• Journal of Mechanical Science and Technology
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• v.20 no.12
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• pp.2218-2229
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• 2006

A conservative finite-volume numerical method for unstructured grids with the cell-centered method has been developed for computing flow and heat transfer by combining the attractive features of the existing pressure-based procedures with the advances made in unstructured grid techniques. This method uses an integral form of governing equations for arbitrary convex polyhedra. Care is taken in the discretization and solution procedure to avoid formulations that are cell-shape-specific. A collocated variable arrangement formulation is developed, i.e. all dependent variables such as pressure and velocity are stored at cell centers. For both convective and diffusive fluxes the forms superior to both accuracy and stability are particularly adopted and formulated through a systematic study on the existing approximation ones. Gradients required for the evaluation of diffusion fluxes and for second-order-accurate convective operators are computed by using a linear reconstruction based on the divergence theorem. Momentum interpolation is used to prevent the pressure checkerboarding and a segregated solution strategy is adopted to minimize the storage requirements with the pressure-velocity coupling by the SIMPLE algorithm. An algebraic solver using iterative preconditioned conjugate gradient method is used for the solution of linearized equations. The flow analysis code (PowerCFD) developed by the present method is evaluated for its application to several 2-D structured-mesh benchmark problems using a variety of unstructured quadrilateral and triangular meshes. The present flow analysis code by using unstructured grids with the cell-centered method clearly demonstrate the same accuracy and robustness as that for a typical structured mesh.

• Korean Journal of Microbiology
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• v.55 no.3
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• pp.199-205
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• 2019

Yeast two-hybrid (Y2H) technique has been used to study protein-protein interactions, but its application particularly to a large-scale analysis of protein interaction networks, is limited by the fact that the technique is labor-intensive, based on scoring colonies on plate. Here, we develop a new reporter for the measurement of the protein-protein interactions by flow cytometry. The yeast harboring interacting proteins can also be enriched by fluorescence-activated cell sorting (FACS) or magnetic-activated cell sorting (MACS). When two interacting proteins are present in the same yeast cell, a reporter protein containing 10 tandem repeats of c-myc epitope becomes localized on the surface of the cell wall, without affecting cell growth. We successful measured the surface display of c-myc epitope upon interacting p53 with SV40 T antigen by flow cytometry. Thus, the newly developed Y2H assay based on the display of c-myc repeat on yeast cell wall could be used to the simultaneous analysis of multiple protein-protein interactions without laborious counting colonies on plate.

• New & Renewable Energy
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• v.7 no.3
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• pp.29-35
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• 2011

This paper describes an internal flow characteristics of a fuel pressurized blower, used for 1kW domestic fuel cell system. To analyze the flow field inside the diaphragm cavity, compressible unsteady numerical simulation is introduced. SST model with scalable wall function is employed to estimate the eddy viscosity. Moving mesh system is applied to the numerical analysis for describing the volume change of a diaphragm cavity in time. Throughout numerical simulation with the modeling of the inlet and outlet valves in a diaphragm cavity, unsteady nature of an internal flow is successfully analyzed. Force variations on the lower plate of a diaphragm cavity are evaluated in time. It is found that the driving force at the suction stage of a diaphragm cavity is more necessary than that at the discharging stage.

• 한국전산유체공학회:학술대회논문집
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• 2005.10a
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• pp.81-85
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• 2005

Three-dimensional steady incompressible laminar entry flows in a square duct of $90^{\circ}$ bend are numerically simulated by a new solution code(PowerCFD) using unstructured cell-centered method. Solutions are obtained with three unstructured grid types of hexahedron, prism and hybrid at a Reynolds number, based on the hydraulic diameter and bulk velocity, of 790. Interesting features of the flow are presented in detail. Detailed comparisons between the computed solutions and the available experimental data are given mainly for the velocity distributions at cross-sections in a $90^{\circ}$ bend of a square duct with fully-developed entry flows. It is found that the code is capable of producing the nature of laminar flow in curved square duct with no grid type dependency.

