• Korean Journal of Animal Reproduction
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• v.23 no.4
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• pp.313-321
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• 1999

The purpose of these study was to investigate the use of a glass micropipette (GMP) as a vessel for vitrification of bovine IVP blastocysts, to compare the post-thaw survival rates of bovine blastocysts frozen in GMP with those frozen in OPS that have been previously investigated, and to improve the hatching rate following vitrification with GMP method. The GMP vessel permits higher freezing and warming rate than the OPS due to the higher heat conductivity of the glass and lower mass of the solution that contains the embryos. Groups of three bovine IVP blastocysts were sequentially placed into vitrification solution before being loaded into either the OPS or GMP vessels and immersed into L$N_2$within 20 to 25 sec. Post-thaw blastocysts were serially washed in 0.25 and 0.15 M sucrose in HM and TCM-199 for each 5 min, respectively, and then cultured in TCM 199 supplemented with 10% FCS for 24 h. The rate of blastocyst re-expanding did not significantly different for OPS (75.9%) and GMP (80.0%) methods (P＞0.05). The hatching rates in OPS (34.1%) and GMP (37.5%) methods were significantly lower than that in control group (54.3%) (P＞0.05). In addition, the rate of blastocyst re-expanding was significantly lower if blastocysts were vitrified in the wide portion of the micropipette rather than the narrow portion of the micropipette (83.3 vs 56.7%) (P＞0.05), even though three blastocysts were loaded per vessel. The hatching rate in 0.05% pronase solution treatment for 30, 60 and 90 see (45.9, 54.7 and 57.5%) were significantly higher than that in control (35.0%), even though there was not significantly different between 30 see and control. These results indicate that both vitrification vessels can provide high survival rates of bovine IVP blastocysts. However, the GMP vessel has the advantage over the OPS, in that the former does not need a cap to protect the vessel from floating after immersion in L$N_2$. The location of the embryos (narrow or wide portion of immersion) were considered to be limiting factors to the viability of bovine IVP embryos. The exposing in 0.05% pronase solution for 60 or 90 see can increase hatching rates of post-thaw bovine IVP blastocysts.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.3 no.4
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• pp.235-243
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• 1991

This paper is concerned with developing infinite elements which are applicable to wave diffraction and radiation problems in the vertical plane. The near need region surrounding the solid body is modeled using conventional finite elements. but the far fold region is represented using the infinite elements developed in this study. The shape functions for the infinite elements are derived from the analytical eigenseries solution of the scattered waves in the far field region. The system matrices of the elements are constructed by performing the integration in the infinite direction analytically to achieve computational efficiency. Numerical analysis is carried out for two floating bodies with different cross-sectional shapes to prove the efficiency and validity of the elements. Numerical experiments are also performed to determine the suitable location of the infinite elements which directly affect accuracy and efficiency of the solution.

• Journal of Korean Society of Forest Science
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• v.74 no.1
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• pp.29-36
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• 1986

A method isolating Populus euramericana cv. I-214 mesophyll protoplasts was developed to facilitate application of genetic engineering techniques to this species. The suitable medium for shoot multiplication in vitro was MS basal medium with $0.1mg/{\ell}$ BAP. The effects of several factors influencing protoplast isolation could be evaluated quickly by using leaf in vitro and known volumes of maceration and washing media. The best yields of mesophyll protoplasts were obtained using leaves in vitro in 2.0% Cellulase R-10, 0.8% Macerozyme R-10, 1.2% Hemicellulase, 2.0% Driselase, 0.05% Pectolyase Y-23, and O.6M Mannitol in addition to DTT and MES buffer adjusted to pH 5.6. Over $2.4{\times}10^6$ protoplasts per gram of leaf were produced using these conditions. For protoplast purification, the most favorable sucrose concentration of floating solution was 0.6M after washing them with CPW solution. This method of screening factors affecting protoplast isolation could be applicable to other species.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.24 no.5
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• pp.543-551
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• 2011

The paper deals with the comparative study of design optimization based on various approximation techniques in strength design of riser support structure installed on floating production storage and offloading unit(FPSO) using offshore operation loading conditions. The design optimization problem is formulated such that structural member sizing variables are determined by minimizing the weight of riser support structure subject to the constraints of structural strength in terms of loading conditions. The approximation techniques used in the comparative study are response surface method based sequential approximate optimization(RBSAO), Kriging based sequential approximate optimization(KBSAO), and the enhanced moving least squares method(MLSM) based approximate optimization such as CF(constraint feasible)-MLSM and Post-MLSM. Commercial process integration and design optimization(PIDO) tools are employed for the applications of RBSAO and KBSAO. The enhanced MLSM based approximate optimization techniques are newly developed to ensure the constraint feasibility. In the context of numerical performances such as design solution and computational cost, the solution results from approximate techniques based design optimization are compared to actual non-approximate design optimization.

• Journal of the Society of Naval Architects of Korea
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• v.28 no.2
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• pp.104-117
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• 1991

When a body starts to move, the flow near the intersection point between a body and a free surface changes violently and rapidly in a very short initial time interval. This flow phenomena must be investigated whenever one treats the interaction between a body and a fluid, such as the motion of a floating body, sloshing in a tank, wave maker problem, entry of a body into a fluid etc.. Until Roberts(1987), it was widely accepted that a singularity exists at the intersection point. However, he showed that the singularity does not exist if a body moves non-impulsively. In this paper, an analytical solution cosistent for the case of impulsive motion of a body is obtained by including the effect of surface tension. From the characteristics of the newly obtained solution, a critical value associated with an oscillating phenomenon is found, and further more, it is shown that the oscillating phenomenon does not appear in the region where the distance form the intersection point is less than this critical value.

