• Proceedings of the KSME Conference
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• 2008.11a
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• pp.1690-1691
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• 2008

Micro cell stimulation device is interested in many researchers because it has several advantages such as saving time and reagents. We introduce new micro-bioreactor using micro bead and conduct cell stimulation experiments to verify effective time because cell have operated by cell-cycle (G1, S, G2, and M phase). Micro-bioreactor was made by soft lithography and CAPE (calf pulmonary artery endothelial cell) was cultured in PDMS (polydimethylsiloxane) micro device for 12 hour and cell starvation process was performed for 24 hours. Micro glass beads were rolled only by slating device every hour during 15 hour because of minimizing other stimulation force like flow and pressure. The result represents that cells under exposed under micro bead stimulation show higher growth rate than normal condition and earlier and later stimulation time are more effective.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.4
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• pp.1655-1659
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• 2016

Celecoxib, a selective inhibitor of COX-2, showed cytotoxic effects in many cancer cell lines including cervical cancer cells. This study investigated the effect of celecoxib on cell cycle arrest in HeLa cervical cancer cells through p53 expression. In vitro anticancer activity was determined with the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) method. A double staining method was applied to investigate the mechanism of cell death, cell cycling was analyzed by flow cytometryand immunocytochemistry was employed to stain p53 expression in cells. Celecoxib showed strong cytotoxic effects and induced apoptosis with an $IC_{50}$ value of $40{\mu}M$. It induced cell cycle arrest at G2/M phase by increasing level of p53 expression on HeLa cells.

• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.3
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• pp.163-170
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• 2002

This report describes the use of a transtubular bioreactor to study the relative effects of diffusion versus perfusion of medium on antibody production by a hybridoma cell line. The study was performed with a high-density cell culture maintained in a serum-free, low-protein medium for 77 days. It was determined that the reactor possessed a macro-mixing pattern residence time distribution similar to a continuous stirred tank reactor (CSTR), However, due to the arrangement of the medium lines in the reactor, the flow patterns for nutrient distribution consist of largely independent medium path lengths ranging from short to long. When operated with cyclic, reversing, transtubular medium flow, some regions of the reactor (with short residence times) are more accessible to medium than others (with long residence times). From this standpoint, the reactor can be divided into three regions: a captive volume, which consists of medium primarily delivered via diffusion; a lapped volume, which provides nutrients through unilateral convection; and a swept volume, which operates through bilateral convection. The relative sizes of these three volumes were modified experimentally by changing the period over which the direction of medium flow was reversed from 15 min (larger captive volume) to 9 h (larger swept volume). The results suggest that antibody concentration increases as the size of the diffusion-limited (captive) volume is increased to a maximum at around 30 min with a sharp decrease thereafter. As reflected by changes in measured consumption of glucose and production of lactate, no significant difference in cellular metabolism occurred as the reactor was moved between these different states. These results indicate that the mode of operation of the transtubular bioreactor may influence antibody productivity under serum-free, low-protein conditions with minimal effects on cellular metabolism.

• Journal of Advanced Marine Engineering and Technology
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• v.35 no.6
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• pp.765-772
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• 2011

In this research, the fuel cell system model capable of generating codes in real time was developed to construct of a HIL (Hardware-In-the-Loop) for a SOFC-powered ship. Moreover, the effects of the distribution of the exhaust gas flow rates in a stack, the flow rates of fuels and temperature of air supplied on the temperature characteristics of fuels supplied to the cathode and the anode, the output power of the stack and system efficiency are examined to minimize the temperature difference between fuels supplied to the stack used in a 500kW SOFC system using methane as a fuel. As a result, the temperatures of fuels supplied to the cathode and the anode maintain at 830K when the opening factor of three-way valve located at outlet of turbine is 0.839. Also the process for optimization of methane flow rate considering the fuel cell stack and system efficiency is required to increase the temperatures of fuels supplied to the stack.

• Journal of the Korean GEO-environmental Society
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• v.13 no.10
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• pp.63-68
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• 2012

This study was performed to determine optimum conditions of batch type cultivation of Isochrysis galbana cultivated under various wavelengths of light emitting dioes (LEDs). Among LEDs used in the cultivation, white LED was found to be the most effective light source, and light intensity of 3,000Lux resulted in the most effective for the cultivation of Isochyrysis galbana. Comparison with common light source, fluorescent light, showed less effective than that with white LED. Four different air flow rates were tested to overcome shading effects due to denser cell concentration in the solution. In results, cell growth rates and maximum cell concentrations were similar regardless of air flow rates. Three times greater cell concentrations, however, were observed when air was applied.

• 한국태양에너지학회:학술대회논문집
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• 2011.11a
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• pp.154-157
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• 2011

Hydrogenated silicon nitride deposited by LF-PECVD is commonly used for anti-reflection coating and passivation in silicon solar cell fabrication. The deposition of the optimized silicon nitride on the surface is elemental in crystalline silicon solar cell. In this work, the carrier lifetimes were measured while the thicknesses of $SiN_x$ were changed from 700 ${\AA}$ to 1150 ${\AA}$ with the gas flow of $SiH_4$ as 40 sccm and $NH_3$ as 120 sccm,. The carrier lifetime enhanced as the thickness of $SiN_x$ increased due to improved passivation effect. To study the characteristics of $SiN_x$ with various gas ratios, the gas flow of $NH_3$ was changed from 40 sccm to 200 sccm with intervals of 40 sccm. The thickness of $SiN_x$ was fixed as 1000 ${\AA}$ and the gas flow of $SiH_4$ as 40 sccm. The refractive index of SiNx and the carrier lifetime were measured before and after heat treating at $650^{\circ}C$ to investigate their change by the firing process in solar cell fabrication. The index of refraction of SiNx decreased as the gas ratios increased and the longest carrier lifetime was measured with the gas ratio $NH_3/SiH_4$ of 3.

• Transactions of the Korean hydrogen and new energy society
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• v.27 no.1
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• pp.49-62
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• 2016

In this study, a solid oxide fuel cell (SOFC) system model including balance of plant (BOP) for building electric power generation is developed to study the effect of operating conditions on the system efficiency and power output. SOFC system modeled in this study consists of three heat-exchangers, an external reformer, burner, and two blowers. A detailed computational cell model including internal reforming reaction is developed for a planer SOFC stack which is operated at intermediate temperature (IT). The BOP models including an external reformer, heat-exchangers, a burner, blowers, pipes are developed to predict the gas temperature, pressure drops and flow rate at every component in the system. The SOFC stack model and BOP models are integrate to estimate the effect of operating parameters on the performance of the system. In this study, the design of experiment (DOE) is used to compare the effects of fuel flow rate, air flow rate, air temperature, current density, and recycle ratio of anode off gas on the system efficiency and power output.

• Applied Chemistry for Engineering
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• v.22 no.5
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• pp.518-521
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• 2011

A PEMFC single cell with the active area of $25cm^2$ was used to verify the effect of water management in the anode. Water management is one of the most critical operating variables. In this paper the effect of operating condition change, such as anode humidification and temperature, was investigated under constant current density of $200mA/cm^2$ where possible anode flooding operating area. Also experiments to observe the effect of the anode and cathode stoichiometry change and flow field design on the water management were performed. The water management was effected by the stoichimetry change. The temperature and humidification change also affected the fuel cell performance.

• 한국전기화학회:학술대회논문집
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• 2002.07a
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• pp.147-150
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• 2002

• 한국전산유체공학회:학술대회논문집
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• 2008.08a
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• pp.43.2-43.2
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• 2008