• Title/Summary/Keyword: fixing

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Analysis of Endospore-forming Bacteria or Nitrogen-fixing Bacteria Community Isolated from Plants Rhizosphere in Dokdo Island (독도서식 식물근권에서 분리한 포자형성세균과 질소고정세균의 군집구조 분석)

  • Jeon, Seon-Ae;Sung, Hye-Ri;Park, Yu-Mi;Pak, Jae-Hong;Ghim, Sa-Youl
    • Microbiology and Biotechnology Letters
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    • v.37 no.3
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    • pp.189-196
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    • 2009
  • Bacteria were isolated from roots of plants belonging to family Solanaceae and Gramineae, inhabited in Dokdo island. Fifty six endospore-forming bacteria grown on tryptic soy broth (TSB) agar medium and 23 nitrogen-fixing bacteria (NFB) grown on nitrogen free agar medium were isolated, respectively. The isolates were partially identified by analyzing the 16S rDNA and categorized into phylogenetic groups. The 16S rDNA sequences of each identified isolates were compared with sequences of each type strains to analyze phylogenetic relationship by phylogenetic tree. As a result, endospore-forming bacteria and nitrogen-fixing bacteria were classified into 4 and 6 lineage groups, respectively. Among these isolated, 18 were presumed to be novel species candidates based on the similarity (lower than 98%) analysis of the l6S rDNA sequences.

Steering Gaze of a Camera in an Active Vision System: Fusion Theme of Computer Vision and Control (능동적인 비전 시스템에서 카메라의 시선 조정: 컴퓨터 비전과 제어의 융합 테마)

  • 한영모
    • Journal of the Institute of Electronics Engineers of Korea SC
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    • v.41 no.4
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    • pp.39-43
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    • 2004
  • A typical theme of active vision systems is gaze-fixing of a camera. Here gaze-fixing of a camera means by steering orientation of a camera so that a given point on the object is always at the center of the image. For this we need to combine a function to analyze image data and a function to control orientation of a camera. This paper presents an algorithm for gaze-fixing of a camera where image analysis and orientation control are designed in a frame. At this time, for avoiding difficulties in implementing and aiming for real-time applications we design the algorithm to be a simple closed-form without using my information related to calibration of the camera or structure estimation.

Isolation of Nitrogen-Fixing Bacteria from Gramineous Crops and Measurement of Nitrogenase Activity (벼과식물로부터 질소고정균의 분리와 Nitrogenase 활성 측정)

  • 최은화;이상은;윤기순;권덕기;손재근;박승환;한명숙;김사열
    • Microbiology and Biotechnology Letters
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    • v.31 no.1
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    • pp.18-24
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    • 2003
  • For researching nitrogen-fixing bacteria associated with gramineous crops, we collected growing roots of rices, wheats, oats, barleys, ryes, and maizes at 19 sites of southern Korean peninsula. Endophytes and free living bacteria were isolated from those crop roots. Sixty-three isolates were classified on the basis of different morphology, size, color, host of colony, and the 16S rDNAs sequence. The analyses of PCR amplification for nifH gene and nitrogenase activity assay, revealed that all isolates contained nitrogen-fixing abilities. In addition, most of them have cellulase activity which is one of the common features of endophytic bacteria from plant.

A Study of Fatigue Analysis for the Turntable Fixing Bolts Subjected to Mixed Load (혼합하중을 받는 선회대 고정볼트의 피로분석에 관한 연구)

  • Choi, Dong-Hoon;Lee, Do-Nam;Kim, Jae-Hoon
    • Journal of the Korean Society of Safety
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    • v.37 no.5
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    • pp.1-6
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    • 2022
  • In this study, to confirm the effects of the mixed load of normal and shear forces acting on a fixing bolt, fatigue design criteria were developed by varying the loading angle and conducting tensile and fatigue tests. After evaluating and comparing the test results under different loading angles, the evaluation criteria were selected. These evaluation criteria were then applied to develop the design criteria. An Arcan fixture was designed and manufactured to simultaneously apply a mixed load of normal and shear forces to the fixing bolt of a turntable, and a fatigue test was conducted. S-N diagrams for various loading angles were obtained, and a 1% P-S-N diagram of failure probability was determined using statistical processing techniques. Our results show that failures of the fixing bolt can be prevented using these diagrams as a basis for developing fatigue design criteria.

