• Journal of Korean Society on Water Environment
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• v.31 no.3
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• pp.328-333
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• 2015

In this study, Mg-Sericite was used as a flocculant to harvest freshwater microalgae, Chlorella. vulgaris. Mg-Sericite separated successfully >95% of the C. vulgaris at the following optimal parameters: Mg/Si mol ratio 1.25 to 1.50, mixing time of 20 min and settling time of 20 min. The harvesting efficiency was pH dependent. The highest harvesting efficiency ($99{\pm}0.3%$) was obtained at Mg/Si mol ratio 1.5 and pH 9 to 11. These results indicated that a biopolymer, Mg-Sericite, can be a promising flocculant due to its high efficiency, low dose requirements, short mixing and settling times. This harvesting method is helpful to lower the production cost of algae for biodiesel.

• The Korean Journal of Food And Nutrition
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• v.12 no.3
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• pp.240-245
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• 1999

The purpose of this study was to develop the new microbial bioflocculant available in a food and fer-mentation industal. This study was reported the results of the composition for optimum culture medium and elemental characteristic of crude purification bioflocculant following the previous report(I). The maximum production of the flocculant from Bacillus megaterium was observated in the culture medium containing 2% sucrose 0.3% NaNO3 0.01% tryptone 0.01% beef extract 0.05% MgSO4 ·7 H2O 0.005% CaCO3 Addition of the sucrose as carbon sources and inorganic salt such as MgSO4, CaCO3 significantly increased the production of flocculant more than nitrogen sources. In the result of color reaction of the crude purified bioflocculant it was investgated that anthrone was positive and benedict burette and nin-hydrin was negative. These result were indicated that the flocculant produced from Bacillus megaterium was a kind of exopolysaccharide.

• Journal of Korean Society of Environmental Engineers
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• v.30 no.8
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• pp.839-846
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• 2008

The flocculation characteristics of polyacrylamide base flocculants were estimated to reduce the moisture content of the dehydrated cakes. The dewaterability for sewage sludge was found to have a marked effect depending on the flocculant type, agitating speed and time, kind of dissolution water, etc. The optimal agitating speed and time were 700 rpm and 3 sec, respectively, in this experimental condition. and the dewaterability was proportion to the agitating speed upto 700rpm. When recycle water as the dissolution water was used, the solution viscosity of all kind of flocculants was decreased. However, the change of its viscosity are not proportioned to the dewaterabilities for each flocculant. Flocculation system of combinations of the first and sencond flocculation using single flocculant was investigated. Effects of the ratio of first and second dosage for dual flocculation on the dewaterability were also investigated. The optimum conditions of dual flocculation system are 75% and 50% as first dosages for low and high viscous flocculant for total dosage of common flocculation, respectively. Based on the results, an overall mechanism of dual flocculation system is proposed and it is envisaged that optimization of flocculation processes in this way can result in considerable savings in cost.

• Journal of the Korean Society of Food Science and Nutrition
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• v.24 no.5
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• pp.790-795
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• 1995

Flocculant-producing microorganisms were isolated from soil samples using kaoline as the flocculating test material. One strain that had high flocculating activity among them was selected and identified as Arcuadendron sp. TS-49. The favorable medium for production of the flocculant was 3% glucose, 0.2% yeast extract, 0.1% $NH_4Cl$, 0.01% $MgSO_4$, and 0.05% $MnSO_4$ in 150ml of T.W. with initial pH 7.0.The optimum culture temperature and pH were $30^{\circ}C$ and pH 7.0, respectively. The flocculant activity was observed most highly after 4 to 5 days of cultivation at the optimum condition and decreased significantly with the lapse of cultivation time. The flocculant was produced constituently and seemed to be degraded for ressimilation during cultivation. The productivity achieved by this system was about ten-fold higher than that of scrrening mediuim. This bioflocculant flocculated all tested solids, including various microorganisms and organic/inorganic compounds. Several qualitative analyses of the bioflocculant showed that it was a kind of glycoprotein containing sugars and protein.

• Journal of Environmental Science International
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• v.30 no.2
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• pp.135-143
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• 2021

This study executed evaluation of drying characteristics based on the polymer injection rate (8%, 10% and 12%) and the drying method[NIF(near-infrared ray). According to this study analyzed VS, VS/TS, and calorific value compared with 'the auxiliary fuel standard of the thermoelectric power plant and the combined heat & power plant'. The results are as follows. In the case of NIR, the VS was slightly changed at the early stage of the material preheating period and the constant drying rate period with low moisture evaporation. But VS reduction was shown higher as moisture was dried. In the case of non-digested sludge with high VS content, the VS reduction rate by drying was shown lower than that of digested sludge. As the flocculant injection rate increased, the VS loss due th drying was found to be small. Also, the higher the flocculant injection rate was the longer the drying time. Especially, in the case of the NIR drying equipment, as the moisture content of sewage sludge decreased(moisture content 20~40%), the loss of net VS also showed a tendency to increase sharply. It is shown that the high calorific value according to the drying time of the non-digested sludge was changed from 590 kcaℓ/kg to 3,005 kcaℓ/kg and from 539 kcaℓ/kg to 2,796 kcaℓ/kg.