• Proceedings of the KSME Conference
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• 2007.05b
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• pp.3080-3085
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• 2007

Shape optimization of the 3-dimensional WIG airfoil with 3.0-aspect ratio has been performed by using the multi-objective genetic algorithm. The WIG ship effectively floating above the surface by the ram effect and the virtual additional aspect ratio by a ground is one of next-generation and cost-effective transportations. Unlike the airplane flying out of the ground effect, a WIG ship has possibility to capsize because of unsatisfying the static stability. The WIG ship should satisfy aerodynamic properties as well as a static stability. They tend to strong contradict and it is difficult to satisfy aerodynamic properties and static stability simultaneously. It is inevitable that lift force has to scarify to obtain a static stability. Multi-objective optimization technique that the individual objectives are considered separately instead of weighting can overcome the conflict. Due to handling individual objectives, the optimum cannot be unique but a set of nondominated potential solutions: pareto optimum. There are three objectives; lift coefficient, lift-to-drag ratio and static stability. After a few evolutions, the non-dominated pareto individuals can be obtained. Pareto sets are all the set of possible and excellent solution across the design space. At any selections of the pareto set, these are no better solutions in all design space

• Ocean Systems Engineering
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• v.5 no.4
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• pp.261-277
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• 2015

Internal solitary waves occur due to density stratification and are nonlinear in nature. These waves have been observed in many parts of the world including the South China Sea, Andaman Sea and Sulu Sea. Their effect on floating systems has been an emerging field of interest and recent offshore developments in the South China Sea where several offshore oil and gas discoveries are located have confirmed adverse effects including large platform motions and riser system damage. A valid numerical model conforming to the physics of internal waves is implemented in this paper and the effect on a spar platform is studied. The physics of internal waves is modeled by the Korteweg-de Vries (KdV) equation, which has a general solution involving Jacobian elliptical functions. The effects of vertical density stratification are captured by solving the Taylor Goldstein equation. Fully coupled time domain analyses are conducted to estimate the effect of internal waves on a typical truss spar, which is configured to South China Sea development requirements and environmental conditions. The hull, moorings and risers are considered as an integrated system and the platform global motions are analyzed. The study could be useful for future guidance and development of offshore systems in the South China Sea and other areas where the internal wave phenomenon is prominent.

• Journal of Ocean Engineering and Technology
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• v.17 no.2
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• pp.8-13
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• 2003

Soil, ground water, and sea bed are exposed to a continuous accumulation of polluted materials, causing serious environmental damage. It has been reported that such pollution causes a massive mortality of fish stock in rivers due to the resuspension of toxic chemicals, occurring during strong wind conditions. Therefore, it becomes apparent that there is an immediate demand for the restoration treatment of polluted river bed (or sea bed) sediment layers. Pollution levels of major rivers and ports, such as Paldang, Kyungan rivers, and Masan port, are becoming of great public concern, and are posing a serious environmental threat. In particular, the pollution of the Shi-hwa river has become a nation wide issue for the last few years. In spite of such public concern, the pollution levels of such rivers or ports are worsening everyday. In this study, an environmentally sound engineering package is introduced that helps to restore the polluted river bed or sea bed sediments. This engineering package consists of a suction facility, followed by a series of mechanical, chemical, and biological treatment units. The suction facility is designed to minimize secondary pollution that occurs from the resuspension of toxic materials during suction. The sea bed cleaning engineering package is designed to be installed on the top of a floating barge. Such a combination of environmental plant and shipbuilding technology provides a cost-effective solution, minimizing the transportation between suction and treatment facilities.

• Journal of Ocean Engineering and Technology
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• v.23 no.6
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• pp.53-60
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• 2009

Recently, since there are several problems in space, the infra-structure and the facilities in the contiguity of the existing harbors due to the trend of enlarging the container capacity of the large container vessel, a special floating platform named as the Mobile Harbor has been proposed conceptually as an effective solution of those problems. Two kinds of hull shapes, a conventional mono-hull type and a catamaran type, are proposed as midway feeders to transfer containers to the harbor on land from a large container ship on near shore. In this study, the motion response and mooring analysis are carried out for comparing the global performance of two types of Mobile Harbor. Robot arm mooring facility specially is devised and newly tried to use for the safe fixation of a large container ship and the Mobile Harbor on near shore. It would be expected for this comparison study to give a guideline to design the efficient hull form for a midway loader.

• 한국신재생에너지학회:학술대회논문집
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• 2011.05a
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• pp.167.2-167.2
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• 2011

Motions in nature, for example ocean wave, has been playing a significant role for generating electricity production in our modern life. This paper presents an innovative approach for electric power conversion of the vast ocean wave energy. Here, a floating-buoy wave energy converter (WEC) using hydrostatic transmission (HST), which is shortened as HSTWEC, is proposed and designed to enhance the wave energy harvesting task during all wave fluctuations. In this HSTWEC structure, the power take-off system (PTO) is a combination of the designed HST circuit and an electric generator to convert mechanical energy generated by ocean wave into electrical energy. Several design concepts of the HSTWEC have been considered in this study for an adequate investigation. Modeling and simulations using MATLAB/Simulink and AMESim are then carried out to evaluate these design concepts to find out the best solution. In addition, an adaptive controller is designed for improving the HSTWEC performance. The effectiveness of the proposed HSTWEC control system is finally proved by numerical simulations.