MtMKK5 inhibits nitrogen-fixing nodule development by enhancing defense signaling

  • Hojin Ryu
    • Journal of Plant Biotechnology
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    • v.49 no.4
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    • pp.300-306
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    • 2022
  • The mitogen-activated protein kinase (MAPK) signaling cascade is essential for a wide range of cellular responses in plants, including defense responses, responses to abiotic stress, hormone signaling, and developmental processes. Recent investigations have shown that the stress, ethylene, and MAPK signaling pathways negatively affect the formation of nitrogen-fixing nodules by directly modulating the symbiotic signaling components. However, the molecular mechanisms underlying the defense responses mediated by MAPK signaling in the organogenesis of nitrogen-fixing nodules remain unclear. In the present study, I demonstrate that the Medicago truncatula mitogen-activated protein kinase kinase 5 (MtMKK5)-Medicago truncatula mitogen-activated protein kinase 3/6 (MtMPK3/6) signaling module, expressed specifically in the symbiotic nodules, promotes defense signaling, but not ethylene signaling pathways, thereby inhibiting nodule development in M. truncatula. U0126 treatment resulted in increased cell division in the nodule meristem zone due to the inhibition of MAPK signaling. The phosphorylated TEY motif in the activation domain of MtMPK3/6 was the target domain associated with specific interactions with MtMKK5. I have confirmed the physical interactions between M. truncatula nodule inception (MtNIN) and MtMPK3/6. In the presence of high expression levels of the defense-related genes FRK1 and WRKY29, MtMKK5a overexpression significantly enhanced the defense responses of Arabidopsis against Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). Overall, my data show that the negative regulation of symbiotic nitrogen-fixing nodule organogenesis by defense signaling pathways is mediated by the MtMKK5-MtMPK3/6 module.

Isolation of N2-fixing Microorganism from the Root of Non-leguminous Crops (수종(數種)의 비두과(非豆科) 식물(植物)로 부터의 질소고정균(窒素固定菌)의 분리(分離)에 관(關)한 연구(硏究))

  • Ahn, Sang-Bae;Gamo, H.;Yuk, Chang-Soo
    • Korean Journal of Soil Science and Fertilizer
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    • v.23 no.1
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    • pp.67-72
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    • 1990
  • A number of nitrogen-fixing microorganisms were isolated from roots of non-legumious crops and evaluated for their nitrogen-fixing ability on their host crops. The results are summarized as follows: 1. Among nitrogen-fixing isolates obtained from 9 different crops, several isolates showed relatively higher level of acetylene reduction activity on Spinach, Chinese cabbage, Cucumber, mustard and Egg plant. 2. When the isolates were inoculated into 4 host crops, a number of isolates were found to grow well on Spinach, Chinese cabbage and Cucumber, but particularly well on the former. 3. Results of this study suggest that nitrogen-fixing a symbiotic microorganisms are also present and able to grow well on roots of non-legumious crops, and stimulate plant growth by promoting growth and differentiation of roots.

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Fixing Behaviors of Dimethylamino Anthraquinone Disperse Dyes and Monochlorotriazinyl Azo Reactive Dyes on P/C Blended Fabrics in One-Step Printing (디메틸아미노안트라퀴논계 분산염료와 아조계 모노클로로트리아진형 반응염료에 의한 P/C혼방직물의 일단계 날염에 있어 고착거동)