• Microbiology and Biotechnology Letters
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• v.23 no.6
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• pp.770-776
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• 1995

For the purpose of development of non-toxic and biodegradable flocculant, chitosan complex was isolated from Ganoderma lucidum wastes. The isolated complex was identified as the expected chitosan-glucan complex by IR specta. The complex was extracted by treatment of 50% NaOH solution at 120$\circ$C for 5 hrs, namely optimal condition and solubilized with 2% acetic acid for fur-ther use as flocculant. Preliminary experiments showed that the solubilized complex had higher flocculation activity of 1.3 fold than commercial chitosan at 400 mg/l concentration in soybean curd wastewater. Also the solubilized complex removed 83% of MLSS and 60% of COD in the soybean curd wastewater treated by photosynthetic bacteria, 50% of turbidity and 21% of MLSS in sugar industry wastewater, and 90% of turbidity and 89% of MLSS in alcohol fermentation wastewater. Bacterial cell flocculation activities of the solubilized chitosan-glucan complex were 89% in Bacillus subtilis broth, 81% in Streptococcus lactis broth, and more than 90% in Escherichia coli broth after standing for 2 days. The results reveal that chitosan-glucan complex from Ganoderma lucidum wastes can substitute for commercial chitosan as non-toxic and biodegradable flocculant.

• Journal of Environmental Science International
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• v.1 no.2
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• pp.93.1-104
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• 1992

Because of varied functions and characteristics, water soluble polymers have lone attracted much intrest in both academic and industrial fields, especially now in pollution problems such as wastewater treatment through ployrneric flocculants. In this regard, the main objective of this study is to synthesize polymeric floccuants containning hydrophobic group in presence of the potassium persulfate/sodium thiosulfate redox system and to estimate their flocculating ability on the kaolin suspension. In order to test the flocculating power of flocculant prepared on the kaolin suspension system, turbidity of the supernatant liquid, residence time, filtration rate of flocculated samples, have additionally been measured from the flocculation experiments and analysed In comparision with the theoretical background.

• Microbiology and Biotechnology Letters
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• v.24 no.6
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• pp.699-704
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• 1996

About 600 microorganisms isolated from soil, marsh, compost, etc. were examined for their flocculating ability in the kaolin suspension and swine wastewater. Among them, K-199 was the best producer of flocculant and was identified to be a species belong to the genus Corynebacterium. The maximum production of the flocculant from Corynebacterium sp. K-199 was observed in culture medium containing 2% glycerol, 0.4% peptone, 0.3% K$_{2}$HPO$_{4}$, 0.1% CaCO$_{3}$, 0.05% NaCl, 0.1% yeast extract, 0.1% MgSO$_{4}$,7H$_{2}$O and initial pH 7.5 when cultured with rotary shaker controlled at 25$\circ$C and 150 rpm. Under the optimal culture condition with jar fermentor, the maximum production was reached to flocculating activity of 780 units/ml after 4 days. From the results of the activity be fully maintained by periodate oxidization, it suggests that the activity is due to the protein.

• Journal of Environmental Science International
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• v.23 no.1
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• pp.105-112
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• 2014

With the incorporation of hydrogen peroxide($H_2O_2$) and NaLS as an initiator and emulsifier, acrylic acid monomer was graft copolymerized with sodium alginate prepared from alginic acid, and then the grafted copolymer was confirmed through IR spectrometer. When the amounts of acrylic acid increased the grafting also showed increasing trend. While the contents of hydrogen peroxide was varied in the aqueous sodium alginate solution, the grafting efficiency decreased gradually as the amounts of initiator increased.

• Microbiology and Biotechnology Letters
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• v.28 no.4
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• pp.233-238
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• 2000

To develop biodegradable and safe flocculant, Achromobacter sp. HY-66 were isolated from soil. The purified flocculant was composed of fructose and glucose in an approximate molar ratio of 1:24. Molecular weight was approximately 9$\times$105 dalton by Sepharose CL-4B gel filtration chromatography. Elemental analysis revealed that the contents of carbon, hydrogen and oxygen were 37.72%, 5.37% and 56.89%, respectively. The presence of O-H stretching, C-H streching, carboxy ester and C-H bending were confirmed by IR absorption spectrum analysis. We suggest that a bioflocculant produced from Achromobacter sp. YJ-66 is new and different from other bioflocculants.