• Management Science and Financial Engineering
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• v.3 no.1
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• pp.15-38
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• 1997

In a flexible manufacturing system, a cell controller is able to identify and evaluate a number of alternative decisions to meet the objectives set by the factory level controller. In this paper, a Petri net-based cell controller is presented to accomplish this task. A static model is developed by using the Integrated Computer Aided Definition(IDEF0) method to represent clear functional relationships among the objects of the system. Based on the static model, two Petri net models are developed for the physical part flow and for the information flow. Multiple decision alternatives are generated from the physical part flow model and are synchronized with the information flow model for execution of the selected alternative.

• Bulletin of the Korean Chemical Society
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• v.28 no.3
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• pp.403-407
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• 2007

The anodic degradation of benzoquinone(BQ), a model compound for wastewater treatment was carried out using a home-made flow-through electrochemical cell with carbon fibers. To optimize the controlled current electrolysis condition of an aqueous BQ solution, the experimental variables affecting the degradation of BQ, such as the applying current, pH, reaction time, and flow rate of the BQ solution were examined. The degradation products of the oxidation reaction were identified by High Performance Liquid Chromatography and Inductively Coupled Plasma Atomic Emission Spectrometer. Low molecular weight aliphatic acids, and CO2 were the major products in this experiment. The removal efficiency of BQ from the solution increased with the applying current and time. 99.23% of 1.0 × 10-2 M BQ was degraded to aliphatic acids and CO2 when the applying current is 175 mA in a 12 hr electrolysis.

• Journal of the Semiconductor & Display Technology
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• v.10 no.2
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• pp.109-113
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• 2011

Hot air flow simulations of the wet station dryer module for the solar cell cleaning were conducted. Air incident angles such as to the substrates ($45^{\circ}$), to the bottom ($90^{\circ}$), and to the wall ($135^{\circ}$) were considered. Based on the simulated velocity and temperature profiles, appropriate incident angle was proposed, and it was well matched to experimental results. Additionally, uniform and non-uniform air hole sizes of the tube were compared for the uniform air flow distribution through the batch.

• Journal of Veterinary Clinics
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• v.38 no.1
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• pp.36-40
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• 2021

A 12-year-old dog was referred due to multiple superficial lymphadenopathy. On cytology, each lymph node showed different cell populations where some of them consisted of intermediate to large lymphocytes with frequent Mott cells. Presence of Mott cells along with immature lymphocytes made the cytological diagnosis challenging, and therefore, supplementary diagnostic tests including PCR for Antigen Receptor Rearrangement (PARR) assay and flow cytometry were performed. This case report illustrates the value of flow cytometry in the diagnosis of lymphadenopathy with ambiguous cytologic findings.

• Journal of Mechanical Science and Technology
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• v.14 no.8
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• pp.861-866
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• 2000

Flow induced shape change is important for spatial interpretation of vascular response and for understanding of mechanotransduction in a single cell. We investigated the possible shapes of endothelial cell (EC) in a mathematical model and compared these with experimental results. The linearized analytic solution from the sinusoidal wavy wall and Stokes flow was applied with the constraint of EC volume. The three dimensional structure of the human umbilical vein endothelial cell was visualized in static culture or after various durations of shear stress (20 $dyne/cm^2$ for 5, 10, 20, 40, 60, 120min). The shape ratio (width: length: height) of model agreed with that of the experimental result, which represented the drag force minimizing shape of stream-lining. EC would be streamlined in order to accommodate to the shear flow environmented by active reconstruction of cytoskeletons and membranes through a drag force the sensing mechanism.

• 한국신재생에너지학회:학술대회논문집
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• 2008.05a
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• pp.538-541
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• 2008

This study considers the feasibility of the concentration control of the feul and air by oscillating flow in the channel of Fuel Cells. Fuel Cell Stack performance is largely influenced by the fuel and air concentration. If the fuel and air concentration is lower than stoichiometry 1.25 of the fuel and 2.5 of the air, its performance deteriorates seriously because of the fuel and air starvation. In this respect the optimization of the fuel and air concentration is crucially important to maximize fuel cell stack performance. In this work, the effects of oscillating actuation are studied to control the concentration. Two important nondimensional parameters are introduced, each of which represents either the oscillating frequency or the oscillating amplitude. It is shown how these factors affect the stack performance and the efficiency of the fuel cell stack stack.