  • Park, Geon-Yong;Seo, Gi-Sung
    • Textile Coloration and Finishing
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    • v.19 no.3
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    • pp.18-25
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    • 2007
  • The fixing behaviors of anthraquinone disperse dyes containing dimethylamino substituent, such as C. I. Disperse Violet 26(D.V.26) and C. I. Disperse Blue 14(D.V.14), or containing diamino substituent, such as C. I. Disperse Blue 73(D.B.73), and monochlorotriazinyl azo reactive dyes, such as C. I. Reactive Orange 13(R.O.13), C. I. Reactive Red 3(R.R.3). C. I. Reactive Yellow 2(R.Y.2) on polyester/cotton blend(P/C) fabrics were examined for the one-step printing of P/C fabrics. The high temperature steaming of $175^{\circ}C$ is the most satisfactory fixing method for P/C one-step printing with above disperse and reactive dyes among the four different fixing methods: $175^{\circ}C$ steaming, $102^{\circ}C$ steaming${\rightarrow}175^{\circ}C$ steaming, $190^{\circ}C$ thermosol, $102^{\circ}C$ steaming${\rightarrow}190^{\circ}C$ thermosol. $190^{\circ}C$ thermosol is unfit to fix R.R.3 and R.Y.2 whose heat stability is poor. It was found that D.V.26 and D.B.14 containing dimethylamino substituent are unstable for heat and alkali, but D.B.73 is stable for them to print P/C blend fabrics with R.O.13 which is also stable for heat. Therefore we found that D.B.73, R.O.13 and a pair of D.B.73 and R.O.13 were very suitable for one-step printing of P/C blend fabrics.

PDM ZCS Resonant High Frequency Inverter for Induction Heated Roller

  • Kang, Shin-Chul
    • Journal of information and communication convergence engineering
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    • v.6 no.3
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    • pp.301-304
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    • 2008
  • This paper presents a lossless inductor snubber-assisted series resonant high frequency inverter using the trench gate IGBTs for the electromagnetic induction eddy current-heated fixing roller in copy and printing machines in a new generation. This soft switching high-frequency inverter can completely achieve zero current soft switching (ZCS) commutation mode transitions and wide power regulation under its constant frequency pulse density modulation (PDM) scheme. Its transient and steady state operating principle is originally presented for a constant frequency PDM strategy under a ZCS operation, together with its output power regulation characteristics-based on the pulse density modulation. The effectiveness of this soft switching high-frequency inverter is confirmed for the fixing equipment with the induction-heated fixing roller.

A Study on Modeling the Screw for Permanent Fixing and the Simulation of Fixing force (영구 체결용 나사 모델링과 체결력 시뮬레이션에 관한 연구)

  • Lee, S.S.;Prak, J.H.;Kim, M.J.;Park, J.B.;Jeon, E.C.
    • Proceedings of the KSME Conference
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    • 2001.11a
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    • pp.827-833
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    • 2001
  • This study is an introduction of ADS in which we have written out programs which can design three-dimensional machine parts and reduce the required time for design them comparing with other CADs. Among the machine parts, screws are commonly used in joining one part to another because of easy handing but welding and rivet are used to join permanently them even thought hard operation. Consequently, we have developed the screw for permanent fixing using stairs type screw threads, which is considered as the main machine parts in joining. For that, we designed permanent fastened screws according to the different rotation angle using VisualLISP and transferred it to ANSYS, structural solution program, to find out allowance load, maximum equivalent stress and where it occur.

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A Study on Pre-Red Mud and Bio-Solids Applicability as Soil Stabilizer (Pre-Red Mud 및 Bio-Solids의 토양 안정화제 활용 가능성에 대한 연구)

  • Yang, Joo-Kyung;Kang, Seon-Hong
    • Journal of Korean Society of Water and Wastewater
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    • v.25 no.3
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    • pp.419-428
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    • 2011
  • Recycling as a stabilizer of industrial by-product can be terms of the proper handling of industrial by-product and positive side in terms of recycling of waste. This study was performed to evaluate has the possibility as stabilizer by primary processing Pre-Red Mud and Bio-Solids which are generated as waste in soils contaminated with heavy metals and compared the efficiency with steel slug being applied in an existing site. In evaluation of the arsenic-fixing ability of stabilizer in batch test, Bio-Solids have the similar arsenic-fixing ability with Pre-Red Mud, which shows 17% h igher arsenic-fixing ability than PS Ball. Since the stabilization periods using Bio-Solids and Pre-Red Mud are faster than the PS Ball, they seems to be better stabilizer than PS Ball to decrease the leaching of arsenic in contaiminated soil.