에서 SPECT와 PET의 임상적 응용. 대한핵의학회지.2003;37(1):1-12.
  17. 대한신경외과학회. 신경외과학. 서울:중앙문화사. 1998:150-6,275-6.
  18. 이시진. 본초강목중국의학대계. 서울:여강출판사. 1995:650-1.
  19. 손사막. 비급천금요방. 중국의학대계. 서울:여강출판사. 1995:896-8.
  20. 허준. 동의보감.서울:법인문화사. 1999:487.
  21. 이천. 의학입문. 서울:의성당. 1994:126.
  22. 황도연. 신증방약합편. 서울:영림사. 2002:376, 388, 407, 439, 442, 489, 515, 533.
  23. Giacobini E. From molecular structure to Alzheimer therapy. Jpn J Pharmacol. 1997;74(3):225-41. https://doi.org/10.1254/jjp.74.225
  24. Giacobini E. Present and future of Alzheimer therapy. J Neural Transm Suppl. 2000;59:231-42.
  25. Bachurin SO. Medicinal chemistry approaches for the treatment and prevention of Alzheimer's disease. Med Res Rev. 2003;23(1):48-88. https://doi.org/10.1002/med.10026
  26. Harris RJ, Symon L, Branston NM, Bayhan M. Changes in extracellular calcium activity in cerebral ischemia, J.Cereb Blood Mrtab. 2001:203-9.
  27. 김우겸. 인체의 생리. 서울:서울대학교 출판부. 1985:20-47,107-118.
  28. Wang Q, Pelligrino DA, Paulson OB, Lassen NA. Comparison of the effects of NG-nitro-L-arginine and indomethacin on hypercapnic cerebral blood flow increase in rats. Brain Res. 1994;641(2):257-264. https://doi.org/10.1016/0006-8993(94)90152-X
  29. Iwamoto J, Yoshinaga M, Yang SP, Krasney, J.Methylene blue inhibits hypoxic cerebral vasodilation in awake sheep, J.Appl Physiol. 1992;73(6):2226-32.
  30. Okura Y, Takeda K, Honda S, Hanawa H, Watanabe H, Kodama M, Izumi T, Aizawa Y, Seki S, Abo T. Recombinant murine interleukin-12 facilitates induction of cardiac myosin-specific type 1 helper T cells in rats. Circ Res. 1998;82(10)1035-42. https://doi.org/10.1161/01.RES.82.10.1035
  31. Ginsberg MD. Neuroprotection for ischemic stroke: past, present and future. Neuropharmacology. 2008;55(3):363-89. https://doi.org/10.1016/j.neuropharm.2007.12.007
  32. Buchan AM, Pulsinelli WA. Hypothermia but not the N-methyl-D-aspartate receptor antagonist, MK-801, attenuates neuronal damage in gerbils subjected to transient global ischemia. J Neurosci, 1990;11:1049-50.
  33. Aronowski J., Ostrow P., Samways E., Strong R., Zivin J.A. & Grotta J.C. Graded bioassay for demonstration of brain rescue from experimental acute ischemia in rats. Stroke. 1994;25(11):2235-2240. https://doi.org/10.1161/01.STR.25.11.2235
  34. Goldlust E.J., Paczynski P.P., He Y.Y., Hsu C.Y. & Goldberg M.P. Automated measurement of infarct size with scanned images of triphenyltetrazolium chloride-stained rat brains. Stroke. 1996;27(9): 1657-62. https://doi.org/10.1161/01.STR.27.9.1657
  35. Kerege F, Bondilfi G, Gervasoni N, Schwald M, Aubry JM, Bertschy G. Low brain-derived neurotrophic factor(BDNF) levels in serum of depressed patients probably results from lowered platelet BDNF release unrelated to platelet activity. Biol Psychiatry, 2005;57:1068-72. https://doi.org/10.1016/j.biopsych.2005.01.008
  36. Connor B, Dragunow M. The role of neuronal growth factors in neurodegenerative disorders of the human brain. Brain Res Reviews. 1998;27(1):1-39. https://doi.org/10.1016/S0165-0173(98)00004-6
  37. Legutko B, Li X, Skolnick P. Regulation of BDNF expressionin primary neuron culture by LY392098, a novel AMPA receptor potentiator. Neuropharmachology. 2001;40(8):1019-27. https://doi.org/10.1016/S0028-3908(01)00006-5
  38. Cotman CW, Berchtold NC. Exercise:a behavioral intervention to enhance brain health and plasticity. Trends Neurosci. 2002;25(6):295-301. https://doi.org/10.1016/S0166-2236(02)02143-4
  39. Gomez-Pinilla F, Vaynman S. A "deficient enviroment" in prenatal life may compromise systems important for cognitive function by affecting BDNF in the hippocampus. Exp Neurol. 2005;192:235-43. https://doi.org/10.1016/j.expneurol.2004.12.001
  40. Eichenbaum H. The hippocampus and declaractive memory:cognitive mechanism and neural codes. Behav Brain Res. 2000;127:199-207. https://doi.org/10.1016/S0166-4328(01)00365-5
  41. Wills BK, Aks S, Maloney GE, Rhee J, Brand R, Sekosan M. The effect of amifostine, a cytoprotective agent, on paraquat toxicity in mice. J Med Toxicol. 2007;3(1):1-6. https://doi.org/10.1007/BF03161031
  42. Krasowska A, Sigler K. Cell-protective and antioxidant activity of two groups of synthetic amphiphilic compounds-phenolics and amine N-oxides. Folia Microbiol. 2007;52(6):585-92. https://doi.org/10.1007/BF02932187
  43. Mullens W, Abrahams Z, Francis GS, Skouri HN, Starling RC, Young JB, Taylor DO, Tang WH. Sodium nitroprusside for advanced low-output heart failure. J Am Coll Cardiol. 2008; 15:52(3):200-7.
  44. Molina-Jiménez MF, Sánchez-Reus MI, Andres D, Cascales M, Benedi J. Neuroprotective effect of fraxetin and myricetin against rotenone-induced apoptosis in neuroblastoma cells. Brain Res. 2004 ;1009(1-2):9-16. https://doi.org/10.1016/j.brainres.2004.02.065
  45. Zhang JG, Nicholls-Grzemski FA. Tirmenstein MA, Fariss MW. Vitamin E succinate protects hepatocytes against the toxic effect of reactive oxygen species generated at mitochondrial complexes I and III by alkylating agents. Chem Biol Interact. 2001;138(3):267-84. https://doi.org/10.1016/S0009-2797(01)00278-2
  46. Corpas FJ et al. The expression of different superoxide dismutase forms is cell-type dependent in olive (Olea europaea L.) leaves. Plant Cell Physio. 2006;47(7):984–94. https://doi.org/10.1093/pcp/pcj071
  47. Corpas FJ, Barroso JB, del Río LA. Peroxisomes as a source of reactive oxygen species and nitric oxide signal molecules in plant cells. Trends Plant Sci. 2001;6(4):145-50. https://doi.org/10.1016/S1360-1385(01)01898-2
  48. Pompella A, Visvikis A, Paolicchi A, De Tata V, Casini AF. The changing faces of glutathione, a cellular protagonist. Biochem Pharmacol. 2003;66(8):1499–503. https://doi.org/10.1016/S0006-2952(03)00504-5
  49. Pastore A, Piemonte F, Locatelli M, Lo Russo A, Gaeta LM, Tozzi G, Federici G. Determination of blood total, reduced, and oxidized glutathione in pediatric subjects. Clin. Chem. 2003;47(8):1467–9.